Changes in the Major Antioxidant Compounds of Red Cabbage Under Water Stress Applied at Different Vegetative Growth Periods
Year 2025,
Volume: 31 Issue: 1, 242 - 251, 14.01.2025
Okan Erken
,
Murat Yıldırım
,
Bayram Kızılkaya
,
Umut Mucan
Abstract
Effective and efficient use of water resources has become an important issue in recent studies, where the impacts of climate change has become more apparent and alternative solutions are discussed. There, however, are limited studies that look at the impacts of water stress at different vegetative periods. For this reason, in this study, different levels of water treatment (0%, 30%, 70%) were applied to red cabbage at two stages of development (early and late vegetative) in a two-year field study. The effect of water stress on the major antioxidant compounds, as well as on yield and some morphological parameters were investigated.
According to the findings, the least yield loss (22%) occurred in the early vegetative period of the second-year trial where 70% irrigation water was applied, while the highest yield loss (56%) was obtained during the early vegetative period of the first-year trial where no irrigation was applied. Biochemical analyses revealed that the highest accumulation of flavonoids, 0.83 mg g-1, and anthocyanins, 1.51 mg g-1, occurred in the early vegetative period with the trial that received no irrigation treatment. The phenolic compound content was determined as 1.62 mg g-1, and the antioxidant capacity was found to be 1.93 mg g-1 during the late vegetative period in the trials without irrigation treatment. These findings suggest that in regions with limited water resources, water conservation can be practiced during different vegetative periods in order to get higher biochemical benefits with a lower yield loss when cultivating red cabbage.
Project Number
Project Number: FBA-2021-3687
Thanks
This work was supported by Çanakkale Onsekiz Mart University, the Scientific Research Coordination Unit.
References
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- Kishor N, Khanna M, Rajanna G A, Singh M, Singh A, Banerjee T, Patanjali N, Singh S, Parihar C M, Prasad S, Manu S M, Kiruthiga B & Arockia A (2023). Red cabbage (Brassica oleracea) response to hydrogels under drip irrigation and fertigation regimes. The Indian Journal of Agricultural Sciences, 93 (5): 529–533. https://doi.org/10.56093/ijas.v93i5.132723
- Kumar P S, Singh Y, Nangare D D, Bhagat K, Kumar M, Taware P B & Minhas P S (2015). Influence of growth stage specific water stress on the yield, physio-chemical quality and functional characteristics of tomato grown in shallow basaltic soils. Scientia Horticulturae, 197: 261-271. http://dx.doi.org/10.1016/j.scienta.2015.09.054
- Kusvuran S & Abak K (2012). Kavun Genotiplerinin Kuraklık Stresine Tepkileri. ÇU Uni Fen ve Müh Bil Der 28(5): 78-87 (In Turkish).
- Laoué J, Fernandez C & Ormeño E (2022). Plant flavonoids in Mediterranean species: A focus on flavonols as protective metabolites under climate stress. Plants, 11 (2): 172. https://doi.org/10.3390/plants11020172
- Lin J Y, Li C Y & Hwang I F (2008). Characterization of the pigment components in red cabbage (Brassica oleracea L. var.) juice and their anti-inflammatory effects on LPS-stimulated murine splenocytes. Food Chemistry, 109 (4): 771-781. https://doi.org/10.1016/j.foodchem.2008.01.039
- Lobos T E, Retamales J B, Ortega-Farías S, Hanson E J, López-Olivari R & Mora M L (2017). Regulated deficit irrigation effects on physiological parameters, yield, fruit quality and antioxidants of Vaccinium corymbosum plants cv. Brigitta. Irrigation Science, 36: 49-60. https://doi.org/10.1007/s00271-017-0564-6
- Magalhaes L M, Santos F, Segundo M A, Reis S & Lima J L (2010). Rapid microplate high-throughput methodology for assessment of Folin-Ciocalteu reducing capacity. Talanta 83(2): 441-447
- Majkowska-Gadomska J & Wierzbicka B (2008). Content of Basic Nutrients and Minerals in Heads of Selected Varieties of Red Cabbage (Brassica oleracea var. capitata f. rubra). Polish Journal of Environmental Studies 17(2): 295-298. https://doi.org/10.1016/j.scienta.2020.109561
- Mazza G & Miniati E (1993). Anthocyanins in Fruits, Vegetables and Grains. CRC Press, Boca Raton, FL.
