Research Article
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Effect of Varying Biochar Particle Sizes and Concentrations on Soil Nutrient Retention and Microbial Activity

Year 2025, Volume: 31 Issue: 2, 288 - 301, 25.03.2025

Abstract

This study aims to determine the effect of adding biochar to soil under different management systems, as well as soil nutrient availability in a temperate environment. We tested whether biochar could enhance the chemical and biological properties of soil and reduce nutrient leaching. There were two parts of the study. These two studies were not related with each other, but the only similar study approach was the ageing effect of biochar (incubated of soil mixture for up to 300 days and 30 days). In the first part of the study, 2% of biochar by weight with a <5 mm particle size was produced from hardwood and incorporated into three different types of soil. The three types are an arable loam soil, an arable sandy soil and a grassland soil. The soils with and without biochar (control) were incubated for up to 300 days. In the second part of the study, different dosages of hardwood biochar (2% and 5%) with various particle sizes (2, 1, 0.5 and 0.1 mm) were incorporated into soils with different nutrient status (fertilised and unfertilised soils) and incubated for up to 30 days. The findings from the study exhibited that hardwood biochar significantly increased the mineralisation of 14C-glucose at 5% biochar dosage and at finer particle size. The pH of soil and carbon and the microbial biomass in unfertilised soil also increased after biochar addition. Adding biochar to soil had no major change on the ageing effect of the biochar and the leaching of nitrate ions, but reduced the ammonium ion leaching. The efficacy of biochar application depends on soil type, nutrient availability, biochar application rate and particle size.

Supporting Institution

Malaysian Agricultural Research and Development Institute and Lancaster University

