Abstract
Bu çalışmada, Kars yöresinde halk elinde yetiştirilen kaz işletmelerinden kaz kesimi esnasında alınan kan serum örneklerinde Salmonella Enteritidis antikorlarının Enzyme-Linked Immunosorbent Assay (ELISA) yönetimi ile araştırılması amaçlanmıştır. Bu amaçla, Kars il merkezinde 8 ve Arpalı köyünde 2 kaz işletmesinden olmak üzere toplam 10 farklı işletmeden ve önceden S. Enteritidis ve diğer Salmonella enfeksiyonlarına karşı herhangi bir aşı geçmişi olmayan 1 yaşındaki 315 adet erkek ve dişi yerli kazdan örnek alındı. İncelenen 315 kaz kan serum örneğinin 16 (%5.07)’sı S. Enteritidis antikorları yönünden pozitif, 48 (%15.23)’i şüpheli ve 251 (%79.68)’i negatif saptandı. En yüksek S. Enteritidis antikor pozitifliği Kars il merkezindeki işletme 7 ile Arpalı köyündeki işletme 10’da (sırasıyla %16.66 ve %12.5) saptandı ve işletmeler arası bu pozitiflik istatistiksel olarak anlamlı (P<0.01) bulundu. Kars ile merkezine (%4.16) oranla Arpalı köyü işletmelerine ait S. Enteritidis antikor pozitifliği (%8) daha yüksek saptanırken, bu iki lokasyona ait işletmeler arası anlamlı fark belirlenememiştir (P>0.05). Kars yöresinde kazlarda S. Enteritidis’in varlığı ve yaygınlığı üzerine yapılan bu çalışmada, kaz kan serumu örneklerinden %5.07 oranında antikor pozitiflik saptanmasının önemli olduğu; bununla birlikte vertikal bulaşma özelliğine sahip olan ve karkas kontaminasyonunun yaygın olduğu Salmonella enfeksiyonlarında benzer tarama testleri yapılmasının hastalığın prevalansının azaltılmasına fayda sağlayacağı düşünülmektedir.
Keywords
References
- 1. Rahman HS., Othman HH., 2017. Salmonella Infection: The common cause of human food poisoning. Prog Biosci Bioeng, 1, 5-10. 2. Brito DAP., Sousa GLA., de Souza YL., Reis V., de Sousa Silva JR., Reis A., Oba A., 2019. Sources of paratyphoid Salmonella in the production chain of broilers in the Northern mesoregion of Maranhão State, Brazil. Vet Med, 40, 3021-3034. 3. Otlu S., 2016. Kazlarda Enfeksiyöz Hastlıklar. Turkiye Klinikleri J Reprod Artif Insemin-Special Topics, 2, 56-65. 4. Nicholas RAJ., Cullen GA., 1991. Development and application of an ELISA for detecting antibodies to Salmonella Enteritidis in chicken flocks. Vet Rec, 128, 74-76. 5. Furrer B., Baumgartner A., Bommeli W., 1993. Enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against Salmonella Enteritidis in chicken blood or egg yolk. Zentralbl Bakteriol, 279, 191-200. 6. Barrow PA., 1994. Use of ELISAs for monitoring Salmonella in poultry. Vet Rec, 22, 99. 7. Arslan C., 2013. Kaz Besleme ve Yetiştiriciliği. 2. Baskı, Ss: 1-130, Medipres Matbaacılık Yayıncılık, Malatya. 8. Tilki M., Saatcı M., 2013. Her Yönüyle Kaz Yetiştiriciliği. 1. Baskı, Ss: 1-131, Salmat Basım Yayıncılık, Ankara. 9. Carlı T., Caner V., Eyigör A., 2001. Prevalance of Salmonella serovars in chickens in Turkey. J Food Prot, 64, 1832-1835. 10. Goncagul G., Gunaydın E., Carlı KT., 2005. Prevalence of Salmonella serogroups in chicken meat. Turk J Vet Anim Sci, 29, 103-106. 11. Cui M., Xie M., Qu Z., Zhao S., Wang J., Wang Y., He T., Wang H., Zuo Z., Wu C., 2016. Prevalence and antimicrobial resistance of Salmonella isolated from an integrated broiler chicken supply chain in Qingdao, China. Food Control, 62, 270-276. 12. Genç O., Otlu S., 2005. Salmonella isolations from different avian species in Kars district of Turkey. Kafkas Univ Vet Fak Derg, 11, 25-27. 13. Pan ZM., Geng SZ., Zhou YQ., Liu ZY., Fang Q., Liu BB., Jiao XA., 2010. Prevalence and antimicrobial resistance of Salmonella sp. isolated from domestic animals in Eastern China. J Anim Vet Adv, 9, 2290-2294. 14. Jamali H., Radmehr B., Ismail S., 2014. Prevalence and antimicrobial resistance of Listeria, Salmonella, and Yersinia species isolates in ducks and geese. Poult Sci J, 93, 1023-1030. 