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Heritability and Genetic Parameters of Some Antioxidant Enzyme Activities in Barley (Hordeum vulgare L.) Cultivars under Salinity Stress

Year 2021, Volume 27, Issue 2, 187 - 194, 04.06.2021
https://doi.org/10.15832/ankutbd.597545

Abstract

In order to study the heritability and genetic parameters of antioxidant activity in barely (Hordeum vulgare L.) under salinity stress, a seven-parent half diallel (F1 crosses + parents) was conducted in the non-stress and salt stress (8 and 12 dS m-1) conditions in the greenhouse, during 2016-17, Ardabil, Iran. In this experiment, antioxidant enzymes ascorbate peroxidase (APX), catalase (CAT) and superoxide dismutase (SOD) were measured. The results showed that the salinity had increased the expression of all of the three enzymes and the activity of enzymes were differed under different salinity levels. The average degree of dominance was higher than unity for all cases, suggesting the control of traits by over-dominance. Under saline condition heritability in narrow sense (h2n) was found low to medium (0.11-0.41) but their broad-sense heritability (h2b) was estimated relatively high (0.74-0.90). The results suggested the lack of heterosis in control of these traits except for APX activity in 8 dS m-1 salinity. Results showed that in APX activity recessive alleles were favorable, in CAT activity, under non-stress condition, dominant alleles, and under 12 dS m-1 salinity, recessive alleles were desirable; although, such relations were not clearly revealed in SOD activity. Due to the importance of dominance, it was indicated that the evaluation of genotypes must be done at progressive breeding program. Based on general combining ability effects, it was concluded that under salinity, Rihane and Nosrat had favorable alleles for APX activity. In CAT activity, Nosrat had favorable alleles. In case of SOD, Afzal and Valfajr had favourable alleles. In spite of the importance of physiological traits as selection criteria in breeding of salinity tolerance, presence of large dominance effects should not be neglected and selection for these traits should be delayed until after some inbreeding.