MGM.0 (2023). Temperature, Precipitation and Evaporation Data. The Turkish State Meteorological Service (in Turkish: Meteoroloji Genel Müdürlüğü). Received February 23, 2023.
- Podsedek A (2007). Natural antioxidants and antioxidant capacity of Brassica vegetables: A review. LWT- Food Science and Technology, 40: 1-11. https://doi.org/10.1016/j.lwt.2005.07.023
- Reyes L F, Villarreal J E & Cisneros-Zevallos L (2017). The increase in antioxidant capacity after wounding depends on the type of fruit or vegetable tissue. Food Chemistry 101(3): 1254-1262. https://doi.org/10.1016/j.foodchem.2006.03.032
- Saha C, Bhattacharya P, Sengupta S, Dasgupta S, Patra S K, Bhattacharyya K & Dey P (2021). Response of cabbage to soil test-based fertilization coupled with different levels of drip irrigation in an inceptisol. Irrigation Science 1-15. https://doi.org/10.1007/s00271-021-00761-z
- Semiz G D, Şentürk C, Yildirim A C & Torun E (2023). Modelling Yield Response and Water Use to Salinity and Water Relations of Six Pepper Varieties. Journal of Agricultural Sciences (Tarim Bilimleri Dergisi) 29(1):188-199. DOI: 10.15832/ankutbd.1017255
- Shawon R A, Kang B S, Lee S G, Kim S K, Lee H J, Katrich E, Gorinstein S & Ku Y G (2020). Influence of drought stress on bioactive compounds, antioxidant enzymes and glucosinolate contents of Chinese cabbage (Brassica rapa). Food Chemistry 308: 1–9. https://doi.org/10.1016/j.foodchem.2019.125657.
- Shinde M G, Pawar D D, Kale K D & Dingre S K (2020). Performance of cabbage at different irrigation levels under drip and micro sprinkler irrigation systems. Irrigation Drainage Published online in Wiley Online Library Cited 02 June 2021. https://onlinelibrary.wiley.com/doi/pdf/10.1002/ird.2557.
- Siro I, Kápolna E, Kápolna B & Lugasi A (2008). Functional food. Product development, marketing and consumer acceptance—A review. Appetite 51(3): 456-467
- Šola I, Stić P & Rusak G (2021). Effect of flooding and drought on the content of phenolics, sugars, photosynthetic pigments and vitamin C and antioxidant potential of young Chinese cabbage. European Food Research and Technology 247: 1913-1920. https://doi.org/10.1007/s00217-021-03759-1
- Şahin U, Ekinci M, Ors S, Turan M, Yıldız S & Yıldırım E (2018). Effects of individual and combined effects of salinity and drought on physiological, nutritional and biochemical properties of cabbage (Brassica oleracea var. capitata). Scientia Horticulturae 240: 196–204. https://doi.org/10.1016/j.scienta.2018.06.016
- Valifard M, Mohsenzadeh S & Kholdebarin B (2017). Salinity effects on phenolic content and antioxidant activity of Salvia macrosiphon. Iranian Journal of Science and Technology, Transaction A 41: 295–300. https://doi.org/10.1007/s40995-016-0022-y.
