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Türkiye’den İzole Edilen Entomopatojen Nematodlar, Steinernema weiseri ve S. feltiae Rhabditida: Steinernematidae ’nin Bazı Biyolojik Özelliklerinin Karşılaştırılması

Year 2006, , 340 - 344, 01.11.2006
https://doi.org/10.1501/Tarimbil_0000000397

Abstract

Türkiye’de ilk kez entomopatojen nematod Steinernema weiseri Ankara, Hacettepe Üniversitesi Beytepe Kampüsünden ve S. feltiae Rhabditida: Steinernematidae Ankara Üniversitesi kampüsünden izole edilmiştir. Bu çalışmada her iki entomopatojen nematodun değişik koşullarda etkinlik ve üreme kapasiteleri karşılaştırılmıştır. Üreme kapasiteleri Balmumu Güvesi, Galleria mellonella L. Lep: Pyralidae ‘nın son dönem larvaları üzerine 10, 15 ve 20 ºC sıcaklıklarda 10, 50 ve 100 adet infektif larva nematodun uygulanması ile belirlenmiştir. Konukçu larva içerisinde üreyerek kadavradan dışarı çıkan her iki nematoda ait yeni generasyon jüvenilleri sayılmıştır. Tüm deneme koşullarında S. weiserii’nin S. feltiae’ye göre daha fazla üreme kapasitesine sahip olduğu belirlenmiştir. G. mellonella’nın son dönem larvaları üzerinde her iki nematodun etkinliği penetrasyondan 48 saat sonra LD50 olarak belirlenmiştir. Bu sonuçlara göre S. weiseri’nin, S. feltiae’ye göre daha etkili olduğu ve biyolojik mücadelede kullanımının daha yararlı olabileceği bulunmuştur

