Seropositivity of Mycobacterium paratuberculosis in Cattle with Chronic Diarrhea in the Middle Black Sea Region

Year 2018, Volume: 13 Issue: 1, 1 - 5, 25.04.2018

Mehmet Tutuncu , Yunus Kilicoglu Murat Güzel , Didem Pekmezci , Timur Gulhan

https://doi.org/10.17094/ataunivbd.297128

Cited By: 2

Abstract

Paratuberculosis (Johne's disease), caused by Mycobacterium avium subsp. paratuberculosis (MAP) is one of the most common and contagious infections of farm animals mainly cattle herds. MAP leads to a chronic and progressive enteric disease that results poor body condition, reduced milk production, premature culling, and decimate slaughter value in cattle industry. The aim of this study was to investigate of the seropositivity of MAP in cattle with chronic diarrhea in Middle Black Sea Region. For this purpose, a total of 859 Holstein-Friesian dairy cattle (˃2 years old) from 15 herds with companied to chronic diarrhea were examined in Amasya and Samsun provinces, between 2012 and 2014. MAP antibodies were determined by indirect commercial enzyme-linked immunosorbent assay ELISA (iELISA) kit. Of the 859 samples, 86 (10.0%) and of 15 herds, 7 (46.7%) were positive for the MAP antibodies. In 2012, 2013 and 2014, positive rates of 2.4%, 21.5% and 50% were detected, respectively. As a result, there was a significant increase in MAP seropositivity in chronic diarrheal cattle. It is thought that the results of the study will contribute to the development of effective control programs for eradication of the disease and clinicians working in the region will benefit from the attention of veterinarians.

Keywords

Cattle, İELISA, Diarrhea, Johne’s disease, Paratuberculosis

Orta Karadeniz Bölgesinde Kronik İshalli Sığırlarda Mycobacterium paratuberculosis’in Seropozitifliği

Abstract

Paratüberküloz (Johne’s hastalığı), Mycobacterium avium subsp. paratuberculosis (MAP) tarafından oluşturulan, başta sığır sürüleri olmak üzere çiftlik hayvanlarının en yaygın ve bulaşıcı enfeksiyonlarından birisidir. MAP sığırlarda kronik ve ilerleyici bir enterik hastalığa neden olarak vücut kondüsyon kaybı, düşük süt verimi, erken kesim ve düşük kesim değerine neden olan bir hastalıktır. Bu çalışmada, orta Karadeniz bölgesindeki kronik ishalli şikayeti olan sığırlarda MAP antikor prevalansının tespiti amaçlandı. Bu amaçla 2012-2014 yılları arasında Amasya ve Samsun illerindeki 15 işletmeden 2 yaş ve üzeri kronik ishalli 859 Holstein-Friesian süt sığırı incelendi. MAP antikorları ticari bir indirekt ELISA kiti (iELISA) ile belirlendi. İncelenen serum örneklerinin 86’sı (%10,0) ve işletmelerin 7’si (%46,7) MAP antikorları yönünden pozitif olarak bulundu. 2012, 2013 ve 2014 yıllarında sırasıyla %2,4, %21,5 ve %50,0 oranında pozitiflik saptandı. Sonuç olarak, kronik ishalli sığırlarda MAP seropozitifliği bakımından belirgin bir artış olduğu görüldü. Çalışma sonuçlarının bölgede görev yapan klinisyen veteriner hekimlerin dikkatini çekmek açısından faydalı olacağı ve hastalığın eredikasyonu için etkin kontrol programlarının geliştirilmesi yönünde önemli katkılar yapacağı düşünülmektedir.

 

Keywords

iELISA, İshal, Johne’s hastalığı, Paratüberküloz, Sığır

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