EVALUATİON OF OXİDATİVE STATUS İN PATİENTS WİTH CHRONİC PERİODONTİTİS AND ADDİTİONAL TOBACCO ABUSE: A CROSS-SECTİONAL STUDY

Year 2020, Volume: 30 Issue: 4, 536 - 544, 15.10.2020

Didem Özkal Eminoğlu Varol Çanakçı

https://doi.org/10.17567/ataunidfd.778795

Abstract

Aim: The aim of the present study was to investigate the 8-hydroxy-2´deoxyguanosine (8-OHdG) and melatonin (MLT) levels in saliva as well as myeloperoxidase (MPO) levels in gingival clevicular fluid (GCF) in smoker and nonsmoker chronic periodontitis (CP) patients and periodontally healthy individuals.
Methods: 4 groups of 15 applicants were formed in accordance with the research protocole as follows; periodontally healthy nonsmokers (CP-S-), periodontally healthy smokers (CP-S+), nonsmoker periodontitis patients (CP+S-) and smoker periodontitis patients (CP+S+). Clinical periodontal parameters were recorded before taking the GCF and saliva samples. 8-OHdG, MLT and MPO levels were measured biochemically.
Results: The lowest 8-OHdG and MPO levels were recorded in the (CP-S-) group (p<0.05). 8-OHdG and MPO levels were statistically higher in (CP-S+) group than (CP-S-) group (p<0.05). MLT levels in saliva were statistically higher in periodontally healthy (CP-) groups than CP groups (p<0.05).
Conclusions: CP causes a significant difference on the parameters connected with oxidative stress. For periodontally healthy applicants, smoking leads to statistically significant changes on the parameters in general; while the effect of smoking to the parameters was found insignificant in the CP groups. Findings of this study reveal the role of smoking on the development of periodontitis, due to negatively affecting the parameters linked to oxidative stress, that are crucial in the patogenesis of periodontal diseases.
KEY WORDS: 8-hydroxy-2´-deoxyguanosine (8-OHdG), chronic periodontitis (CP), melatonin (MLT), myeloperoxidase (MPO), oxidative stres (OS), smoking, tobacco.

Kronik Periodontitis Ve Sigara Kullananımının Oksidatif Durum Üzerine Olan Etkisinin Değerlendirilmesi
Öz
Amaç: Bu çalışmanın amacı; kronik periodontitisli ve periodontal açıdan sağlıklı bireylerin DOS ve tükürük numunelerinde sigaranın MLT, MPO ve 8-OHdG düzeyleri üzerine muhtemel etkilerinin değerlendirilmesidir.
Gereç ve yöntem : Çalışma protokolü gereği her biri 15 katılımcıdan oluşan 4 grup oluşturuldu; periodontal olarak sağlıklı ve sigara içemeyen grup (P-S-), periodontal olarak sağlıklı ve sigara içen grup (P-S+), kronik periodontitisli ve sigara içmeyen grup (P+S-) ile kronik periodontitisli ve sigara içen grup (P+S+). DOS ve tükürük örnekleri alınmadan önce klinik periodontal parametreler kaydedildi. MLT, MPO ve 8-OHdG düzeyleri biyokimyasal ve olarak ölçüldü.
Bulgular : En yüksek MPO ve 8-OHdGdüzeyleri ile en düşük MLT düzeyleri (P+S+) grubunda tespit edildi. En yüksek MLT düzeyi ise (P-S-) grubunda gözlendi. Sigara kullanımı ve kronik periodontitisin biyokimyasal ve histopatolojik parametreleri benzer şekilde etkilediği tespit edildi.
Sonuç : KP’nin oksidatif stres ile ilişkili parametrelerde anlamlı düzeyde farklılık oluşturduğu gözlendi. Sigara kullanımının genel olarak periodontal sağlıklı bireylerde parametrelerde belirgin bir değişime yol açtığı, periodontitisli gruplarda ise bu değişimin istatistiksel olarak anlamlı olmadığı görüldü. Bulgularımız, sigara içmenin periodontal hastalıkların patogenezinde rol alan oksitatif parametreleri olumsuz yönde etkileyerek periodontitis gelişiminde rol oynayabileceğini göstermektedir.
Anahtar Kelimeler: Isı şok proteinleri 60-70, kronik periodontitis, melatonin, myeloperoksidaz, oksitatif stress, sigara, 8-hydroxy-2'-deoxyguanosine.

