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EVALUATİON OF OXİDATİVE STATUS İN PATİENTS WİTH CHRONİC PERİODONTİTİS AND ADDİTİONAL TOBACCO ABUSE: A CROSS-SECTİONAL STUDY

Year 2020, Volume: 30 Issue: 4, 536 - 544, 15.10.2020
https://doi.org/10.17567/ataunidfd.778795

Abstract

Aim: The aim of the present study was to investigate the 8-hydroxy-2´deoxyguanosine (8-OHdG) and melatonin (MLT) levels in saliva as well as myeloperoxidase (MPO) levels in gingival clevicular fluid (GCF) in smoker and nonsmoker chronic periodontitis (CP) patients and periodontally healthy individuals.
Methods: 4 groups of 15 applicants were formed in accordance with the research protocole as follows; periodontally healthy nonsmokers (CP-S-), periodontally healthy smokers (CP-S+), nonsmoker periodontitis patients (CP+S-) and smoker periodontitis patients (CP+S+). Clinical periodontal parameters were recorded before taking the GCF and saliva samples. 8-OHdG, MLT and MPO levels were measured biochemically.
Results: The lowest 8-OHdG and MPO levels were recorded in the (CP-S-) group (p<0.05). 8-OHdG and MPO levels were statistically higher in (CP-S+) group than (CP-S-) group (p<0.05). MLT levels in saliva were statistically higher in periodontally healthy (CP-) groups than CP groups (p<0.05).
Conclusions: CP causes a significant difference on the parameters connected with oxidative stress. For periodontally healthy applicants, smoking leads to statistically significant changes on the parameters in general; while the effect of smoking to the parameters was found insignificant in the CP groups. Findings of this study reveal the role of smoking on the development of periodontitis, due to negatively affecting the parameters linked to oxidative stress, that are crucial in the patogenesis of periodontal diseases.
KEY WORDS: 8-hydroxy-2´-deoxyguanosine (8-OHdG), chronic periodontitis (CP), melatonin (MLT), myeloperoxidase (MPO), oxidative stres (OS), smoking, tobacco.

Kronik Periodontitis Ve Sigara Kullananımının Oksidatif Durum Üzerine Olan Etkisinin Değerlendirilmesi
Öz
Amaç: Bu çalışmanın amacı; kronik periodontitisli ve periodontal açıdan sağlıklı bireylerin DOS ve tükürük numunelerinde sigaranın MLT, MPO ve 8-OHdG düzeyleri üzerine muhtemel etkilerinin değerlendirilmesidir.
Gereç ve yöntem : Çalışma protokolü gereği her biri 15 katılımcıdan oluşan 4 grup oluşturuldu; periodontal olarak sağlıklı ve sigara içemeyen grup (P-S-), periodontal olarak sağlıklı ve sigara içen grup (P-S+), kronik periodontitisli ve sigara içmeyen grup (P+S-) ile kronik periodontitisli ve sigara içen grup (P+S+). DOS ve tükürük örnekleri alınmadan önce klinik periodontal parametreler kaydedildi. MLT, MPO ve 8-OHdG düzeyleri biyokimyasal ve olarak ölçüldü.
Bulgular : En yüksek MPO ve 8-OHdGdüzeyleri ile en düşük MLT düzeyleri (P+S+) grubunda tespit edildi. En yüksek MLT düzeyi ise (P-S-) grubunda gözlendi. Sigara kullanımı ve kronik periodontitisin biyokimyasal ve histopatolojik parametreleri benzer şekilde etkilediği tespit edildi.
Sonuç : KP’nin oksidatif stres ile ilişkili parametrelerde anlamlı düzeyde farklılık oluşturduğu gözlendi. Sigara kullanımının genel olarak periodontal sağlıklı bireylerde parametrelerde belirgin bir değişime yol açtığı, periodontitisli gruplarda ise bu değişimin istatistiksel olarak anlamlı olmadığı görüldü. Bulgularımız, sigara içmenin periodontal hastalıkların patogenezinde rol alan oksitatif parametreleri olumsuz yönde etkileyerek periodontitis gelişiminde rol oynayabileceğini göstermektedir.
Anahtar Kelimeler: Isı şok proteinleri 60-70, kronik periodontitis, melatonin, myeloperoksidaz, oksitatif stress, sigara, 8-hydroxy-2'-deoxyguanosine.

