CYTOTOXICITY OF DENTAL RESIN COMPOSITES: AN IN VITRO STUDY -

Abstract

Objective: The objective of this in vitro study was to evaluate the cytotoxic effects of two different resin based restorative meterials (Clearfil Majesty® ve Grandio Flow®) on human fibroblast cells via MTT [3(4,5Dimethylthiazol-2)-2,5 bromide] test. Material and Methods: Fibroblast cells derived from human amniotic fluid were used in this study. The samples prepared in 2 mm height and 8 mm diameter were placed into each of the wells in direct contact with the cell culture. None of the test materials was placed into the control group wells. The vitality rate of the cells was evaluated at the end of 24 hours, 72 hours and 7 days by means of MTT test results. Results: According to MTT findings, at the end of 24 hours, 72 hours and 7 days the decrease caused by the used materials in the number of the cells was ststistically significant. Both materials used found to be cytotoxic after 24 hours, 72 hours and 7 days. Conclusion: As the ingredients and filler rates of resin containing materials are important for cytotoxic effects, these specialities should be considered for material selection

Keywords

• Cell Culture,Cytotoxicity,MTT,Resin Monomers

DENTAL REZİN KOMPOZİTLERİN SİTOTOKSİSİTESİ: BİR İN VİTRO

Abstract

Amaç: Bu çalışmada iki farklı restoratif rezin materyalin (Clearfil Majesty^® ve Grandio Flow^®) insan fibroblast hücreleri üzerinde zamanla meydana getirdikleri sitotoksik etkilerin MTT [3-(4,5 DimetilTiazol-2)-2,5 DifenilTetrazolyum Bromit] testi ile değerlendirilmesi amaçlanmıştır.

Materyal ve Metot: Çalışmada insan amniyon hücresinden alınan fibroblastlar kullanılmıştır. Örnekler 8 mm çapında 2 mm yüksekliğinde hazırlanmıştır ve platelere aktarılan hücrelerle doğrudan temas edecek şekilde eklenmiştir. Kontrol grubuna ise herhangi bir materyal yerleştirilmemiştir. MTT testi ile 24 saat, 72 saat ve 7 gün sonunda gözlerdeki hücrelerin canlılığına bakılmıştır.

Bulgular: MTT testi sonuçlarına göre kullanılan materyallerin 24 saat, 72 saat ve 7 gün sonunda hücre canlılığını istatistiksel olarak anlamlı ölçüde azalttığı tespit edilmiştir. MTT değerlerine göre 24 saat, 72 saat ve 7 gün sonunda her iki materyal de toksik bulunmuştur.

Sonuç: Rezin esaslı materyallerin doldurucu içeriği ve oranı, sitotoksik etkileri açısından önemli olduğundan materyal seçiminde dikkate alınmalıdır.

Keywords

• Hücre Kültürü, MTT, Rezin Monomerler, Sitotoksisite

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