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ETLİK PİLİÇ BARSAK FLORASI ÜZERİNE YEMİN ETKİSİ

Year 1996, Volume: 02 Issue: 01, 39 - 43, 01.01.1996
https://doi.org/10.1501/Tarimbil_0000000362

Abstract

Bu çalışmada, yuınurtadan çıkıştan sonra civcivlerin do ğal barsak florasımn gelişimi ve bu gelişime sterilize edilmiş ve edilmemiş yemin etkisi araştınlmıştır. Civcivlerin barsaklarındaki doğal floramn baskm üyelerinden laktobasil, enterokok ve E.coli 'nin sayımı yapılmıştır. Sterilize edilmemiş yemle beslenen Grup II civcivlerin barsak ve körbarsaldanndaki E.coli sayısı logio , civcivlerin patojenlere duyarl ı olduklan ilk 7 gün boyunca steril yemle beslenen Grup I civcivlere göre yüksek bulunmu ştur. Grup I'de 4.günde 7.178,Grup II'de 8.058 değerleri ile en yüksek düzeye ula şmıştır. Ancak daha sonraki günlerde laktobasil ve enterokok say ılarının yükselmesiyle birlikte E.coh sayı sı Grup I'de 9.günde 4.043'e, Grup Irde 2.955'e dü şmüştür. Denenie süresince laktobasil ve enterokok sayılannda 102-108düzeyinde dalgalanmalar meydana gelmi ştir. Kullanılan yemin gramında 5.677 E. coli, 4.824 laktobasil bulunduğu, fekal streptokok ve Salmonella bulunmadığı belirlenmiştir. Salmonella tvphimurium 60-62 civcivıerde 9. günde kontrol grubuna göre 11.91 g a ğırlık kaybına neden olmuştur.

References

  • Bailey,J.S.,1993. Control of Salmonella and Campylobacter in poultry production. A summary of work at Russel Research Center. Poultry Sci., 72:1169-1173.
  • Banwart,G.J.,1975.Laboratory exercises for food microbiology.Department of Microbiology The Ohio State University,Colunıbus,Ohio,156s.
  • Barefoot, S.F, T.R. Klaenhammer, 1983. Detection and activity of Lactacin B,a bacteriocin produced by Lacidophilus. Appl. Environ. Microbiol., 45(6): 1808-1815.
  • Barrow, P.A, J. F. Tucker, 1986. lnhibition of colonization of chicken cecum with Salmonella typhimurium by pretreatment with strains of E.coli. J. Hyg. Camb., 96: 161 - 169.
  • Blankenship,L. C . , J. S . Bailey, N. A COX, N. J. Stern. R. Brewer, 0.Williams, 1993 Two-step mucosal competitive exclusion Hora traetment to diminish Salmonellae in commercial broiler chickens. Poultry Sci.,72:1667-1672.
  • Boicheva,S.V., H.Chomakov, 1988. A study of the inhibitory activity of Str.faecalis ssp. faecalis on enteropathogene E.coli. Ani.Sci., 25(4):77-80.
  • Chateau, N., I, Castellanos, A Deschamps, 1993. Distribution of pathogen inhibition in the Lactobacillus isolates of a commercial probiotic consortium. J. Appl.Bacteriol. ,74: 36- 40.
  • Chomakov,H.,S.Boicheva,1988.A study of L.reuteri and its inhibitory activity towards enteropathogene E.coliAni.Sci.,25(5):83-86.
  • Collins,C.H.,P.M.Lyne,J.M.Grange,1989.Collins and Lyne's microbiological methods. Butterworth & Co.Ltd.,London,409s.
  • Corrier.D.E. • D. J. Nisbet, A G. Holuster. C. M. Scanlan, B.M.Hargis, J.R.Deloach, 1993. De.telopment of defined cultures of indigenous ceca/ bacteria to control salmonellosis in broiler chickens.Poultry Sci.,72:1164-1168.
  • Cox, N.A, J.S.Bailey, 1993. Occurence, detection and prevention of Salmonella in poultry production.Feed Mix,1(2):44-45.
  • Çakmakçı M.L.,A.G.Karahan, 1995. Mikrobiyolojiye Giriş. Bizim Büro Bas ımevi,Ankara,227s.
  • De Man, J.C.. M.Rogosa, M.E.Sharpe, 1960.Medium for the cultivation of Lactobacilli.J.Appl. Bacteriol.,23(1):130-135.
  • Fuller,R.,1989.Probiotics in man and animals. J.Appl. Bacteriol.,66:365-378.
  • Gleeson, T. , S. S tarvic,B .Blanchfield, /989. Protection of chicks against Salmonella infection with a mixture of pure cultures of intestinal bacteria. Avian Dis.,33:636-642.
  • Harrigan, W. F. ,M.E.McCance,1966. Laboratory methods in microbiology. Academic Press, London and New York,362s.
  • Hinton,M.1986.The artificial contamination of poultry feed with Salmonella and its infectivity for young chickens.Lett.Appl. Microbiol.,3:97-99.
  • Hinton,A., D.E.Corrier, G.E.Spates, J.O.Norman, R.Ziprin,R. C.Beier, J.R.Deloach,1Q90. Biolog icat control of Salmonella ;yphimurium in young chickens.Avian Dis.,34:626-633.
  • Hinton,A, D.E.Corner, J.R.Deloach.' 1992. In vitro inhibition of Salmonella typhimurium and Escherichia coli 0157:H7 by an anaerobic gram positive coccus isolated from the cecal contents of adult chickens.J.Food Protect., 55(3):162-166.
  • Juven, B.J., N.A. Cox, J. S. Bailey, J. E.Thomson, O. W.Charles, J. V. Shutze, 1984. Recovery of Salmonella from artificially contaminated poultry feeds in non-selective and selective broth media. J. Food Prot., 47(4): 299-302.
  • Larsen,G.J.,A.Rolow,C.E.Nelson,1993.Research note: the effect of organic acids on Salmoneila contamination originating from mouse fecal pellets.Poultry Sci., 72:1797-1799.
  • McCormick,E L ,D C Savage,1983.Characterization of Lactobacillus sp.strain 100-37 from the murine gastrointestinal tract:ecology, plasmid content and antagonistic activity toward Clostridium ramosum H1. Appl. Environ. Microbiol., 46(5): 1103 -1112.
  • Savage, D.C.,1977.Microbial ecology of the gastrointestı nal tract.Ann.Rev.Microbiol.,31: 107-133.
  • Stavric, S., 1987. Microbial colonization of chicken intestine using defı ned cultures. Food Technol., 41(7):93-98 Eserin kabul tarihi: 03.07.1996

