Year 2019, Volume 29 , Issue 3, Pages 387 - 393 2019-07-16

THE EVALUATION OF THE COLONIZATION AND BIOFILM FORMATION CAPACITY OF THE CANDIDA SPECIES ISOLATED FROM DENTURE WEARER PATIENTS

Suleyman Emre MEŞELİ [1] , Gonca ERKÖSE GENÇ [2] , Gizem ÇOLAKOĞLU [3] , Suleyman PELİT [4] , Esra KOÇ [5] , Birsay GÜMRÜ TARÇIN [6]


Abstract

 Aim: CAim: Candida species, although being a member of the oral flora, may exhibit pathological features under certain conditions. The aim of this study was to determine the distribution and biofilm production of Candida species that were isolated from denture related stomatitis (DRS) patients and to compare with healthy subjects.
andida species, although being a member of the oral flora, may exhibit pathological features under certain conditions. The aim of this study was to determine the distribution and biofilm production of Candida species that were isolated from denture related stomatitis (DRS) patients and to compare with healthy subjects.

Materials and Methods: The 56 non-smoker, systemically healthy, maxillary removable denture wearer subjects included in this study and diagnosed as DRS(N=27) and healthy(N=29). Samples from the palatal mucosal surface of patients were collected by sterile swabs during 20 seconds. Cultivation and selective isolation of Candida species were performed on CHROMagar (CHROMagar® Candida, CHROMagar, Paris, France) at 37ºC for 2 days. Isolated Candida species were identified with API ID 32 C (bioMérieux®, France). Biofilm formation by Candida species were determined by a visual tube method.

Results: The prevalence of the yeasts in the healthy group was found to be 37.94%, while in the DRS group it was 100% (p<0.001). The isolation rate of C. albicans in patients with DRS significantly higher (p<0.001). Biofilm formation was observed in a total of 37 oral yeast isolates, 9 isolates from healthy denture wearers and 28 isolates from DRS patients. The number of C. albicans and C. glabrata isolates showing biofilm formation ability in the DRS group was significantly higher than the healthy group (p<0.05).

Conclusions: Within the limitations of this study, our results suggest that Candida species play a major role on the development of DRS. While C. albicans was the most isolated species in DRS, C. glabrata was as important as C. albicans on the pathogenesis of DRS.

 keywords: Biofilms, Candida, denture stomatitis, mycology, oral pathology

HAREKETLİ PROTEZ KULLANAN HASTALARDA KANDİDA TÜRLERİNİN KOLONİZASYONUNUN VE BİYOFİLM OLUŞTURMA KAPASİTELERİNİN DEĞERLENDİRİLMESİ

Öz

Amaç: Candida türleri, oral floranın doğal üyesi olmakla birlikte, belirli koşullar altında patolojik özellikler sergileyebilir. Bu çalışmanın amacı, protez stomatiti (PS) olan hastalardan ve protez kullanan sağlıklı bireylerden elde edilen Candida türlerinin dağılımını ve biyofilm üretime kapasitelerini karşılaştırmaktır

Gereç ve Yöntem: Sigara içmeyen, sistemik olarak sağlıklı, maksiller tam damak protez kullanan 56 kişi bu çalışmaya dahil edildi. Katılımcılar PS  (N = 27) ve sağlıklı (N = 29) olmak üzere gruplandırıldı. Hastaların palatal mukoza yüzeyinde 20 saniye boyunca steril swablar gezdirilerek sürüntü örnekleri elde edildi. Candida türlerinin kültivasyon ve seçici izolasyonu 2 gün boyunca 37 ° C'de CHROMagar (CHROMagar® Candida, CHROMagar, Paris, Fransa) besiyerinde gerçekleştirildi. İzole edilen Candida türlerinin identifikasyonu API ID 32 C (bioMérieux®, Fransa) ile gerçekleştirildi. Candida türlerinin biyofilm oluşturma potansiyelleri görsel tüp yöntemi ile saptandı
Bulgular: Sağlıklı gruptaki mayaların prevalansı % 37.94 iken, PS grubunda % 100'dü (p <0.001). PS'li hastalarda C. albicans'ın izolasyon oranı anlamlı derecede daha yüksekti (p <0.001). Sağlıklı katılımcılardaki 9 PS katılımcılardaki 28 izolatta biyofilm oluşumu gözlendi. PS grubunda biyofilm oluşumunu gösteren C. albicans ve C. glabrata izolatlarının sayısı sağlıklı gruba göre anlamlı olarak yüksekti (p <0.05).

Sonuç: Bu çalışmanın sınırları dahilinde, sonuçlarımız Candida türlerinin PS'nin gelişiminde önemli bir rol oynadığını göstermektedir. C. albicans, PS'de en sık izole edilen tür olsa da C. glabrata da PS'nin patogenezinde C. albicans kadar önemlidir.

