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IN VITRO EVALUATION OF THE CYTOTOXIC EFFECTS OF ROOT CANAL SEALERS WITH DIFFERENT CHEMICAL COMPOSITIONS

Year 2009, Volume: 2009 Issue: 2, 90 - 97, 01.02.2009

Dt. Ali Keleş Prof. Dr. Mustafa Köseoğlu Yrd. Doç.dr. K. Meltem Çolak Topçu Dt. Ö. Faruk Bayrak

Abstract

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Keywords

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References

  • Beltes P, Koulaouzidou E, Kotoula V, Kortsaris AH. In vitro evaluation of the cytotoxicity of calcium hydroxide-based root canal sealers. Endod Dent Traumatol 1995;11(5):245-9.
  • Orstavik D. Materials used for root canal obturation: technical, biological and clinical testing. Endod topics 2005;12:25-38.
  • McMichen FR, Pearson G, Rahbaran S, Gulabivala K. A comparative study of selected physical properties of five root-canal sealers. Int Endod J 2003;36(9):629-35.
  • Eldeniz AU, Mustafa K, Orstavik D, Dahl JE. Cytotoxicity of new resin-, calcium hydroxide- and silicone-based root canal sealers on fibroblasts derived from human gingiva and L929 cell lines. Int Endod J 2007;40(5):329-37.
  • Wataha JC. Biocompatibility of dental materials. In Anusavice KJ, ed. Phillips’ Science of Dental Materials. Philadelphia: Saunders; 1996:171-202.
  • Osorio RM, Hefti A, Vertucci FJ, Shawley AL. Cytotoxicity of endodontic materials. J Endod 1998;24(2):91-6.
  • Huang FM, Tai KW, Chou MY, Chang YC. Cytotoxicity of resin-, zinc oxide-eugenol-, and calcium hydroxide-based root canal sealers on human periodontal ligament cells and permanent V79 cells. Int Endod J 2002;35(2):153-8.
  • Schmalz G. Use of cell cultures for toxicity testing of dental materials--advantages and limitations. J Dent 1994;22 Suppl 2:S6-11.
  • International Organization for Standardization (1997) ISO-7405: Dentistry -- Preclinical evaluation of biocompatibility of medical devices used in dentistry -- Test methods for dental materials.
  • Can HE, Bala O, Kayaoğlu G, Alaçam T, Okur H. Yeni bir kök kanal dolgu patının sitotoksisite ve genotoksisitesinin in vitro olarak değerlendirilmesi. GÜ Dişhek Fak Derg 2002;19(2):23-27.
  • Miletic I, Devcic N, Anic I, Borcic J, Karlovic Z, Osmak M. The cytotoxicity of RoekoSeal and AH plus compared during different setting periods. J
  • Miletic I, Anic I, Karlovic Z, Marsan T, Pezelj- Ribaric S, Osmak M. Cytotoxic effect of four root filling 2000;16(6):287-90. Dent Traumatol
  • Citeau A, Guicheux J, Vinatier C, et al. In vitro biological effects of titanium rough surface obtained by calcium phosphate grid blasting. Biomaterials 2005;26(2):157-65.
  • Lonnroth EC, Dahl JE. Cytotoxicity of liquids and powders of chemically different dental materials evaluated using dimethylthiazol diphenyltetrazolium and neutral red tests. Acta Odontol Scand 2003;61(1):52-6.
  • Ogasawara T, Yoshimine Y, Yamamoto M, Akamine A. Biocompatibility of an experimental glass-ionomer cement sealer in rat mandibular bone. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2003;96(4):458-65.
  • Geurtsen W, Lehmann F, Spahl W, Leyhausen G. Cytotoxicity monomers/additives in permanent 3T3 and three resin composite in primary culture. J Endod
  • Takahara K, Onodera A, Matsumoto K. Toxicity of root canal sealers on rat bone cells in primary culture. Endod Dent Traumatol 1990;6(5):200-7.
  • Vajrabhaya L, Sithisarn P. Multilayer and monolayer cell cultures in a cytotoxicity assay of root canal sealers. Int Endod J 1997;30(2):141-4.
