REAL–TIME CELL ANALYSIS OF CYTOTOXICITY OF DENTAL BONDING AGENTS ON HUMAN DENTAL PULP CELLS

Seyda Ersahan; Elif Aybala Oktay; Fidan Alakus Sabuncuoglu; Hüseyin Tort

doi:10.17567/ataunidfd.658109

REAL–TIME CELL ANALYSIS OF CYTOTOXICITY OF DENTAL BONDING AGENTS ON HUMAN DENTAL PULP CELLS

Abstract

Aim: To compare the cytotoxicity of dentin bonding agents on dental pulp cells through RTCA XCELLigence® impedance method, in order to investigate whether the self-etch adhesive would be more cytotoxic than two-step etch-and-rinse adhesive due to its higher acidity. Materials and Methods: Human dental pulp cells were obtained from healthy third molars extracted during routine clinical treatment. Two self-etching(Tokuyama Bond Force“Tb”;Shofu Beauti Bond“Bb”), and two total-etching(Dentsply Prime&Bond NT“Pb”;3M Adper Single Bond“Ab”) dentin-bonding agents(DBA) were diluted serially with culture medium at a ratio of 1:250, 1:500, 1:1000(v:v) and filtered. Cytotoxicity was identified by plating the pulp cells into the disposable E-plate 96 of xCELLigence® system. Results: Except for the 1:1000 dilution of Ab, each dilution of Ab was lower than the cell index of the control group at the 24th, and 48th hours. Furthermore, multiple comparison tests showed statistically significant differences between the control and each dilution of Ab (P = 0.000), except 1:1000 dilution of Ab at the 24th hour (P = 0.126). On the other hand, the cell indexes of 1:1000 dilution of Bb were higher than the cell index of the control at 24th and 48th hours, and there were statistically significant differences between them (P =0,000). At the 48th hour, 1:500 (P = 0.179) and 1:250 (P = 0.003) dilutions of Bb were lower than the control. Conclusion: Among the DBAs we used in our study, total-etch Ab showed the most cytotoxic effect. Conversely, self-etch Bb containing phosphoric acid monomer, showed the least cytotoxic effect. So, it may be more appropriate for clinical practices. Clinical relevance: Despite manufacturers’ claims, not all dentin bonding agents are biocompatible. Two-step etch-and-rinse adhesive may be less biocompatible than self-etch adhesive. Keywords: Human dental pulp cell, cell culture, cytotoxicity, dental bonding agent, xCELLigence® system.

Keywords

Human dental pulp cell,cell culture,cytotoxicity,dental bonding agent

References

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Details

Primary Language

English

Subjects

Dentistry

Journal Section

Research Article

Authors

Seyda Ersahan
Andorra

Elif Aybala Oktay This is me
Andorra

Fidan Alakus Sabuncuoglu This is me
Andorra

Hüseyin Tort This is me
Andorra

Publication Date

April 15, 2020

Submission Date

May 13, 2019

Acceptance Date

December 11, 2019

Published in Issue

Year 2020 Volume: 30 Number: 2

DOI

https://doi.org/10.17567/ataunidfd.658109

IZ

https://izlik.org/JA56GU97CL

Cite

RIS / Bibtex

APA

Ersahan, S., Oktay, E. A., Alakus Sabuncuoglu, F., & Tort, H. (2020). REAL–TIME CELL ANALYSIS OF CYTOTOXICITY OF DENTAL BONDING AGENTS ON HUMAN DENTAL PULP CELLS. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi, 30(2), 219-225. https://doi.org/10.17567/ataunidfd.658109

AMA

1.Ersahan S, Oktay EA, Alakus Sabuncuoglu F, Tort H. REAL–TIME CELL ANALYSIS OF CYTOTOXICITY OF DENTAL BONDING AGENTS ON HUMAN DENTAL PULP CELLS. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi. 2020;30(2):219-225. doi:10.17567/ataunidfd.658109

Chicago

Ersahan, Seyda, Elif Aybala Oktay, Fidan Alakus Sabuncuoglu, and Hüseyin Tort. 2020. “REAL–TIME CELL ANALYSIS OF CYTOTOXICITY OF DENTAL BONDING AGENTS ON HUMAN DENTAL PULP CELLS”. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi 30 (2): 219-25. https://doi.org/10.17567/ataunidfd.658109.

EndNote

Ersahan S, Oktay EA, Alakus Sabuncuoglu F, Tort H (April 1, 2020) REAL–TIME CELL ANALYSIS OF CYTOTOXICITY OF DENTAL BONDING AGENTS ON HUMAN DENTAL PULP CELLS. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi 30 2 219–225.

IEEE

[1]S. Ersahan, E. A. Oktay, F. Alakus Sabuncuoglu, and H. Tort, “REAL–TIME CELL ANALYSIS OF CYTOTOXICITY OF DENTAL BONDING AGENTS ON HUMAN DENTAL PULP CELLS”, Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi, vol. 30, no. 2, pp. 219–225, Apr. 2020, doi: 10.17567/ataunidfd.658109.

ISNAD

Ersahan, Seyda - Oktay, Elif Aybala - Alakus Sabuncuoglu, Fidan - Tort, Hüseyin. “REAL–TIME CELL ANALYSIS OF CYTOTOXICITY OF DENTAL BONDING AGENTS ON HUMAN DENTAL PULP CELLS”. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi 30/2 (April 1, 2020): 219-225. https://doi.org/10.17567/ataunidfd.658109.

JAMA

1.Ersahan S, Oktay EA, Alakus Sabuncuoglu F, Tort H. REAL–TIME CELL ANALYSIS OF CYTOTOXICITY OF DENTAL BONDING AGENTS ON HUMAN DENTAL PULP CELLS. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi. 2020;30:219–225.

MLA

Ersahan, Seyda, et al. “REAL–TIME CELL ANALYSIS OF CYTOTOXICITY OF DENTAL BONDING AGENTS ON HUMAN DENTAL PULP CELLS”. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi, vol. 30, no. 2, Apr. 2020, pp. 219-25, doi:10.17567/ataunidfd.658109.

Vancouver

1.Seyda Ersahan, Elif Aybala Oktay, Fidan Alakus Sabuncuoglu, Hüseyin Tort. REAL–TIME CELL ANALYSIS OF CYTOTOXICITY OF DENTAL BONDING AGENTS ON HUMAN DENTAL PULP CELLS. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi. 2020 Apr. 1;30(2):219-25. doi:10.17567/ataunidfd.658109