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Resveratrolün diyabetik sıçanlarda tendon iyileşmesi üzerine etkileri

Year 2014, , 355 - 362, 30.05.2014
https://doi.org/10.3944/AOTT.2014.13.0096

Abstract

Amaç:Bu çalışma streptozotosin ile diyabetik hale getirilmiş sıçanlarda resveratrolün tendon iyileşmesi üzerine etkilerini göstermek için planlandı.

Çalışma planı: Streptozotosin indüklemesi ile diyabet oluşturulan 16 adet Sprague-Dawley suşu erkek sıçanın Aşil tendonlarına bilateral tenorafi yapıldı. Cerrahi sonrası dönemde deney grubuna (n=8) intraperitoneal resveratrol, kontrol grubuna (n=8) intraperitoneal salin enjeksiyonu yapıldı. Sıçanlar 14 gün sonra sakrifiye edildi. Sağ taraf tendonları biyomekanik olarak, sol taraf tendonları ise histolojik olarak incelendi.

Bulgular: Tendonların kopmadan önce dayanabildikleri ortalama yük açısından gruplar arasında istatistiksel anlamlı fark saptanmadı (p>0.05). Histolojik incelemede ise tendon onarım bölgesinde yeni sentezlenen kollajen konfigürasyonunun deney grubunda daha düzenli olduğu görüldü. Yeni sentezlenen kollajen bölge oranının deney grubunda kontrol grubuna oranla daha yüksek olduğu ve aradaki farkın istatistiksel olarak anlamlı olduğu görüldü (p<0.01).

Çıkarımlar: Resveratrolün diyabetik koşullarda tendon iyileşmesi üzerine ilk 14 gün içinde pozitif etkisi olduğu bulunmuştur.