References

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  • Boucard T K, McNeill C, Bardgett R D, Paynter C D & Semple K T (2008). The impact of synthetic pyrethroid and organophosphate sheep dip formulations on microbial activity in soil. Environmental Pollution 153: 207 – 214. https://doi:10.1016/j.envpol.2007.07.027
  • Chen H, Yang Z, Chu R K, Tolic N, Liang , Graham D E, Wullschleger S D & Gu B (2018). Molecular insights into arctic soil organic matter degradation under warming. Environmental Science & Technology 52(8): 4555-4564. https://doi.org/10.1021/acs.est.7b05469
  • Dynarski K A, Bossio D A & Scow K M (2020). Dynamic Stability of Soil Carbon: Reassessing the “Permanence” of Soil Carbon Sequestration. Frontiers in Environmental Science 8: 218. https://doi.org/10.3389/fenvs.2020.514701
  • de Jesus Duarte S, Glaser B & Pellegrino Cerri C E (2019). Effect of biochar particle size on physical, hydrological and chemical properties of loamy and sandy tropical soils. Agronomy 9(4): 165. https://doi.org/10.3390/agronomy9040165
  • Doick K J & Semple K T (2003). The effect of soil: water ratios on the mineralisation of phenanthrene: LNAPL mixtures in soil. FEMS Microbiology Letters 220(1): 29-33. https://doi.org/10.1016/S0378-1097(03)00056-9
  • Hamer U, Marschner B, Brodowski S & Amelung W (2004). Interactive priming of black carbon and glucose mineralisation. Organic Geochemistry 35(7): 823-830. https://doi.org/10.1016/j.orggeochem.2004.03.003
  • Ippolito J A, Cui L, Kammann C, Wrage-Mönnig N, Estavillo J M, Fuertes-Mendizabal T, Cayuela M L, Sigua G, Novak J, Spokas K & Borchard N (2020). Feedstock choice, pyrolysis temperature and type influence biochar characteristics: a comprehensive meta-data analysis review. Biochar 2: 421-438. https://doi.org/10.1007/s42773-020-00067-x
  • Jiang S, Nguyen T A, Rudolph V, Yang H, Zhang D, Ok Y S & Huang L (2017). Characterization of hard-and softwood biochars pyrolyzed at high temperature. Environmental geochemistry and Health 39: 403-415. DOI 10.1007/s10653-016-9873-6
  • Jones D L, Rousk J, Edwards-Jones G, DeLuca T H & Murphy D V (2012). Biochar-mediated changes in soil quality and plant growth in a three year field trial. Soil Biology and Biochemistry 45: 113-124. https://doi.org/10.1016/j.soilbio.2011.10.012
  • Kandeler E (2007). Physiological and biochemical methods for studying soil biota and their function. Soil Microbiology, Ecology, and Biochemistry pp. 53-83. https://doi.org/10.1016/B978-0-08-047514-1.50007-X
  • Lehmann J, da Silva Jr J P, Steiner C, Nehls T, Zech W & Glaser B (2003). Nutrient availability and leaching in an archaeological Anthrosol and a Ferralsol of the Central Amazon basin: fertilizer, manure and charcoal amendments. Plant and Soil 249(2): 343-357. https://doi.org/10.1023/A:1022833116184
  • Liang Z, Olesen J E, Jensen J L & Elsgaard L (2019). Nutrient availability affects carbon turnover and microbial physiology differently in topsoil and subsoil under a temperate grassland. Geoderma 336: 22-30. https://doi.org/10.1016/j.geoderma.2018.08.021
  • Nie C, Yang X, Niazi N K, Xu X, Wen Y, Rinklebe J, Ok Y S, Xu S & Wang H (2018). Impact of sugarcane bagasse-derived biochar on heavy metal availability and microbial activity: a field study. Chemosphere 200: 274-282. https://doi.org/10.1016/j.chemosphere.2018.02.134
  • Quilliam R S, Marsden Karina A, Gertler C, Rousk J, DeLuca T H & Jones D L (2012). Nutrient dynamics, microbial growth and weed emergence in biochar amended soil are influenced by time since applicatioan and reapplication rate. Agriculture, Ecosystem and Environment 158: 192-199. https://doi.org/10.1016/j.agee.2012.06.011
  • Paroissien J B, Darboux F, Couturier A, Devillers B, Mouillot F, Raclot D & Le Bissonnais Y (2015). A method for modeling the effects of climate and land use changes on erosion and sustainability of soil in a Mediterranean watershed (Languedoc, France). Journal of Environmental Management 150: 57-68. https://doi.org/10.1016/j.jenvman.2014.10.034
  • Reid B J, MacLeod C J, Lee P H, Morriss A W, Stokes J D & Semple K T (2001). A simple 14C-respirometric method for assessing microbial catabolic potential and contaminant bioavailability. FEMS Microbiology Letters 196(2): 141-146. https://doi.org/10.1111/j.1574- 6968.2001.tb10555.x
  • Sarfraz R, Yang W, Wang S, Zhou B & Xing S (2020). Short term effects of biochar with different particle sizes on phosphorous availability and microbial communities. Chemosphere, 256, 126862. pp https://doi.org/10.1016/ j.chemosphere.2020.126862
  • Sigua G, Novak J, Watts D, Cantrell K, Shumaker P, Szögi A & Johnson M (2014). Carbon mineralization in two ultisols amended with different sources and particle sizes of pyrolyzed biochar. Chemosphere 103: 313-321. https://doi.org/10.1016/j.chemosphere.2013.12.024
  • Wang D, Zhang W, Hao X & Zhou D (2013). Transport of biochar particles in saturated granular media: effects of pyrolysis temperature and particle size. Environmental Science & Technology 47(2): 821-828. https://doi.org/10.1021/es303794d
  • Wang W, Zeng C, Sardans J, Wang C, Zeng D & Peñuelas J (2016). Amendment with industrial and agricultural wastes reduces surface-water nutrient loss and storage of dissolved greenhouse gases in a subtropical paddy field. Agriculture, Ecosystems & Environment 231: 296- 303. https://doi.org/10.1016/j.agee.2016.07.012
  • Wong J W C, Webber J B W & Ogbonnaya U O (2019). Characteristics of biochar porosity by NMR and study of ammonium ion adsorption. Journal of Analytical and Applied Pyrolysis, 143, 104687 pp. https://doi.org/10.1016/j.jaap.2019.104687
  • Yao Y, Gao B, Zhang M, Inyang M & Zimmerman A R (2012). Effect of biochar amendment on sorption and leaching of nitrate, ammonium, and phosphate in a sandy soil. Chemosphere 89: 1467-1471. https://doi.org/10.1016/j.chemosphere.2012.06.002
  • Zhang Q.-z, Dijkstra F A, Liu X.-r, Wang Y.-d, Huang J & Lu N (2014). Effects of biochar on soil microbial biomass after four years of consecutive application in the north China plain. PloS One 9(7): p.e102062. https://doi.org/10.1371/journal.pone.0102062
  • Zhang J, Tang X, Zhong S, Yin G, Gao Y & He X (2017). Recalcitrant carbon components in glomalin-related soil protein facilitate soil organic carbon preservation in tropical forests. Scientific Reports 7(1): 1-9. DOI:10.1038/s41598-017-02486-6
  • Zhang M, Song G, Gelardi D L, Huang L, Khan E, Mašek O, Parikh S J & Ok Y S (2020). Evaluating biochar and its modifications for the removal of ammonium, nitrate, and phosphate in water. Water Research, 116303 pp. https://doi.org/10.1016/j.watres.2020.116303
  • Zhao R, Wu J, Jiang C & Liu F (2020). Effects of biochar particle size and concomitant nitrogen fertilization on soil microbial community structure during the maize seedling stage. Environmental Science and Pollution Research 27(12): 13095- 13104. https://doi.org/10.1007/s11356-020-07888-0
  • Zhou J, Liang X, Shan S, Yan D, Chen Y, Yang C, Lu Y, Niyungeko C & Tian G (2019). Nutrient retention by different substrates from an improved low impact development system. Journal of Environmental Management 238: 331-340. https://doi.org/10.1016/j.jenvman.2019.03.019
Year 2025, Volume: 31 Issue: 2, 288 - 301, 25.03.2025