  • Ersev H, Schmalz G, Bayirli G, Schweikl H. Cytotoxic and mutagenic potencies of various root canal filling materials in eukaryotic and prokaryotic cells in vitro. J Endod 1999;25(5):359-63.
  • Huang FM, Hsieh YS, Tai KW, Chou MY, Chang YC. Induction of c-fos and c-jun protooncogenes expression by formaldehyde-releasing and epoxy resin-based root-canal sealers in human osteoblastic cells. J Biomed Mater Res 2002;59(3):460-5.
  • Schwarze T, Leyhausen G, Geurtsen W. Long-term cytocompatibility of various endodontic sealers using a new root canal model. J Endod 2002;28(11):749- 53.
  • Gerosa R, Menegazzi G, Borin M, Cavalleri G. Cytotoxicity evaluation of six root canal sealers. J Endod 1995;21(9):446-8.
  • Hauman CH, Love RM. Biocompatibility of dental materials used in contemporary endodontic therapy: a review. Part 2. Root-canal-filling materials. Int Endod J 2003;36(3):147-60.
  • Gomes BP, Pedroso JA, Jacinto RC, et al. In vitro evaluation of the antimicrobial activity of five root canal sealers. Braz Dent J 2004;15(1):30-5.
  • Södeberg TA. Effect of zinc oxide, rosin and resin acids and their combinations on bacterial growth and inflammatory cells. Scandinavian journal of plastic surgery.1990;24:1-87 surgery and hand
  • Lindqvist L, Otteskog P. Eugenol: liberation from dental materials and effect on human diploid fibroblast cells. Scand J Dent Res 1980;88(6):552-6.
  • Araki K, Suda H, Barbosa SV, Spangberg LS. Reduced cytotoxicity of a root canal sealer through eugenol substitution. J Endod 1993;19(11):554-7.
  • Maseki T, Nakata K, Kohsaka T, Kobayashi F, Hirano S, Nakamura H. Lack of correlation between the amount of eugenol released from zinc oxide- eugenol sealer and cytotoxicity of the sealer. J
  • Cohen BI, Pagnillo MK, Musikant BL, Deutsch AS. An in vitro study of the cytotoxicity of two root canal sealers. J Endod 2000;26(4):228-9.
  • Huang TH, Ding SJ, Hsu TZ, Lee ZD, Kao CT. Root canal sealers induce cytotoxicity and necrosis. J Mater Sci Mater Med 2004;15(7):767-71.
  • Tai KW, Huang FM, Huang MS, Chang YC. Assessment of the genotoxicity of resin and zinc- oxide eugenol-based root canal sealers using an in vitro mammalian test system. J Biomed Mater Res 2002;59(1):73-7.
  • Miletic I, Jukic S, Anic I, Zeljezic D, Garaj-Vrhovac V, Osmak M. Examination of cytotoxicity and mutagenicity of AH26 and AH Plus sealers. Int Endod J 2003;36(5):330-5.
  • Schweikl H, Schmalz G, Stimmelmayr H, Bey B. Mutagenicity of AH26 in an in vitro mammalian cell mutation assay. J Endod 1995;21(8):407-10.
  • Briseno BM, Willershausen B. Root canal sealer cytotoxicity on human gingival fibroblasts: 2. Silicone- and resin-based sealers. J Endod 1991;17(11):537-40.
  • Oztan MD, Yilmaz S, Kalayci A, Zaimoglu L. A comparison of the in vitro cytotoxicity of two root canal sealers. J Oral Rehabil 2003;30(4):426-9.
  • Bouillaguet S, Wataha JC, Lockwood PE, Galgano C, Golay A, Krejci I. Cytotoxicity and sealing properties of four classes of endodontic sealers evaluated by succinic dehydrogenase activity and confocal laser scanning microscopy. Eur J Oral Sci 2004;112(2):182-7.
  • Lestari F, Markovic B, Green AR, Chattopadhyay G, Hayes AJ. Comparative assessment of three in vitro exposure methods for combustion toxicity. J Appl Toxicol 2006;26(2):99-114.
  • Spangberg LS. In vitro assessment of the toxicity of endodontic materials. Int Endod J 1981;14(1):27- 33.

