Frisol F, Promot ve Fluoresent Pseudomonaslar ı n Kavunda Fusarium oxysporum f. sp. melonis Irk 1,2' nin Neden Oldu ğ u Solgunluk Hastal ığı na Kontrollü Koşullarda Etkileri

Abstract

Organik materyal Frisol F 1,13 g/kg toprak dozunda uygulanm ışt ı r. Bu materyalin tek ba şı na ve antagonistik mikroorganizmalar ile birlikte uygulanmas ı hastal ı k oluşumunu etkilememiştir. Trichoderma koningii ve T. harzianum' un ticari preparat ı Promot 1 g/kg toprak hastal ığı % 42.86 oran ı nda azaltm ış fakat bu etki istatistiksel olarak önemli bulunmam ışt ı r. Kavun tohumlar ı n ı n 2 saat süre ile Pseudomonas fluorescens ve P. putida' n ı n 109 ı r ı ld ı ktan sonra ekilmesi sonucunda solgunluk s ı ras ı yla % 54,14 ve 71,43 oran ı nda hücre/mi lik süspansiyonuna bat azalmışt ı r. İ kinci etki istatistiksel olarak önemli bulunmu ştur.

Keywords

• kavun
• fusarium solgunlu ğ u
• antagonistik mikroorganizmalar

Effects of Frisol F, Promot and Fluorescent Pseudomonads Against Fusarium Wilt of Melon Caused by Fusarium oxysporum f.sp. melonis Race 1,2 in Controlled Conditions

Abstract

The organic material, Frisol F, was incorporated to the potting soil at the rate of 1,13 g/kg soil. Application of this material alone or in combination with antagonistic microorganisms did not reduce wilt development. The commercial preparation of Trichoderma koningli and T. harzianum, Promot 1 g/kg soil reduced wilt incidence 42.86 % but this was not statistically different than the control. When melon seeds were soaked in the suspensions of Pseudomonas fluorescens and P. putida of 109cell/ml for 2 hours wilt incidence was reduced 54.14 and 71.43 % respectively, the latter being significant.

Keywords

• melon
• fusarium wilt
• antagonistic microorganisms

References

1. Anonymous, 1999. Tar ı msal Yap ı ve Üretim 1997. T.C. Başbakanl ı k Devlet istatistik Enstitüsü. 304s.
2. Bora, T., H. Özaktan, ve M. Y ı ld ı z, 1995. Tarla koşulları nda kavun ve karpuz fusarium solgunluklar ı n ı n siderofor üreten fluoresent pseudomonaslarla önlenmesi üzerinde araşt ı rmalar. VII. Türkiye Fitopatoloji Kongresi Bildirileri s: 216-219.
3. D'Ercole, N. and P. Nipoti, 1986. Biological control of fusarium and verticillium infections in tomatoes under protected cultivation, 15 (3) 55-59.
4. Erzurum, K., Y. Taner, E. Seçer, R. Yanmaz and S. Maden, 1999. Occurrence of races of Fusarium oxysporum f.sp. melonis causing wilt on melon in Central Anatolia. The Journal Turk. Phytopath, 28 (3) 87-97.
5. Filiz, N. ve S. Öz, 1996. Ege Bölgesinde Kavun Solgunluk (Fusarium oxysporum f.sp. melonis) Hastal ığı n ı n Mücadelesinde Biyoteknik Yöntemlerin Kullan ı lmas ı Üzerinde Araşt ı rmalar (Proje No: BK/04/08/207, Geli şme Raporu) Bornova Zirai Mücadele Araştı rma Enstitüsü.
6. Kim, N. K. and H. J. Jee, 1988. Influence of rhizosphere antagonists on suppression of cucumber wilt, increased cucumber growth and density fluctation of Fusarium oxysporum f.sp. cucumerinum. Korean Journal of Plant Pathology, 4 (1) 10-18.
7. Kumar, B. S. D. and H. C. Dube, 1992. Seed bacterization with a fluorescent Pseudomonas for enhanced plant growth yield and disease control. Soil Biology and Biochemistry, 24 (6) 539-542.
8. Leeman, M., R. J. Scheffer, J. A. Pelt, R. A. Van, Bakkere, and B. Shippers, 1991. Control of fusarium wilt of radish by Pseudomonas fluorescens WCS-374, in greehouse trials. Bülletin SROP, 14 (8) 34-38.

9. Moon, B. J., H. S. Chung, and C. T. Cho, 1988. Studies on antagonism of trichoderma species to Fusarium oxysporum f.sp. fragariae I. Isolation, identification and antagonistic properties of Trichoderma species. Korean of Plant Pathology, 4 (2) 111-123.
10. Ordentlich, A., Q. Mighelli, and I. Chet, 1991. Biological control activity of three Trichoderma isolates against fusarium wilts of cotton and muskmelon and lack of correlation with their lytic enzymes. Journal of Phytopathology, 133 (3) 177-186.
11. Özaktan, H. and T. Bora, 2000. Biological control of Fusarium oxysporum f.sp. melonis by the formulations of fluorescent Pseudomonads. Journal Turk. Phytopath., 29 (2-3) 133- 149.
12. Park, C. S., T. Paulitz, and R. Baker, 1988. Attributes associated biocontrol activity of fluorescent Pseudomonas. Korean Journal of Plant Pathology, 4 (3) 218-225.
13. Rumine, R. 1989. Studies of antagonistic activity of Trichoderma spp. against Fusarium oxysporum f.sp. dianthi on carnation. Annali dell Instito Sperimentale Per la Floricoltura, 20 (1) 57-67.
14. Sakhivel, N., E. Sivamani, N. Unnomalai, and S. Granamahickam, 1986. Plant growth-promoting rhizobacteria in enhancing plant growth and suppressing plant pathogens. Current Sci., 55 (1) 22-25.
15. Scarselletti, R. and J. L. Faull, 1994. In vitro activity of 6-pentyl-apyrone, a metabolite of Trichoderma harzianum in the inhibition of Rhizoctonia solani and Fusarium oxysporum f.sp. tycopetsict. Mycological Research, 98 (10) 1207-1209.
16. Sivan, A. and I. Chet, 1986a. Biological control of Fusarium spp. in cotton, wheat and muskmelon by Trichoderma harzianum. Journal of Phytopathology, 116, 39-47.
17. Sivan, A. and I. Chet, 1986b. Possible mechanisms for control of Fusarium spp. by Trichoderma harzianum. British Crop Protection Conf. Pests and Diseases, 865-872.
18. Sivan, A. and I. Chet, 1989. The possible role of competition between Trichoderma harzianum and Fusarium oxysporum on rhizosphere colonization. Phytopathology, 79 (2) 198- 203.
19. Yı ld ı z, F. 1993. Bir biyolojik ajan olan Trichoderma' n ı n biyokontroldeki yeri ve potansiyeli. Anadolu J. of AARI, 2, 112-128.
20. Yı ld ı z, M. 1977. Ege Bölgesinde Kavun Solgunluk Etmeninin Patojenitesi, Irklar ı ve Yerel Çeş itlerin Dayan ı kl ı l ı klar ı n ı n Saptanmas ı Üzerinde Araşt ı rmalar, (Doçentlik Tezi), Ege Üniv. Ziraat Fakültesi Fitopatoloji ve Zirai Botanik Kürsüsü.
21. Yücel, S., H. Pala, N. Sar ı and K. Abak, 1994. Determination of Fusarium oxysporum f.sp. melonis races in the East Mediterranean region of Türkiye and response of some melon genotypes to the diseases, 9th congress of the Mediterranean Phytopathological Union, Ku şadas ı -Türkiye, pp: 87-89.