CYTOTOXICITY OF RESIN MODIFIED GLASS IONOMER CEMENTS ON DENTAL PULP STEM CELLS

Şeyma Keskin; Fatih Şengül

doi:10.17567/ataunidfd.1030475

CYTOTOXICITY OF RESIN MODIFIED GLASS IONOMER CEMENTS ON DENTAL PULP STEM CELLS

Abstract

Aim: The aim of this study was to observe the cytotoxic effect of Resin modified Glass Ionomer Cement (RMGIC) on human dental pulp stem cells (DPSCs) proliferation by using xCELLigence®, a device that measures real-time cell viability and evaluates cytotoxic effects, and to determine the CC50 value on these cells for 72 hours. Materials and Methods: DPSCs obtained from the American Type Culture Collection were seeded on E-Plates. After 24 hours, three different dilutions (100%, 10% and 1%) of the elution obtained from RMGIC were added to three wells. DMEM solution was used as the control group. Real time cell index data were acquired by using xCELLigence® device for 72 hours. In order to compare cell index values, repeated-measures analysis of variance and linear regression analysis were used. Results: In contrast to the 100% dilution of RMGIC which exhibited toxic effect on DPSCs, its 1% dilution showed proliferative effect. And 10% dilution was similar to the control group. While the coefficient of determination was above 80% in all groups, it was found to be lower only in the RMGIC 100%-dilution group by 0.7%. Also, the regression coefficient was found to be significantly different from zero in all equations except RMGIC 100%-dilution group (p<0.001). CC50 values of RMGIC at the 24th, 48th and 72nd hours were 5.07%, 5.07% and 5.08%, respectively. Conclusion: In order to provide more reliable results, CC50 values determined in our study will guide the further studies to improve RMGICs by adding different molecules to its structure for reducing its cytotoxicity. Keywords: Glass ionomer cements, dental pulp stem cell, mesenchymal stem cell, cytotoxicity, xCELLigence Rezin ile Modifiye Edilmiş Cam İyonomer Simanların Diş Pulpası Kök Hücreleri Üzerindeki Sitotoksisitesi

Keywords

References

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Details

Primary Language

Turkish

Subjects

Dentistry

Journal Section

Research Article

Authors

Şeyma Keskin This is me
0000-0003-4861-678X
Türkiye

Fatih Şengül This is me
0000-0001-6087-148X
Türkiye

Publication Date

February 15, 2022

Submission Date

July 5, 2021

Acceptance Date

November 30, 2021

Published in Issue

Year 2022 Volume: 32 Number: 1

DOI

https://doi.org/10.17567/ataunidfd.1030475

IZ

https://izlik.org/JA34DP73AR

Cite

RIS / Bibtex

APA

Keskin, Ş., & Şengül, F. (2022). CYTOTOXICITY OF RESIN MODIFIED GLASS IONOMER CEMENTS ON DENTAL PULP STEM CELLS. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi, 32(1), 34-37. https://doi.org/10.17567/ataunidfd.1030475

AMA

1.Keskin Ş, Şengül F. CYTOTOXICITY OF RESIN MODIFIED GLASS IONOMER CEMENTS ON DENTAL PULP STEM CELLS. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi. 2022;32(1):34-37. doi:10.17567/ataunidfd.1030475

Chicago

Keskin, Şeyma, and Fatih Şengül. 2022. “CYTOTOXICITY OF RESIN MODIFIED GLASS IONOMER CEMENTS ON DENTAL PULP STEM CELLS”. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi 32 (1): 34-37. https://doi.org/10.17567/ataunidfd.1030475.

EndNote

Keskin Ş, Şengül F (February 1, 2022) CYTOTOXICITY OF RESIN MODIFIED GLASS IONOMER CEMENTS ON DENTAL PULP STEM CELLS. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi 32 1 34–37.

IEEE

[1]Ş. Keskin and F. Şengül, “CYTOTOXICITY OF RESIN MODIFIED GLASS IONOMER CEMENTS ON DENTAL PULP STEM CELLS”, Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi, vol. 32, no. 1, pp. 34–37, Feb. 2022, doi: 10.17567/ataunidfd.1030475.

ISNAD

Keskin, Şeyma - Şengül, Fatih. “CYTOTOXICITY OF RESIN MODIFIED GLASS IONOMER CEMENTS ON DENTAL PULP STEM CELLS”. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi 32/1 (February 1, 2022): 34-37. https://doi.org/10.17567/ataunidfd.1030475.

JAMA

1.Keskin Ş, Şengül F. CYTOTOXICITY OF RESIN MODIFIED GLASS IONOMER CEMENTS ON DENTAL PULP STEM CELLS. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi. 2022;32:34–37.

MLA

Keskin, Şeyma, and Fatih Şengül. “CYTOTOXICITY OF RESIN MODIFIED GLASS IONOMER CEMENTS ON DENTAL PULP STEM CELLS”. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi, vol. 32, no. 1, Feb. 2022, pp. 34-37, doi:10.17567/ataunidfd.1030475.

Vancouver

1.Şeyma Keskin, Fatih Şengül. CYTOTOXICITY OF RESIN MODIFIED GLASS IONOMER CEMENTS ON DENTAL PULP STEM CELLS. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi. 2022 Feb. 1;32(1):34-7. doi:10.17567/ataunidfd.1030475

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