Research Article
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LC-DAD-ESI-MS/MS characterization of soybean phenolics extracted with various solvents

Year 2018, Volume 1, Issue 2, 70 - 78, 31.12.2018

Abstract

Phenolics are broadly distributed in the plant kingdom and the most abundant secondary metabolites of plants. Over the past few years, preparation of phenolic extracts from different plants, purification of phenolic compounds and their identification have become a major area of health- and medical-related research. In this study, the effect of extraction solvents (methanol, ethanol, acetonitrile and acetone), on total phenolic content, antioxidant activity, and the composition of the phenolic compounds in soybean extracts were studied. The antioxidant activity was determined using two different methods: DPPH and ABTS assays. Liquid chromatography coupled to diode array detection and electrospray ionisation tandem mass spectrometry (LC-DAD-ESI-MS/MS) was used for identification and quantification of phenolic compounds. All the parameters analyzed were found to be affected by the extraction method, especially by the solvent used, and the best results were obtained in the methanolic extract. The methanolic and ethanolic extracts exhibited strong antioxidant activity. Genistein-7-diglucoside, daidzin, genistin, 4’ 7 dihidroxiflavan, daidzein, and genistein were found as major isoflavonoids in all extracts.

References

  • [1] Lee J.H., Jeon J.Y., Kim S.G., Kim S.H., Chun T., Imm J.Y., Comparative analyses of total phenols, flavonoids, saponins and antioxidant activity in yellow soy beans and mung beans. Int. J. Food Sci. Tech., 2011, 46, 2513–2519. [2] Nagata C., Takatsuka N., Inaba S., Kawakawi N., Shimizu H., Effect of soymilk consumption on serum estrogen concentrations in premenopausal Japanese women. J. Natl. Cancer Inst., 1998, 90, 1830–1835. [3] Dueñas, M., Hernández, T., Robredo, S., Lamparski, G., Estrella, I., Muñoz, R. (2012). Bioactive Phenolic Compounds of Soybean (Glycine max cv. Merit): Modifi cations by Different Microbiological Fermentations. Pol. J. Food Nutr. Sci., 62, 4, 241-250. [4] Wang, H. J., Murphy, P. A. Isoflavone content in commercial soybean foods. J. Agric. Food Chem. 1994, 42, 1666−1673. [5] Li, Y., Kong, D., Bao, B., Ahmad, A., Sarkar, F. H. Induction of cancer cell death by isoflavone: the role of multiple signaling pathways. Nutrition 2011, 3, 877−896. [6] Albertazzi, P., Purdie, D. W. The nature and utility of the phytoestrogens: a review of the evidence. Maturitas 2002, 42, 173−185. [7] Lee, S.J., Seguin, P., Kim, J. J., Moon, H.I., Ro, H.M., Kim, E. H., Seo, S.H., Kang, E.Y., Ahn, J.K., Chung, I.M. Isoflavones in Korean soybeans differing in seed coat and cotyledon color. J. Food Compos. Anal. 2010, 23, 160−165. [8] Jeng, T.L., Shih, Y.J., Wu, M.T., Sung, J.M. Comparisons of flavonoids and anti-oxidative activities in seed coat, embryonic axis and cotyledon of black soybeans. Food Chem. 2010, 123, 1112−1116. [9] Luthria, D.L., Biswas, R., Natarajan, S. 2007. Comparison of extraction solvents and techniques used for the assay of isoflavones from soybean. Food Chemistry, 105(1), 325-333. [10] Kelebek, H. LC-DAD-ESI-MS/MS characterization of phenolic constituents in Turkish black tea: Effect of infusion time and temperature. Food Chem 204: 227–238 (2016). [11] Kelebek, H., Jourdes, M., Selli, S., and Teissedre P-L, Comparative evaluation of the phenolic content and antioxidant capacity of sun-dried raisins. J Sci Food Agric 93(12): 2963–72 (2013).

Year 2018, Volume 1, Issue 2, 70 - 78, 31.12.2018

Abstract

References

  • [1] Lee J.H., Jeon J.Y., Kim S.G., Kim S.H., Chun T., Imm J.Y., Comparative analyses of total phenols, flavonoids, saponins and antioxidant activity in yellow soy beans and mung beans. Int. J. Food Sci. Tech., 2011, 46, 2513–2519. [2] Nagata C., Takatsuka N., Inaba S., Kawakawi N., Shimizu H., Effect of soymilk consumption on serum estrogen concentrations in premenopausal Japanese women. J. Natl. Cancer Inst., 1998, 90, 1830–1835. [3] Dueñas, M., Hernández, T., Robredo, S., Lamparski, G., Estrella, I., Muñoz, R. (2012). Bioactive Phenolic Compounds of Soybean (Glycine max cv. Merit): Modifi cations by Different Microbiological Fermentations. Pol. J. Food Nutr. Sci., 62, 4, 241-250. [4] Wang, H. J., Murphy, P. A. Isoflavone content in commercial soybean foods. J. Agric. Food Chem. 1994, 42, 1666−1673. [5] Li, Y., Kong, D., Bao, B., Ahmad, A., Sarkar, F. H. Induction of cancer cell death by isoflavone: the role of multiple signaling pathways. Nutrition 2011, 3, 877−896. [6] Albertazzi, P., Purdie, D. W. The nature and utility of the phytoestrogens: a review of the evidence. Maturitas 2002, 42, 173−185. [7] Lee, S.J., Seguin, P., Kim, J. J., Moon, H.I., Ro, H.M., Kim, E. H., Seo, S.H., Kang, E.Y., Ahn, J.K., Chung, I.M. Isoflavones in Korean soybeans differing in seed coat and cotyledon color. J. Food Compos. Anal. 2010, 23, 160−165. [8] Jeng, T.L., Shih, Y.J., Wu, M.T., Sung, J.M. Comparisons of flavonoids and anti-oxidative activities in seed coat, embryonic axis and cotyledon of black soybeans. Food Chem. 2010, 123, 1112−1116. [9] Luthria, D.L., Biswas, R., Natarajan, S. 2007. Comparison of extraction solvents and techniques used for the assay of isoflavones from soybean. Food Chemistry, 105(1), 325-333. [10] Kelebek, H. LC-DAD-ESI-MS/MS characterization of phenolic constituents in Turkish black tea: Effect of infusion time and temperature. Food Chem 204: 227–238 (2016). [11] Kelebek, H., Jourdes, M., Selli, S., and Teissedre P-L, Comparative evaluation of the phenolic content and antioxidant capacity of sun-dried raisins. J Sci Food Agric 93(12): 2963–72 (2013).