- Volden J, Borge G I A, Bengtsson G B, Hansen M, Thygesen I E & Wicklund T (2008). Effect of thermal treatment on glucosinolates and antioxidant-related parameters in red cabbage (Brassica oleracea L. ssp. capitata f. rubra). Food Chemistry 109: 595-605. https://doi.org/10.1016/j.foodchem.2008.01.010
- Wallace J S (2000). Increasing agricultural water use efficiency to meet future food production. Agriculture, Ecosystems & Environment, 82(1-3): 105-119. https://doi.org/10.1016/S0167-8809(00)00220-6
- Wiczkowski W, Szawara-Nowak D & Topolska J (2013). Red cabbage anthocyanins: profile, isolation, identification, and antioxidant activity. Food Research International 51: 303-309. https://doi.org/10.1016/j.foodres.2012.12.015
- Zhang X, Lu G, Long W, Zou X, Li F & Nishio T (2014). Recent progress in drought and salt tolerance studies in Brassica crops. Breeding Science 64: 60-73. https://doi.org/10.1270/jsbbs.64.60
Year 2025,
Volume: 31 Issue: 1, 242 - 251, 14.01.2025
Okan Erken
,
Murat Yıldırım
,
Bayram Kızılkaya
,
Umut Mucan
Project Number
Project Number: FBA-2021-3687
References
- Ahmadiani N, Robbins R J, Collins T M & Giusti M M (2014). Anthocyanins contents, profiles, and color characteristics of red cabbage extracts from different cultivars and maturity stages. Journal of Agricultural and Food Chemistry 62: 7524–7531. https://doi.org/10.1021/jf501991q.
- Ak I & Türker G (2018). Antioxidant activity of five seaweed extracts. New knowledge Journal of science 7: 149–155
- Apak R, Güçlü K, Ozyürek M & Karademir S E (2004). Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. Journal of Agricultural and Food Chemistry, 52: 7970–7981. https://doi.org/10.1021/jf048741x.
- Beacham A M, Hand P, Pink D A & Monaghan J M (2017). Analysis of Brassica oleracea early stage abiotic stress responses reveals tolerance in multiple crop types and for multiple sources of stress. Journal of the Science of Food and Agriculture, 97: 5271–5277. https://doi.org/10.1002/jsfa.8411.
- Benvenuti S, Pellati F, Melegari M A & Bertelli D (2004). Polyphenols, anthocyanins, ascorbic acid, and radical scavenging activity of Rubus, Ribes, and Aronia. Journal of Food Science, 69: 164–169. https://doi.org/10.1111/j.1365-2621.2004.tb13352.x.
- Brand-Williams W, Cuvelier M E & Berset C (1995). Use of a free radical method to evaluate antioxidant activity. LWT - Food Science and Technology, 28: 25–30. https://doi.org/10.1016/S0023-6438(95)80008-5
- Bute A, Iosob G A, Antal-Tremurici A, Brezeanu C, Brezeanu P M, Cristea T O & Ambăruş S (2021). The Most Suitable Irrigation Methods in Cabbage Crops (Brassica Oleracea L. var. Capitata): A Review. Sci Papers Series B. Horticulture, 65(1)
- Chu Y F, Sun J W & Liu R H (2002). Antioxidant and antiproliferative activities of common vegetables. Journal of Agricultural and Food Chemistry, 50: 6910-6916. https://doi.org/10.1021/jf020665f
- Cohen J, Kristal R & Stanford J (2000). Fruit and vegetable intakes and prostate cancer. Journal of the National Cancer Institute 9: 61-68. https://doi.org/10.1093/jnci/92.1.61
- Dhungel R, Anderson R, French G A, Skaggs N T, Saber H, Sanchez M C A & Scudiero E (2023). Early season irrigation detection and evapotranspiration modeling of winter vegetables based on Planet satellite using water and energy balance algorithm in lower Colorado basin. Irrigation Science, 1-13. https://doi.org/10.1007/s00271-023-00874-7
- Erken O (2022). Some bioactive metabolites’ response to long-term water stress in red cabbage. Scientia Horticulturae 293: 110731. https://doi.org/10.1016/j.scienta.2021.110731
- Giusti M M, Rodriguez-Saona L E, Baggett J R, Reed L, Durst R W & Wrolstad R E (1998). Anthocyanin pigment composition of red radish cultivars as potential food colorants. Journal of Food Science, 63: 219–224. https://doi.org/10.1111/j.1365-2621.1998.tb15713.x