References

  • Ahmad P, Jaleel C, Azooz M & Gowher N (2009). Generation of ROS and non-enzymatic antioxidants during abiotic stress in plants. Botany Research International 2: 11-20
  • Ahmed I M, Dai H, Zheng W, Cao F, Zhang G, Sun D & Wu F (2013). Genotypic differences in physiological traits in the tolerance to drought and salinity combined stress between Tibetan wild and cultivated barley. Plant Physiology and Biochemistry 63: 49- 60
  • Alscher R G, Erturk N & Heath L (2002). Role of superoxide dismutases (SOD) in controlling oxidative stress in plant. Journal of Experimental Botany 153: 1331-1341
  • Asada K, Takahashi M & Nagate M (1974). Assay and inhibitors of spinach superoxide dismutase. Agricultural and Biological Chemistry 38: 471-473
  • Ashraf M (2010). Registration of ‘S-24’spring wheat with improved salt tolerance. Journal of Plant Registeration 4: 34‒37
  • Bailly C (2004). Active oxygen species and antioxidants in seed biology. Seed Science Research 14: 93-107
  • Barnlard A, Labuschange T & Niekerk H (2001). Heritability estimates of bread wheat quality traits in the Western Cape Province of South Africa. Euphytica 127: 115-122
  • Chance B & Maehly A C (1955). Assay of catalases and peroxidases. Methods Enzymol 2: 764-775
  • Chowdhry M A, Ambreen A & Khaliq I (2002). Genetic control of some polygenic traits in vulgare species. Asian journal of Plant Science 1: 235-237
  • Flower T J (2004). Improving crop salt tolerance. Journal of Experimental Botany 55: 307-319
  • Flower T J & Yeo A R (1995). Breeding for salinity resistance in crop plants: where next? Australian Journal of Plant Physiology 22: 875-884
  • Frary A, Gol D, Keles D, Okmen B, Pinar H, Sigva H, Yemenicioglu A & Doganlar S (2010). Salt tolerance in Solanum pennellii: Antioxidant response and related QTL. BMC Plant Biology 10: 1-58
  • Garratt L C, Janagoudar B S, Lowe K C, Anthony P, Power J B & Davey M R (2002). Salinity tolerance and antioxidant status in cotton cultures. Free Radical Biology and Medicine 33: 502-511
  • Genec Y, Oldach K, Verbyla A, Lott G, Hassan M, Tester M, Wallwork H & McDonald G K (2010). Sodium exclusion QTL associated with improved seedling growth in bread wheat under salinity stress. Theoretical and Applied Genetics 121: 877-894
  • Giannopolities C N & Ries S K (1977). Superoxide dismutase. I. Occurrence in higher plants. Plant Physiology 59: 309–314
  • Griffing B (1956). A generalized treatment of the use of diallel crosses in quantiataive inheritance. Heredity 10: 31-50
  • Hayman B I (1954). The theory and analysis of diallel crosses. Genetics 39: 789-809
  • Joshi S K, Sharma S N, Singhania D L & Sain R S (2004). Combining ability in the F1 and F2 generations of diallel cross in hexaploid wheat (Triticum aestivum L. em. Thell). Hereditas 141: 115-121
  • Katerji N, Van Hoorn J W, Hamdy A, Mastrorilli M & Fares C (2006). Classification and salt tolerance analysis of barley varieties. Agricultural Water Management 85: 1-2.184-192Kim S Y, Lim J H, Park M R, Kim Y J, Park T T (2005). Enhanced antioxidant enzymes are associated with reduced hydrogen peroxide in barley roots under saline stress. Journal of Biochemistry and Molecular Biology 38 (2): 218-224
  • Kularia RK & Sharma A K (2005). Generation mean analysis for yield and its component traits in barley (Hordeum Vulgare L.). Indian Journal of Genetics and Plant Breeding 65: 129-130
  • Mahajan S & Tuteja N (2005). Cold, salinity and drought stressed: an overview. Archives of Biochemistry and Biophysics 444:139-158
  • Manchania A M, Banks S W, Gossett R, Bellaire B A, Lucas M C & Millhollon E P (1999). The influence of alpha-amanitin on NaCl induced up-regulation of antioxidant enzyme activity in cotton callus tissue. Free Radical Research 30: 429- 438
  • McDonald M B (1999). Seed deterioration Physiology, repair and assessment. Seed Science and Technology 27: 177-237
  • Munns R, Husain S, Rivelli A R, James R A, Condon A G, Lindsay M P, Lagudah E S, Schahtman D P & Hare R A (2002). Avenues for increasing salt tolerance of crops, and the role of physiologically based selection traits. Plant Soil 247: 93-105
  • Nakano Y & Asada K (1981). Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts. Plant Cell Physiology 22: 867-880
  • Naseer S H (2001). Response of barley (Hordeum vulgare L.) at various growth stages to salt stress. Journal of Biological Sciences 1(5): 326-329
  • Parida A K & Das A B (2005). Salt tolerance and salinity effects on plants. A review. Ecotoxicology and Environmental Safety 60 (11): 324-349
  • Polidoros N A & Scandalios J G (1999). Role of hydrogen peroxide and different classes of antioxidants in the regulation of catalase and glutathione S-transferase gene expression in maize (Zea mays L.). Physiologia Plantarum 106: 112-120
  • Rohman M M, Sultana R, Podder R, Tanjimul Islam A T M, Kamrul Islam M & Islam M S (2006). Nature of gene action in barley (Hordeum vulgare L.). Asian Journal of Plant Sciences 5: 170-173
  • Roychoudhury A, Basu S & Sengupta D N (2010). Amelioration of salinity stress by exogenously applied spermidine or spermine in three varieties of indica rice differing in their level of salt tolerance. Journal of Plant Physiology 168: 317-28
  • Sairam R K, Deshmukh P S & Saxena D C (1998). Role of antioxidant systems in wheat genotypes tolerance to water stress. Biologia Plantarum 41 (3): 387-394
  • Schleiff U (2008). Analysis of water supply of plants under saline soil conditions and conclusions for research on crop salt tolerance. Journal of Agronomy and Crop Science 194: 1‒8
  • Seckin B, Turkan I, Sekmen A H & Ozfidan C (2010). The role of antioxidant defense systems at differential salt tolerance of Hordeum marinum Huds. (sea barley grass) and Hordeum vulgare L. (cultivated barley). Environmental and Experimental Botany 69: 76– 85
  • Sharma R (1998). Statistical and Biometrical techniques in plant breeding. Publishers H.S. Poplai for New Age International Limited, New Delhi. pp. 178-197
  • Singh S K, Singh H C & Singh H L (2006). Inheritance of quality traits in barley (Hordeun vulgare L.). International Journal of Plant Sciences 1: 304-305
  • Singh R P & Singh S (1992). Estimation of genetic parameters through generation means analysis in bread wheat. Indian Journal of Genetics and Plant Breeding 52: 369-375
  • Singh M & Singh R K (1984). A comparison of different methods of half-diallel analysis. Theoretical and Applied Genetics 67: 323 -326
  • Tanou G, Molassiotis A & Diamantidis G (2009). Induction of reactive oxygen species and necrotic death-like destruction in strawberry leaves by salinity. Environmental and Experimental Botany 65: 270-281
  • Vashev B, Gaiser T, Ghawana T, Vries A & Stahr K (2010). Biosafor Project Deliverable 9: Cropping Potentials for Saline Areas in India, Pakistan and Bangladesh. University of Hohenheim, Hohenheim, Germany
  • Xiaoli J, Youzong H, Fanrong Z, Meixue Z & Guoping Z (2009). Genotypic difference in response of peroxidase and superoxide dismutase isozymes and activities to salt stress in barley. Acta Physiologiae Plantarum 31: 1103-1109

