THE EVALUATION OF THE COLONIZATION AND BIOFILM FORMATION CAPACITY OF THE CANDIDA SPECIES ISOLATED FROM DENTURE WEARER PATIENTS

Year 2019, Volume: 29 Issue: 3, 387 - 393, 16.07.2019

Suleyman Emre Meşeli Gonca Erköse Genç Gizem Çolakoğlu Suleyman Pelit Esra Koç Birsay Gümrü Tarçın

https://doi.org/10.17567/ataunidfd.566861

Abstract

Abstract

 Aim: CAim: Candida species, although being a member of the oral flora, may exhibit pathological features under certain conditions. The aim of this study was to determine the distribution and biofilm production of Candida species that were isolated from denture related stomatitis (DRS) patients and to compare with healthy subjects.
andida species, although being a member of the oral flora, may exhibit pathological features under certain conditions. The aim of this study was to determine the distribution and biofilm production of Candida species that were isolated from denture related stomatitis (DRS) patients and to compare with healthy subjects.

Materials and Methods: The 56 non-smoker, systemically healthy, maxillary removable
denture wearer subjects included in this study and diagnosed as DRS(N=27) and
healthy(N=29). Samples from
the palatal mucosal surface of patients were collected by sterile swabs during
20 seconds. Cultivation and selective isolation of Candida species were
performed on CHROMagar (CHROMagar^® Candida, CHROMagar, Paris,
France) at 37ºC for 2 days. Isolated Candida species were identified with API
ID 32 C (bioMérieux^®, France). Biofilm formation by Candida species
were determined by a visual tube method.

Results: The prevalence of the yeasts in the healthy group was found to be
37.94%, while in the DRS group it was 100% (p<0.001). The isolation rate of C. albicans in patients with DRS
significantly higher (p<0.001). Biofilm formation was observed in a total of
37 oral yeast isolates, 9 isolates from healthy denture wearers and 28 isolates
from DRS patients. The number of C.
albicans and C. glabrata isolates
showing biofilm formation ability in the DRS group was significantly higher
than the healthy group (p<0.05).

Conclusions: Within
the limitations of this study, our results suggest that Candida species play a
major role on the development of DRS. While C.
albicans was the most isolated species in DRS, C. glabrata was as important as C.
albicans on the pathogenesis of DRS.

 keywords: Biofilms, Candida, denture
stomatitis, mycology, oral pathology

HAREKETLİ PROTEZ KULLANAN HASTALARDA KANDİDA TÜRLERİNİN
KOLONİZASYONUNUN VE BİYOFİLM OLUŞTURMA KAPASİTELERİNİN DEĞERLENDİRİLMESİ

Öz

Amaç: Candida türleri, oral floranın doğal üyesi olmakla birlikte, belirli
koşullar altında patolojik özellikler sergileyebilir. Bu çalışmanın amacı, protez
stomatiti (PS) olan hastalardan ve protez kullanan sağlıklı bireylerden elde
edilen Candida türlerinin dağılımını ve biyofilm üretime kapasitelerini
karşılaştırmaktır

Gereç ve Yöntem: Sigara
içmeyen, sistemik olarak sağlıklı, maksiller tam damak protez kullanan 56 kişi
bu çalışmaya dahil edildi. Katılımcılar PS 
(N = 27) ve sağlıklı (N = 29) olmak üzere gruplandırıldı. Hastaların
palatal mukoza yüzeyinde 20 saniye boyunca steril swablar gezdirilerek
sürüntü örnekleri elde edildi. Candida türlerinin kültivasyon ve seçici
izolasyonu 2 gün boyunca 37 ° C'de CHROMagar (CHROMagar® Candida, CHROMagar,
Paris, Fransa) besiyerinde gerçekleştirildi. İzole edilen Candida türlerinin
identifikasyonu API ID 32 C (bioMérieux®, Fransa) ile gerçekleştirildi. Candida
türlerinin biyofilm oluşturma potansiyelleri görsel tüp yöntemi ile saptandı
Bulgular: Sağlıklı gruptaki
mayaların prevalansı % 37.94 iken, PS grubunda % 100'dü (p <0.001). PS'li hastalarda C.
albicans'ın izolasyon oranı anlamlı derecede daha yüksekti (p <0.001). Sağlıklı katılımcılardaki
9 PS katılımcılardaki 28 izolatta biyofilm oluşumu gözlendi. PS grubunda
biyofilm oluşumunu gösteren C. albicans
ve C. glabrata izolatlarının sayısı
sağlıklı gruba göre anlamlı olarak yüksekti (p <0.05).

Sonuç: Bu çalışmanın sınırları dahilinde, sonuçlarımız Candida
türlerinin PS'nin gelişiminde önemli bir rol oynadığını göstermektedir. C. albicans, PS'de en sık izole edilen
tür olsa da C. glabrata da PS'nin
patogenezinde C. albicans kadar
önemlidir.

Anahtar Kelimeler: Biyofilm, Kandida, protez stomatitisi,
mikoloji, oral patoloji

Keywords

Biofilms, Candida, denture stomatitis, mycology

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