References

  • Egemen O, Ozkaya O, Ozturk MB, Sen E, Akan M, Sakiz D, et al. The biomechanical and histological effects of diabetes on tendon healing: experimental study in rats. J Hand Microsurg 2012;4:60-4.
  • Fonseca-Kelly Z, Nassrallah M, Uribe J, Khan RS, Dine K, Dutt M, et al. Resveratrol neuroprotection in a chronic mouse model of multiple sclerosis. Front Neurol 2012;3:84.
  • Leon-Galicia I, Diaz-Chavez J, Garcia-Villa E, UribeFigueroa L, Hidalgo-Miranda A, Herrera LA, et al. Resveratrol induces downregulation of DNA repair genes in MCF-7 human breast cancer cells. Eur J Cancer Prev 2013;22:11-20.
  • Minakawa M, Miura Y, Yagasaki K. Piceatannol, a resveratrol derivative, promotes glucose uptake through glucose transporter 4 translocation to plasma membrane in L6 myocytes and suppresses blood glucose levels in type 2 diabetic model db/db mice. Biochem Biophys Res Commun 2012;422:469-75.
  • Rahal K, Schmiedlin-Ren P, Adler J, Dhanani M, Sultani V, Rittershaus AC, et al. Resveratrol has antiinflammatory and antifibrotic effects in the peptidoglycan-polysaccharide rat model of Crohn’s disease. Inflamm Bowel Dis 2012;18:613-23.
  • Aiyegbusi AI, Duru FI, Awelimobor D, Noronha CC, Okanlawon AO. The role of aqueous extract of pineapple fruit parts on the healing of acute crush tendon injury. Nig Q J Hosp Med 2010;20:223-7.
  • Omeroğlu S, Peker T, Türközkan N, Omeroğlu H. Highdose vitamin C supplementation accelerates the Achilles tendon healing in healthy rats. Arch Orthop Trauma Surg 2009;129:281-6.
  • İrer SV, Alper G. Experimental diabetes models. [Article in Turkish] Türk Klinik Biyokimya Derg 2004;2:127-36.
  • Ozgenel GY, Samli B, Ozcan M. Effects of human amniotic fluid on peritendinous adhesion formation and tendon healing after flexor tendon surgery in rabbits. J Hand Surg Am 2001;26:332-9.
  • Broughton G 2nd, Janis JE, Attinger CE. Wound healing: an overview. Plast Reconstr Surg 2006;117(7 Suppl):1eS-32e-S.
  • He Z, King GL. Microvascular complications of diabetes. Endocrinol Metab Clin North Am 2004;33:215-38.
  • Babu PV, Sabitha KE, Shyamaladevi CS. Effect of green tea extract on advanced glycation and cross-linking of tail tendon collagen in streptozotocin induced diabetic rats. Food Chem Toxicol 2008;46:280-5.
  • Ahmed AS, Schizas N, Li J, Ahmed M, Östenson CG, Salo P, et al. Type 2 diabetes impairs tendon repair after injury in a rat model. J Appl Physiol (1985) 2012;113:1784
  • Nouruzian M, Alidoust M, Bayat M, Bayat M, Akbari M. Effect of low-level laser therapy on healing of tenotomized Achilles tendon in streptozotocin-induced diabetic rats. Lasers Med Sci 2013;28:399-405. de Oliveira RR, Bezerra MA, de Lira KD, Novaes KA, Teixeira MF, Chaves Cde C, et al. Aerobic physical training restores biomechanical properties of Achilles tendon in rats chemically induced to diabetes mellitus. J Diabetes Complications 2012;26:163-8.
  • Langcake P, Pryce RJ. A new class of phytoalexins from grapevines. Experientia 1977;33:151-2.
  • Aribal-Kocatürk P, Kavas GO, Büyükkağnici DI. Pretreatment effect of resveratrol on streptozotocin-induced diabetes in rats. Biol Trace Elem Res 2007;118:244-9.
  • Lekli I, Szabo G, Juhasz B, Das S, Das M, Varga E, et al. Protective mechanisms of resveratrol against ischemia-reperfusion-induced damage in hearts obtained from Zucker obese rats: the role of GLUT-4 and endothelin. Am J Physiol Heart Circ Physiol 2008;294:H859-66.
  • Su HC, Hung LM, Chen JK. Resveratrol, a red wine antioxidant, possesses an insulin-like effect in streptozotocininduced diabetic rats. Am J Physiol Endocrinol Metab 2006;290:E1339-46.
  • Palsamy P, Subramanian S. Resveratrol, a natural phytoalexin, normalizes hyperglycemia in streptozotocinnicotinamide induced experimental diabetic rats. Biomed Pharmacother 2008;62:598-605.
  • Penumathsa SV, Thirunavukkarasu M, Zhan L, Maulik G, Menon VP, Bagchi D, et al. Resveratrol enhances GLUT-4 translocation to the caveolar lipid raft fractions through AMPK/Akt/eNOS signalling pathway in diabetic myocardium. J Cell Mol Med 2008;12:2350-61.
  • Esen E, Cila E, Ozoğul C, Taşçi AG, Sipahioğlu S, Gemalmaz HC, et al. The effect of low-molecular-weight heparin on rat tendon healing. Acta Orthop Traumatol Turc 2009;43:54-61.
  • Hueper K, Hartung D, Gutberlet M, Gueler F, Sann H, Husen B, et al. Magnetic resonance diffusion tensor imaging for evaluation of histopathological changes in a rat model of diabetic nephropathy. Invest Radiol 2012;47:430-7.
  • Isken T, Serdaroglu I, Ozgentas E. The effects of the pentoxifylline on survival of the skin flaps in streptozotocindiabetic rats. Ann Plast Surg 2009;62:446-50.
  • Chan BP, Fu SC, Qin L, Rolf C, Chan KM. Supplementation-time dependence of growth factors in promoting tendon healing. Clin Orthop Relat Res 2006;448:240-7.
  • Lyras DN, Kazakos K, Georgiadis G, Mazis G, Middleton R, Richards S, et al. Does a single application of PRP alter the expression of IGF-I in the early phase of tendon healing? J Foot Ankle Surg 2011;50:276-82.
  • Majewski M, Betz O, Ochsner PE, Liu F, Porter RM, Evans CH. Ex vivo adenoviral transfer of bone morphogenetic protein 12 (BMP-12) cDNA improves Achilles tendon healing in a rat model. Gene Ther 2008;15:1139-46. Majewski M, Ochsner PE, Liu F, Flückiger R, Evans CH. Accelerated healing of the rat Achilles tendon in response to autologous conditioned serum. Am J Sports Med 2009;37:2117-25.
  • Zhang F, Liu H, Stile F, Lei MP, Pang Y, Oswald TM, et al. Effect of vascular endothelial growth factor on rat Achilles tendon healing. Plast Reconstr Surg 2003;112:1613-9.
  • Busch F, Mobasheri A, Shayan P, Stahlmann R, Shakibaei M. Sirt-1 is required for the inhibition of apoptosis and inflammatory responses in human tenocytes. J Biol Chem 2012;287:25770-81.