Keywords

8-hydroxy-2´-deoxyguanosine (8-OHdG), chronic periodontitis (CP), melatonin (MLT)

EVALUATION OF THE EFFECT OF CHRONIC PERIODONTITIS AND ADDITIONAL TOBACCO ABUSE ON OXIDATIVE STATUS : A CROSS-SECTIONAL STUDY

Abstract

Aim: The aim of the present study was to investigate the 8-hydroxy-2´deoxyguanosine (8-OHdG) and melatonin (MLT) levels in saliva as well as myeloperoxidase (MPO) levels in gingival clevicular fluid (GCF) in smoker and nonsmoker chronic periodontitis (CP) patients and periodontally healthy individuals.
Methods: 4 groups of 15 applicants were formed in accordance with the research protocole as follows; periodontally healthy nonsmokers (CP-S-), periodontally healthy smokers (CP-S+), nonsmoker periodontitis patients (CP+S-) and smoker periodontitis patients (CP+S+). Clinical periodontal parameters were recorded before taking the GCF and saliva samples. 8-OHdG, MLT and MPO levels were measured biochemically.
Results: The lowest 8-OHdG and MPO levels were recorded in the (CP-S-) group (p<0.05). 8-OHdG and MPO levels were statistically higher in (CP-S+) group than (CP-S-) group (p<0.05). MLT levels in saliva were statistically higher in periodontally healthy (CP-) groups than CP groups (p<0.05).
Conclusions: CP causes a significant difference on the parameters connected with oxidative stress. For periodontally healthy applicants, smoking leads to statistically significant changes on the parameters in general; while the effect of smoking to the parameters was found insignificant in the CP groups. Findings of this study reveal the role of smoking on the development of periodontitis, due to negatively affecting the parameters linked to oxidative stress, that are crucial in the patogenesis of periodontal diseases.
KEY WORDS: 8-hydroxy-2´-deoxyguanosine (8-OHdG), chronic periodontitis (CP), melatonin (MLT), myeloperoxidase (MPO), oxidative stres (OS), smoking, tobacco.

Kronik Periodontitis Ve Sigara Kullananımının Oksidatif Durum Üzerine Olan Etkisinin Değerlendirilmesi
Öz
Amaç: Bu çalışmanın amacı; kronik periodontitisli ve periodontal açıdan sağlıklı bireylerin DOS ve tükürük numunelerinde sigaranın MLT, MPO ve 8-OHdG düzeyleri üzerine muhtemel etkilerinin değerlendirilmesidir.
Gereç ve yöntem : Çalışma protokolü gereği her biri 15 katılımcıdan oluşan 4 grup oluşturuldu; periodontal olarak sağlıklı ve sigara içemeyen grup (P-S-), periodontal olarak sağlıklı ve sigara içen grup (P-S+), kronik periodontitisli ve sigara içmeyen grup (P+S-) ile kronik periodontitisli ve sigara içen grup (P+S+). DOS ve tükürük örnekleri alınmadan önce klinik periodontal parametreler kaydedildi. MLT, MPO ve 8-OHdG düzeyleri biyokimyasal ve olarak ölçüldü.
Bulgular : En yüksek MPO ve 8-OHdGdüzeyleri ile en düşük MLT düzeyleri (P+S+) grubunda tespit edildi. En yüksek MLT düzeyi ise (P-S-) grubunda gözlendi. Sigara kullanımı ve kronik periodontitisin biyokimyasal ve histopatolojik parametreleri benzer şekilde etkilediği tespit edildi.
Sonuç : KP’nin oksidatif stres ile ilişkili parametrelerde anlamlı düzeyde farklılık oluşturduğu gözlendi. Sigara kullanımının genel olarak periodontal sağlıklı bireylerde parametrelerde belirgin bir değişime yol açtığı, periodontitisli gruplarda ise bu değişimin istatistiksel olarak anlamlı olmadığı görüldü. Bulgularımız, sigara içmenin periodontal hastalıkların patogenezinde rol alan oksitatif parametreleri olumsuz yönde etkileyerek periodontitis gelişiminde rol oynayabileceğini göstermektedir.
Anahtar Kelimeler: Isı şok proteinleri 60-70, kronik periodontitis, melatonin, myeloperoksidaz, oksitatif stress, sigara, 8-hydroxy-2'-deoxyguanosine.

Keywords

8-hydroxy-2´-deoxyguanosine (8-OHdG), chronic periodontitis (CP)

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