Türkiye’nin Doğu Bölgesinden Toplanan Bazı Yerel Ekmeklik Buğday Popülasyonlarından Seçilen Saf Hatların Yüksek Molekül Ağırlıklı Glutenin Alt Birimlerinin İncelenmesi

Year 2015, Volume: 21 Issue: 3, 346 - 354, 12.08.2015

Ayşe Yıldız , Ayşe Yıldız , Kadir Akan Mevlüt Akçura

https://doi.org/10.15832/ankutbd.25025

Cited By: 1

Abstract

Bu araştırmada Türkiye’nin doğu bölgesinden toplanan bazı yerel ekmeklik buğday popülasyonlarından seçilen saf hatlardan tane verimi, verim unsurları ve bazı kalite özelliklerine göre belirlenen 41 adet genotipin, SDS-PAGE yöntemi kullanılarak Glu-1 lokusunda bulunan yüksek molekül ağırlıklı glutenin (YMAG) alt birimleri incelenmiştir. Ekmeklik buğday saf hatlarında Glu-1 lokusuna ait 9 tane allel belirlenmiştir. Glu-A1 lokusuna ait sadece null alleli (c) belirlenirken, Glu-B1 lokusuna ait 7+8 alt birimi (b) 35 (% 85.4) saf hatta 13+16 alt birimi (f) 2 (% 4.9) saf hatta, 7 (a), 14+15 (h), 17+18 (i) ve 6+8 (d) alt birimleri ise sadece 1’er (% 2.4) saf hatta gözlenmiştir. Glu-D1 lokusunda a alleli 34 (% 82.9) saf hatta, d alleli 7 (% 17.1) saf hatta belirlenmiştir. Yedi farklı YMAG alt birimleri kombinasyonlarının saptandığı çalışmada en yaygın olarak (% 78.0) gözlenen “n, 7+8 ve 2+12” kombinasyonu 32 saf hatta belirlenmiştir. Sadece 5 saf hat (% 12.2) 8 kalite skor değerini alırken, 36 (% 87.8) saf hattın skor değerinin 4-6 arasında değiştiği tespit edilmiştir.

Keywords

Türkiye yerel ekmeklik buğdayları, SDS-PAGE, Yüksek molekül ağırlıklı glutenin (YMAG)

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