FARKLI İÇERİKLİ KÖK KANAL PATLARININ SİTOTOKSİSİTELERİNİN İN VİTRO OLARAK İNCELENMESİ

Year 2009, Volume: 2009 Issue: 2, 90 - 97, 01.02.2009

Dt. Ali Keleş Prof. Dr. Mustafa Köseoğlu Yrd. Doç.dr. K. Meltem Çolak Topçu Dt. Ö. Faruk Bayrak

Abstract

Bu çalışmamızın amacı, farklı sertleşme sürelerine sahip değişik içerikli kanal patlarının, 3T3 fare fibroblastları üzerinde oluşturdukları sitotoksisitenin in vitro olarak incelenmesi ve karşılaştırılmasıdır. AH Plus, AH 26, Endomethasone N, RSA ve Sealapex üreticilerinin talimatları doğrultusunda steril şartlarda karıştırılarak önceden hazırlanan 2 mm çapında ve 10 mm uzunluğundaki standart steril cam kalıplara yerleştirildi. Test örnekleri, taze, 3. gün ve 7. günde değerlendirilmek üzere 3 gruba ayrıldı. Farklı sertleşme zamanına sahip örnekler, 1 günlük, 4 günlük ve 7 günlük ekstraksiyon periyotları için 10 ml’lik kültür ortamda dilüe edildiler. Her dilüsyon periyodundan sonra ekstre alındı, 0.22 µm lik filtrede steril edildi ve 1:1, 1:2, 1:4, 1:8 oranlarında seyreltilerek, 1 günlük inkübasyon süresince 3T3 fare fibroblastlarına uygulandı. Yaşayabilen hücre oranları MTS (tetrazolium salt) analizi yapılarak değerlendirildi. Araştırmamızı oluşturan çeşitli değişkenler, oluşturdukları sitotoksisiteye göre, hem kendi aralarında hem de kendi içlerinde istatistiksel olarak değerlendirildi. Araştırmamızda, her kanal patının, her sertleşme ve ekstraksiyon süresinin ve her seyreltme oranının kendi aralarında istatistiksel olarak önemli farklılıklar gösterdiği bulundu (p AH 26 > Endomethasone N > AH Plus > RSA olarak sıralandı. Ayrıca çalışmada sertleşme zamanı ve seyreltilme oranı artıkça, sitotoksisite azalırken, ekstraksiyon süresi uzadıkça sitotoksisitenin arttığı görüldü

