Ayçiçeği Helianthus annuus L. ’nin Olgunlaşmamış Embriyo ve Kotiledon Eksplantlarından Adventif Sürgün Rejenerasyonu

Öz

Ayçiçeğinin Ekiz genotipinden seçilen 10 hattın olgunlaşmamış embriyo ve kotiledon eksplanları 20 mg/l sukroz ve farklı oranlarda kinetin ve naftalen asetik asit NAA içeren Murashige and Skoog MS ortamında kültüre alınmıştır. Denemelerde 1. hafta sonunda rejenerasyonu olumsuz etkileyen kaçak kökler steril koşullarda eksplantlardan uzaklaştırılmıştır. Özellikle olgunlaşmamış embriyo eksplantında çalışılan tüm hatlarda ve ortamlarda kallus ve sürgün oluşumu görülürken olgunlaşmamış kotiledon eksplantında tüm ortamlarda iyi bir kallus oluşumu gerçekleşmiş ancak daha az sürgün elde edilmiştir. Kallus çapı ve ağırlığının sürgün gelişimine etkisi olmadığı görülmüştür. Bitki doku kültürü çalışmalarında kullanılan eksplantların sürgün gelişimi için önemli faktörlerden birisi olduğu gözlenmistir

Anahtar Kelimeler

• Helianthus annuus L.
• ayçiçegi
• olgunlaşmamış embriyo
• olgunlaşmamış kotiledon
• in vitro

Adventitious Shoot Regeneration from Immature Embryo and Cotyledon Explants of Sunflower Helianthus annuus L.

Abstract

Immature embryo and cotyledons of 10 lines of sunflower Helianthus annuus L were cultured on Murashige and Skoog MS medium supplemented with 20 mg/l sucrose and different concentrations of Kinetin and α Naphthalene acetic acid NAA . Development of precocious roots was observed after one week of culture, which were removed aseptically to induce callus and shoot regeneration. Induction of healthy shoots was observed on cotyledon and embryo explants on MS medium containing any concentration of kinetin and and NAA. This is an important development in the tissue culture research of sunflower

Keywords

• Helianthus annuus L
• sunflower
• immature embryo
• immature cotyledons
• in vitro

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