THE EFFECT OF MICRODIETS ON SURVIVAL AND GROWTH RATE OF GILTHEAD SEA BREAM (Sparus aurata L, 1758) LARVAE GROWTH IN DIFFERENT SALINITY LEVEL

Abstract

In this study, gilthead seabream larvae were fed with microdiets having added digestive enzymes (porcine, pancreatin enzymes) in between 16^th-30^th day after yolk resorption and their growth, weight and survival rate were determined. After yolk resorption (the 4^th day) until the 15^th day, larvae being fed with only rotifer (B. pilicatilis), between 16^th-20^th days larvae were fed with rotifer and microdiets together. Between the 21^th and 30^th days larvae were fed only with microdiets. By taking into consideration the salinity desire of larvae in constituting trial groups, larvae were fed with microdiets in both   25ppt and ‰40. The growth was founded significant in all of trial groups that were fed with live food (in ‰40 and 25‰ salinity) according to those fed with microdiet (P < 0.05). The growth in ‰25 salinity was founded significant both for live food and microdiet in ‰40 salinity (P < 0.05). The highest survival rate was founded significant by feeding live food in 25‰ salinity (P<0.05).                

Keywords

• Gilthead seabream,Sparus aurata,Larval feeding,Microdiet,Enzymes

FARKLI TUZLULUK SEVİYELERİNDE YETİŞTİRİLEN ÇİPURA (Sparus aurata L, 1758) LARVALARININ BÜYÜME VE YAŞAM ORANINA MİKRO DİYET YEMLERİN ETKİSİ

Öz

Araştırmada, deniz balıkları larva yetiştiriciliğinde yoğun olarak kullanılan Brachionus spp., Artemia spp. gibi canlı yemlere alternatif, enzim ilaveli mikro diyet yemlerin, Sparus aurata türünün erken larval döneminde kullanımının etkisi araştırılmıştır. Denemede çipura larvaları, besin kesesi çekildikten sonra 15. güne kadar Brachionus spp. ile beslenmiş larvalar, 16. gün ile 30. günler arasında enzim ilave edilmiş mikro diyet yemlerle beslenmesi yapılarak toplam boy uzaması, ağırlık artışı ve yaşama oranları tespit edilmiştir. Canlı yem grubunda larvalar deneme süresince sadece Brachionus spp. ile beslenmiştir. Deneme gruplarının oluşturulmasında larvaların tuzluluk isteği göz önünde tutularak hem ‰40, hem de ‰25 tuzlulukta larvalar mikro diyet yemle beslenmiştir. Deneme süresince ‰40 ve ‰25 tuzlulukta canlı yemle beslenen gruplarında larval büyümenin (ağırlıkça ve boyca) mikro diyet yemle beslenen gruplara göre istatiksel olarak daha iyi olduğu belirlenmiştir (P<0,05). Tuzluluğun ‰25 olduğu grupta büyüme, ‰40 tuzluluktaki hem canlı yem, hem de mikro diyet yemle beslenen gruplara göre istatistiksel olarak daha iyi olduğu belirlenmiştir (P<0,05). En yüksek yaşama oranı, ‰25 tuzlulukta canlı yem ile beslenen grupta gözlenmiştir. 

Anahtar Kelimeler

• Çipura,Sparus aurata,Larva besleme,Mikro diyet yem,Yaşam oranı,Büyüme

References

1. Curnow, J., King J., Bosmans, J., & Kolkovski, S. (2006). The effect of reduced Artemia and rotifer use facilitated by a new microdiet in the rearing of barramundi Lates calcarifer (BLOCH) larvae. Aquaculture, 257 (1-4), 204-213. doi:10.1016/j.aquaculture.2006.02.073
2. Düzgüneş, O., Kesici, T., & Gürbüz, F. (1993). İstatistik Metodları II, Ankara Üniv. Ziraat Fak., Yayın No: 1291, Ders Kitabı: 391, Ankara Üniv., Ziraat Fak., Baskı Ofset Ünitesi, 218s.
3. Fernández-Díaz, C., Pascual, E., & Yúfera, M. (1994). Feeding behaviour and prey size selection of gilthead seabream, Sparus aurata, larvae fed on inert and live food. Marine Biology, 118, 323-328. doi: 10.1007/BF00349800
4. Fernández-Díaz, C., & Yúfera, M. (1995). Capacity of gilthead seabream, Sparus aurata L., larvae to break down dietary microcapsules. Aquaculture, 134 (3-4), 269-278. doi:10.1016/0044-8486(95)00058-A
5. Fernández-Díaz, C., & Yúfera, M. (1997). Detecting growth in gilthead seabream, Sparus aurata L., larvae fed microcapsules. Aquaculture, 153 (1-2), 93-102. doi:10.1016/S0044-8486(97)00017-3
6. Howell, B. (1996). Closer Focus on Flatfish Culture. Fish Farmer Int. File, 10(5), 27-30.
7. Kolkovski, S., Tandler, A., Kissil, Wm. G. & Gertler, A. (1993a). The effect of dietary exogenous digestive enzymes on ingestion, assimilation, growth and survival of gilthead sea bream (Sparus aurata, Sparidae, Linnaeus) larvae. Fish Phys. and Biochem., 12(3), 203-209. doi: 10.1007/BF00004368
8. Kolkovski, S., Tandler, A., Kissil, & Wm. G. (1993b). The effect of dietary enzymes with age on protein and lipid assimilation and deposition in Sparus aurata larvae. In: Fish Nutrition in Practice (ed: Kaushik, S., Lucquet, P.,). 569-578, 4th International Symposium on Fish Nutrition and Feeding. Biarritz. 24-27 June 1991. INRA, Paris.