Abstract

References

  • Busch D & Glaser B (2015). Stability of co‐composted hydrochar and biochar under field conditions in a temperate soil. Soil Use and Management 31(2): 251-258. https://doi.org/10.1111/sum.12180
  • Boucard T K, McNeill C, Bardgett R D, Paynter C D & Semple K T (2008). The impact of synthetic pyrethroid and organophosphate sheep dip formulations on microbial activity in soil. Environmental Pollution 153: 207 – 214. https://doi:10.1016/j.envpol.2007.07.027
  • Chen H, Yang Z, Chu R K, Tolic N, Liang , Graham D E, Wullschleger S D & Gu B (2018). Molecular insights into arctic soil organic matter degradation under warming. Environmental Science & Technology 52(8): 4555-4564. https://doi.org/10.1021/acs.est.7b05469
  • Dynarski K A, Bossio D A & Scow K M (2020). Dynamic Stability of Soil Carbon: Reassessing the “Permanence” of Soil Carbon Sequestration. Frontiers in Environmental Science 8: 218. https://doi.org/10.3389/fenvs.2020.514701
  • de Jesus Duarte S, Glaser B & Pellegrino Cerri C E (2019). Effect of biochar particle size on physical, hydrological and chemical properties of loamy and sandy tropical soils. Agronomy 9(4): 165. https://doi.org/10.3390/agronomy9040165
  • Doick K J & Semple K T (2003). The effect of soil: water ratios on the mineralisation of phenanthrene: LNAPL mixtures in soil. FEMS Microbiology Letters 220(1): 29-33. https://doi.org/10.1016/S0378-1097(03)00056-9
  • Hamer U, Marschner B, Brodowski S & Amelung W (2004). Interactive priming of black carbon and glucose mineralisation. Organic Geochemistry 35(7): 823-830. https://doi.org/10.1016/j.orggeochem.2004.03.003
  • Ippolito J A, Cui L, Kammann C, Wrage-Mönnig N, Estavillo J M, Fuertes-Mendizabal T, Cayuela M L, Sigua G, Novak J, Spokas K & Borchard N (2020). Feedstock choice, pyrolysis temperature and type influence biochar characteristics: a comprehensive meta-data analysis review. Biochar 2: 421-438. https://doi.org/10.1007/s42773-020-00067-x
  • Jiang S, Nguyen T A, Rudolph V, Yang H, Zhang D, Ok Y S & Huang L (2017). Characterization of hard-and softwood biochars pyrolyzed at high temperature. Environmental geochemistry and Health 39: 403-415. DOI 10.1007/s10653-016-9873-6
  • Jones D L, Rousk J, Edwards-Jones G, DeLuca T H & Murphy D V (2012). Biochar-mediated changes in soil quality and plant growth in a three year field trial. Soil Biology and Biochemistry 45: 113-124. https://doi.org/10.1016/j.soilbio.2011.10.012
  • Kandeler E (2007). Physiological and biochemical methods for studying soil biota and their function. Soil Microbiology, Ecology, and Biochemistry pp. 53-83. https://doi.org/10.1016/B978-0-08-047514-1.50007-X
  • Lehmann J, da Silva Jr J P, Steiner C, Nehls T, Zech W & Glaser B (2003). Nutrient availability and leaching in an archaeological Anthrosol and a Ferralsol of the Central Amazon basin: fertilizer, manure and charcoal amendments. Plant and Soil 249(2): 343-357. https://doi.org/10.1023/A:1022833116184
  • Liang Z, Olesen J E, Jensen J L & Elsgaard L (2019). Nutrient availability affects carbon turnover and microbial physiology differently in topsoil and subsoil under a temperate grassland. Geoderma 336: 22-30. https://doi.org/10.1016/j.geoderma.2018.08.021
  • Nie C, Yang X, Niazi N K, Xu X, Wen Y, Rinklebe J, Ok Y S, Xu S & Wang H (2018). Impact of sugarcane bagasse-derived biochar on heavy metal availability and microbial activity: a field study. Chemosphere 200: 274-282. https://doi.org/10.1016/j.chemosphere.2018.02.134
  • Quilliam R S, Marsden Karina A, Gertler C, Rousk J, DeLuca T H & Jones D L (2012). Nutrient dynamics, microbial growth and weed emergence in biochar amended soil are influenced by time since applicatioan and reapplication rate. Agriculture, Ecosystem and Environment 158: 192-199. https://doi.org/10.1016/j.agee.2012.06.011