References

  • Aguillera, M. M. 1992. Steinernema scapterisci Nguyen and Smart, 1990: Bacterial associates, cultures and pathogenicity. Dissertation to the Graduate Scholl of the University of Florida in Partial Fulfillment of the Required for the Degree of Doctor of Philisophy, Florida, USA
  • Akhurst, R. J. 1982. A Xenorhabdus sp. (Eubacteriales: Enterobacteriaceae) symbiotically associated with Steinernema kraussei (Nematoda: Steinernematidae). Revue Nematol. 5 (2): 227-280.
  • Bhatnagar, A., V. Shinde and S. S. Bareth. 2004. Evaluation of entomopathogenic nematodes against whitegrub, Maladera insanabilis Brenske. Int. J. Pest Manag. 50 (4): 285-289.
  • Boff, M. I. C., G. L. Wiegers and P. H. Smits. 2000. The influence of storage temperature and time on infectivity and reproduction of Heterorhabditis megidis (strain NLH-E87.3). IOBC WPRS Bull. 23(2): 53-60.
  • Campbell, J. F. and R. Gaugler. 1993. Nictation behaviour and its ecological implications in the host search strategies of entomopathogenic nematodes (Heterorhabditidae and Steinernematidae). Behaviour. 126: (3-4).
  • Caroli, L., I. Glazer and R. Gaugler. 1996. Entomopathogenic nematode infectivity assay: comparison of penetration rate into different hosts. Biocontrol Sci. Technol. 6:227- 233.
  • Ehlers, R. U. 1996. Current and future use of nematodes in biocontrol: practice and commercial aspects with regard to regulatory policy issues. Biocontrol Sci. Technol. 6: 303-316.
  • Fenton, A. and P. J. Hudson. 2002. Optimal infection strategies: should microparasites hedge their bets? Oikos. 96: 92-101.
  • Fenton, A. and S. A. Rands. 2004. Optimal parasite infection strategies: a state-dependent approach. Inter. J. Parasit. 34: 813-821.
  • Finney, D. J. 1971. Probit Analyse. Cambridge Univ. Press, London, England.
  • Gaugler, R. and H. K. Kaya. 1990. Entomopathogenic nematodes in biological control, CRC Press, Boca Raton, FL. 344
  • Gaugler, R. 1988. Ecological considerations in the biological control of the soil inhabiting insects with entomopathogenic nematodes. Agric. Ecosyst. Environ. 24: 351-360.
  • Gaugler, R. 2002. Entomopathogenic nematology. CABI Publishing, Wallingford, UK.
  • Griffin, C. T., M. J. Downes and W. Block. 1990. Tests of antarctic soils for insect parasitic nematodes. Antarct. Sci. 2: 221-222.
  • Hazır, S., S. P. Stock and N. Keskin. 2003. A new entomopathogenic nematode, Steinernema anatoliense n. sp. (Rhabditida: Steinernematidae), from Turkey. Syst. Parasitol. 55: 211-220.
  • Hominick, W. M. 2002. Biogeography Entomopathogenic nematology (ed R. Gaugler). CABI Publishing, Wallingford, UK.
  • Hominick, W. M., A. P. Reid, D. A. Bohan, B. R. Briscoe. 1996. Entomopathogenic nematodes: biodiversity, geographical distribution and the convention on biological diversity. Biocontrol Sci. Technol. 6: 317-331.
  • Hominick, W. M., A. P. Reid, D. J. Hunt and B. R. Briscoe. 2000. Systematics and biogeography of entomopathogenic nematodes pp. 17-28. Ed.: Griffin, C. T., A. M. Burnell, M.J. Downes and R. Mulder. COST 819 Developments in Entomopathogenic Nematode/Bacterial Research. European Commission, DG XII, Luxembourg.