Keywords

Biyouyumluluk Kök kanal patları Sitotoksisite

References

  • Beltes P, Koulaouzidou E, Kotoula V, Kortsaris AH. In vitro evaluation of the cytotoxicity of calcium hydroxide-based root canal sealers. Endod Dent Traumatol 1995;11(5):245-9.
  • Orstavik D. Materials used for root canal obturation: technical, biological and clinical testing. Endod topics 2005;12:25-38.
  • McMichen FR, Pearson G, Rahbaran S, Gulabivala K. A comparative study of selected physical properties of five root-canal sealers. Int Endod J 2003;36(9):629-35.
  • Eldeniz AU, Mustafa K, Orstavik D, Dahl JE. Cytotoxicity of new resin-, calcium hydroxide- and silicone-based root canal sealers on fibroblasts derived from human gingiva and L929 cell lines. Int Endod J 2007;40(5):329-37.
  • Wataha JC. Biocompatibility of dental materials. In Anusavice KJ, ed. Phillips’ Science of Dental Materials. Philadelphia: Saunders; 1996:171-202.
  • Osorio RM, Hefti A, Vertucci FJ, Shawley AL. Cytotoxicity of endodontic materials. J Endod 1998;24(2):91-6.
  • Huang FM, Tai KW, Chou MY, Chang YC. Cytotoxicity of resin-, zinc oxide-eugenol-, and calcium hydroxide-based root canal sealers on human periodontal ligament cells and permanent V79 cells. Int Endod J 2002;35(2):153-8.
  • Schmalz G. Use of cell cultures for toxicity testing of dental materials--advantages and limitations. J Dent 1994;22 Suppl 2:S6-11.
  • International Organization for Standardization (1997) ISO-7405: Dentistry -- Preclinical evaluation of biocompatibility of medical devices used in dentistry -- Test methods for dental materials.
  • Can HE, Bala O, Kayaoğlu G, Alaçam T, Okur H. Yeni bir kök kanal dolgu patının sitotoksisite ve genotoksisitesinin in vitro olarak değerlendirilmesi. GÜ Dişhek Fak Derg 2002;19(2):23-27.
  • Miletic I, Devcic N, Anic I, Borcic J, Karlovic Z, Osmak M. The cytotoxicity of RoekoSeal and AH plus compared during different setting periods. J
  • Miletic I, Anic I, Karlovic Z, Marsan T, Pezelj- Ribaric S, Osmak M. Cytotoxic effect of four root filling 2000;16(6):287-90. Dent Traumatol
  • Citeau A, Guicheux J, Vinatier C, et al. In vitro biological effects of titanium rough surface obtained by calcium phosphate grid blasting. Biomaterials 2005;26(2):157-65.
  • Lonnroth EC, Dahl JE. Cytotoxicity of liquids and powders of chemically different dental materials evaluated using dimethylthiazol diphenyltetrazolium and neutral red tests. Acta Odontol Scand 2003;61(1):52-6.
  • Ogasawara T, Yoshimine Y, Yamamoto M, Akamine A. Biocompatibility of an experimental glass-ionomer cement sealer in rat mandibular bone. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2003;96(4):458-65.
  • Geurtsen W, Lehmann F, Spahl W, Leyhausen G. Cytotoxicity monomers/additives in permanent 3T3 and three resin composite in primary culture. J Endod
  • Takahara K, Onodera A, Matsumoto K. Toxicity of root canal sealers on rat bone cells in primary culture. Endod Dent Traumatol 1990;6(5):200-7.
  • Vajrabhaya L, Sithisarn P. Multilayer and monolayer cell cultures in a cytotoxicity assay of root canal sealers. Int Endod J 1997;30(2):141-4.
  • Ersev H, Schmalz G, Bayirli G, Schweikl H. Cytotoxic and mutagenic potencies of various root canal filling materials in eukaryotic and prokaryotic cells in vitro. J Endod 1999;25(5):359-63.
  • Huang FM, Hsieh YS, Tai KW, Chou MY, Chang YC. Induction of c-fos and c-jun protooncogenes expression by formaldehyde-releasing and epoxy resin-based root-canal sealers in human osteoblastic cells. J Biomed Mater Res 2002;59(3):460-5.
  • Schwarze T, Leyhausen G, Geurtsen W. Long-term cytocompatibility of various endodontic sealers using a new root canal model. J Endod 2002;28(11):749- 53.
  • Gerosa R, Menegazzi G, Borin M, Cavalleri G. Cytotoxicity evaluation of six root canal sealers. J Endod 1995;21(9):446-8.
  • Hauman CH, Love RM. Biocompatibility of dental materials used in contemporary endodontic therapy: a review. Part 2. Root-canal-filling materials. Int Endod J 2003;36(3):147-60.
  • Gomes BP, Pedroso JA, Jacinto RC, et al. In vitro evaluation of the antimicrobial activity of five root canal sealers. Braz Dent J 2004;15(1):30-5.
  • Södeberg TA. Effect of zinc oxide, rosin and resin acids and their combinations on bacterial growth and inflammatory cells. Scandinavian journal of plastic surgery.1990;24:1-87 surgery and hand
  • Lindqvist L, Otteskog P. Eugenol: liberation from dental materials and effect on human diploid fibroblast cells. Scand J Dent Res 1980;88(6):552-6.
  • Araki K, Suda H, Barbosa SV, Spangberg LS. Reduced cytotoxicity of a root canal sealer through eugenol substitution. J Endod 1993;19(11):554-7.
  • Maseki T, Nakata K, Kohsaka T, Kobayashi F, Hirano S, Nakamura H. Lack of correlation between the amount of eugenol released from zinc oxide- eugenol sealer and cytotoxicity of the sealer. J
  • Cohen BI, Pagnillo MK, Musikant BL, Deutsch AS. An in vitro study of the cytotoxicity of two root canal sealers. J Endod 2000;26(4):228-9.
  • Huang TH, Ding SJ, Hsu TZ, Lee ZD, Kao CT. Root canal sealers induce cytotoxicity and necrosis. J Mater Sci Mater Med 2004;15(7):767-71.
  • Tai KW, Huang FM, Huang MS, Chang YC. Assessment of the genotoxicity of resin and zinc- oxide eugenol-based root canal sealers using an in vitro mammalian test system. J Biomed Mater Res 2002;59(1):73-7.
  • Miletic I, Jukic S, Anic I, Zeljezic D, Garaj-Vrhovac V, Osmak M. Examination of cytotoxicity and mutagenicity of AH26 and AH Plus sealers. Int Endod J 2003;36(5):330-5.
  • Schweikl H, Schmalz G, Stimmelmayr H, Bey B. Mutagenicity of AH26 in an in vitro mammalian cell mutation assay. J Endod 1995;21(8):407-10.
  • Briseno BM, Willershausen B. Root canal sealer cytotoxicity on human gingival fibroblasts: 2. Silicone- and resin-based sealers. J Endod 1991;17(11):537-40.
  • Oztan MD, Yilmaz S, Kalayci A, Zaimoglu L. A comparison of the in vitro cytotoxicity of two root canal sealers. J Oral Rehabil 2003;30(4):426-9.
  • Bouillaguet S, Wataha JC, Lockwood PE, Galgano C, Golay A, Krejci I. Cytotoxicity and sealing properties of four classes of endodontic sealers evaluated by succinic dehydrogenase activity and confocal laser scanning microscopy. Eur J Oral Sci 2004;112(2):182-7.
  • Lestari F, Markovic B, Green AR, Chattopadhyay G, Hayes AJ. Comparative assessment of three in vitro exposure methods for combustion toxicity. J Appl Toxicol 2006;26(2):99-114.
  • Spangberg LS. In vitro assessment of the toxicity of endodontic materials. Int Endod J 1981;14(1):27- 33.
There are 38 citations in total.