Year 2021, Volume 27, Issue 2, 187 - 194, 04.06.2021
https://doi.org/10.15832/ankutbd.597545

Abstract

References

  • Ahmad P, Jaleel C, Azooz M & Gowher N (2009). Generation of ROS and non-enzymatic antioxidants during abiotic stress in plants. Botany Research International 2: 11-20
  • Ahmed I M, Dai H, Zheng W, Cao F, Zhang G, Sun D & Wu F (2013). Genotypic differences in physiological traits in the tolerance to drought and salinity combined stress between Tibetan wild and cultivated barley. Plant Physiology and Biochemistry 63: 49- 60
  • Alscher R G, Erturk N & Heath L (2002). Role of superoxide dismutases (SOD) in controlling oxidative stress in plant. Journal of Experimental Botany 153: 1331-1341
  • Asada K, Takahashi M & Nagate M (1974). Assay and inhibitors of spinach superoxide dismutase. Agricultural and Biological Chemistry 38: 471-473
  • Ashraf M (2010). Registration of ‘S-24’spring wheat with improved salt tolerance. Journal of Plant Registeration 4: 34‒37
  • Bailly C (2004). Active oxygen species and antioxidants in seed biology. Seed Science Research 14: 93-107
  • Barnlard A, Labuschange T & Niekerk H (2001). Heritability estimates of bread wheat quality traits in the Western Cape Province of South Africa. Euphytica 127: 115-122
  • Chance B & Maehly A C (1955). Assay of catalases and peroxidases. Methods Enzymol 2: 764-775
  • Chowdhry M A, Ambreen A & Khaliq I (2002). Genetic control of some polygenic traits in vulgare species. Asian journal of Plant Science 1: 235-237
  • Flower T J (2004). Improving crop salt tolerance. Journal of Experimental Botany 55: 307-319
  • Flower T J & Yeo A R (1995). Breeding for salinity resistance in crop plants: where next? Australian Journal of Plant Physiology 22: 875-884
  • Frary A, Gol D, Keles D, Okmen B, Pinar H, Sigva H, Yemenicioglu A & Doganlar S (2010). Salt tolerance in Solanum pennellii: Antioxidant response and related QTL. BMC Plant Biology 10: 1-58
  • Garratt L C, Janagoudar B S, Lowe K C, Anthony P, Power J B & Davey M R (2002). Salinity tolerance and antioxidant status in cotton cultures. Free Radical Biology and Medicine 33: 502-511
  • Genec Y, Oldach K, Verbyla A, Lott G, Hassan M, Tester M, Wallwork H & McDonald G K (2010). Sodium exclusion QTL associated with improved seedling growth in bread wheat under salinity stress. Theoretical and Applied Genetics 121: 877-894
  • Giannopolities C N & Ries S K (1977). Superoxide dismutase. I. Occurrence in higher plants. Plant Physiology 59: 309–314
  • Griffing B (1956). A generalized treatment of the use of diallel crosses in quantiataive inheritance. Heredity 10: 31-50
  • Hayman B I (1954). The theory and analysis of diallel crosses. Genetics 39: 789-809
  • Joshi S K, Sharma S N, Singhania D L & Sain R S (2004). Combining ability in the F1 and F2 generations of diallel cross in hexaploid wheat (Triticum aestivum L. em. Thell). Hereditas 141: 115-121
  • Katerji N, Van Hoorn J W, Hamdy A, Mastrorilli M & Fares C (2006). Classification and salt tolerance analysis of barley varieties. Agricultural Water Management 85: 1-2.184-192Kim S Y, Lim J H, Park M R, Kim Y J, Park T T (2005). Enhanced antioxidant enzymes are associated with reduced hydrogen peroxide in barley roots under saline stress. Journal of Biochemistry and Molecular Biology 38 (2): 218-224
  • Kularia RK & Sharma A K (2005). Generation mean analysis for yield and its component traits in barley (Hordeum Vulgare L.). Indian Journal of Genetics and Plant Breeding 65: 129-130
  • Mahajan S & Tuteja N (2005). Cold, salinity and drought stressed: an overview. Archives of Biochemistry and Biophysics 444:139-158
