SODIUM THIOSULPHATE AND TWEEN 80 PREVENT THE FORMATION OF PARA-CHLOROANILINE IN THE IRRIGATING SOLUTIONS

Year 2020, Volume: 30 Issue: 1, 61 - 65, 15.01.2020

Ezgi Doğanay Yıldız , Hakan Arslan Gizem Taş Eyüp Candaş Gündoğdu Ali Keskın Alper Yıldırım

https://doi.org/10.17567/ataunidfd.637010

Abstract

Aim: The aim of the present study was to evaluate
the effect of using sodium thiosulphate and Tween 80 to prevent the
formation of para-chloroaniline (PCA) between NaOCl and CHX in the apically
extruded irrigating solutions.

Materials and Methods: Twenty-five extracted human
mandibular premolar teeth were instrumented up to size 40. To collect the
apically extruded irrigating solution, the roots were placed into the glass
vial, and the specimens were divided into 5 groups according to the irrigation
protocols, as follows; positive control, negative control, distilled water as
intermediate flush between NaOCl and CHX, sodium thiosulphate irrigation
between NaOCl and CHX, and Tween 80 irrigation between CHX and NaOCl. The
collected liquid was centrifuged and the precipitate was analyzed using 1H NMR
spectra. The data were analyzed with chi-square test at 95% confidence
level.

Results: PCA was detected for all specimens in the
positive control group. PCA was absent in the negative control group.
Although distilled water irrigation did not prevent the formation of PCA,
sodium thiosulphate and Tween 80 prevented the formation of PCA between
NaOCl-CHX and CHX-NaOCl, respectively.

Conclusion: Within the limitations of the present study, sodium
thiosulphate or Tween 80 prevented the formation of PCA between NaOCl-CHX
and CHX-NaOCl, respectively.

Keywords: chlorhexidine, para chloroaniline, sodium
thiosulphate

Sodyum Tiyosülftat ve Tween 80 İrrigasyon Solüsyonları İçinde
Para-kloroanilin Formasyonunu Engeller

Öz

Amaç:
Bu çalışmanın amacı NaOCl ve CHX arasında sodyum tiyosülfat ve Tween 80
kullanımının apikalden ekstrüze olan irrigasyon solüsyonları içinde PCA
formasyonunun önlenmesine etkisinin değerlendirilmesidir.

Gereç ve Yöntem:
Yirmi-beş çekilmiş mandibular premolar diş 40 numaraya kadar genişletildi.
Apikalden ekstrüze olan irrigasyon solüsyonlarının toplanması için, kökler cam
şişe içine yerleştirildi, örnekler irrigasyon protokolüne göre 5 gruba ayrıldı:
pozitif kontrol, negatif kontrol, NaOCl ve CHX arasında distile su ile yıkama,
NaOCl ve CHX arasında sodyum tiyosülfat ile yıkama, CHX ve NaOCl arasında Tween
80 ile yıkama. Toplanan sıvı santrifüje edildi ve çökelti 1H NMR spectra ile
analiz edildi. Veriler ki-kare testi ile %95 güven aralığında analiz edildi.

Bulgular:
Pozitif kontrol grubundaki tüm örneklerde PCA tespit edildi ve negatif
gruptaki örneklerin hiçbirinde tespit edilmedi. Distile su irrigayonu PCA
oluşumunu önlememesine rağmen, sodium tiyosülfat ve Tween 80 sırasıyla NaOCl
-CHX ve CHX- NaOCl arasında kullanımla PCA formasyonunu engelledi.

Sonuç: Bu çalışmanın
limitasyonları dahilinde, sodium tiyosülfat ve Tween 80, sırasıyla NaOCl -CHX
ve CHX- NaOCl arasında kullanımla PCA formasyonunu engellemektedir.

Anahtar
kelimeler: klorheksidin, para kloroanilin, sodyum tiyosülfat

Keywords

chlorhexidine, para chloroaniline

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