Phenolics are broadly distributed in the plant kingdom and the most abundant secondary metabolites of plants. Over the past few years, preparation of phenolic extracts from different plants, purification of phenolic compounds and their identification have become a major area of health- and medical-related research. In this study, the effect of extraction solvents (methanol, ethanol, acetonitrile and acetone), on total phenolic content, antioxidant activity, and the composition of the phenolic compounds in soybean extracts were studied. The antioxidant activity was determined using two different methods: DPPH and ABTS assays. Liquid chromatography coupled to diode array detection and electrospray ionisation tandem mass spectrometry (LC-DAD-ESI-MS/MS) was used for identification and quantification of phenolic compounds. All the parameters analyzed were found to be affected by the extraction method, especially by the solvent used, and the best results were obtained in the methanolic extract. The methanolic and ethanolic extracts exhibited strong antioxidant activity. Genistein-7-diglucoside, daidzin, genistin, 4’ 7 dihidroxiflavan, daidzein, and genistein were found as major isoflavonoids in all extracts.
Primary Language | English |
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Subjects | Engineering |
Journal Section | Articles |
Authors |
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Publication Date | December 31, 2018 |
Published in Issue | Year 2018, Volume 1, Issue 2 |
Bibtex | @research article { artibilim491851, journal = {Artıbilim: Adana Bilim ve Teknoloji Üniversitesi Fen Bilimleri Dergisi}, address = {Gültepe Mahallesi, Çatalan Caddesi No:201/5 01250 Sarıçam/ADANA}, publisher = {Adana Alparslan Türkeş Bilim ve Teknoloji Üniversitesi}, year = {2018}, volume = {1}, number = {2}, pages = {70 - 78}, title = {LC-DAD-ESI-MS/MS characterization of soybean phenolics extracted with various solvents}, key = {cite}, author = {Kelebek, Haşim and Selli, Serkan} } |
APA | Kelebek, H. & Selli, S. (2018). LC-DAD-ESI-MS/MS characterization of soybean phenolics extracted with various solvents . Artıbilim: Adana Bilim ve Teknoloji Üniversitesi Fen Bilimleri Dergisi , 1 (2) , 70-78 . Retrieved from https://dergipark.org.tr/en/pub/artibilim/issue/41954/491851 |
MLA | Kelebek, H. , Selli, S. "LC-DAD-ESI-MS/MS characterization of soybean phenolics extracted with various solvents" . Artıbilim: Adana Bilim ve Teknoloji Üniversitesi Fen Bilimleri Dergisi 1 (2018 ): 70-78 <https://dergipark.org.tr/en/pub/artibilim/issue/41954/491851> |
Chicago | Kelebek, H. , Selli, S. "LC-DAD-ESI-MS/MS characterization of soybean phenolics extracted with various solvents". Artıbilim: Adana Bilim ve Teknoloji Üniversitesi Fen Bilimleri Dergisi 1 (2018 ): 70-78 |
RIS | TY - JOUR T1 - LC-DAD-ESI-MS/MS characterization of soybean phenolics extracted with various solvents AU - Haşim Kelebek , Serkan Selli Y1 - 2018 PY - 2018 N1 - DO - T2 - Artıbilim: Adana Bilim ve Teknoloji Üniversitesi Fen Bilimleri Dergisi JF - Journal JO - JOR SP - 70 EP - 78 VL - 1 IS - 2 SN - - M3 - UR - Y2 - 2018 ER - |
EndNote | %0 Artıbilim: Adana Bilim ve Teknoloji Üniversitesi Fen Bilimleri Dergisi LC-DAD-ESI-MS/MS characterization of soybean phenolics extracted with various solvents %A Haşim Kelebek , Serkan Selli %T LC-DAD-ESI-MS/MS characterization of soybean phenolics extracted with various solvents %D 2018 %J Artıbilim: Adana Bilim ve Teknoloji Üniversitesi Fen Bilimleri Dergisi %P - %V 1 %N 2 %R %U |
ISNAD | Kelebek, Haşim , Selli, Serkan . "LC-DAD-ESI-MS/MS characterization of soybean phenolics extracted with various solvents". Artıbilim: Adana Bilim ve Teknoloji Üniversitesi Fen Bilimleri Dergisi 1 / 2 (December 2018): 70-78 . |
AMA | Kelebek H. , Selli S. LC-DAD-ESI-MS/MS characterization of soybean phenolics extracted with various solvents. Artıbilim: Adana Bilim ve Teknoloji Üniversitesi Fen Bilimleri Dergisi. 2018; 1(2): 70-78. |
Vancouver | Kelebek H. , Selli S. LC-DAD-ESI-MS/MS characterization of soybean phenolics extracted with various solvents. Artıbilim: Adana Bilim ve Teknoloji Üniversitesi Fen Bilimleri Dergisi. 2018; 1(2): 70-78. |
IEEE | H. Kelebek and S. Selli , "LC-DAD-ESI-MS/MS characterization of soybean phenolics extracted with various solvents", Artıbilim: Adana Bilim ve Teknoloji Üniversitesi Fen Bilimleri Dergisi, vol. 1, no. 2, pp. 70-78, Dec. 2018 |