Türkiye’nin Artvin ve Giresun bölgelerindeki Kantaron (Hypericum montbretii ve Hypericum bupleuroides) ve Karaçalı (Paliurus spina-christi Mill) bitkilerinin antioksidan ve antimikrobiyal aktivitelerinin karşılaştırılması

Abstract

Özet

Tıbbi ve aromatik bitkilerden olan Kantaron (Hypericum montbretii ve Hypericum bupleuroides) ve karaçalı (Paliurus spina-christi Mill) bitkilerinin yaprak, çiçek ve kök gibi kısımlarından oluşan 13 farklı örneğin antioksidan aktivitesi DPPH, FRAP ve CUPRAC yöntemleri kulanılarak incelenmiştir. Aynı zamanda bitki ekstraktların toplam fenolik ve toplam flavonoid miktarları da ölçülmüş ve antimikrobiyal özellikleri de belirlenmiştir. Antioksidan aktivite sonuçlarına göre tüm analizler içinde (toplam polifenol miktar analizi hariç) en iyi aktivite Kantaron (Hypericum montbretii) bitkisinin kuru yaprağında olduğu bulunmuştur. Toplam polifenol analizine göre ise en yüksek aktivitenin 22.485±0.656 mg GAE /g değeri ile Kantaron’un (Hypericum montbretii) çiçek kısmına ait olduğu görülmüştür. Antimikrobiyal analizler ise disk düfüzyon yöntemi kullanılarak yapılmış ve bitki ekstraktlarının 12 tane test mikrorganizması üzerine etkisi incelenmiştir. Bu sonuçlara göre bitkilerden çoğunun bakterilere ve mantar suşlarına karşı iyi derecede aktivite gösterdiği gözlenmiştir.

Keywords

• Kantaron,Karaçalı,DPPH,FRAP,antioksidan

Comparison of antioxidant and antimicrobial activities of plant extracts Centaury (Hypericum montbretii and Hypericum bupleuroides) and Blackthorn (Paliurus spina-christi Mill) in Artvin, Giresun region of Turkey

Abstract

Abstract

Antioxidant activity 13 different samples of medicinal and aromatic plants of Centaury (Hypericum montbretii), Centaury (Hypericum bupleuroides), Blackthorn (Paliurus spina-christi Mill), including leaf, flower and stem, was investigated using DPPH, FRAP and CUPRAC methods. Total flavonoid, total phenolic amount and the antibacterial features of extracts from these herbs were also determined. According to the obtained of antioxidant datas, except for the result of total polyphenol assay, the dried leaves of Centuary (Hypericum montbretii) had the best antioxidant property that was realized in all results. Accordingly test of total polyphenol, the content of yellow flower of Centuary (Hypericum montbretii) herb was measured as of 22.48±0.66 mg GAE /g dry herb sample and so this herb had the highest total phenolic content. Antimicrobial activity tests were carried out using disc diffusion methods with 12 microbial species and the most of them displayed good-moderate antimicrobial and antifungal activity.

Keywords

• Centaury,Blackthorn,DPPH,FRAP,antioxidant

References

1. Al-Mamary M, Al-Meeri A, Al-Habori M (2002) Antioxidantactivities and total phenolics of different types of honey. Nutr Res 22:1041-047.
2. Amelia A, Almeida P, Farah A, Silva DAM, Nunan EA. and Gloria BA (2006) Antibacterial Activity of Coffee Extracts and Selected Coffee Chemical Compounds against Enterobacteria. J Agr Food Chem 54:8738-8743.
3. Apak R, Güçlü K, Özyürek M, Karademir SE (2004) Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. J Agr Food Chem 52:7970-7981.
4. Benzie IF, Szeto YT (1999) Total antioxidant capacity of teas by the ferric reducing/antioxidant power assay. J Agr Food Chem 47:633-636.
5. Brantner A, Males Z, Pepeljnjak S, Antolic A (1996) Antimicrobial activity of Paliurus spina-christi Mill. (Christ's thorn). J Ethnopharmacol 52:119-122.
6. Brantner AH, Males Z (1999) Quality assessment of Paliurus spina-christi extracts. J Ethnopharmacol 66:175-179.
7. Chang CC, Yang MH, Wen HM, Chern JC (2002) Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal 10:178-182.
8. Côté J, Caillet S, Doyon G, Sylvain JF, Lacroix M (2010) Bioactive compounds in cranberries and their biological properties. Crit Rev Food Sci 50:666-679.

