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Genetic Diversity and Population Structure of Fusarium oxysporum f. sp. cepae, the Causal Agent of Fusarium Basal Plate Rot on Onion using RAPD Markers

Year 2010, Volume: 16 Issue: 3, 0 - , 05.09.2010
https://doi.org/10.1501/Tarimbil_0000001133

Abstract

A total of 116 single spore isolates of Fusarium oxysporum f. sp. cepae representing seven populations from Turkey and one population from Colorado, USA were subjected to molecular marker analysis using Random amplified polymorphic DNA (RAPD) technique. Using eleven polymorphic primers, 110 RAPD fragments were obtained with an average of 10 polymorphic bands per primer. Cluster analysis with UPGMA revealed five distinct lineages at arbitrary level of 65% similarity. The majority of F. oxysporum f. sp. cepae isolates from Turkey were observed to derive probably from the same clonal lineage. Genetic estimates and population differences demonstrated that the isolates from Turkey were significantly distinct from Colorado isolates and that Bursa population was the most divergent among Turkish populations. Cluster analysis of Nei's genetic distances supported populations grouping according to the geographical regions. Comparison of genetic differentiation estimates (HT: 0.140, HS: 0.124) revealed low levels of genetic differentiation among Turkish populations. Only, 11.4% of total genetic diversity (GST) attributed to differentiation among the geographical populations. Analysis of molecular variance (AMOVA) confirmed that there was low genetic differentiation among populations. The results suggest that RAPD-PCR is a useful method for analyzing genetic variation within and between populations of F. oxysporum f. sp. cepae.

Genetic Diversity and Population Structure of Fusarium oxysporum f. sp. cepae, the Causal Agent of Fusarium Basal Plate Rot on Onion using RAPD Markers

Year 2010, Volume: 16 Issue: 3, 0 - , 05.09.2010
https://doi.org/10.1501/Tarimbil_0000001133

Abstract

A total of 116 single spore isolates of Fusarium oxysporum f. sp. cepae representing seven populations from Turkey and one population from Colorado, USA were subjected to molecular marker analysis using Random amplified polymorphic DNA (RAPD) technique. Using eleven polymorphic primers, 110 RAPD fragments were obtained with an average of 10 polymorphic bands per primer. Cluster analysis with UPGMA revealed five distinct lineages at arbitrary level of 65% similarity. The majority of F. oxysporum f. sp. cepae isolates from Turkey were observed to derive probably from the same clonal lineage. Genetic estimates and population differences demonstrated that the isolates from Turkey were significantly distinct from Colorado isolates and that Bursa population was the most divergent among Turkish populations. Cluster analysis of Nei's genetic distances supported populations grouping according to the geographical regions. Comparison of genetic differentiation estimates (HT: 0.140, HS: 0.124) revealed low levels of genetic differentiation among Turkish populations. Only, 11.4% of total genetic diversity (GST) attributed to differentiation among the geographical populations. Analysis of molecular variance (AMOVA) confirmed that there was low genetic differentiation among populations. The results suggest that RAPD-PCR is a useful method for analyzing genetic variation within and between populations of F. oxysporum f. sp. cepae.

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Details

Primary Language Turkish
Journal Section Makaleler
Authors

Harun Bayraktar

Publication Date September 5, 2010
Submission Date May 24, 2010
Published in Issue Year 2010 Volume: 16 Issue: 3

Cite

APA Bayraktar, H. (2010). Genetic Diversity and Population Structure of Fusarium oxysporum f. sp. cepae, the Causal Agent of Fusarium Basal Plate Rot on Onion using RAPD Markers. Journal of Agricultural Sciences, 16(3). https://doi.org/10.1501/Tarimbil_0000001133
AMA Bayraktar H. Genetic Diversity and Population Structure of Fusarium oxysporum f. sp. cepae, the Causal Agent of Fusarium Basal Plate Rot on Onion using RAPD Markers. J Agr Sci-Tarim Bili. September 2010;16(3). doi:10.1501/Tarimbil_0000001133
Chicago Bayraktar, Harun. “Genetic Diversity and Population Structure of Fusarium Oxysporum F. Sp. Cepae, the Causal Agent of Fusarium Basal Plate Rot on Onion Using RAPD Markers”. Journal of Agricultural Sciences 16, no. 3 (September 2010). https://doi.org/10.1501/Tarimbil_0000001133.
EndNote Bayraktar H (September 1, 2010) Genetic Diversity and Population Structure of Fusarium oxysporum f. sp. cepae, the Causal Agent of Fusarium Basal Plate Rot on Onion using RAPD Markers. Journal of Agricultural Sciences 16 3
IEEE H. Bayraktar, “Genetic Diversity and Population Structure of Fusarium oxysporum f. sp. cepae, the Causal Agent of Fusarium Basal Plate Rot on Onion using RAPD Markers”, J Agr Sci-Tarim Bili, vol. 16, no. 3, 2010, doi: 10.1501/Tarimbil_0000001133.
ISNAD Bayraktar, Harun. “Genetic Diversity and Population Structure of Fusarium Oxysporum F. Sp. Cepae, the Causal Agent of Fusarium Basal Plate Rot on Onion Using RAPD Markers”. Journal of Agricultural Sciences 16/3 (September 2010). https://doi.org/10.1501/Tarimbil_0000001133.
JAMA Bayraktar H. Genetic Diversity and Population Structure of Fusarium oxysporum f. sp. cepae, the Causal Agent of Fusarium Basal Plate Rot on Onion using RAPD Markers. J Agr Sci-Tarim Bili. 2010;16. doi:10.1501/Tarimbil_0000001133.
MLA Bayraktar, Harun. “Genetic Diversity and Population Structure of Fusarium Oxysporum F. Sp. Cepae, the Causal Agent of Fusarium Basal Plate Rot on Onion Using RAPD Markers”. Journal of Agricultural Sciences, vol. 16, no. 3, 2010, doi:10.1501/Tarimbil_0000001133.
Vancouver Bayraktar H. Genetic Diversity and Population Structure of Fusarium oxysporum f. sp. cepae, the Causal Agent of Fusarium Basal Plate Rot on Onion using RAPD Markers. J Agr Sci-Tarim Bili. 2010;16(3).

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