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Tumor Formation in Isatis constricta Davis woad Using Oncogenic A281 Strain of Agrobacterium tumefaciens

Year 2007, Volume: 13 Issue: 04, 391 - 394, 01.11.2007

Abstract

Leaf and petiole explants obtained from in vitro grown seedlings of Isatis constricta woad were treated with oncogenic A281 strain of Agrobacterium tumefaciens. Tumour formation started on explants after 15 days and continued until 4 weeks when gus test was performed. The highest gus positive results were recorded from petiole explants selected on medium containing 50 mg/l kanamycine under 16 h light photoperiod

References

  • Alibert, B., O. Lucas, V. Gall, J. Kallerhoff, B. Alibert. and V. Gall. 1999. Pectolytic enzyme treatment of sunflower prior to wounding and cocultivation with Agrobacterium tumefaciens, enhances efficieny of transient beta glucuronidase expression, Phyiologia Plantarum 106:2, 232-237.
  • Anonim http://en.wikipedia.org/wiki/woad. (12/06/2007)
  • Bhatnagar, S. and P. Khurana. 2003 Agrobacterium tumefaciens-mediated of Indian mulberry, Morus indica cv. a time-phased screening strategy Plant Cell Rep21:669–675.
  • Bidney, D., C.Scelonge, J. Martich, M. Burrus, L. Sims and G. Hoffman. 1992. Microprojectile bombardment of plant tissues increases transformation frequency by Agrobacterium tumefaciens. Plant Mol. Biol., 18: 301-313.
  • Escandon, A.S. and G. Hahne. 1991. Genotype and composition of culture medium are factors important for transformed sunflower (Helianthus annuus L) callus.Physiol. Plant., 81: 367-376.
  • Evrett, N. P., K.E.P. Robinson and D. Mascarenhas. 1987. Genetic Engineering of Sunflower (Helianthus annuus) ., Bio/Technology, 5; 1201-1204 .
  • Grayburn, W.S. and B.A. Vick. 1995. Transformation of sunflower (Helianthus annuus L.) following wounding with glass beads. Plant Cell Rep., 14: 285-289.
  • Hood, E.E. , G.L. Helmer, R.T. Fraley and M.D. Chilton. 1986. The hyper virulence of Agrobacterium tumefaciens A281 is encoded in a region of p Ti Bo542 outside of T- DNA.’’ J. Bacteriol., 168; 1291- 1301.
  • Jefferson, R.A. 1987. Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol. Biol. Rep. 5; 387-405.
  • Knittel, N., V. Gruger, G. Hahne and P. Lénéé. 1994. Transformation of sunflower (Helianthus annuus L.): A reliable protocol. Plant Cell Rep., 14: 81-86.
  • Khawar K.M., S. Gulbitti-Onarıcı, S. Çöçü, S. Erişen, C. Sancak and S. Özcan. 2004. İn vitro crown galls induced by Agrobacterium tumefaciens strain A281 (pTiBo542) in Trigonella foenum- graecum. Biologia Plantarum. 48(3): 441-444.
  • Laparra, H., M. Burrus, R. Huonold, B. Damm, A. M. Bravo- Angel, R. Bronner and G. Hahne. 1995. Expression of foreign genes in sunflower (Helianthus annuus) L.) evaluation of three gene transfer methods. Euphytica, 85: 63-74.
  • Mercuri A., L. De. Benedetti, g. Burchi and T. Schiva. (2000) Agrobacterium-mediated transformation of African violet Plant Cell, Tissue and Organ Culture60: 39–46, 2000.
  • Moyne, A.L., D. Tagu, V. Thor, C. Bergounioux, G. Freyssinet and P. Gadal. 1989.Transformation calli obtained by direct gene transfer into sunflower protoplast. Plant Cell Rep., 8: 97-100.
  • Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plantarum.15; 473-497.
  • Özcan, S. 1993. Tissue culture in pea and engineering a marker gene for specific expression in target cells for plant transformation. (Ph.D. Thesis), University of Leicester, U.K.
  • Pugliesi, C., P. Megale, F. Cecconi and S. Baroncelli. 1993. Organogenesis and embryogenesis İN Helianthus tuberosus and in the interspecific hybrid Helianthus annuus x H. tuberosus. Plant Cell Tiss. Org. Cult., 33: 187-193.
  • Schrammeijer, B., P.C. Sijmons, P.J.M. Van den Elzen and A. Hoekema. 1990. Meristem transformation of sunflower via Agrobacterium. Plant Cell rep. 9: 55- 60.
  • Tubives 2007. http:// www.tubitak.gov.tr/tubives

Onkogenik Agrobacterium tumefaciens A281 hattı ile çivit otu Isatis constricta Davis bitkisinde tümör oluşumu

