POLYMERASE CHAIN REACTION (PCR) AND ENDODONTIC MICROBIOLOGY

Year 2006, Volume: 2006 Issue: 2, 61 - 65, 01.02.2006

Dt. Özgür İlke Atasoy Ulusoy Prof. Dr. Güliz Görgül

Abstract

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Keywords

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POLİMERAZ ZİNCİR REAKSİYONU (PCR) VE ENDODONTİK MİKROBİYOLOJİ

Abstract

Bu makale polimeraz zincir reaksiyonunun (Polymerase Chain Reaction-PCR) endodontide kullan m n , endodontik mikroorganizmalar n varl 6 n n ve prevalanslar n n belirlenmesinde sa6lad 6 avantajlargözden geçirmektedir. Kültür yöntemiyle üretilemeyen mikroorganizmalar n 16S rRNA bölgelerinin PCR kullan larak ço6alt lmas moleküler mikrobiyolojide ç 6 raçm t r. PCR kültür yöntemlerine göre daha hassas, özgül, h zl d r ve daha kesin sonuçlar sa6lar. Bu metodlar n yerinde kullan m , endodontik hastal klar nnedenlerinin belirlenmesine, anla lmas na ve tedavi stratejilerinin geli tirilmesine önemli katk lar sa6layacakt r

Keywords

PCR, virulans, mikroorganizma, kök kanal

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