The effects of resveratrol on tendon healing of diabetic rats

Year 2014, , 355 - 362, 30.05.2014
https://doi.org/10.3944/AOTT.2014.13.0096

Abstract

Objective:The aim of this study was to determine the effects of resveratrol on the tendon healing process of streptozotocin-induced diabetic rats.

Methods: The study included 16 male Sprague-Dawley rats. Streptozotocin was administered to induce diabetes and bilateral tenorrhaphy of the Achilles tendons was performed. Intraperitoneal resveratrol was injected in the experimental group (n=8) and saline in the control group (n=8) during the postoperative period. Rats were sacrificed at the 14th day. Right side tendons were evaluated biomechanically and left side tendons histologically.

Results: Difference in mean tendon tensile strength was not statistically significant between groups (p>0.05). Histologic evaluations of the repair zones showed greater configuration of the newly synthesized collagen in the experimental group. The ratio of the newly synthesized collagen area to the healing region area was significantly higher in the experimental group then the control group (p<0.01).

Conclusion: Resveratrol appears to have a positive impact on the process of tendon healing in diabetic conditions in the first 14 days.

References

  • Egemen O, Ozkaya O, Ozturk MB, Sen E, Akan M, Sakiz D, et al. The biomechanical and histological effects of diabetes on tendon healing: experimental study in rats. J Hand Microsurg 2012;4:60-4.
  • Fonseca-Kelly Z, Nassrallah M, Uribe J, Khan RS, Dine K, Dutt M, et al. Resveratrol neuroprotection in a chronic mouse model of multiple sclerosis. Front Neurol 2012;3:84.
  • Leon-Galicia I, Diaz-Chavez J, Garcia-Villa E, UribeFigueroa L, Hidalgo-Miranda A, Herrera LA, et al. Resveratrol induces downregulation of DNA repair genes in MCF-7 human breast cancer cells. Eur J Cancer Prev 2013;22:11-20.
  • Minakawa M, Miura Y, Yagasaki K. Piceatannol, a resveratrol derivative, promotes glucose uptake through glucose transporter 4 translocation to plasma membrane in L6 myocytes and suppresses blood glucose levels in type 2 diabetic model db/db mice. Biochem Biophys Res Commun 2012;422:469-75.
  • Rahal K, Schmiedlin-Ren P, Adler J, Dhanani M, Sultani V, Rittershaus AC, et al. Resveratrol has antiinflammatory and antifibrotic effects in the peptidoglycan-polysaccharide rat model of Crohn’s disease. Inflamm Bowel Dis 2012;18:613-23.
  • Aiyegbusi AI, Duru FI, Awelimobor D, Noronha CC, Okanlawon AO. The role of aqueous extract of pineapple fruit parts on the healing of acute crush tendon injury. Nig Q J Hosp Med 2010;20:223-7.
  • Omeroğlu S, Peker T, Türközkan N, Omeroğlu H. Highdose vitamin C supplementation accelerates the Achilles tendon healing in healthy rats. Arch Orthop Trauma Surg 2009;129:281-6.
  • İrer SV, Alper G. Experimental diabetes models. [Article in Turkish] Türk Klinik Biyokimya Derg 2004;2:127-36.
  • Ozgenel GY, Samli B, Ozcan M. Effects of human amniotic fluid on peritendinous adhesion formation and tendon healing after flexor tendon surgery in rabbits. J Hand Surg Am 2001;26:332-9.
  • Broughton G 2nd, Janis JE, Attinger CE. Wound healing: an overview. Plast Reconstr Surg 2006;117(7 Suppl):1eS-32e-S.
  • He Z, King GL. Microvascular complications of diabetes. Endocrinol Metab Clin North Am 2004;33:215-38.
  • Babu PV, Sabitha KE, Shyamaladevi CS. Effect of green tea extract on advanced glycation and cross-linking of tail tendon collagen in streptozotocin induced diabetic rats. Food Chem Toxicol 2008;46:280-5.