References

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EVALUATION OF THE EFFECT OF CHRONIC PERIODONTITIS AND ADDITIONAL TOBACCO ABUSE ON OXIDATIVE STATUS : A CROSS-SECTIONAL STUDY

Year 2020, Volume: 30 Issue: 4, 536 - 544, 15.10.2020
https://doi.org/10.17567/ataunidfd.778795

Abstract

Aim: The aim of the present study was to investigate the 8-hydroxy-2´deoxyguanosine (8-OHdG) and melatonin (MLT) levels in saliva as well as myeloperoxidase (MPO) levels in gingival clevicular fluid (GCF) in smoker and nonsmoker chronic periodontitis (CP) patients and periodontally healthy individuals.
Methods: 4 groups of 15 applicants were formed in accordance with the research protocole as follows; periodontally healthy nonsmokers (CP-S-), periodontally healthy smokers (CP-S+), nonsmoker periodontitis patients (CP+S-) and smoker periodontitis patients (CP+S+). Clinical periodontal parameters were recorded before taking the GCF and saliva samples. 8-OHdG, MLT and MPO levels were measured biochemically.
Results: The lowest 8-OHdG and MPO levels were recorded in the (CP-S-) group (p<0.05). 8-OHdG and MPO levels were statistically higher in (CP-S+) group than (CP-S-) group (p<0.05). MLT levels in saliva were statistically higher in periodontally healthy (CP-) groups than CP groups (p<0.05).
Conclusions: CP causes a significant difference on the parameters connected with oxidative stress. For periodontally healthy applicants, smoking leads to statistically significant changes on the parameters in general; while the effect of smoking to the parameters was found insignificant in the CP groups. Findings of this study reveal the role of smoking on the development of periodontitis, due to negatively affecting the parameters linked to oxidative stress, that are crucial in the patogenesis of periodontal diseases.
KEY WORDS: 8-hydroxy-2´-deoxyguanosine (8-OHdG), chronic periodontitis (CP), melatonin (MLT), myeloperoxidase (MPO), oxidative stres (OS), smoking, tobacco.

Kronik Periodontitis Ve Sigara Kullananımının Oksidatif Durum Üzerine Olan Etkisinin Değerlendirilmesi
Öz
Amaç: Bu çalışmanın amacı; kronik periodontitisli ve periodontal açıdan sağlıklı bireylerin DOS ve tükürük numunelerinde sigaranın MLT, MPO ve 8-OHdG düzeyleri üzerine muhtemel etkilerinin değerlendirilmesidir.
Gereç ve yöntem : Çalışma protokolü gereği her biri 15 katılımcıdan oluşan 4 grup oluşturuldu; periodontal olarak sağlıklı ve sigara içemeyen grup (P-S-), periodontal olarak sağlıklı ve sigara içen grup (P-S+), kronik periodontitisli ve sigara içmeyen grup (P+S-) ile kronik periodontitisli ve sigara içen grup (P+S+). DOS ve tükürük örnekleri alınmadan önce klinik periodontal parametreler kaydedildi. MLT, MPO ve 8-OHdG düzeyleri biyokimyasal ve olarak ölçüldü.
Bulgular : En yüksek MPO ve 8-OHdGdüzeyleri ile en düşük MLT düzeyleri (P+S+) grubunda tespit edildi. En yüksek MLT düzeyi ise (P-S-) grubunda gözlendi. Sigara kullanımı ve kronik periodontitisin biyokimyasal ve histopatolojik parametreleri benzer şekilde etkilediği tespit edildi.
Sonuç : KP’nin oksidatif stres ile ilişkili parametrelerde anlamlı düzeyde farklılık oluşturduğu gözlendi. Sigara kullanımının genel olarak periodontal sağlıklı bireylerde parametrelerde belirgin bir değişime yol açtığı, periodontitisli gruplarda ise bu değişimin istatistiksel olarak anlamlı olmadığı görüldü. Bulgularımız, sigara içmenin periodontal hastalıkların patogenezinde rol alan oksitatif parametreleri olumsuz yönde etkileyerek periodontitis gelişiminde rol oynayabileceğini göstermektedir.
Anahtar Kelimeler: Isı şok proteinleri 60-70, kronik periodontitis, melatonin, myeloperoksidaz, oksitatif stress, sigara, 8-hydroxy-2'-deoxyguanosine.

References

  • 1. Brown LJ, Loe H. Prevalence, extent, severity and progression of periodontal disease. Periodontology 2000. 1993;2:57-71. Epub 1993/06/01.
  • 2. Periodontology. TAao. The pathogenesis of periodontal diseases (position paper). Journal of Periodontology. 1999;70:457-70.
  • 3. Gasner NS, Schure RS. Periodontal Disease. StatPearls. Treasure Island (FL)2020.
  • 4. Chapple IL, Matthews JB. The role of reactive oxygen and antioxidant species in periodontal tissue destruction. Periodontology 2000. 2007;43:160-232. Epub 2007/01/12.
  • 5. Martinez-Herrera M, Abad-Jimenez Z, Silvestre FJ, Lopez-Domenech S, Marquez-Arrico CF, Silvestre-Rangil J, et al. Effect of Non-Surgical Periodontal Treatment on Oxidative Stress Markers in Leukocytes and Their Interaction with the Endothelium in Obese Subjects with Periodontitis: A Pilot Study. Journal of clinical medicine. 2020;9(7). Epub 2020/07/09.
  • 6. Tonetti MS. Cigarette smoking and periodontal diseases: etiology and management of disease. Annals of periodontology. 1998;3(1):88-101. Epub 1998/09/02.
  • 7. Fredriksson MI, Figueredo CM, Gustafsson A, Bergstrom KG, Asman BE. Effect of periodontitis and smoking on blood leukocytes and acute-phase proteins. J Periodontol. 1999;70(11):1355-60. Epub 1999/12/10.
  • 8. Periodontology AAo. Tobacco use and the periodontal patient (position paper). Journal of Periodontology. 1996;67:51-6.
  • 9. Bergstrom J. Tobacco smoking and chronic destructive periodontal disease. Odontology. 2004;92(1):1-8. Epub 2004/10/19.
  • 10. Lu X, Cai J, Kong H, Wu M, Hua R, Zhao M, et al. Analysis of cigarette smoke condensates by comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry I acidic fraction. Analytical chemistry. 2003;75(17):4441-51. Epub 2003/11/25.
  • 11. Guentsch A, Preshaw PM, Bremer-Streck S, Klinger G, Glockmann E, Sigusch BW. Lipid peroxidation and antioxidant activity in saliva of periodontitis patients: effect of smoking and periodontal treatment. Clinical oral investigations. 2008;12(4):345-52. Epub 2008/05/30.
  • 12. Canakci CF, Cicek Y, Canakci V. Reactive oxygen species and human inflammatory periodontal diseases. Biochemistry Biokhimiia. 2005;70(6):619-28. Epub 2005/07/26.
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There are 70 citations in total.