9. Kolkovski, S., Arieli, A., & Tandler, A. (1997a). Visual and chemical cues stimuli microdiet ingestion in sea bream larvae. Aquaculture International, 5(6), 527-536. doi:10.1023/A:1018305416501
10. Kolkovski, S., Kowen, W. & Tandler, A. (1997b). The mode of action of Artemia in enhancing utilisation of microdiet by gilthead seabream Sparus aurata larvae. Aquaculture, 155(1-4), 193-205. doi:10.1016/S0044-8486(97)00117-8
11. Kolkovski, S., Tandler, A., & Izquierdo, M.S. (1997c). Effects of live food and dietary digestive enzymes on the efficiency of microdiets for seabass (Dicentrarchus labrax) larvae. Aquaculture, 148(6), 313-322. doi:10.1016/S0044-8486(96)01366-X
12. Kolkovski, S. 2001. Digestive enzymes in fish larvae and juveniles—implications and applications to formulated diets. Aquaculture, 200 (1-2), 181-201 doi:10.1016/S0044-8486(01)00700-1
13. Kowen, W.M., Kolkovski, S., Tandler, A., Kissil, Wm. G., & Sklan, D. (1993). The effect of dietary lecithin and lipase, as a function of age, on n-9 fatty acid incorporation in the tissue lipids of Sparus aurata larvae. Fish Phys. and Biochem., 10(5), 357-364. doi:10.1007/BF00004502.
14. Kowen, W.M., Parra, G., Kolkovski, S., & Tandler, A. (1998). The effect of dietary phosphatidylcholine and its constituent fatty acids microdiet ingestion and fatty acid absorption rate in gilthead sea bream, Sparus aurata, larvae. Aquaculture Nutrition, 4(1), 39-45 doi: 10.1046/j.1365-2095.1998.00101.x
15. Lubzens, E., Minkoff, G., Barr, Y., & Zmora, O. (1997). Mariculture in Israel: Past achievements and future directions in raising rotifers as food for marine fish larvae. Hydrobiologia. 358(1), 13-19. doi: 10.1023/A:1003117610203
16. Morris, P. (1997). Nutritional needs of bass and bream. Fish Farmer Int. File., 11(6), 28-31.
17. Sáenz De Rodrıgáñez, M.A., Gander, B., Alaiz, M., & Moyano, F.J. (2011), Physico-chemical characterization and in vitro digestibility of commercial feeds used in weaning of marine fish. Aquaculture Nutrition, 17, 429-440. doi: 10.1111/j.1365-2095.2010.00820.x
18. Salhi, M., Izquierdo, M.S., Hernande-Cruz, C.M., Socorro, J., & Fernandez-Palacios, H. (1997). The improved incorporation of polyunsaturated fatty acids and changes in liver structure in larval gilthead sea bream fed on microdiets. Journal Fish Biol., 51(5) 869-879. doi: 10.1111/j.1095-8649.1997.tb01526.x
19. Salhi, M., & Bessonart, M. (2011). Growth, survival and fatty acid composition of Rhamdia quelen(Quoy and Gaimard, 1824) larvae fed on artificial diet alone or in combination with Artemia nauplii. Aquaculture Research, 44, 41-49. doi: 10.1111/j.1365-2109.2011.03004.x
20. Tandler, A., & Kolkovski, S. (1992). Rates of ingestion and digestibility as limiting factors in the successful application of microdiets in gilthead seabream Sparus aurata larvae. Isr. J. Aquacult. Bamidgeh, 44(4), 128-129.
21. Watanabe, T. (1992). Nutrition and Growth. In: Intensive Fish Farming. (Ed: Shepherd, J.C., Bromage, N.R.,), London, 154-197p.
22. Watanabe, W.O., Alam, M.S., Ostrowskia, A.D., Montgomerya, F.A., Gabela, J.E., Morris, J.A., & Seaton, P.J. (2016). Live prey enrichment and artificial microdiets for larviculture ofAtlantic red porgy Pagrus pagrus. Aquaculture Reports, 3, 93-107
23. Yúfera, M., Fernández-Díaz, C., & Pascual, E. (1995). Feeding rates of gilthead seabream (Sparus aurata), larvae on microcapsules. Aquaculture, 134, (3-4), 257-268. doi:10.1016/0044-8486(95)00035-Z
24. Yúfera, M., Sarasquete, M.C., & Fernández-Díaz, C. (1996). Testing protein-walled microcapsules for the rearing of first-feeding gilthead sea bream (Sparus aurata l.) larvae. Mar. Freshwater Res., 47, 211-216. doi:10.1071/MF9960211
25. Yúfera, M., Pascual, E., & Fernández-Díaz, C. (1999). A highly efficient microencapsulated food for rearing early larvae of marine fish. Aquaculture, 177(1-4), 249-256. doi:10.1016/S0044-8486(99)00088-5