15. Ata Z., Temelli S., Eyigör A., Çarlı KT., 2017. Salmonella Enteritidis predominance determined by serotyping and real-time PCR in poultry-derived food and avian isolates. Turk J Vet Anim Sci, 41, 187-192. 16. Whyte P., McGill K., Collins JD., Gormley E., 2002. The prevalence and PCR detection of Salmonella contamination in raw poultry. Vet Microbiol, 89, 53-60. 17. Rodrigue DC., Tauxe RV., Roe B., 1990. International increase in Salmonella Enteritidis: A new pandemic? Epidemiol Infect, 105, 21-27. 18. Aksakal A., 2003. Bazı kanatlıların dışkılarında Salmonella türlerinin varlığı ve yaygınlığı ile antibiyotik duyarlılıkları. YYU Vet Fak Derg, 14, 95-101. 19. Trawinska B., Saba L., Wdowiak L., Ondrasovicova O., Nowakowicz-Debek B., 2008. Evaluation of Salmonella rod incidence in poultry in the Lublin Province over the years 2001-2005. Ann Agr Env Med, 15, 131-134. 20. Ibrahim HM,. Sayed RH,. Abdel-Aziz WR,. Soliman RT., 2017. Preparation and evaluation of Salmonella Enteritidis antigen conjugated with nanogold for screening of poultry flocks. Vet World, 10, 848-853. 21. Cooper GL., Nicholas RAJ., Bracewell CD., 1989. Serological and bacteriological investigations of chickens from flocks naturally infected with Salmonella Enteritidis. Vet Rec, 125, 567-572. 22. Chart H., Rowe B., Baskerville A., Humphrey TJ., 1990. Serological response of chickens to Salmonella Enteritidis infection. Epidemiol Infect, 104, 63-71. 23. De Jong WA., 1993. Salmonella Enteritidis eradication programme in The Netherlands. Proceedings of the EC Workshop on ELISAs for serological detection of Salmonella in poultry. 7-9 June, Brussels. 24. Dahal N., 2007. Prevalence and antimicrobial resistance of Salmonella in imported chicken carcasses in Bhutan. Master Thesis, Chiang Mai University And Free University, Berlin. 25. Mirhosseini SA., Fooladi AAI., Amani F., Sedighian H., 2017. Production of recombinant flagellin to develop ELISA-based detection of Salmonella Enteritidis. Brazilian J Microbiol, 48, 774-781.
Abstract
In this study, it was aimed to investigate Salmonella Enteritidis antibodies by Enzyme-Linked Immunosorbent Assay (ELISA) in blood serum samples obtained from the goose enterprises grown in Kars Region during the goose slaughtering period. For this purpose, a total of 10 different flocks, 8 from Kars Province center and 2 from Arpalı Village, were included in the study. Totally, one year old 315 female and male domestic geese which had no vaccination history against S. Enteritidis and other Salmonella infections were sampled. Of the 315 goose blood serum samples examined, 16 (5.07%) were positive, 48 (15.23%) were suspicious and 251 (79.68%) were negative for S. Enteritidis antibodies. The highest S. Enteritidis antibody positivity was detected in the Flock 7 in Kars city center and in the Flock 10 in Arpalı Village with a rate as 16.66% and 12.5%, respectively. This positivity between the flocks was statistically significant (P<0.01). While the S. Enteritidis antibody positivity (8%) of Arpalı Village was found higher than the Kars Province central has (4.16%), there was no significant difference between these two locations (P>0.05). In this study conducted on the presence and prevalence of S. enteritidis in geese in Kars province, it was found that 5.07% antibody positivity was considered to be important in geese blood serum samples; in addition to this, similar screening tests for Salmonella infections, which have vertical transmission characteristic and carcass contamination are common, are thought to be beneficial in decreasing the prevalence of the disease.
