Oksitosin ile İndüklenen Doğumu Olan Hastalarda Yeni Doğanların Kordon Kanındaki Dinamik Tiyol/Disülfid Homeostazisi ve İskemi-Modifiye-Albümin Düzeylerinin Değerlendirilmesi

Öz

Bu çalışmanın amacı oksitosine bağlı doğum eylemi olan hastalarda yenidoğanların kordon kanındaki hem dinamik tiyol-disülfid hem de iskemi modifiye albümin (IMA) düzeylerini değerlendirmektir. Oksitosin ile tıbbi doğum indüksiyonu yapılan toplam 30 hamile kadın, doğumu herhangi bir indüksiyon olmaksızın kendiliğinden ilerleyen 30 kadınla karşılaştırıldı. Dinamik tiyol-disülfid dengesi, Erel ve arkadaşları tarafından geliştirilen yeni kolorimetrik yöntemle belirlendi. IMA seviyeleri Bar-Or ve arkadaşları tarafından geliştirilen albümin kobalt bağlanma testi kullanılarak analiz edildi. Oksitosin ile indüklenen grupta kontrollere göre TAS ve OSI seviyelerinin daha yüksek ve TOS seviyelerinin daha düşük olduğu bulundu. Oksitosin ile indüklenen grupta kontrol grubuna göre native ve toplam tiyol seviyeleri daha düşük, disülfid seviyeleri ise daha yüksek bulundu. IMA seviyeleri hasta ve kontrol grupları arasında anlamlı olarak farklı bulundu. Oksitosin ile indüklenen hastaların yenidoğanlarında disülfid oluşumunun antioksidan kapasitede azalmaya yol açtığını düşünüyoruz . Dinamik tiyol-disülfid homeostazı, oksitosin ile indüklenen hastaların bebeklerinde oksidatif stresin izlenmesine ve oksidatif strese bağlı ortaya çıkabilecek hastalıkların takibine katkı sağlayabilir.

Anahtar Kelimeler

• Newborn
• Oxidative stress
• Ischemia-modified albumin
• Thiol-disulfide homeostasis
• Oxytocin induction.

Evaluation of Dynamic Thiol /Disulfide Homeostasis and Ischemia- Modified-Albumin Levels in Cord Blood of Newborns to Patients with Oxytocin-Induced Labor

Abstract

The aim of this study is to evaluate both dynamic thiol-disulfide and ischemia modified albumin (IMA) levels in cord blood of newborns to patients with oxytocin-induced labor. A total of 30 pregnant women who underwent medical labor induction with oxytocin were compared with 30 women whose labor progressed spontaneously without any kind of induction. The dynamic thiol-disulfide balance was determined by the new colorimetric method developed by Erel et al. IMA levels were analyzed by using albumin cobalt binding test developed by Bar-Or et al. It was found that TAS and OSI levels were higher and TOS levels were lower in the oxytocin-induced group compared to the controls. Native and total thiol levels were found lower while disulfide levels were found higher oxytocin-induced group respect to the control. IMA levels were found significantly different between the patient and control subjects. We indicate that formation of disulfide leads to decrease antioxidant capacity in newborns of oxytocin-induced patients. Dynamic thiol-disulfide homeostasis may contribute to the monitoring of oxidative stress in infants of oxytocin-induced patients and the follow-up of diseases that may arise due to oxidative stress.

Keywords

• Newborn
• Oxidative stress
• Ischemia-modified albumin
• Thiol-disulfide homeostasis
• Oxytocin induction.

Kaynakça

1. Alchalabi, H. A., Obeidat, B. R., Jallad, M. F., & Khader, Y. S. (2006). Induction of labor and perinatal outcome: the impact of the amniotic fluid index. Eur J Obstet Gynecol Reprod Biol, 129(2), 124-127. doi: 10.1016/j.ejogrb.2005.10.039
2. Bar-Or, D., Curtis, G., Rao, N., Bampos, N., & Lau, E. (2001). Characterization of the Co(2+) and Ni(2+) binding amino-acid residues of the N-terminus of human albumin. An insight into the mechanism of a new assay for myocardial ischemia. Eur J Biochem, 268(1), 42-47. doi: 10.1046/j.1432-1327.2001.01846.x
3. Cakirca, G., C. M., Erdal H, Neselioglu S, Erel O, Basarali MK, Cakirca TD. (2018). Investigation of thiol/disulfide homeostasis in familial mediterranean fever patients. Clin Anal Med, 9(3), 231-234. doi: 10.4328/JCAM.5789
4. Demirtas, M.S., Erdal H . Evaluation of thiol-disulfide homeostasis and oxidative stress parameters in newborns receiving photheraphy. J Investig Med. doi: 10.1177/jim-10815589221140594
5. Demirtas, M. S., Erdal H (2022) Evaluation of Thiol Disulfide Balance in Adolescents With Vitamin B12 Deficiency. DOI: https://doi.org/10.21203/rs.3.rs-1318028/v1
6. Erdal, H., Özcan, O., Turgut, F., Neşelioğlu, S., Erel Ö. (2022). Evaluation of dynamic thiol-disulfide balance and ischemia modified albumin levels in patients with chronic kidney disease. MKÜ Tıp Dergisi. 2022;13(47):237-242. https://doi.org/10.17944/mkutfd.947113
7. Erdal, H., Ciftciler, R., Tuncer, S. C., Ozcan, O. (2022). Evaluation of dynamic thiol-disulfide homeostasis and ischemia-modified albumin levels in patients with chronic lymphocytic leukemia. J Investig Med. doi: 10.1136/jim-2022-002568
8. Erdal, H., Demirtas, M. S., Tuncer, S.C., Ozcan O (2022).Thiol/disulfide homeostasis as a new oxidative stress marker in patients with neonatal transient tachypnea. Ann Clin Anal Med 2022, doi: 10.4328/ACAM.21457