  • Hui, E. and J. M. Webster. 2000. Influence of insect larvae and seedling roots on the host-finding ability of Steinernema feltiae (Nematoda: Steinernematidae). J. Invertebr. Pathol. 75: 152-162.
  • Kaya, H. K. and R. Gaugler. 1993. Entomopathogenic nematodes. Annu. Rev. Entomol. 38: 181-206.
  • Kepenekçi, İ., N. E. Barbaroğlu, G. Öztürk and S. Halıcı. 1999. Türkiye için yeni bir entomopatojen nematod; Heterorhabditis bacteriophora Poinar 1976 (Rhabditida: Heterorhabditidae). Türkiye 4. Biyolojik Mücadele Kongresi Bildirileri, Entomoloji Derneği Yayınları: 9.
  • Molyneux, A. S., R. A. Bedding and R. J. Akhurst. 1983. Susceptibility of the sheep blowfly Lucilia cuprina to various Heterorhabditis spp., Neoplactana spp. and an undescribed steinernematid (Nematoda). J. Invertebr. Pathol. 42: 1-7.
  • Mracek, Z., D. Sturhan and A. Reid. 2003. Steinernema weiseri n. sp. (Rhabditida, Steinernematidae), a new entomopathogenic nematode from Europe. Syst. Parasitol. 56: 37-47.
  • Nguyen, K. B., D. I. Shapiro-Ilan, R. J. Stuart, C. W. McCoy, R. R. James and B. Adams. 2004. Heterorhabditis mexicano n. sp. (Heterorhabditidae: Rhabditida) from Tamaulipos, Mexico with morphological studies of bursa of Heterorhabditis spp. Nematol. 6: 231-244.
  • Özer, N., N. Keskin and Z. Kırbaş. 1995. Occurence of entomopathogenic nematodes (Steinernematidae: Heterorhabditidae) in Turkey. Nematologica 41: 639- 640.
  • Phan, K. L., N. C. Nguyen and M. Moens. 2001a. Steinernema sangi sp. n. (Rhabditidae, Steinernematidae) from Vietnam. Russ. J. Nematol. 9:1-17.
  • Phan, K. L., N. C. Nguyen and M. Moens. 2001b. Steinernema loci sp. n. and Steinernema thanhi sp. n. (Rhabditidae, Steinernematidae) from Vietnam. Nematol. 3: 503-514.
  • Poinar, G. O. 1990. Biology and taxonomy of Steinernematidae and Heterorhabditidae. (R. gaugler and H. K. Kaya eds), Entomopathogenic nematodes in biological control, CRC Press, Boca Raton, FL, p. 23- 58.
  • Qiu, L., Y.U. Fang, Y. Zou, Y. Pang and K. B. Nguyen. 2004. Steinernema guangdongense sp. n. (Nematoda: Steinernematidae), a new entomopathogenic nematode from southern China with a note on S. serratum (nomen nudum). Zootaxa 704: 1-20.
  • Samish, M. and I. Glazer. 1992. Infectivity of entomopathogenic nematodes (Steinernematidae and Heterorhabditide) to female ticks of Boophilus annulatus (Arachnida: Ixodidae). J. Med. Entomol. 29 (4): 614- 618.
  • Statistica, 1991. Complete Statistical System by StatSoft, Inc. 2325 East 13th Street, Tulsa, OK.
  • Susurluk, A., I. Dix, E. Stackebrandt, O. Strauch, U. Wyss and R. U. Ehlers. 2001. Identification and ecological characterisation of three entomopathogenic nematode- bacterium complexes from Turkey. Nematol. 3 (8): 833- 841.
  • Susurluk, I. A. 2005. Establishment and persistence of the entomopathogenic nematodes, Steinernema feltiae and Heterorhabditis bacteriophora. Dissertation zur Erlangung des Doktorgrades der Agrar-und Ernährungswissenschaftlichen Fakultät der Christian- Albrechts-Universität zu Kiel-Germany.
  • Wiesner, A. 1993. Die Induktion der Immunabwehr eines Insekts (Galleria mellonella, Lepidoptera) durch synthetische Materialien und arteigene Haemolymphfaktoren. PhD Thesis. Berlin.