THE EFFECT OF CHICKEN FEED ON BROILER IlVTESTINAL FLORA

Year 1996, Volume: 02 Issue: 01, 39 - 43, 01.01.1996
https://doi.org/10.1501/Tarimbil_0000000362

Abstract

In this study,the main issue was to investigate the effect of steril and non steril feeding material on the establisment of the natural flora of chick intestine after hatching. Two different groups of experiments were performed. In these experiments Lactobacillus, Enterococcus and Escherichia coli were counted by dilution technique on agar plates. Non steril feeds contributed higher number of E.coli logı o to intestinal flora and cecu ın of the chicks. In general, in the intestines and cecums of chicks fed with non steril feed Group II E.coli counts were more than that of chicks fed with steril feed Group I . during 7 days that the chicks were being sensitive to the pathogens. The highest counts were recorded at 4 th day, which were 7.178 and 8.058, for Group I and Group II, respectively. E.coli counts of Group I decreased to 4.043, Group Il to 2.955 at 9th day. In addition that the number of Lactobacillus and Enterococcus number were waved between 10 2and 108 . It was found that the number of E.coli and lactobacilli were 5.677 and 4.824, respectively, and fecal streptococci and Salmonella did not exist in the feed. Artificially inoculated Salmonella tvphimurium 60-62 caused 11.91 g weight decrease compared to control chicks at 9th day.