Details

Primary Language Turkish
Journal Section Articles
Authors

Dt. Ali Keleş This is me

Prof. Dr. Mustafa Köseoğlu This is me

Yrd. Doç.dr. K. Meltem Çolak Topçu This is me

Dt. Ö. Faruk Bayrak This is me

Publication Date February 1, 2009
Published in Issue Year 2009 Volume: 2009 Issue: 2

Cite

APA Keleş, D. A., Köseoğlu, P. D. M., Topçu, Y. D. K. M. Ç., Bayrak, D. Ö. F. (2009). FARKLI İÇERİKLİ KÖK KANAL PATLARININ SİTOTOKSİSİTELERİNİN İN VİTRO OLARAK İNCELENMESİ. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi, 2009(2), 90-97.
AMA Keleş DA, Köseoğlu PDM, Topçu YDKMÇ, Bayrak DÖF. FARKLI İÇERİKLİ KÖK KANAL PATLARININ SİTOTOKSİSİTELERİNİN İN VİTRO OLARAK İNCELENMESİ. Ata Diş Hek Fak Derg. February 2009;2009(2):90-97.
Chicago Keleş, Dt. Ali, Prof. Dr. Mustafa Köseoğlu, Yrd. Doç.dr. K. Meltem Çolak Topçu, and Dt. Ö. Faruk Bayrak. “FARKLI İÇERİKLİ KÖK KANAL PATLARININ SİTOTOKSİSİTELERİNİN İN VİTRO OLARAK İNCELENMESİ”. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi 2009, no. 2 (February 2009): 90-97.
EndNote Keleş DA, Köseoğlu PDM, Topçu YDKMÇ, Bayrak DÖF (February 1, 2009) FARKLI İÇERİKLİ KÖK KANAL PATLARININ SİTOTOKSİSİTELERİNİN İN VİTRO OLARAK İNCELENMESİ. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi 2009 2 90–97.
IEEE D. A. Keleş, P. D. M. Köseoğlu, Y. D. K. M. Ç. Topçu, and D. Ö. F. Bayrak, “FARKLI İÇERİKLİ KÖK KANAL PATLARININ SİTOTOKSİSİTELERİNİN İN VİTRO OLARAK İNCELENMESİ”, Ata Diş Hek Fak Derg, vol. 2009, no. 2, pp. 90–97, 2009.
ISNAD Keleş, Dt. Ali et al. “FARKLI İÇERİKLİ KÖK KANAL PATLARININ SİTOTOKSİSİTELERİNİN İN VİTRO OLARAK İNCELENMESİ”. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi 2009/2 (February 2009), 90-97.
JAMA Keleş DA, Köseoğlu PDM, Topçu YDKMÇ, Bayrak DÖF. FARKLI İÇERİKLİ KÖK KANAL PATLARININ SİTOTOKSİSİTELERİNİN İN VİTRO OLARAK İNCELENMESİ. Ata Diş Hek Fak Derg. 2009;2009:90–97.
MLA Keleş, Dt. Ali et al. “FARKLI İÇERİKLİ KÖK KANAL PATLARININ SİTOTOKSİSİTELERİNİN İN VİTRO OLARAK İNCELENMESİ”. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi, vol. 2009, no. 2, 2009, pp. 90-97.
Vancouver Keleş DA, Köseoğlu PDM, Topçu YDKMÇ, Bayrak DÖF. FARKLI İÇERİKLİ KÖK KANAL PATLARININ SİTOTOKSİSİTELERİNİN İN VİTRO OLARAK İNCELENMESİ. Ata Diş Hek Fak Derg. 2009;2009(2):90-7.

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