Comparison of Some Biological Characterizations of the Entomopathogenic Nematodes, Steinernema weiseri and S. feltiae Rhabditida: Steinernematidae , Isolated in Turkey

Year 2006, , 340 - 344, 01.11.2006
https://doi.org/10.1501/Tarimbil_0000000397

Abstract

The entomopathogenic nematodes, Steinernema weiseri, was firstly isolated in Beytepe Campus of Hacettepe University, in Ankara, Turkey and S. feltiae Rhabditida: Steinernematidae , which was found in Ankara University Campus. In the present study, reproduction capacities and effectiveness of the both entomopathogenic nematodes were compared in variable conditions. The reproduction capacities were examined at the following temperatures; 10, 15 and 20 ºC and the doses of 10, 50 and 100 infective juvenilesdauer juveniles DJs in the last instar larvae of greater wax moth, Galleria mellonella L. Lep: Pyralidae . New generation DJs of each species, which produce within the host larvae and emerging from cadaver were counted and the results showed that S. weiseri was more productive than S. feltiae in all experimental conditions. Effectiveness of the both species on the last instar G. mellonella larvae as LD50 was calculated 48 h after penetration. According to the results, S. weiseri is more effective than S. feltiae. The present results indicated that S. weiseri can be more useful for biological control

References

  • Aguillera, M. M. 1992. Steinernema scapterisci Nguyen and Smart, 1990: Bacterial associates, cultures and pathogenicity. Dissertation to the Graduate Scholl of the University of Florida in Partial Fulfillment of the Required for the Degree of Doctor of Philisophy, Florida, USA
  • Akhurst, R. J. 1982. A Xenorhabdus sp. (Eubacteriales: Enterobacteriaceae) symbiotically associated with Steinernema kraussei (Nematoda: Steinernematidae). Revue Nematol. 5 (2): 227-280.
  • Bhatnagar, A., V. Shinde and S. S. Bareth. 2004. Evaluation of entomopathogenic nematodes against whitegrub, Maladera insanabilis Brenske. Int. J. Pest Manag. 50 (4): 285-289.
  • Boff, M. I. C., G. L. Wiegers and P. H. Smits. 2000. The influence of storage temperature and time on infectivity and reproduction of Heterorhabditis megidis (strain NLH-E87.3). IOBC WPRS Bull. 23(2): 53-60.
  • Campbell, J. F. and R. Gaugler. 1993. Nictation behaviour and its ecological implications in the host search strategies of entomopathogenic nematodes (Heterorhabditidae and Steinernematidae). Behaviour. 126: (3-4).
  • Caroli, L., I. Glazer and R. Gaugler. 1996. Entomopathogenic nematode infectivity assay: comparison of penetration rate into different hosts. Biocontrol Sci. Technol. 6:227- 233.
  • Ehlers, R. U. 1996. Current and future use of nematodes in biocontrol: practice and commercial aspects with regard to regulatory policy issues. Biocontrol Sci. Technol. 6: 303-316.
  • Fenton, A. and P. J. Hudson. 2002. Optimal infection strategies: should microparasites hedge their bets? Oikos. 96: 92-101.
  • Fenton, A. and S. A. Rands. 2004. Optimal parasite infection strategies: a state-dependent approach. Inter. J. Parasit. 34: 813-821.
  • Finney, D. J. 1971. Probit Analyse. Cambridge Univ. Press, London, England.
  • Gaugler, R. and H. K. Kaya. 1990. Entomopathogenic nematodes in biological control, CRC Press, Boca Raton, FL. 344
  • Gaugler, R. 1988. Ecological considerations in the biological control of the soil inhabiting insects with entomopathogenic nematodes. Agric. Ecosyst. Environ. 24: 351-360.
  • Gaugler, R. 2002. Entomopathogenic nematology. CABI Publishing, Wallingford, UK.
  • Griffin, C. T., M. J. Downes and W. Block. 1990. Tests of antarctic soils for insect parasitic nematodes. Antarct. Sci. 2: 221-222.
  • Hazır, S., S. P. Stock and N. Keskin. 2003. A new entomopathogenic nematode, Steinernema anatoliense n. sp. (Rhabditida: Steinernematidae), from Turkey. Syst. Parasitol. 55: 211-220.
  • Hominick, W. M. 2002. Biogeography Entomopathogenic nematology (ed R. Gaugler). CABI Publishing, Wallingford, UK.
  • Hominick, W. M., A. P. Reid, D. A. Bohan, B. R. Briscoe. 1996. Entomopathogenic nematodes: biodiversity, geographical distribution and the convention on biological diversity. Biocontrol Sci. Technol. 6: 317-331.
