Comparison of HIF-1⍺ and EPO Levels Before and After Exercise in Athletes Acclimatized to Low-Middle Altitude

Abstract

This study was conducted to examine the effect of acute exercise on HIF-1⍺ and EPO serum levels in well-trained male skiers adapted to low-medium altitude. The study was conducted with 15 male skiers with an average age of 20.33±1.91 years, average height of 170.93±10.62cm, and average body mass of 62.80±11.00kg. Bruce Treadmill test was performed to determine the participants' maximum oxygen consumption and to interpret their aerobic power levels. Determination of levels of selected blood cells, HIF-1α and EPO; Blood was taken from the participants at an altitude of 1000m, an exercise test was performed at the ski resort at an altitude of 2000m on the same day, blood was taken again 2 hours later, and blood was taken again the next morning, three times. Skiers who continued their normal training cycles were subjected to a single-repetition exercise protocol and the levels of relevant markers were determined with before and after blood tests. As a result, the highest level in selected blood parameters was reached at the last test (after 24 hours) (except WBC). It was observed that the difference between the tests was significant (p<0.05) in WBC, RBC, HGB, HCT parameters (except PLT). While the difference between the tests at the HIF-1α level was not significant (p>0.05), it was determined to be significant at the EPO level (p<0.05). In such studies, it is thought that sedentary-athlete comparison, chronic exercise tests and temporal follow-up spread over wider hours/days will contribute to the literature with more qualified results.

Keywords

• Hif-1 Alpha
• Epo
• Altitude
• Hypoxia
• Aerobic Exercise

Düşük-Orta İrtifaya Aklimatize Sporcularda Egzersiz Öncesi ve Sonrası HIF-1⍺ ve EPO Düzeylerinin Karşılaştırılması

Öz

Bu çalışma, düşük-orta irtifaya uyum sağlamış, iyi antrenmanlı erkek kayakçılarda akut egzersizin HIF-1⍺ ve EPO serum düzeylerine etkisini incelemek amacıyla yapıldı. Çalışma, yaş ortalaması 20.33±1.91yıl, boy ortalaması 170.93±10.62cm, vücut kütle ortalaması 62.80±11.00kg olan 15 erkek kayakçı ile yapıldı. Katılımcıların, VO2max için Bruce treadmill testi yapıldı. Seçilmiş kan hücreleri, HIF-1α ve EPO düzeylerinin belirlenmesi; 1000m rakım düzeyinde katılımcılardan kan alımı, aynı gün içerisinde 2000m rakımlı kayak merkezinde egzersiz testi ve 2 saat sonrasında tekrar kan alımı, ertesi sabah yine kan alımı şeklinde üç tekrarlı olarak yapıldı. Normal antrenman döngülerine devam eden kayakçılar, tek tekrarlı egzersiz protokolüne tabi tutularak, öncesi-sonrası yapılan kan testleriyle, ilgili markerlerin düzeyi belirlendi. Sonuç olarak, seçilmiş kan parametrelerinde en yüksek düzeye son testte (24 saat sonra) ulaşıldı (WBC hariç). WBC, RBC, HGB, HCT parametrelerinde testler arası farkın anlamlı olduğu (p<0.05) görüldü (PLT hariç). HIF-1α düzeyinde testler arasındaki farkın anlamlı olmadığı (p>0.05) görülürken, EPO düzeyinde anlamlı olduğu (p<0.05) belirlendi. Bu tip çalışmalarda, sedanter-sporcu karşılaştırması, kronik egzersiz testlerinin yapılması ve zamansal takibin daha geniş saatlere/günlere yayılmasının daha nitelikli sonuçlarla literatüre katkı sağlayacağı düşünülmektedir.

Anahtar Kelimeler

• Hif-1 Alfa
• Epo
• İrtifa
• Hipoksi
• Aerobik Egzersiz

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