  • Manchania A M, Banks S W, Gossett R, Bellaire B A, Lucas M C & Millhollon E P (1999). The influence of alpha-amanitin on NaCl induced up-regulation of antioxidant enzyme activity in cotton callus tissue. Free Radical Research 30: 429- 438
  • McDonald M B (1999). Seed deterioration Physiology, repair and assessment. Seed Science and Technology 27: 177-237
  • Munns R, Husain S, Rivelli A R, James R A, Condon A G, Lindsay M P, Lagudah E S, Schahtman D P & Hare R A (2002). Avenues for increasing salt tolerance of crops, and the role of physiologically based selection traits. Plant Soil 247: 93-105
  • Nakano Y & Asada K (1981). Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts. Plant Cell Physiology 22: 867-880
  • Naseer S H (2001). Response of barley (Hordeum vulgare L.) at various growth stages to salt stress. Journal of Biological Sciences 1(5): 326-329
  • Parida A K & Das A B (2005). Salt tolerance and salinity effects on plants. A review. Ecotoxicology and Environmental Safety 60 (11): 324-349
  • Polidoros N A & Scandalios J G (1999). Role of hydrogen peroxide and different classes of antioxidants in the regulation of catalase and glutathione S-transferase gene expression in maize (Zea mays L.). Physiologia Plantarum 106: 112-120
  • Rohman M M, Sultana R, Podder R, Tanjimul Islam A T M, Kamrul Islam M & Islam M S (2006). Nature of gene action in barley (Hordeum vulgare L.). Asian Journal of Plant Sciences 5: 170-173
  • Roychoudhury A, Basu S & Sengupta D N (2010). Amelioration of salinity stress by exogenously applied spermidine or spermine in three varieties of indica rice differing in their level of salt tolerance. Journal of Plant Physiology 168: 317-28
  • Sairam R K, Deshmukh P S & Saxena D C (1998). Role of antioxidant systems in wheat genotypes tolerance to water stress. Biologia Plantarum 41 (3): 387-394
  • Schleiff U (2008). Analysis of water supply of plants under saline soil conditions and conclusions for research on crop salt tolerance. Journal of Agronomy and Crop Science 194: 1‒8
  • Seckin B, Turkan I, Sekmen A H & Ozfidan C (2010). The role of antioxidant defense systems at differential salt tolerance of Hordeum marinum Huds. (sea barley grass) and Hordeum vulgare L. (cultivated barley). Environmental and Experimental Botany 69: 76– 85
  • Sharma R (1998). Statistical and Biometrical techniques in plant breeding. Publishers H.S. Poplai for New Age International Limited, New Delhi. pp. 178-197
  • Singh S K, Singh H C & Singh H L (2006). Inheritance of quality traits in barley (Hordeun vulgare L.). International Journal of Plant Sciences 1: 304-305
  • Singh R P & Singh S (1992). Estimation of genetic parameters through generation means analysis in bread wheat. Indian Journal of Genetics and Plant Breeding 52: 369-375
  • Singh M & Singh R K (1984). A comparison of different methods of half-diallel analysis. Theoretical and Applied Genetics 67: 323 -326
  • Tanou G, Molassiotis A & Diamantidis G (2009). Induction of reactive oxygen species and necrotic death-like destruction in strawberry leaves by salinity. Environmental and Experimental Botany 65: 270-281
  • Vashev B, Gaiser T, Ghawana T, Vries A & Stahr K (2010). Biosafor Project Deliverable 9: Cropping Potentials for Saline Areas in India, Pakistan and Bangladesh. University of Hohenheim, Hohenheim, Germany
  • Xiaoli J, Youzong H, Fanrong Z, Meixue Z & Guoping Z (2009). Genotypic difference in response of peroxidase and superoxide dismutase isozymes and activities to salt stress in barley. Acta Physiologiae Plantarum 31: 1103-1109