- Haghighi M, Saadat S & Abbey L (2020). Effect of exogenous amino acids application on growth and nutritional value of cabbage under drought stress. Scientia Horticulturae, 272: 109561 p.1-7. https://doi.org/10.1016/j.scienta.2020.109561
- Hajiboland R. & Amirazad H (2010). Drought tolerance in Zn-deficient red cabbage (Brassica oleracea L. var. capitata f. rubra) plants. Horticultural Science, 37(3): 88-98. https://doi.org/10.17221/64/2009-HORTSCI
- Hegazi A M & El-Shraiy A M (2017). Stimulation of photosynthetic pigments, anthocyanin, antioxidant enzymes in salt stressed red cabbage plants by ascorbic acid and potassium silicate. Middle East Journal of Agriculture Research, 6(2): 553-568
- Igarashi K, Kimura Y & Takenaka A (2000). Preventive effect of dietary cabbage acylated anthocyanins on paraquat induced oxidative stress in rats. Bioscience, Biotechnology, and Biochemistry, 64: 1600-7. https://doi.org/10.1271/bbb.64.1600
- Jafari S, Garmdareh S H E & Azadegan B (2019). Effects of drought stress on morphological, physiological, and biochemical characteristics of stock plant (Matthiola incana L.). Scientia Horticulturae, 253: 128–133. https://doi.org/10.1016/j.scienta.2019.04.033
- Janabi A H W, Kamboh A A, Saeed M, Xiaoyu L, Bibi J, Majeed F, Naveed M, Mughal M J, Korejo R A, Kamboh R, Alagawany M & Lv H (2020). Flavonoid-rich foods (FRF): A promising nutraceutical approach against lifespan-shortening diseases. Iranian Journal of Basic Medical Sciences, 23(2): 140. doi: 10.22038/IJBMS.2019.35125.8353
- Kishor N, Khanna M, Rajanna G A, Singh M, Singh A, Banerjee T, Patanjali N, Singh S, Parihar C M, Prasad S, Manu S M, Kiruthiga B & Arockia A (2023). Red cabbage (Brassica oleracea) response to hydrogels under drip irrigation and fertigation regimes. The Indian Journal of Agricultural Sciences, 93 (5): 529–533. https://doi.org/10.56093/ijas.v93i5.132723
- Kumar P S, Singh Y, Nangare D D, Bhagat K, Kumar M, Taware P B & Minhas P S (2015). Influence of growth stage specific water stress on the yield, physio-chemical quality and functional characteristics of tomato grown in shallow basaltic soils. Scientia Horticulturae, 197: 261-271. http://dx.doi.org/10.1016/j.scienta.2015.09.054
- Kusvuran S & Abak K (2012). Kavun Genotiplerinin Kuraklık Stresine Tepkileri. ÇU Uni Fen ve Müh Bil Der 28(5): 78-87 (In Turkish).
- Laoué J, Fernandez C & Ormeño E (2022). Plant flavonoids in Mediterranean species: A focus on flavonols as protective metabolites under climate stress. Plants, 11 (2): 172. https://doi.org/10.3390/plants11020172
- Lin J Y, Li C Y & Hwang I F (2008). Characterization of the pigment components in red cabbage (Brassica oleracea L. var.) juice and their anti-inflammatory effects on LPS-stimulated murine splenocytes. Food Chemistry, 109 (4): 771-781. https://doi.org/10.1016/j.foodchem.2008.01.039
- Lobos T E, Retamales J B, Ortega-Farías S, Hanson E J, López-Olivari R & Mora M L (2017). Regulated deficit irrigation effects on physiological parameters, yield, fruit quality and antioxidants of Vaccinium corymbosum plants cv. Brigitta. Irrigation Science, 36: 49-60. https://doi.org/10.1007/s00271-017-0564-6
- Magalhaes L M, Santos F, Segundo M A, Reis S & Lima J L (2010). Rapid microplate high-throughput methodology for assessment of Folin-Ciocalteu reducing capacity. Talanta 83(2): 441-447
- Majkowska-Gadomska J & Wierzbicka B (2008). Content of Basic Nutrients and Minerals in Heads of Selected Varieties of Red Cabbage (Brassica oleracea var. capitata f. rubra). Polish Journal of Environmental Studies 17(2): 295-298. https://doi.org/10.1016/j.scienta.2020.109561
- Mazza G & Miniati E (1993). Anthocyanins in Fruits, Vegetables and Grains. CRC Press, Boca Raton, FL.
