DNA-binding and spectroscopic studies of cobalt (II) compound containing 2, 10, 16, 24-tetrakis 4-(2-phenoxy-1, 3-dioxo-2, 3-dihydro-1H-inden-2-yloxy) phthalonitrile

Abstract

In this study, binding activities of the previously synthesized cobalt (II) compound (PcCo) with calf thymus-DNA containing 2, 10, 16, 24-tetrakis 4-(2-phenoxy-1, 3-dioxo-2, 3-dihydro-1H-inden-2-yloxy) phthalonitrile were investigated to determine binding activities by using UV-Vis absorption, fluorescence titration methods. Absorption titration spectra of PcCo in DMF give peaks at about 670 nm for Q-band absorption and about 340 nm for B-band absorption in the absence of calf thymus-DNA. Emission intensities of PcCo in presence of calf thymus-DNA, PcCo gives emission at about 545 nm. Thermal denaturation profile study was systematically studied by UV/Vis spectroscopic technique and melting point temperaturewas found around 7.87 oC for Co (II) phthalocyanine compound.  The reduction oxidation behavior of PcCo with calf thymus-DNA was investigated using cyclic voltammetry (CV). Cathodic peak potential and anodic peak potential for PcCo were recorded to be 0.84, -0.36 V for EPc and 0.88 V, 0.15 V, and -0.69 V EPa. The cyclic voltammetric results show one half- reversible reduction oxidation wave. The shift in Epc and Epa potentials indicates that PcCo shows strong interaction with calf thymus-DNA. Further to understand binding activities of PcCo with calf thymus-DNA was studied by using electrophoresis technique. Electrophoresis findings showed that there is decrease in the intensity of CT-DNA bands. All the findings show that PcCo interacts strongly with DNA molecule via an intercaltion binding.

Keywords

• Phthalocyanines
• DNA-binding
• UV/Vis spectroscopy
• gel electrophoresis
• cyclic voltammetry.

2, 10, 16, 24-Tetrakis 4- (2-fenoksi-l, 3-diokso-2,3-dihidro-lH-inden-2-iloksi) ftalonitril içeren kobalt (II) bileşiğinin DNA bağlanmasının spektroskopik yöntemler ile araştırılması

Abstract

2,10, 16, 24-tetrakis 4- (2-fenoksi-1,3-diokso-2,3-dihidro-lH-inden-2-iloksi) ftalonitril içeren önceden sentezlenmiş kobalt (II) bileşiğinin (PcCo) CT-DNA ile bağlanma özellikleri UV/Vis absorpsiyon, floresan titrasyonu yöntemleri kullanılarak bağlanma özelliklerini belirlemek için araştırıldı. Termal denatürasyon profili çalışması UV-Vis spektroskopik tekniği ile sistematik olarak incelenmiş ve Co (II) ftalosiyanin bileşiği için erime noktası sıcaklık değeri 7.87 oC civarında bulunmuştur. CT-DNA yokluğunda ve varlığında PcCo'in redüksiyon oksidasyon davranışı, dönüşümlü voltametri (CV) ile incelendi. Dönüşümlü (cyclic) voltametri sonuçlar, bir yarı-tersinir redaksiyon oksidasyon dalgasını göstermektedir. Epc ve Epa potansiyelindeki değişim, PcCo'in CT-DNA'yla güçlü etkileşim gösterdiğini önermektedir. PcCo'in CT-DNA ile olan etkileşimi ayrıca jel elektroforez yöntemi kullanılarak araştırılmıştır. Jel elektroforezi sonuçları, CT-DNA bantlarının yoğunluğunda azalma olduğunu gösterdi. Tüm bulgular, PcCo ile CT-DNA molekülü arasındaki etkileşimin interkalatif bağlanma türü olduğunu göstermektedir.

Keywords

• DNA
• DNA-bağlanma
• Ftalosiyaninler
• Jel Elektroforez
• UV/Vis spektroskopi

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