References

  • Bailey,J.S.,1993. Control of Salmonella and Campylobacter in poultry production. A summary of work at Russel Research Center. Poultry Sci., 72:1169-1173.
  • Banwart,G.J.,1975.Laboratory exercises for food microbiology.Department of Microbiology The Ohio State University,Colunıbus,Ohio,156s.
  • Barefoot, S.F, T.R. Klaenhammer, 1983. Detection and activity of Lactacin B,a bacteriocin produced by Lacidophilus. Appl. Environ. Microbiol., 45(6): 1808-1815.
  • Barrow, P.A, J. F. Tucker, 1986. lnhibition of colonization of chicken cecum with Salmonella typhimurium by pretreatment with strains of E.coli. J. Hyg. Camb., 96: 161 - 169.
  • Blankenship,L. C . , J. S . Bailey, N. A COX, N. J. Stern. R. Brewer, 0.Williams, 1993 Two-step mucosal competitive exclusion Hora traetment to diminish Salmonellae in commercial broiler chickens. Poultry Sci.,72:1667-1672.
  • Boicheva,S.V., H.Chomakov, 1988. A study of the inhibitory activity of Str.faecalis ssp. faecalis on enteropathogene E.coli. Ani.Sci., 25(4):77-80.
  • Chateau, N., I, Castellanos, A Deschamps, 1993. Distribution of pathogen inhibition in the Lactobacillus isolates of a commercial probiotic consortium. J. Appl.Bacteriol. ,74: 36- 40.
  • Chomakov,H.,S.Boicheva,1988.A study of L.reuteri and its inhibitory activity towards enteropathogene E.coliAni.Sci.,25(5):83-86.
  • Collins,C.H.,P.M.Lyne,J.M.Grange,1989.Collins and Lyne's microbiological methods. Butterworth & Co.Ltd.,London,409s.
  • Corrier.D.E. • D. J. Nisbet, A G. Holuster. C. M. Scanlan, B.M.Hargis, J.R.Deloach, 1993. De.telopment of defined cultures of indigenous ceca/ bacteria to control salmonellosis in broiler chickens.Poultry Sci.,72:1164-1168.
  • Cox, N.A, J.S.Bailey, 1993. Occurence, detection and prevention of Salmonella in poultry production.Feed Mix,1(2):44-45.
  • Çakmakçı M.L.,A.G.Karahan, 1995. Mikrobiyolojiye Giriş. Bizim Büro Bas ımevi,Ankara,227s.
  • De Man, J.C.. M.Rogosa, M.E.Sharpe, 1960.Medium for the cultivation of Lactobacilli.J.Appl. Bacteriol.,23(1):130-135.
  • Fuller,R.,1989.Probiotics in man and animals. J.Appl. Bacteriol.,66:365-378.
  • Gleeson, T. , S. S tarvic,B .Blanchfield, /989. Protection of chicks against Salmonella infection with a mixture of pure cultures of intestinal bacteria. Avian Dis.,33:636-642.
  • Harrigan, W. F. ,M.E.McCance,1966. Laboratory methods in microbiology. Academic Press, London and New York,362s.
  • Hinton,M.1986.The artificial contamination of poultry feed with Salmonella and its infectivity for young chickens.Lett.Appl. Microbiol.,3:97-99.
  • Hinton,A., D.E.Corrier, G.E.Spates, J.O.Norman, R.Ziprin,R. C.Beier, J.R.Deloach,1Q90. Biolog icat control of Salmonella ;yphimurium in young chickens.Avian Dis.,34:626-633.
  • Hinton,A, D.E.Corner, J.R.Deloach.' 1992. In vitro inhibition of Salmonella typhimurium and Escherichia coli 0157:H7 by an anaerobic gram positive coccus isolated from the cecal contents of adult chickens.J.Food Protect., 55(3):162-166.
  • Juven, B.J., N.A. Cox, J. S. Bailey, J. E.Thomson, O. W.Charles, J. V. Shutze, 1984. Recovery of Salmonella from artificially contaminated poultry feeds in non-selective and selective broth media. J. Food Prot., 47(4): 299-302.
  • Larsen,G.J.,A.Rolow,C.E.Nelson,1993.Research note: the effect of organic acids on Salmoneila contamination originating from mouse fecal pellets.Poultry Sci., 72:1797-1799.
  • McCormick,E L ,D C Savage,1983.Characterization of Lactobacillus sp.strain 100-37 from the murine gastrointestinal tract:ecology, plasmid content and antagonistic activity toward Clostridium ramosum H1. Appl. Environ. Microbiol., 46(5): 1103 -1112.
  • Savage, D.C.,1977.Microbial ecology of the gastrointestı nal tract.Ann.Rev.Microbiol.,31: 107-133.
  • Stavric, S., 1987. Microbial colonization of chicken intestine using defı ned cultures. Food Technol., 41(7):93-98 Eserin kabul tarihi: 03.07.1996
There are 24 citations in total.

Details

Primary Language Turkish
Subjects Engineering
Journal Section Research Article
Authors

Aynur Gül Karahan This is me

M. Lütfü Çakmakçı This is me

Publication Date January 1, 1996
Submission Date January 1, 1996
Published in Issue Year 1996 Volume: 02 Issue: 01

Cite

APA Karahan, A. G., & Çakmakçı, M. L. (1996). ETLİK PİLİÇ BARSAK FLORASI ÜZERİNE YEMİN ETKİSİ. Journal of Agricultural Sciences, 02(01), 39-43. https://doi.org/10.1501/Tarimbil_0000000362

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