İndirekt Enzyme-Linked Immunosorbent Assay ELISA ile Kayısı Yaprak Doku Diskierinde Plum Pox Potyvirus PPV 'nin Tanılanması

Year 2001, Volume: 07 Issue: 04, 37 - 40, 01.11.2001

İ . Özer Elibüyük

https://doi.org/10.1501/Tarimbil_0000000683

Abstract

Plum Pox Potyvirus PPV ile enfekteli kay ı sı yapraklar ı ndan bir mantar delici yard ımı yla 2, 3.5, 5 ve 6 mm çapl ı diskler kesilmiştir. Bu farkl ı çaptaki yaprak diskleri ELISA tabakalar ı ndaki kuyucuklara 1, 2, 3 ve 4'er tane olacak şekilde konulmuştur.Denemede, PPV ile enfekteli yapraklar ı n belirtili ve belirtisiz dokular ı kullan ı lmıştı r. Çal ışmada diskler için 37° C'de 4 saat ve 4° C'de bir gece olmak üzere iki inkübasyon s ı cakl ı k derecesi kullan ı lmışt ı r. Yaprak disk say ı sı ve büyüklüğ ü artt ı kça absorbans değerinin de artt ığı gözlenmiştir. Dört saat 37° C'lik bir inkübasyon peryoduna b ı rakı lan disklerin absorbans de ğerleri bir gece 4° C'de b ı rakı lanlardan biraz daha yüksek olarak bulunmu ştur. Ayr ı ca, belirtili yerlerden al ı nan disklerin belirtisizlerinkinden daha yüksek absorbans de ğeri verdiğ i görülmüştür. Bütün koşullarda enfekteli disklerin absorbans de ğerleri sağl ı kl ı ları nkinden en az iki kat daha yüksek olmu ştur

Keywords

Plum Pox Potyvirus, kay ı s ı , yaprak doku diskleri

Detection of Plum Pox Potyvirus PPV in Leaf Tissue Disks of Apricot by Indirect Enzyme-Linked Immunosorbent Assay ELISA

Abstract

The Plum Pox Potyvirus PPV infected leaf disks of 2, 3.5, 5 and 6.5 mm in diameter were cut of the leaf by cork borer. Leaf disks in different diameters were placed into the wells in the ELISA plate in increased numbers, starting with one till 4 folds. In this assay, symptomatic and asymptomatic leaves of infected trees were used. In the assay, two incubation times for disks as 37 ° C at hr and 4° C in overnight was used. It is observed that as number and size of leaf disks increase, the absorbance values were also increased. Absorbance values of incubation period of 37 ° C was greater than that of overnight. In addition, absorbance values of disks from symptomatic area was all times found a bit greater than that asymptomatic disks. Results are found to be similar both leaf disks of apricots and plums. In all conditions, absorbance values of the infected disks were at least two times greater than that of healthy ones.

Keywords

Plum Pox Potyvirus, apricot, leaf tissue disks

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