MGM.0 (2023). Temperature, Precipitation and Evaporation Data. The Turkish State Meteorological Service (in Turkish: Meteoroloji Genel Müdürlüğü). Received February 23, 2023.
- Podsedek A (2007). Natural antioxidants and antioxidant capacity of Brassica vegetables: A review. LWT- Food Science and Technology, 40: 1-11. https://doi.org/10.1016/j.lwt.2005.07.023
- Reyes L F, Villarreal J E & Cisneros-Zevallos L (2017). The increase in antioxidant capacity after wounding depends on the type of fruit or vegetable tissue. Food Chemistry 101(3): 1254-1262. https://doi.org/10.1016/j.foodchem.2006.03.032
- Saha C, Bhattacharya P, Sengupta S, Dasgupta S, Patra S K, Bhattacharyya K & Dey P (2021). Response of cabbage to soil test-based fertilization coupled with different levels of drip irrigation in an inceptisol. Irrigation Science 1-15. https://doi.org/10.1007/s00271-021-00761-z
- Semiz G D, Şentürk C, Yildirim A C & Torun E (2023). Modelling Yield Response and Water Use to Salinity and Water Relations of Six Pepper Varieties. Journal of Agricultural Sciences (Tarim Bilimleri Dergisi) 29(1):188-199. DOI: 10.15832/ankutbd.1017255
- Shawon R A, Kang B S, Lee S G, Kim S K, Lee H J, Katrich E, Gorinstein S & Ku Y G (2020). Influence of drought stress on bioactive compounds, antioxidant enzymes and glucosinolate contents of Chinese cabbage (Brassica rapa). Food Chemistry 308: 1–9. https://doi.org/10.1016/j.foodchem.2019.125657.
- Shinde M G, Pawar D D, Kale K D & Dingre S K (2020). Performance of cabbage at different irrigation levels under drip and micro sprinkler irrigation systems. Irrigation Drainage Published online in Wiley Online Library Cited 02 June 2021. https://onlinelibrary.wiley.com/doi/pdf/10.1002/ird.2557.
- Siro I, Kápolna E, Kápolna B & Lugasi A (2008). Functional food. Product development, marketing and consumer acceptance—A review. Appetite 51(3): 456-467
- Šola I, Stić P & Rusak G (2021). Effect of flooding and drought on the content of phenolics, sugars, photosynthetic pigments and vitamin C and antioxidant potential of young Chinese cabbage. European Food Research and Technology 247: 1913-1920. https://doi.org/10.1007/s00217-021-03759-1
- Şahin U, Ekinci M, Ors S, Turan M, Yıldız S & Yıldırım E (2018). Effects of individual and combined effects of salinity and drought on physiological, nutritional and biochemical properties of cabbage (Brassica oleracea var. capitata). Scientia Horticulturae 240: 196–204. https://doi.org/10.1016/j.scienta.2018.06.016
- Valifard M, Mohsenzadeh S & Kholdebarin B (2017). Salinity effects on phenolic content and antioxidant activity of Salvia macrosiphon. Iranian Journal of Science and Technology, Transaction A 41: 295–300. https://doi.org/10.1007/s40995-016-0022-y.
- Volden J, Borge G I A, Bengtsson G B, Hansen M, Thygesen I E & Wicklund T (2008). Effect of thermal treatment on glucosinolates and antioxidant-related parameters in red cabbage (Brassica oleracea L. ssp. capitata f. rubra). Food Chemistry 109: 595-605. https://doi.org/10.1016/j.foodchem.2008.01.010
- Wallace J S (2000). Increasing agricultural water use efficiency to meet future food production. Agriculture, Ecosystems & Environment, 82(1-3): 105-119. https://doi.org/10.1016/S0167-8809(00)00220-6
- Wiczkowski W, Szawara-Nowak D & Topolska J (2013). Red cabbage anthocyanins: profile, isolation, identification, and antioxidant activity. Food Research International 51: 303-309. https://doi.org/10.1016/j.foodres.2012.12.015
- Zhang X, Lu G, Long W, Zou X, Li F & Nishio T (2014). Recent progress in drought and salt tolerance studies in Brassica crops. Breeding Science 64: 60-73. https://doi.org/10.1270/jsbbs.64.60