Details

Primary Language English
Subjects Engineering
Journal Section Makaleler
Authors

Varahram RASHİDİ (Primary Author)
Department of Agronomy and Plant Breeding, Islamic Azad University, Tabriz Branch
0000-0001-6541-2709
Iran


Jamal RAHİMİ DARABAD This is me
Department of Agronomy and Plant Breeding, Islamic Azad University, Tabriz Branch
0000-0003-1905-7405
Iran


Hossein SHAHBAZİ This is me
Department of Agronomy and Plant Breeding, Islamic Azad University, Ardabil branch
0000-0002-3227-5014
Iran


Mohammad MOGHADDAM VAHED This is me
Department of Plant Biotechnology and Breeding, Tabriz University
0000-0003-0536-9002
Iran


Ebrahim KHALİLVAND This is me
Department of Agronomy and Plant Breeding, Islamic Azad University, Tabriz Branch
0000-0003-2553-3500
Iran

Supporting Institution Islamic Azad University, Tabriz Branch, Tabriz, Iran
Project Number PhD thesis of Jamal Rahimi
Thanks Thanks to Islamic Azad University, Tabriz Branch
Publication Date June 4, 2021
Application Date July 27, 2019
Acceptance Date January 15, 2020
Published in Issue Year 2021, Volume 27, Issue 2