9. Erel, O. (2005). A new automated colorimetric method for measuring total oxidant status. Clin Biochem, 38(12), 1103-1111. doi: 10.1016/j.clinbiochem.2005.08.008
10. Erel, O., & Neselioglu, S. (2014). A novel and automated assay for thiol/disulphide homeostasis. Clin Biochem, 47(18), 326-332. doi: 10.1016/j.clinbiochem.2014.09.026
11. Eryilmaz, M. A., Kozanhan, B., Solak, I., Cetinkaya, C. D., Neselioglu, S., & Erel, O. (2019). Thiol-disulfide homeostasis in breast cancer patients. J Cancer Res Ther, 15(5), 1062-1066. doi: 10.4103/jcrt.JCRT_553_17
12. Eryilmaz, O. G., Kansu-Celik, H., Erel, O., & Erdogan, S. (2017). Thiol/disulfide parameters as a novel oxidative marker in medical labor induction with oxytocin. Horm Mol Biol Clin Investig, 29(2), 61-65. doi: 10.1515/hmbci-2016-0032
13. Giri, T., Jiang, J., Xu, Z., McCarthy, R., Halabi, C. M., Tycksen, E., Palanisamy, A. (2022). Labor induction with oxytocin in pregnant rats is not associated with oxidative stress in the fetal brain. Sci Rep, 12(1), 3143. doi: 10.1038/s41598-022-07236-x
14. Karacor, T., Sak, S., Basaranoglu, S., Peker, N., Agacayak, E., Sak, M. E., Gul, T. (2017). Assessment of oxidative stress markers in cord blood of newborns to patients with oxytocin-induced labor. J Obstet Gynaecol Res, 43(5), 860-865. doi: 10.1111/jog.13263
15. Korkmaz, H., Onal, D., Alisik, M., Erel, O., & Pehlivanoglu, B. (2020). The impact of oxytocin on thiol/disulphide and malonyldialdehyde/glutathione homeostasis in stressed rats. Biol Chem, 401(11), 1283-1292. doi: 10.1515/hsz-2020-0190
16. Marconi, A. M. (2019). Recent advances in the induction of labor. F1000Res, 8. doi: 10.12688/f1000research.17587.1 Otal, Y., Kahraman, F. A., Haydar, F. G., & Erel, O. (2021). Dynamic thiol/disulfide homeostasis as oxidative stress marker in diabetic ketoacidosis. Turk J Med Sci, 51(2), 743-748. doi: 10.3906/sag-1904-55
17. Ozcan, O., Erdal, H., Ilhan, G., Demir, D., Gurpinar, A. B., Neselioglu, S., & Erel, O. (2018). Plasma Ischemia-Modified Albumin Levels and Dynamic Thiol/Disulfide Balance in Sickle Cell Disease: A Case-Control Study. Turk J Haematol, 35(4), 265-270. doi: 10.4274/tjh.2018.0119
18. Ozcan, O., Erdal, H., & Yonden, Z. (2015). İskemi-reperfüzyon hasari ve oksidatif stres ilişkisine biyokimyasal bakiş. Mustafa Kemal Üniversitesi Tıp Dergisi, 6(23), 27-33. Doi: 10.17944/mkutfd.54113
19. Özcan O, E. H., Çakırca G, Yönden Z (2015). Oxidative stress and its impacts on intracellular lipids, proteins and DNA. J Clin Exp Invest, 6(3), 331-336. doi: 10.5799/ahinjs.01.2015.03.0545
20. Rydahl, E., Eriksen, L., & Juhl, M. (2019). Effects of induction of labor prior to post-term in low-risk pregnancies: a systematic review. JBI Database System Rev Implement Rep, 17(2), 170-208. doi: 10.11124/JBISRIR-2017-003587
21. Saugstad, O. D. (2005). Oxidative stress in the newborn--a 30-year perspective. Biol Neonate, 88(3), 228-236. doi: 10.1159/000087586