Anahtar Kelimeler: Biyofilm, Kandida, protez stomatitisi, mikoloji, oral patoloji







Biofilms, Candida, denture stomatitis, mycology
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Primary Language en
Subjects Dental
Journal Section Araştırma Makalesi
Authors

Orcid: 0000-0002-8922-155X
Author: Suleyman Emre MEŞELİ

Orcid: 0000-0002-8983-9675
Author: Gonca ERKÖSE GENÇ

Orcid: 0000-0002-9471-9993
Author: Gizem ÇOLAKOĞLU

Orcid: 0000-0002-0028-4264
Author: Suleyman PELİT

Orcid: 0000-0003-0435-3607
Author: Esra KOÇ

Orcid: 0000-0002-7734-4755
Author: Birsay GÜMRÜ TARÇIN

Dates

Publication Date : July 16, 2019

Bibtex @research article { ataunidfd566861, journal = {Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi}, issn = {1300-9044}, eissn = {2667-5161}, address = {}, publisher = {Ataturk University}, year = {2019}, volume = {29}, pages = {387 - 393}, doi = {10.17567/ataunidfd.566861}, title = {THE EVALUATION OF THE COLONIZATION AND BIOFILM FORMATION CAPACITY OF THE CANDIDA SPECIES ISOLATED FROM DENTURE WEARER PATIENTS}, key = {cite}, author = {MEŞELİ, Suleyman Emre and ERKÖSE GENÇ, Gonca and ÇOLAKOĞLU, Gizem and PELİT, Suleyman and KOÇ, Esra and GÜMRÜ TARÇIN, Birsay} }
APA MEŞELİ, S , ERKÖSE GENÇ, G , ÇOLAKOĞLU, G , PELİT, S , KOÇ, E , GÜMRÜ TARÇIN, B . (2019). THE EVALUATION OF THE COLONIZATION AND BIOFILM FORMATION CAPACITY OF THE CANDIDA SPECIES ISOLATED FROM DENTURE WEARER PATIENTS. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi , 29 (3) , 387-393 . DOI: 10.17567/ataunidfd.566861
MLA MEŞELİ, S , ERKÖSE GENÇ, G , ÇOLAKOĞLU, G , PELİT, S , KOÇ, E , GÜMRÜ TARÇIN, B . "THE EVALUATION OF THE COLONIZATION AND BIOFILM FORMATION CAPACITY OF THE CANDIDA SPECIES ISOLATED FROM DENTURE WEARER PATIENTS". Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi 29 (2019 ): 387-393 <https://dergipark.org.tr/en/pub/ataunidfd/issue/47796/566861>
Chicago MEŞELİ, S , ERKÖSE GENÇ, G , ÇOLAKOĞLU, G , PELİT, S , KOÇ, E , GÜMRÜ TARÇIN, B . "THE EVALUATION OF THE COLONIZATION AND BIOFILM FORMATION CAPACITY OF THE CANDIDA SPECIES ISOLATED FROM DENTURE WEARER PATIENTS". Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi 29 (2019 ): 387-393
RIS TY - JOUR T1 - THE EVALUATION OF THE COLONIZATION AND BIOFILM FORMATION CAPACITY OF THE CANDIDA SPECIES ISOLATED FROM DENTURE WEARER PATIENTS AU - Suleyman Emre MEŞELİ , Gonca ERKÖSE GENÇ , Gizem ÇOLAKOĞLU , Suleyman PELİT , Esra KOÇ , Birsay GÜMRÜ TARÇIN Y1 - 2019 PY - 2019 N1 - doi: 10.17567/ataunidfd.566861 DO - 10.17567/ataunidfd.566861 T2 - Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi JF - Journal JO - JOR SP - 387 EP - 393 VL - 29 IS - 3 SN - 1300-9044-2667-5161 M3 - doi: 10.17567/ataunidfd.566861 UR - https://doi.org/10.17567/ataunidfd.566861 Y2 - 2019 ER -
EndNote %0 Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi THE EVALUATION OF THE COLONIZATION AND BIOFILM FORMATION CAPACITY OF THE CANDIDA SPECIES ISOLATED FROM DENTURE WEARER PATIENTS %A Suleyman Emre MEŞELİ , Gonca ERKÖSE GENÇ , Gizem ÇOLAKOĞLU , Suleyman PELİT , Esra KOÇ , Birsay GÜMRÜ TARÇIN %T THE EVALUATION OF THE COLONIZATION AND BIOFILM FORMATION CAPACITY OF THE CANDIDA SPECIES ISOLATED FROM DENTURE WEARER PATIENTS %D 2019 %J Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi %P 1300-9044-2667-5161 %V 29 %N 3 %R doi: 10.17567/ataunidfd.566861 %U 10.17567/ataunidfd.566861
ISNAD MEŞELİ, Suleyman Emre , ERKÖSE GENÇ, Gonca , ÇOLAKOĞLU, Gizem , PELİT, Suleyman , KOÇ, Esra , GÜMRÜ TARÇIN, Birsay . "THE EVALUATION OF THE COLONIZATION AND BIOFILM FORMATION CAPACITY OF THE CANDIDA SPECIES ISOLATED FROM DENTURE WEARER PATIENTS". Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi 29 / 3 (July 2019): 387-393 . https://doi.org/10.17567/ataunidfd.566861
AMA MEŞELİ S , ERKÖSE GENÇ G , ÇOLAKOĞLU G , PELİT S , KOÇ E , GÜMRÜ TARÇIN B . THE EVALUATION OF THE COLONIZATION AND BIOFILM FORMATION CAPACITY OF THE CANDIDA SPECIES ISOLATED FROM DENTURE WEARER PATIENTS. Ata Diş Hek Fak Derg. 2019; 29(3): 387-393.
Vancouver MEŞELİ S , ERKÖSE GENÇ G , ÇOLAKOĞLU G , PELİT S , KOÇ E , GÜMRÜ TARÇIN B . THE EVALUATION OF THE COLONIZATION AND BIOFILM FORMATION CAPACITY OF THE CANDIDA SPECIES ISOLATED FROM DENTURE WEARER PATIENTS. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi. 2019; 29(3): 393-387.