Bazı Ayva Cydonia oblonga Mill. Genotiplerinde Yaprak Disklerinden Sürgün Organogenesisi

Öz

Bu çalışmanın amacı, bazı ayva Cydonia oblonga Mill. genotiplerinde yaprak disklerinden in vitro sürgün organogenesisini uyarmaktır. Çalışmada bitkisel materyal olarak, Quince A bodur , S.Ö. Sabahattin Özbek 39-200 bodur , S.Ö. 18-82 orta kuvvetli ve S.Ö. 58-315 kuvvetli klon anaçları ile Eşme, Limon ve Çukurgöbek çeşitleri kullanılmıştır. Organogenesis için iki denemede TDZ + NAA kombinasyonları ya da BA + NAA, AgNO3 ve putrasin uygulamalarının etkileri araştırılmıştır. Sürgün organogenesisi sadece 0.33 mg/l TDZ + 0.5 mg/l NAA + 2.0 mg/l AgNO3 içeren MS Murashige ve Skoog temel besin ortamında önemli düzeyde uyarılmıştır. Bu uygulama ile sürgün oluşturma oranı Quince A’da %80.0, S.Ö. 39-200’de %56.7, S.Ö. 18-82’de %17.5, S.Ö. 58-315’de %37.5, Eşme’de %36.7, Limon’da %40.0 ve Çukurgöbek’de %43.3 olarak belirlenmiştir. Ortalama sürgün sayısı ise genotiplere göre 0.4-2.7 adet/disk arasında değişmiştir

Anahtar Kelimeler

• Ayva
• Cydonia oblonga Mill.
• in vitro
• organogenesis
• yaprak diski
• sürgün

Shoot Organogenesis From Leaf Discs in Some Quince Cydonia oblonga Mill. Genotypes

Abstract

The objective of this study was to stimulate organogenesis of in vitro shoots from leaf discs in some quince Cydonia oblonga Mill. genotypes. Quince A dwarf , S.Ö. Sabahattin Özbek 39-200 dwarf , S.Ö. 18-82 semi dwarf and S.Ö. 58-315 vigorous clonal rootstocks, and Eşme, Limon, and Çukurgöbek cultivars were used as plant materials. Effects of TDZ + NAA combinations or BA + NAA, AgNO and putrescine treatments were investigated in two experiments for organogenesis. Shoot organogenesis was significantly induced on MS Murashige and Skoog basal medium containing 0.33 mg/l TDZ + 0.5mg/l NAA + 2.0 mg/l AgNO3. The rate of shoot formation was 80.0% in Quince A, 56.7% in S.Ö. 39-200, 17.5% in S.Ö. 18-82, 37.5% in S.Ö. 58-315, 36.7% in Eşme, 40.0% in Limon, and 43.3% in Çukurgöbek. The average shoot number per leaf disc was changed between 0.4 and 2.7 among genotypes

Keywords

• Quince
• Cydonia oblonga Mill.
• in vitro
• organogenesis
• leaf disc
• shoot

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