Atatürk Üniversitesi Diş Hekimliği Fakültesine başvuran bireylerin aerobik bakteriyel florasının mikrobiyal teşhis sistemi kullanılarak saptanması

Year 2012, Volume: 2012 Issue: 1, 26 - 30, 01.01.2012

Yrd. Doç. Dr. Nur Özdabak Yrd. Doç. Dr. Serpil Karaoğlanoğlu Yrd. Doç. Dr. Nilgün Akgül Prof. Dr. Nilgün Seven

Abstract

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Keywords

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Identification of aerobic bacterial flora in saliva of subjects who apply to the Faculty of Dentistry in Atatürk University by using microbial identification system

Abstract

Bu çalışmanın amacı, Atatürk Üniversitesi Diş Hekimliği Fakültesine başvuran hastaların tükürük aerobik bakteri florasını tanımlamaktır. Yöntem: DMFT indeksi 9-15 arasındaki 20 sağlıklı bireyin sitümüle edilmemiş tükürükleri toplandı.Toplam bakteri florası iki seçici olmayan medya, Nutrient Agar ve Sensitive Agar üzerindeki tükürük örneklerinden toplanmıştır. Izole bakteriyel organizmalar, fatty acid methyl ester (FAME) analizine dayanan Microbial Identification System (MIS; MIDI Inc., Newark, Del.) kullanılarak sınıflandırıldı . Sonuçlar. 75 bakteri suş izole edildi. Bunlardan, 67’sinin , 16 cins ve 34 türe ait olduğu saptanırken Kalan suşlar tanımlanamadı. En çok bulunan bakteri Streptococcus iken takip edenler Bacillus, Neisseria, Psychrobacter, Enterecoccus, Haemophilus, Staphylococcus, Paenibacillus, Camphylobacter, Micrococcus, Xanthobacter, Helikobakter, Actinomadura, Kocuria, Pseudomonas ve Cellulomonas oldu. Bilgilerimize dayanarak bu çalışma, bakteri türlerinden Xanthobacter Psychrobacter-phenylpyruvicus’u ağız florasında tanımlayan ilk literatürdür. Agilis, Actinomadura yumaensis ve Sonuç: Bu Çalışma;tükürük aerobik bakterilerinin tespiti için MIS’den yararlanılan ilk çalışmadır. Bizim sonuçlarımız MIS’in tükürük bakterinin tespiti için verimli ve hızlı ve doğru bir yöntem olduğunu desteklemektedir; ancak MIS veritabanlarının özellikle ağız bakteri florası olanlarının geliştirilmesi gerekmektedir

Keywords

yağ asidi metil ester, Mikrobiyal Tanımlama Sistemi, Ağız florası, tükürük

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