9. Cowan MM (1999) Plant products as antimicrobial agents. Clin Microbial Rev 12:564-582.
10. Crockett SL, Robson NK (2001) Taxonomy and chemotaxonomy of the genus Hypericum. Med Aromat Plant Sci Biotechnol (Special Issue 1):1-13.
11. Dall'Agnol R, Ferraz A., Bernardi A, Albring D, Nör C, Sarmento L, Lamb L, Hass M, von Poser G, Schapoval EES (2003) Antimicrobial activity of some Hypericum species. Phytomedicine 10:511-516.
12. Das K, Tiwari R, Shrivastava D (2010) Techniques for evaluation of medicinal plant products as antimicrobial agent: Current methods and future trends. J Med Plants Res 4:104-111.
13. Demiray S, Pintado M, Castro P (2009) Evaluation of phenolic profiles and antioxidant activities of Turkish medicinal plants: Tilia argentea, Crataegi folium leaves and Polygonum bistorta roots. World Acad Sci Eng Technol 54:312-317.
14. Devasagayam T, Tilak J, Boloor K, Sane KS, Ghaskadbi SS, Lele R (2004) Free radicals and antioxidants in human health: current status and future prospects. J Assoc Physician I 52:794-804.
15. Farnsworth NR, Akerele O, Bingel AS, Soejarto DD, Guo Z (1985) Medicinal plants in therapy. B. World Health Organ 636:965-981.
16. Fathi H, Ebrahimzadeh MA (2013) Antioxidant and free radical scavenging activities of Hypericum perforatum L.(st. John’s wort). Int J Forest Soil and Erosion (IJFSE) 3:68-72.
17. Finkel T, Holbrook NJ (2000) Oxidants, oxidative stress and the biology of ageing. Nature 408:239-247.
18. Ilcim A, Digak M (1998) The investigation of antimicrobial effect of some plant extract. Turk J Biol 22:119-126
19. Kähkönen MP, Hopia AI, Vuorela HJ, Rauha JP, Pihlaja K, Kujala TS, Heinonen M (1999) Antioxidant activity of plant extracts containing phenolic compounds. J Agr Food Chem 47:3954-3962.
20. Katalinic V, Milos M, Kulisic T, Jukic M (2006) Screening of 70 medicinal plant extracts for antioxidant capacity and total phenols. Food Chem 94:550-557.
21. Kırca A, Bilişli A, Demirel NN, Turhan H, Arslan E (2008) Determination of antioxidant activity of some medicinal and aromatic flora of Çanakkale, Master Thesis.
22. Klaunig JE, Kamendulis LM (2004) The role of oxidative stress in carcinogenesis. Annu. Rev. Pharmacol Toxicol 44:239-267.
23. Murray P R, Baron EJ, Pfaller MA, Tenover FC, Yolke RH (1995) Manual of clinical microbiology (7th ed.). pp. 1773, Washington, DC: ASM.
24. Organization, WH (2001) WHO global strategy for containment of antimicrobial resistance.
25. Ozer H, Sokmen M, Gulluce M, Adıguzel A, Sahin F, Sokmen A, Kılıc H and Barış O (2007) Chemical Composition and Antimicrobial and Antioxidant Activities of the Essential Oil and Methanol Extract of Hippomarathrum microcarpum (Bieb.) from Turkey. J Agr Food Chem 55:937-942.
26. Pokorny J, Yanishlieva N, Gordon M (2001) Antioxidants in Food, CRC Pres, USA.
27. Reichling J, Weseler A, Saller R (2001) A current review of the antimicrobial activity of Hypericum perforatum L. Pharmacopsychiatry 34:116-118.
28. Robards K, Prenzler PD, Tucker G, Swatsitang P, Glover W (1999) Phenolic compounds and their role in oxidative processes in fruits. Food Chem 66:401-436.
29. Sakar M, Tamer A (1990) Antimicrobial activity of different extracts from some Hypericum species. Fitoterapia 61:464-466.
30. Slinkard K, Singleton VL (1977) Total phenol analysis: Automation and comparison with manual methods. Am J Enol Viticult 28:49-55.
31. Vaya J, Belinky PA and Aviram M (1997) Antioxidant constituents from licorice roots: Isolation, structure elucidation and antioxidative capacity toward LDL oxidation. Free Radical Bio Med 23:302-313.