Details

Primary Language English
Subjects Engineering
Journal Section Articles
Authors

Haşim KELEBEK (Primary Author)
ADANA BİLİM VE TEKNOLOJİ ÜNİVERSİTESİ
Türkiye


Serkan SELLİ
0000-0003-0450-2668

Publication Date December 31, 2018
Published in Issue Year 2018, Volume 1, Issue 2

Cite

Bibtex @research article { artibilim491851, journal = {Artıbilim: Adana Bilim ve Teknoloji Üniversitesi Fen Bilimleri Dergisi}, address = {Gültepe Mahallesi, Çatalan Caddesi No:201/5 01250 Sarıçam/ADANA}, publisher = {Adana Alparslan Türkeş Bilim ve Teknoloji Üniversitesi}, year = {2018}, volume = {1}, number = {2}, pages = {70 - 78}, title = {LC-DAD-ESI-MS/MS characterization of soybean phenolics extracted with various solvents}, key = {cite}, author = {Kelebek, Haşim and Selli, Serkan} }
APA Kelebek, H. & Selli, S. (2018). LC-DAD-ESI-MS/MS characterization of soybean phenolics extracted with various solvents . Artıbilim: Adana Bilim ve Teknoloji Üniversitesi Fen Bilimleri Dergisi , 1 (2) , 70-78 . Retrieved from https://dergipark.org.tr/en/pub/artibilim/issue/41954/491851
MLA Kelebek, H. , Selli, S. "LC-DAD-ESI-MS/MS characterization of soybean phenolics extracted with various solvents" . Artıbilim: Adana Bilim ve Teknoloji Üniversitesi Fen Bilimleri Dergisi 1 (2018 ): 70-78 <https://dergipark.org.tr/en/pub/artibilim/issue/41954/491851>
Chicago Kelebek, H. , Selli, S. "LC-DAD-ESI-MS/MS characterization of soybean phenolics extracted with various solvents". Artıbilim: Adana Bilim ve Teknoloji Üniversitesi Fen Bilimleri Dergisi 1 (2018 ): 70-78
RIS TY - JOUR T1 - LC-DAD-ESI-MS/MS characterization of soybean phenolics extracted with various solvents AU - Haşim Kelebek , Serkan Selli Y1 - 2018 PY - 2018 N1 - DO - T2 - Artıbilim: Adana Bilim ve Teknoloji Üniversitesi Fen Bilimleri Dergisi JF - Journal JO - JOR SP - 70 EP - 78 VL - 1 IS - 2 SN - - M3 - UR - Y2 - 2018 ER -
EndNote %0 Artıbilim: Adana Bilim ve Teknoloji Üniversitesi Fen Bilimleri Dergisi LC-DAD-ESI-MS/MS characterization of soybean phenolics extracted with various solvents %A Haşim Kelebek , Serkan Selli %T LC-DAD-ESI-MS/MS characterization of soybean phenolics extracted with various solvents %D 2018 %J Artıbilim: Adana Bilim ve Teknoloji Üniversitesi Fen Bilimleri Dergisi %P - %V 1 %N 2 %R %U
ISNAD Kelebek, Haşim , Selli, Serkan . "LC-DAD-ESI-MS/MS characterization of soybean phenolics extracted with various solvents". Artıbilim: Adana Bilim ve Teknoloji Üniversitesi Fen Bilimleri Dergisi 1 / 2 (December 2018): 70-78 .
AMA Kelebek H. , Selli S. LC-DAD-ESI-MS/MS characterization of soybean phenolics extracted with various solvents. Artıbilim: Adana Bilim ve Teknoloji Üniversitesi Fen Bilimleri Dergisi. 2018; 1(2): 70-78.
Vancouver Kelebek H. , Selli S. LC-DAD-ESI-MS/MS characterization of soybean phenolics extracted with various solvents. Artıbilim: Adana Bilim ve Teknoloji Üniversitesi Fen Bilimleri Dergisi. 2018; 1(2): 70-78.
IEEE H. Kelebek and S. Selli , "LC-DAD-ESI-MS/MS characterization of soybean phenolics extracted with various solvents", Artıbilim: Adana Bilim ve Teknoloji Üniversitesi Fen Bilimleri Dergisi, vol. 1, no. 2, pp. 70-78, Dec. 2018