  • Ahmed AS, Schizas N, Li J, Ahmed M, Östenson CG, Salo P, et al. Type 2 diabetes impairs tendon repair after injury in a rat model. J Appl Physiol (1985) 2012;113:1784
  • Nouruzian M, Alidoust M, Bayat M, Bayat M, Akbari M. Effect of low-level laser therapy on healing of tenotomized Achilles tendon in streptozotocin-induced diabetic rats. Lasers Med Sci 2013;28:399-405. de Oliveira RR, Bezerra MA, de Lira KD, Novaes KA, Teixeira MF, Chaves Cde C, et al. Aerobic physical training restores biomechanical properties of Achilles tendon in rats chemically induced to diabetes mellitus. J Diabetes Complications 2012;26:163-8.
  • Langcake P, Pryce RJ. A new class of phytoalexins from grapevines. Experientia 1977;33:151-2.
  • Aribal-Kocatürk P, Kavas GO, Büyükkağnici DI. Pretreatment effect of resveratrol on streptozotocin-induced diabetes in rats. Biol Trace Elem Res 2007;118:244-9.
  • Lekli I, Szabo G, Juhasz B, Das S, Das M, Varga E, et al. Protective mechanisms of resveratrol against ischemia-reperfusion-induced damage in hearts obtained from Zucker obese rats: the role of GLUT-4 and endothelin. Am J Physiol Heart Circ Physiol 2008;294:H859-66.
  • Su HC, Hung LM, Chen JK. Resveratrol, a red wine antioxidant, possesses an insulin-like effect in streptozotocininduced diabetic rats. Am J Physiol Endocrinol Metab 2006;290:E1339-46.
  • Palsamy P, Subramanian S. Resveratrol, a natural phytoalexin, normalizes hyperglycemia in streptozotocinnicotinamide induced experimental diabetic rats. Biomed Pharmacother 2008;62:598-605.
  • Penumathsa SV, Thirunavukkarasu M, Zhan L, Maulik G, Menon VP, Bagchi D, et al. Resveratrol enhances GLUT-4 translocation to the caveolar lipid raft fractions through AMPK/Akt/eNOS signalling pathway in diabetic myocardium. J Cell Mol Med 2008;12:2350-61.
  • Esen E, Cila E, Ozoğul C, Taşçi AG, Sipahioğlu S, Gemalmaz HC, et al. The effect of low-molecular-weight heparin on rat tendon healing. Acta Orthop Traumatol Turc 2009;43:54-61.
  • Hueper K, Hartung D, Gutberlet M, Gueler F, Sann H, Husen B, et al. Magnetic resonance diffusion tensor imaging for evaluation of histopathological changes in a rat model of diabetic nephropathy. Invest Radiol 2012;47:430-7.
  • Isken T, Serdaroglu I, Ozgentas E. The effects of the pentoxifylline on survival of the skin flaps in streptozotocindiabetic rats. Ann Plast Surg 2009;62:446-50.
  • Chan BP, Fu SC, Qin L, Rolf C, Chan KM. Supplementation-time dependence of growth factors in promoting tendon healing. Clin Orthop Relat Res 2006;448:240-7.
  • Lyras DN, Kazakos K, Georgiadis G, Mazis G, Middleton R, Richards S, et al. Does a single application of PRP alter the expression of IGF-I in the early phase of tendon healing? J Foot Ankle Surg 2011;50:276-82.
  • Majewski M, Betz O, Ochsner PE, Liu F, Porter RM, Evans CH. Ex vivo adenoviral transfer of bone morphogenetic protein 12 (BMP-12) cDNA improves Achilles tendon healing in a rat model. Gene Ther 2008;15:1139-46. Majewski M, Ochsner PE, Liu F, Flückiger R, Evans CH. Accelerated healing of the rat Achilles tendon in response to autologous conditioned serum. Am J Sports Med 2009;37:2117-25.
  • Zhang F, Liu H, Stile F, Lei MP, Pang Y, Oswald TM, et al. Effect of vascular endothelial growth factor on rat Achilles tendon healing. Plast Reconstr Surg 2003;112:1613-9.
  • Busch F, Mobasheri A, Shayan P, Stahlmann R, Shakibaei M. Sirt-1 is required for the inhibition of apoptosis and inflammatory responses in human tenocytes. J Biol Chem 2012;287:25770-81.
There are 28 citations in total.

