Sığırların Infeksiyöz Keratokonjuktivitis Hastalığının Seroprevalansının ELISA ve AGID Testi ile Belirlenmesi

Abstract

Sığırların infeksiyöz keratokonjunktivitis hastalığı (IBK) sığır kornea ve konjunktivasının çok bulaşıcı bir infeksiyöz hastalığı olup sığır yetiştiriciliğinde önemli ekonomik kayıplara neden olmaktadır. Bu çalışmada, ELISA ve AGID ile sığır serumlarında M. bovis’e karşı oluşmuş serum antikorlarının belirlenmesi ve IBK’nın seroprevalansı ile ilgili bilgi edinilmesi amaçlandı. Araştırmada Türkiye’deki çeşitli çiftliklerdeki sığırlardan temin edilen toplam 552 kan serumu Moraxella bovis (M. bovis)’e karşı oluşmuş spesifik antikorlar yönünden teste tabi tutuldu. Klinik bulgu gösteren hayvanlara ait serumların %26.1’ i ELISA, %5.8’ i ise Agar jel immünodifüzyon testi ile pozitif tespit edildi. Sağlıklı görünen hayvanlara ait serumların ise %26.3’ü ELISA, %5.6’sı Agar jel immünodifüzyon testi ile pozitif olarak tespit edildi. Sonuçlara göre en yüksek seropozitiflik 12 ila 24 aylık sığırlarda (%36.5) saptandı. Klinik bulgu gösteren ve klinik bulgu göstermeyen sığırlarda seroprevalans oranlarının benzer olduğu tespit edildi. Keratokonjunktivit gibi klinik bulgular, spesifik olarak IBK’nın sürüdeki gerçek oranı hakkında bilgi vermemektedir. Bu nedenle etkenin izolasyon ve identifikasyonunun yapılmasının yanısıra hastalığın sürüdeki varlığının ve yaygınlığının tespiti için tarama amacıyla ELISA gibi serolojik bir test tercih edilebilir.

Keywords

• Moraxella bovis
• Agar jel immünodiffüzyon
• ELISA
• Seroprevalans
• Türkiye

Determination of Seroprevalence of Infectious Bovine Keratoconjunctivitis Disease by ELISA and AGID

Abstract

Infectious Bovine Keratoconjunctivitis (IBK) is a highly contagious disease of conjunctiva and cornea in cattle which causes significant economic losses in cattle breeding. The objective of this study is to determine serum antibodies against M. bovis and obtaining information about the seroprevalence of IBK by AGID test and ELISA. Serum samples, collected from 552 cattle in different herds in Turkey were examined for specific antibodies against Moraxella bovis (M. bovis). The seropositivity of IBK in cattle sera with clinical signs was determined as 26.1% and 5.8% by ELISA and AGID, respectively. Seropositivity in sera samples of healthy appeared cattle were detected as 26.3% and 5.6% by ELISA and AGID, respectively. According to the results, the highest seropositivity (36.5%) was detected in the animals aged 12-24 months. Similar seroprevalence detection rates were recorded in cattle with and without clinical signs. Clinical signs, spesifically, does not give knowledge about the exact percentage of the IBK. Therefore, besides isolation and identification of the agent, serological tests like ELISA, as a screening test, should be preferred to determine the presence and spread of IBK in the herd.

Keywords

• Agar gel immunodiffusion
• ELISA
• Moraxella bovis
• Seroprevalence
• Turkey

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