The Sensitivity Against Some Acaricides and the Detoxification Enzyme Levels of Panonychus ulmi Koch Collected from Apple Orchards in Isparta

Year 2016, Volume: 22 Issue: 2, 249 - 260, 01.03.2016

Yasemin Yaman Sibel Yorulmaz Salman Recep Ay

Abstract

Susceptibility of Panonychus ulmi Koch populations collected from apple orchards in Isparta province was studied against etoxazole, spirodiclofen and hexythiazox, and their detoxification enzyme esterase, glutathione-S-transferase GST , monooxygenaz cytochrome P450 P450 and acetylcholinesterase AChE levels were determined. For this purpose, 13 P. ulmi populations were collected from Isparta province and its districts during summer periods of 2012- 2013. Acaricides were applied to mites by spray tower-leaf bioassay method. The data were analyzed by the program POLO PC and LC50 values were calculated. The resistance ratios are calculated by comparing LC50 values of field populations with LC50 values of the susceptible population. The resistance ratios in P. ulmi populations ranged from

Keywords

Panonychus ulmi, Resistance, Esterase, GST, P450, AChE

Isparta İli Elma Bahçelerinden Toplanan Panonychus ulmi Koch’nin Bazı Akarisitlere Karşı Duyarlılık ve Detoksifikasyon Enzim Düzeyleri

Abstract

Isparta ili elma bahçelerinden toplanan Panonychus ulmi Koch popülasyonlarının etoxazole, spirodiclofen, hexythiazox’a karşı duyarlılık ve detoksifikasyon esteraz, glutation-S-transferaz GST , sitokrom P450 monooxygenaz P450 ve asetilkolinesteraz AChE enzim düzeyleri belirlenmiştir. Bu amaçla Isparta ili ve ilçelerinden 2012 ve 2013 yılı üretim sezonunda toplam 13 adet P. ulmi popülasyonu toplanmıştır. Akarisiter akarlara ilaçlama kulesi-yaprak biyoassay yöntemi ile uygulanmıştır. Elde edilen veriler POLO PC programı ile analiz edilmiş ve LC50 değerleri elde edilmiştir. Tarla popülasyonlarının LC50 değerleri hassas popülasyonun LC50 değerlerine oranlanarak direnç oranları hesaplanmıştır. P. ulmi popülasyonlarının spirodiclofen’e karşı direnç düzeyleri

Keywords

Panonychus ulmi, Direnç, Esteraz, GST, P450, AChE

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