Details

Primary Language English
Subjects Health Care Administration
Journal Section Experimental Study
Authors

Kayhan Zeytin This is me

Nesibe Sinem Ciloglu This is me

Filiz Ates This is me

Fugen Vardar Aker This is me

Feriha Ercan This is me

Publication Date May 30, 2014
Published in Issue Year 2014

Cite

APA Zeytin, K., Sinem Ciloglu, N., Ates, F., Vardar Aker, F., et al. (2014). The effects of resveratrol on tendon healing of diabetic rats. Acta Orthopaedica Et Traumatologica Turcica, 48(3), 355-362. https://doi.org/10.3944/AOTT.2014.13.0096
AMA Zeytin K, Sinem Ciloglu N, Ates F, Vardar Aker F, Ercan F. The effects of resveratrol on tendon healing of diabetic rats. Acta Orthopaedica et Traumatologica Turcica. May 2014;48(3):355-362. doi:10.3944/AOTT.2014.13.0096
Chicago Zeytin, Kayhan, Nesibe Sinem Ciloglu, Filiz Ates, Fugen Vardar Aker, and Feriha Ercan. “The Effects of Resveratrol on Tendon Healing of Diabetic Rats”. Acta Orthopaedica Et Traumatologica Turcica 48, no. 3 (May 2014): 355-62. https://doi.org/10.3944/AOTT.2014.13.0096.
EndNote Zeytin K, Sinem Ciloglu N, Ates F, Vardar Aker F, Ercan F (May 1, 2014) The effects of resveratrol on tendon healing of diabetic rats. Acta Orthopaedica et Traumatologica Turcica 48 3 355–362.
IEEE K. Zeytin, N. Sinem Ciloglu, F. Ates, F. Vardar Aker, and F. Ercan, “The effects of resveratrol on tendon healing of diabetic rats”, Acta Orthopaedica et Traumatologica Turcica, vol. 48, no. 3, pp. 355–362, 2014, doi: 10.3944/AOTT.2014.13.0096.
ISNAD Zeytin, Kayhan et al. “The Effects of Resveratrol on Tendon Healing of Diabetic Rats”. Acta Orthopaedica et Traumatologica Turcica 48/3 (May 2014), 355-362. https://doi.org/10.3944/AOTT.2014.13.0096.
JAMA Zeytin K, Sinem Ciloglu N, Ates F, Vardar Aker F, Ercan F. The effects of resveratrol on tendon healing of diabetic rats. Acta Orthopaedica et Traumatologica Turcica. 2014;48:355–362.
MLA Zeytin, Kayhan et al. “The Effects of Resveratrol on Tendon Healing of Diabetic Rats”. Acta Orthopaedica Et Traumatologica Turcica, vol. 48, no. 3, 2014, pp. 355-62, doi:10.3944/AOTT.2014.13.0096.
Vancouver Zeytin K, Sinem Ciloglu N, Ates F, Vardar Aker F, Ercan F. The effects of resveratrol on tendon healing of diabetic rats. Acta Orthopaedica et Traumatologica Turcica. 2014;48(3):355-62.