Cite

Bibtex @research article { ankutbd597545, journal = {Journal of Agricultural Sciences}, issn = {}, eissn = {2148-9297}, address = {}, publisher = {Ankara University}, year = {2021}, volume = {27}, pages = {187 - 194}, doi = {10.15832/ankutbd.597545}, title = {Heritability and Genetic Parameters of Some Antioxidant Enzyme Activities in Barley (Hordeum vulgare L.) Cultivars under Salinity Stress}, key = {cite}, author = {Rashidi, Varahram and Rahimi Darabad, Jamal and Shahbazi, Hossein and Moghaddam Vahed, Mohammad and Khalilvand, Ebrahim} }
APA Rashidi, V. , Rahimi Darabad, J. , Shahbazi, H. , Moghaddam Vahed, M. & Khalilvand, E. (2021). Heritability and Genetic Parameters of Some Antioxidant Enzyme Activities in Barley (Hordeum vulgare L.) Cultivars under Salinity Stress . Journal of Agricultural Sciences , 27 (2) , 187-194 . DOI: 10.15832/ankutbd.597545
MLA Rashidi, V. , Rahimi Darabad, J. , Shahbazi, H. , Moghaddam Vahed, M. , Khalilvand, E. "Heritability and Genetic Parameters of Some Antioxidant Enzyme Activities in Barley (Hordeum vulgare L.) Cultivars under Salinity Stress" . Journal of Agricultural Sciences 27 (2021 ): 187-194 <https://dergipark.org.tr/en/pub/ankutbd/issue/62584/597545>
Chicago Rashidi, V. , Rahimi Darabad, J. , Shahbazi, H. , Moghaddam Vahed, M. , Khalilvand, E. "Heritability and Genetic Parameters of Some Antioxidant Enzyme Activities in Barley (Hordeum vulgare L.) Cultivars under Salinity Stress". Journal of Agricultural Sciences 27 (2021 ): 187-194
RIS TY - JOUR T1 - Heritability and Genetic Parameters of Some Antioxidant Enzyme Activities in Barley (Hordeum vulgare L.) Cultivars under Salinity Stress AU - Varahram Rashidi , Jamal Rahimi Darabad , Hossein Shahbazi , Mohammad Moghaddam Vahed , Ebrahim Khalilvand Y1 - 2021 PY - 2021 N1 - doi: 10.15832/ankutbd.597545 DO - 10.15832/ankutbd.597545 T2 - Journal of Agricultural Sciences JF - Journal JO - JOR SP - 187 EP - 194 VL - 27 IS - 2 SN - -2148-9297 M3 - doi: 10.15832/ankutbd.597545 UR - https://doi.org/10.15832/ankutbd.597545 Y2 - 2020 ER -
EndNote %0 Journal of Agricultural Sciences Heritability and Genetic Parameters of Some Antioxidant Enzyme Activities in Barley (Hordeum vulgare L.) Cultivars under Salinity Stress %A Varahram Rashidi , Jamal Rahimi Darabad , Hossein Shahbazi , Mohammad Moghaddam Vahed , Ebrahim Khalilvand %T Heritability and Genetic Parameters of Some Antioxidant Enzyme Activities in Barley (Hordeum vulgare L.) Cultivars under Salinity Stress %D 2021 %J Journal of Agricultural Sciences %P -2148-9297 %V 27 %N 2 %R doi: 10.15832/ankutbd.597545 %U 10.15832/ankutbd.597545
ISNAD Rashidi, Varahram , Rahimi Darabad, Jamal , Shahbazi, Hossein , Moghaddam Vahed, Mohammad , Khalilvand, Ebrahim . "Heritability and Genetic Parameters of Some Antioxidant Enzyme Activities in Barley (Hordeum vulgare L.) Cultivars under Salinity Stress". Journal of Agricultural Sciences 27 / 2 (June 2021): 187-194 . https://doi.org/10.15832/ankutbd.597545
AMA Rashidi V. , Rahimi Darabad J. , Shahbazi H. , Moghaddam Vahed M. , Khalilvand E. Heritability and Genetic Parameters of Some Antioxidant Enzyme Activities in Barley (Hordeum vulgare L.) Cultivars under Salinity Stress. J Agr Sci-Tarim Bili. 2021; 27(2): 187-194.
Vancouver Rashidi V. , Rahimi Darabad J. , Shahbazi H. , Moghaddam Vahed M. , Khalilvand E. Heritability and Genetic Parameters of Some Antioxidant Enzyme Activities in Barley (Hordeum vulgare L.) Cultivars under Salinity Stress. Journal of Agricultural Sciences. 2021; 27(2): 187-194.
IEEE V. Rashidi , J. Rahimi Darabad , H. Shahbazi , M. Moghaddam Vahed and E. Khalilvand , "Heritability and Genetic Parameters of Some Antioxidant Enzyme Activities in Barley (Hordeum vulgare L.) Cultivars under Salinity Stress", Journal of Agricultural Sciences, vol. 27, no. 2, pp. 187-194, Jun. 2021, doi:10.15832/ankutbd.597545