Details

Primary Language English
Subjects Dentistry
Journal Section Araştırma Makalesi
Authors

Didem Özkal Eminoğlu This is me 0000-0001-9406-3368

Varol Çanakçı This is me 0000-0002-5314-0889

Publication Date October 15, 2020
Published in Issue Year 2020 Volume: 30 Issue: 4

Cite

APA Özkal Eminoğlu, D., & Çanakçı, V. (2020). EVALUATİON OF OXİDATİVE STATUS İN PATİENTS WİTH CHRONİC PERİODONTİTİS AND ADDİTİONAL TOBACCO ABUSE: A CROSS-SECTİONAL STUDY. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi, 30(4), 536-544. https://doi.org/10.17567/ataunidfd.778795
AMA Özkal Eminoğlu D, Çanakçı V. EVALUATİON OF OXİDATİVE STATUS İN PATİENTS WİTH CHRONİC PERİODONTİTİS AND ADDİTİONAL TOBACCO ABUSE: A CROSS-SECTİONAL STUDY. Ata Diş Hek Fak Derg. October 2020;30(4):536-544. doi:10.17567/ataunidfd.778795
Chicago Özkal Eminoğlu, Didem, and Varol Çanakçı. “EVALUATİON OF OXİDATİVE STATUS İN PATİENTS WİTH CHRONİC PERİODONTİTİS AND ADDİTİONAL TOBACCO ABUSE: A CROSS-SECTİONAL STUDY”. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi 30, no. 4 (October 2020): 536-44. https://doi.org/10.17567/ataunidfd.778795.
EndNote Özkal Eminoğlu D, Çanakçı V (October 1, 2020) EVALUATİON OF OXİDATİVE STATUS İN PATİENTS WİTH CHRONİC PERİODONTİTİS AND ADDİTİONAL TOBACCO ABUSE: A CROSS-SECTİONAL STUDY. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi 30 4 536–544.
IEEE D. Özkal Eminoğlu and V. Çanakçı, “EVALUATİON OF OXİDATİVE STATUS İN PATİENTS WİTH CHRONİC PERİODONTİTİS AND ADDİTİONAL TOBACCO ABUSE: A CROSS-SECTİONAL STUDY”, Ata Diş Hek Fak Derg, vol. 30, no. 4, pp. 536–544, 2020, doi: 10.17567/ataunidfd.778795.
ISNAD Özkal Eminoğlu, Didem - Çanakçı, Varol. “EVALUATİON OF OXİDATİVE STATUS İN PATİENTS WİTH CHRONİC PERİODONTİTİS AND ADDİTİONAL TOBACCO ABUSE: A CROSS-SECTİONAL STUDY”. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi 30/4 (October 2020), 536-544. https://doi.org/10.17567/ataunidfd.778795.
JAMA Özkal Eminoğlu D, Çanakçı V. EVALUATİON OF OXİDATİVE STATUS İN PATİENTS WİTH CHRONİC PERİODONTİTİS AND ADDİTİONAL TOBACCO ABUSE: A CROSS-SECTİONAL STUDY. Ata Diş Hek Fak Derg. 2020;30:536–544.
MLA Özkal Eminoğlu, Didem and Varol Çanakçı. “EVALUATİON OF OXİDATİVE STATUS İN PATİENTS WİTH CHRONİC PERİODONTİTİS AND ADDİTİONAL TOBACCO ABUSE: A CROSS-SECTİONAL STUDY”. Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi, vol. 30, no. 4, 2020, pp. 536-44, doi:10.17567/ataunidfd.778795.
Vancouver Özkal Eminoğlu D, Çanakçı V. EVALUATİON OF OXİDATİVE STATUS İN PATİENTS WİTH CHRONİC PERİODONTİTİS AND ADDİTİONAL TOBACCO ABUSE: A CROSS-SECTİONAL STUDY. Ata Diş Hek Fak Derg. 2020;30(4):536-44.

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