  • Hominick, W. M., A. P. Reid, D. J. Hunt and B. R. Briscoe. 2000. Systematics and biogeography of entomopathogenic nematodes pp. 17-28. Ed.: Griffin, C. T., A. M. Burnell, M.J. Downes and R. Mulder. COST 819 Developments in Entomopathogenic Nematode/Bacterial Research. European Commission, DG XII, Luxembourg.
  • Hui, E. and J. M. Webster. 2000. Influence of insect larvae and seedling roots on the host-finding ability of Steinernema feltiae (Nematoda: Steinernematidae). J. Invertebr. Pathol. 75: 152-162.
  • Kaya, H. K. and R. Gaugler. 1993. Entomopathogenic nematodes. Annu. Rev. Entomol. 38: 181-206.
  • Kepenekçi, İ., N. E. Barbaroğlu, G. Öztürk and S. Halıcı. 1999. Türkiye için yeni bir entomopatojen nematod; Heterorhabditis bacteriophora Poinar 1976 (Rhabditida: Heterorhabditidae). Türkiye 4. Biyolojik Mücadele Kongresi Bildirileri, Entomoloji Derneği Yayınları: 9.
  • Molyneux, A. S., R. A. Bedding and R. J. Akhurst. 1983. Susceptibility of the sheep blowfly Lucilia cuprina to various Heterorhabditis spp., Neoplactana spp. and an undescribed steinernematid (Nematoda). J. Invertebr. Pathol. 42: 1-7.
  • Mracek, Z., D. Sturhan and A. Reid. 2003. Steinernema weiseri n. sp. (Rhabditida, Steinernematidae), a new entomopathogenic nematode from Europe. Syst. Parasitol. 56: 37-47.
  • Nguyen, K. B., D. I. Shapiro-Ilan, R. J. Stuart, C. W. McCoy, R. R. James and B. Adams. 2004. Heterorhabditis mexicano n. sp. (Heterorhabditidae: Rhabditida) from Tamaulipos, Mexico with morphological studies of bursa of Heterorhabditis spp. Nematol. 6: 231-244.
  • Özer, N., N. Keskin and Z. Kırbaş. 1995. Occurence of entomopathogenic nematodes (Steinernematidae: Heterorhabditidae) in Turkey. Nematologica 41: 639- 640.
  • Phan, K. L., N. C. Nguyen and M. Moens. 2001a. Steinernema sangi sp. n. (Rhabditidae, Steinernematidae) from Vietnam. Russ. J. Nematol. 9:1-17.
  • Phan, K. L., N. C. Nguyen and M. Moens. 2001b. Steinernema loci sp. n. and Steinernema thanhi sp. n. (Rhabditidae, Steinernematidae) from Vietnam. Nematol. 3: 503-514.
  • Poinar, G. O. 1990. Biology and taxonomy of Steinernematidae and Heterorhabditidae. (R. gaugler and H. K. Kaya eds), Entomopathogenic nematodes in biological control, CRC Press, Boca Raton, FL, p. 23- 58.
  • Qiu, L., Y.U. Fang, Y. Zou, Y. Pang and K. B. Nguyen. 2004. Steinernema guangdongense sp. n. (Nematoda: Steinernematidae), a new entomopathogenic nematode from southern China with a note on S. serratum (nomen nudum). Zootaxa 704: 1-20.
  • Samish, M. and I. Glazer. 1992. Infectivity of entomopathogenic nematodes (Steinernematidae and Heterorhabditide) to female ticks of Boophilus annulatus (Arachnida: Ixodidae). J. Med. Entomol. 29 (4): 614- 618.
  • Statistica, 1991. Complete Statistical System by StatSoft, Inc. 2325 East 13th Street, Tulsa, OK.
  • Susurluk, A., I. Dix, E. Stackebrandt, O. Strauch, U. Wyss and R. U. Ehlers. 2001. Identification and ecological characterisation of three entomopathogenic nematode- bacterium complexes from Turkey. Nematol. 3 (8): 833- 841.
  • Susurluk, I. A. 2005. Establishment and persistence of the entomopathogenic nematodes, Steinernema feltiae and Heterorhabditis bacteriophora. Dissertation zur Erlangung des Doktorgrades der Agrar-und Ernährungswissenschaftlichen Fakultät der Christian- Albrechts-Universität zu Kiel-Germany.
  • Wiesner, A. 1993. Die Induktion der Immunabwehr eines Insekts (Galleria mellonella, Lepidoptera) durch synthetische Materialien und arteigene Haemolymphfaktoren. PhD Thesis. Berlin.
There are 34 citations in total.

Details

Primary Language English
Journal Section Research Article
Authors

Alper Susurluk This is me

Publication Date November 1, 2006
Submission Date November 1, 2006
Published in Issue Year 2006

Cite

APA Susurluk, A. (2006). Comparison of Some Biological Characterizations of the Entomopathogenic Nematodes, Steinernema weiseri and S. feltiae Rhabditida: Steinernematidae , Isolated in Turkey. Journal of Agricultural Sciences, 12(04), 340-344. https://doi.org/10.1501/Tarimbil_0000000397

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