Isolation and Characterization of Rhizospheric Bacteria from Vuralia turcica Rhizospheric Soil

Year 2023, Volume: 29 Issue: 1, 308 - 316, 31.01.2023

Cem Çiftçi , Dilek Tekdal , Burçin Çıngay , Selim Çetiner

https://doi.org/10.15832/ankutbd.923451

Abstract

Plant growth-promoting rhizobacteria are highly active in soil ecosystems for legumes due to their biotic activities. Vuralia turcica (Kit Tan, Vural & Kucukoduk) Uysal & Ertugrul is a Turkish endemic legume plant with potential value as ornamental and food crops. However, reports of plant growth-promoting rhizobacteria in V. turcica rhizosphere are lacking in the literature. The purpose of this study was the isolation and characterization of endophytic bacteria from V. turcica rhizospheric soil. Ten bacterial strains were isolated and identified by comparing the 16S rRNA and 16S-23S rRNA ITS region. 4 isolates belonged to Bacillus megaterium, 3 strains belonged to Stenotrophomonas rhizophila, 1 strain belonged to Rhodococcus erythropolis, 1 strain belonged to Xanthomonas albilineans. The remaining 1 strain belonged to Lysobacter enzymogenes, respectively.

Keywords

Growth -promoting, 16S rRNA, 16S-23S rRNA ITS region, Molecular characterization

Thanks

The authors are grateful to the Nezahat Gökyiğit Botanical Garden for providing the materials in the study and thank Dr. Mustafa Atilla Yazıcı and Yusuf Tutuş for their technical assistance in the mineral element analysis.

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