Year 2007, Volume: 13 Issue: 04, 391 - 394, 01.11.2007

Abstract

Bu çalışmada, Isatis constricta çivit otu bitkisinden çimlendirme sonucu elde edilen in vitro gelişen yaprak ve yaprak sapı eksplantları Agrobacterium tumefaciens’in onkogenik A281 hattıyla inokule edilmiştir. Tümör oluşumu, eksplantlar inoküle edildikten 15 gün sonra başlamış olup, 4 hafta sonra GUS analizi yapılmıştır. En fazla tümöt oluşumu yaprak sapı eksplantında 50 mg/l kanamisin içeren aydınlık 16 saat ışık fotoperiyodunda denemesinden elde edilmiştir

References

  • Alibert, B., O. Lucas, V. Gall, J. Kallerhoff, B. Alibert. and V. Gall. 1999. Pectolytic enzyme treatment of sunflower prior to wounding and cocultivation with Agrobacterium tumefaciens, enhances efficieny of transient beta glucuronidase expression, Phyiologia Plantarum 106:2, 232-237.
  • Anonim http://en.wikipedia.org/wiki/woad. (12/06/2007)
  • Bhatnagar, S. and P. Khurana. 2003 Agrobacterium tumefaciens-mediated of Indian mulberry, Morus indica cv. a time-phased screening strategy Plant Cell Rep21:669–675.
  • Bidney, D., C.Scelonge, J. Martich, M. Burrus, L. Sims and G. Hoffman. 1992. Microprojectile bombardment of plant tissues increases transformation frequency by Agrobacterium tumefaciens. Plant Mol. Biol., 18: 301-313.
  • Escandon, A.S. and G. Hahne. 1991. Genotype and composition of culture medium are factors important for transformed sunflower (Helianthus annuus L) callus.Physiol. Plant., 81: 367-376.
  • Evrett, N. P., K.E.P. Robinson and D. Mascarenhas. 1987. Genetic Engineering of Sunflower (Helianthus annuus) ., Bio/Technology, 5; 1201-1204 .
  • Grayburn, W.S. and B.A. Vick. 1995. Transformation of sunflower (Helianthus annuus L.) following wounding with glass beads. Plant Cell Rep., 14: 285-289.
  • Hood, E.E. , G.L. Helmer, R.T. Fraley and M.D. Chilton. 1986. The hyper virulence of Agrobacterium tumefaciens A281 is encoded in a region of p Ti Bo542 outside of T- DNA.’’ J. Bacteriol., 168; 1291- 1301.
  • Jefferson, R.A. 1987. Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol. Biol. Rep. 5; 387-405.
  • Knittel, N., V. Gruger, G. Hahne and P. Lénéé. 1994. Transformation of sunflower (Helianthus annuus L.): A reliable protocol. Plant Cell Rep., 14: 81-86.
  • Khawar K.M., S. Gulbitti-Onarıcı, S. Çöçü, S. Erişen, C. Sancak and S. Özcan. 2004. İn vitro crown galls induced by Agrobacterium tumefaciens strain A281 (pTiBo542) in Trigonella foenum- graecum. Biologia Plantarum. 48(3): 441-444.
  • Laparra, H., M. Burrus, R. Huonold, B. Damm, A. M. Bravo- Angel, R. Bronner and G. Hahne. 1995. Expression of foreign genes in sunflower (Helianthus annuus) L.) evaluation of three gene transfer methods. Euphytica, 85: 63-74.
  • Mercuri A., L. De. Benedetti, g. Burchi and T. Schiva. (2000) Agrobacterium-mediated transformation of African violet Plant Cell, Tissue and Organ Culture60: 39–46, 2000.
  • Moyne, A.L., D. Tagu, V. Thor, C. Bergounioux, G. Freyssinet and P. Gadal. 1989.Transformation calli obtained by direct gene transfer into sunflower protoplast. Plant Cell Rep., 8: 97-100.
  • Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plantarum.15; 473-497.
  • Özcan, S. 1993. Tissue culture in pea and engineering a marker gene for specific expression in target cells for plant transformation. (Ph.D. Thesis), University of Leicester, U.K.
  • Pugliesi, C., P. Megale, F. Cecconi and S. Baroncelli. 1993. Organogenesis and embryogenesis İN Helianthus tuberosus and in the interspecific hybrid Helianthus annuus x H. tuberosus. Plant Cell Tiss. Org. Cult., 33: 187-193.
  • Schrammeijer, B., P.C. Sijmons, P.J.M. Van den Elzen and A. Hoekema. 1990. Meristem transformation of sunflower via Agrobacterium. Plant Cell rep. 9: 55- 60.
  • Tubives 2007. http:// www.tubitak.gov.tr/tubives
There are 19 citations in total.

Details

Primary Language Turkish
Journal Section Research Article
Authors

Çiğdem Alev Özel This is me

Publication Date November 1, 2007
Published in Issue Year 2007 Volume: 13 Issue: 04

Cite

APA Özel, Ç. A. (2007). Onkogenik Agrobacterium tumefaciens A281 hattı ile çivit otu Isatis constricta Davis bitkisinde tümör oluşumu. Journal of Agricultural Sciences, 13(04), 391-394.

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