ROLE OF MOLECULAR MICROBIOLOGICAL TECHNIQUES IN ENDODONTIC INFECTIONS

Abstract

Endodontic infections are polymicrobial infections dominated by anaerobic species. The endodontic microflora has been traditionally investigated by microbiologic culture methods, which have been demonstrated to have several limitations when it comes to microbiological diagnosis. More recently, not only have findings from culture-based methods been confirmed, but they have also been significantly supplemented with those from culture-independent molecular genetic techniques. The results from molecular genetic studies impact remarkably on the knowledge of bacterial diversity in endodontic infections. Present review focuses on the role of molecular genetic techniques in endodontic infections

Keywords

• Endodontic Infections
• Molecular Mikrobiologic
• Techniques

ENDODONTİK ENFEKSİYONLARDA MİKROBİOLOJİK GENETİK TANI YÖNTEMLERİNİN ROLÜ

Abstract

ÖZET 

Endodontik enfeksiyonlar anaerobik türlerin baskın olarak bulunduğu polimikrobiyal enfeksiyonlardır. Geleneksel olarak endodontik mikroflora, mikrobiyolo- jik kültür yöntemleri kullanılarak araştırılmıştır. Ancak bu yöntemlerin mikrobiyolojik tanıda kısıtlı kalabildiği belirtilmektedir. Son yıllarda kültürden bağımsız moleküler genetik yöntemlerin kullanılması ile hem kültür temelli çalışmaların sonuçları desteklenmiş, hem de bu sonuçlara önemli derecede ilave bilgiler eklemiştir. Moleküler genetik çalışmaların sonuçları endodontik enfeksiyonlardaki bakteriyel çeşitlilik hakkındaki bilgilerin artmasını sağlamıştır. Bu derleme endodontik enfeksiyonlarda moleküler genetik yöntemlerin rolü hakkında bilgi sunmaktadır. 

Keywords

• Endodontik Enfeksiyonlar
• Moleküler Mikrobiyolojik
• Yöntemler

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