  • Paroissien J B, Darboux F, Couturier A, Devillers B, Mouillot F, Raclot D & Le Bissonnais Y (2015). A method for modeling the effects of climate and land use changes on erosion and sustainability of soil in a Mediterranean watershed (Languedoc, France). Journal of Environmental Management 150: 57-68. https://doi.org/10.1016/j.jenvman.2014.10.034
  • Reid B J, MacLeod C J, Lee P H, Morriss A W, Stokes J D & Semple K T (2001). A simple 14C-respirometric method for assessing microbial catabolic potential and contaminant bioavailability. FEMS Microbiology Letters 196(2): 141-146. https://doi.org/10.1111/j.1574- 6968.2001.tb10555.x
  • Sarfraz R, Yang W, Wang S, Zhou B & Xing S (2020). Short term effects of biochar with different particle sizes on phosphorous availability and microbial communities. Chemosphere, 256, 126862. pp https://doi.org/10.1016/ j.chemosphere.2020.126862
  • Sigua G, Novak J, Watts D, Cantrell K, Shumaker P, Szögi A & Johnson M (2014). Carbon mineralization in two ultisols amended with different sources and particle sizes of pyrolyzed biochar. Chemosphere 103: 313-321. https://doi.org/10.1016/j.chemosphere.2013.12.024
  • Wang D, Zhang W, Hao X & Zhou D (2013). Transport of biochar particles in saturated granular media: effects of pyrolysis temperature and particle size. Environmental Science & Technology 47(2): 821-828. https://doi.org/10.1021/es303794d
  • Wang W, Zeng C, Sardans J, Wang C, Zeng D & Peñuelas J (2016). Amendment with industrial and agricultural wastes reduces surface-water nutrient loss and storage of dissolved greenhouse gases in a subtropical paddy field. Agriculture, Ecosystems & Environment 231: 296- 303. https://doi.org/10.1016/j.agee.2016.07.012
  • Wong J W C, Webber J B W & Ogbonnaya U O (2019). Characteristics of biochar porosity by NMR and study of ammonium ion adsorption. Journal of Analytical and Applied Pyrolysis, 143, 104687 pp. https://doi.org/10.1016/j.jaap.2019.104687
  • Yao Y, Gao B, Zhang M, Inyang M & Zimmerman A R (2012). Effect of biochar amendment on sorption and leaching of nitrate, ammonium, and phosphate in a sandy soil. Chemosphere 89: 1467-1471. https://doi.org/10.1016/j.chemosphere.2012.06.002
  • Zhang Q.-z, Dijkstra F A, Liu X.-r, Wang Y.-d, Huang J & Lu N (2014). Effects of biochar on soil microbial biomass after four years of consecutive application in the north China plain. PloS One 9(7): p.e102062. https://doi.org/10.1371/journal.pone.0102062
  • Zhang J, Tang X, Zhong S, Yin G, Gao Y & He X (2017). Recalcitrant carbon components in glomalin-related soil protein facilitate soil organic carbon preservation in tropical forests. Scientific Reports 7(1): 1-9. DOI:10.1038/s41598-017-02486-6
  • Zhang M, Song G, Gelardi D L, Huang L, Khan E, Mašek O, Parikh S J & Ok Y S (2020). Evaluating biochar and its modifications for the removal of ammonium, nitrate, and phosphate in water. Water Research, 116303 pp. https://doi.org/10.1016/j.watres.2020.116303
  • Zhao R, Wu J, Jiang C & Liu F (2020). Effects of biochar particle size and concomitant nitrogen fertilization on soil microbial community structure during the maize seedling stage. Environmental Science and Pollution Research 27(12): 13095- 13104. https://doi.org/10.1007/s11356-020-07888-0
  • Zhou J, Liang X, Shan S, Yan D, Chen Y, Yang C, Lu Y, Niyungeko C & Tian G (2019). Nutrient retention by different substrates from an improved low impact development system. Journal of Environmental Management 238: 331-340. https://doi.org/10.1016/j.jenvman.2019.03.019
There are 28 citations in total.

