Searching of Pregnancy Rate in Repeat Breeder Cows by Embryo Transfer Practices

Year 2021, Volume: 27 Issue: 4, 436 - 440, 04.12.2021

Erkan Say , Hakan Sağırkaya

https://doi.org/10.15832/ankutbd.719266

Abstract

The aim of this study is to compare the pregnancy rates by applying embryo transfer to the cows which are not pregnant and should be removed from herd as repeat breeder. In this study, 87 randomly
selected Holstein cows utilized. The repeat breeder cows (n=45) were selected from at least one giving birth, having regular sexual cycle, missing clinical worsening into genital organ and not displaying an
abnormal discharge. On the other hand, it was selected from nonpregnant cows which inseminated artificially at least 3 times or more. Besides, cows that used as the control group (n=42) were selected from
the cows without any artificial insemination postnatally. The PGF2α application was performed to all recipient cows which are considered to benefit from as a recipient in control and testing groups just 24 days
before the flushing day. After this application, the cows showing estrous symptoms were recorded and determined as candidate recipients. The pregnancy rates were 35.6% and 50% for testing and control groups, respectively and the difference between the groups was significant (P<0,05). As a result, even this difference between the groups it has been concluded that embryo transfer can be used to conceive especially for high-yielding cows as a treatment method for repeat breeder cows. Thereby, embryo transfer from the cows with high superior characteristics to the high milk yielding cows which have infertility
problems can economically be beneficial by utilizing the high milk yield in the later lactation without any replacement cost.

Keywords

Embryo Transfer, Holstein, Infertility, Repeat Breeder

References

• Akyol N (2001): Sığır embriyo transferinde hormon kulanımı. Lalahan Hayvancılık Araştırma Enstitüsü Dergisi, 44 (1), 95-104. Alaçam E (2010): İnekte İnfertilite Sorunu. Editör: Alaçam E, Evcil Hayvanlarda Doğum ve İnfertilite.7. Baskı, Medisan Yayınları, Ankara, 267-290.
• Aslan S, Wesenauer G (1999): İneklerde gebelik, embriyonik-fötal ölümler, ovaryum fonksiyonları ve uterus çapının ultrasonografi ile saptanması. Turkish Journal of Veterinary and Animal Sciences, 23 (3), 623-631.
• Bilby TR (2010): Improving fertility in the repeat breeder. http://articles.extension.org/pages/28606/improving-fertility-in-the-repeat-breeder#Embryo_Transfer. (05.05.2017).
• Bülbül B, Dursun Ş (2005): İneklerde süperovulasyon cevabına etki eden faktörler. Hayvancılık Araştırma Dergisi, 15 (1), 16-25.
• Dochi O, Takahashi K, Hirai T, et al. (2008): The use of embryo transfer to produce pregnancies in repeat-breeding dairy cattle. Theriogenology, 69, 124–128.
• Ergene O (2012): Progesteron concentrations and pregnancy rates of repeat breeder cows following postinsemination PRID and GnRH treatments. Turkish Journal of Veterinary and Animal Sciences, 36 (3), 283-288.
• Ergene O (2009): Repeat breeder ineklerde tohumlamayı izleyen farklı günlerde PRID ve GnRH ile sağaltım girişimleri(Doktora Tezi). Ankara Üniversitesi Sağlık Bilimleri Enstitüsü Doğum ve Jinekoloji Ana Bilim Dalı, Ankara.
• Kanagawa H, Shimohira I, Saitoh N (1995): Manual of Bovine Embryo Transfer. National Livestock Breeding Center MAFF, JICA-Japan, 1-34.
• Karaşahin T, Dursun Ş, Kızıl SH (2016): Effect of embryo transfer to conception rates in repeat breeding cattles. 1st International Congress on Advances in Veterinary Sciences and Technics (ICAVST), Sarajeva, 30.
• Kaymaz M (2012): Yardımcı Üreme Teknikleri. Editör: Semacan A, Kaymaz M Fındık M et al, Çiftlik Hayvanlarında Doğum Jinekololji. 2. Baskı, Medipres Matbaacılık Yayıncılık Ltd. Şti., Malatya, 695-811.
• Kızıl SH, Akyol N, Karaşahin T, et al. (2011): Etilen glikol ile direkt transfer metoduna göre dondurulan in vivo sığır embriyolarının transferi. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 17 (5), 721-724.
• Kim SY, Jeong JK, Lee SC, et al. (2017): Risk factors for late embryonic mortality in dairy cows. Journal of Veterinary Clinics, 34 (2), 82-86.
• Pabuçcuoğlu S (2013): İneklerde Üremenin Kontrolü ve Embriyo Transferi. aves.istanbul.edu.tr/ImageOfByte.aspx?Resim=8&SSNO=7&USER=1653. (04.05.2017).
• Perez-Marin CC, Moreno LM, Calero GV (2012): Clinical Approach to the Repeat Breeder Cow Syndrome. Editor: Perez-Marin CC, A Bird's-Eye View of Veterinary Medicine. University of Cordoba, Spain, 337-362.
• Riha J, Machatkova M, Pavlok A (2002): Viability of fresh and frozen transferred IVP bovine embryos. Czech Journal Animal Science, 47 (7), 261–267.
• Rodrigues CA, Teixeira AA, Ferreira RM, et al. (2010): Effect of fixed-time embryo transfer on reproductive efficiency in high-producing repeat-breeder holstein cows. Animal Reproduction Science, 118, 110–117.
• Rutledge JJ (2001): Use of embryo transfer and ivf to bypass effects of heat stress. Theriogenology, 55, 105-111.
• Santos JEP, Cerri RLA, Sartori R (2008): Nutritional management of the donor cow. Theriogenology, 69, 88–97.
• Santos JEP, Thatcher WW, Chebel RC, et al. (2004): The effect of embryonic death rates in cattle on the efficacy of estrus synchronization programs. Animal Reproduction Science, 82-83, 513-535.
• Seidel GE, Seidel SM (1991): Training Manual for Embryo transfer in Cattle, http://www.fao.org/DOCREP/004/T0117E/T0117E00.htm,(26.05.2017). (26.05.2017).
• Selvaraju M, Veerapandian C (2010): Effect of PGF2α on oestrus and fertility rate in repeat breeder cows treated with norgestomet-oestradiol. Veterinary World, 3 (10), 466-468. Silke V, Diskin MG, Kenny DA, et al. (2002): Extent, pattern and factors associated with late embryonic loss in dairy cows. Animal Reproduction Science, 71, 1–12.
• Silva JCC, Alvarez RH, Zanenga CA, et al. (2009): Factors affecting embryo production in superovulated nelore cattle. Animal Reproduction, 6(3), 440-445.
• Son DS, Choe CY, Cho SR, et al. (2007): A CIDR-based timed embryo transfer protocol increases the pregnancy rate of lactating repeat breeder dairy cows. Journal of Reproduction and Develeopment, 53, 1313-1318.
• SPSS for Windows. Base SystemUser’s Guide, Release11.5, PSS Inc. Chicago, USA, 1999. Stradaioli G, Biancucci A, Sbaragli T, et al. (2015): Preliminary results from a field study on the use of et to improve reproductive performance in repeat breeder cows. LXVIII Convegno Sisvet, XI Convegno Aipvet e XII Convegno Sira, Perugia, 318.
• Tekeli T (2010): Embriyo Nakli. Editör: Alaçam E, Evcil Hay¬vanlarda Doğum ve İnfertilite.7. Baskı, Medisan Yayınları, Ankara, 81-97.