Details

Primary Language English
Subjects Plant Nutrition and Soil Fertility
Journal Section Makaleler
Authors

Khasifah Muhamad 0000-0001-8937-2625

Uchenna Ogbonnaya This is me 0000-0002-6906-7823

Kirk Semple This is me 0000-0002-4046-2037

John Quinton This is me 0000-0003-1746-4795

Publication Date March 25, 2025
Submission Date May 21, 2024
Acceptance Date October 10, 2024
Published in Issue Year 2025 Volume: 31 Issue: 2

Cite

APA Muhamad, K., Ogbonnaya, U., Semple, K., Quinton, J. (2025). Effect of Varying Biochar Particle Sizes and Concentrations on Soil Nutrient Retention and Microbial Activity. Journal of Agricultural Sciences, 31(2), 288-301.
AMA Muhamad K, Ogbonnaya U, Semple K, Quinton J. Effect of Varying Biochar Particle Sizes and Concentrations on Soil Nutrient Retention and Microbial Activity. J Agr Sci-Tarim Bili. March 2025;31(2):288-301.
Chicago Muhamad, Khasifah, Uchenna Ogbonnaya, Kirk Semple, and John Quinton. “Effect of Varying Biochar Particle Sizes and Concentrations on Soil Nutrient Retention and Microbial Activity”. Journal of Agricultural Sciences 31, no. 2 (March 2025): 288-301.
EndNote Muhamad K, Ogbonnaya U, Semple K, Quinton J (March 1, 2025) Effect of Varying Biochar Particle Sizes and Concentrations on Soil Nutrient Retention and Microbial Activity. Journal of Agricultural Sciences 31 2 288–301.
IEEE K. Muhamad, U. Ogbonnaya, K. Semple, and J. Quinton, “Effect of Varying Biochar Particle Sizes and Concentrations on Soil Nutrient Retention and Microbial Activity”, J Agr Sci-Tarim Bili, vol. 31, no. 2, pp. 288–301, 2025.
ISNAD Muhamad, Khasifah et al. “Effect of Varying Biochar Particle Sizes and Concentrations on Soil Nutrient Retention and Microbial Activity”. Journal of Agricultural Sciences 31/2 (March 2025), 288-301.
JAMA Muhamad K, Ogbonnaya U, Semple K, Quinton J. Effect of Varying Biochar Particle Sizes and Concentrations on Soil Nutrient Retention and Microbial Activity. J Agr Sci-Tarim Bili. 2025;31:288–301.
MLA Muhamad, Khasifah et al. “Effect of Varying Biochar Particle Sizes and Concentrations on Soil Nutrient Retention and Microbial Activity”. Journal of Agricultural Sciences, vol. 31, no. 2, 2025, pp. 288-01.
Vancouver Muhamad K, Ogbonnaya U, Semple K, Quinton J. Effect of Varying Biochar Particle Sizes and Concentrations on Soil Nutrient Retention and Microbial Activity. J Agr Sci-Tarim Bili. 2025;31(2):288-301.

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