Keywords
References
- 1. Rahman HS., Othman HH., 2017. Salmonella Infection: The common cause of human food poisoning. Prog Biosci Bioeng, 1, 5-10. 2. Brito DAP., Sousa GLA., de Souza YL., Reis V., de Sousa Silva JR., Reis A., Oba A., 2019. Sources of paratyphoid Salmonella in the production chain of broilers in the Northern mesoregion of Maranhão State, Brazil. Vet Med, 40, 3021-3034. 3. Otlu S., 2016. Kazlarda Enfeksiyöz Hastlıklar. Turkiye Klinikleri J Reprod Artif Insemin-Special Topics, 2, 56-65. 4. Nicholas RAJ., Cullen GA., 1991. Development and application of an ELISA for detecting antibodies to Salmonella Enteritidis in chicken flocks. Vet Rec, 128, 74-76. 5. Furrer B., Baumgartner A., Bommeli W., 1993. Enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against Salmonella Enteritidis in chicken blood or egg yolk. Zentralbl Bakteriol, 279, 191-200. 6. Barrow PA., 1994. Use of ELISAs for monitoring Salmonella in poultry. Vet Rec, 22, 99. 7. Arslan C., 2013. Kaz Besleme ve Yetiştiriciliği. 2. Baskı, Ss: 1-130, Medipres Matbaacılık Yayıncılık, Malatya. 8. Tilki M., Saatcı M., 2013. Her Yönüyle Kaz Yetiştiriciliği. 1. Baskı, Ss: 1-131, Salmat Basım Yayıncılık, Ankara. 9. Carlı T., Caner V., Eyigör A., 2001. Prevalance of Salmonella serovars in chickens in Turkey. J Food Prot, 64, 1832-1835. 10. Goncagul G., Gunaydın E., Carlı KT., 2005. Prevalence of Salmonella serogroups in chicken meat. Turk J Vet Anim Sci, 29, 103-106. 11. Cui M., Xie M., Qu Z., Zhao S., Wang J., Wang Y., He T., Wang H., Zuo Z., Wu C., 2016. Prevalence and antimicrobial resistance of Salmonella isolated from an integrated broiler chicken supply chain in Qingdao, China. Food Control, 62, 270-276. 12. Genç O., Otlu S., 2005. Salmonella isolations from different avian species in Kars district of Turkey. Kafkas Univ Vet Fak Derg, 11, 25-27. 13. Pan ZM., Geng SZ., Zhou YQ., Liu ZY., Fang Q., Liu BB., Jiao XA., 2010. Prevalence and antimicrobial resistance of Salmonella sp. isolated from domestic animals in Eastern China. J Anim Vet Adv, 9, 2290-2294. 14. Jamali H., Radmehr B., Ismail S., 2014. Prevalence and antimicrobial resistance of Listeria, Salmonella, and Yersinia species isolates in ducks and geese. Poult Sci J, 93, 1023-1030. 15. Ata Z., Temelli S., Eyigör A., Çarlı KT., 2017. Salmonella Enteritidis predominance determined by serotyping and real-time PCR in poultry-derived food and avian isolates. Turk J Vet Anim Sci, 41, 187-192. 16. Whyte P., McGill K., Collins JD., Gormley E., 2002. The prevalence and PCR detection of Salmonella contamination in raw poultry. Vet Microbiol, 89, 53-60. 17. Rodrigue DC., Tauxe RV., Roe B., 1990. International increase in Salmonella Enteritidis: A new pandemic? Epidemiol Infect, 105, 21-27. 18. Aksakal A., 2003. Bazı kanatlıların dışkılarında Salmonella türlerinin varlığı ve yaygınlığı ile antibiyotik duyarlılıkları. YYU Vet Fak Derg, 14, 95-101. 19. Trawinska B., Saba L., Wdowiak L., Ondrasovicova O., Nowakowicz-Debek B., 2008. Evaluation of Salmonella rod incidence in poultry in the Lublin Province over the years 2001-2005. Ann Agr Env Med, 15, 131-134. 20. Ibrahim HM,. Sayed RH,. Abdel-Aziz WR,. Soliman RT., 2017. Preparation and evaluation of Salmonella Enteritidis antigen conjugated with nanogold for screening of poultry flocks. Vet World, 10, 848-853. 21. Cooper GL., Nicholas RAJ., Bracewell CD., 1989. Serological and bacteriological investigations of chickens from flocks naturally infected with Salmonella Enteritidis. Vet Rec, 125, 567-572. 22. Chart H., Rowe B., Baskerville A., Humphrey TJ., 1990. Serological response of chickens to Salmonella Enteritidis infection. Epidemiol Infect, 104, 63-71. 23. De Jong WA., 1993. Salmonella Enteritidis eradication programme in The Netherlands. Proceedings of the EC Workshop on ELISAs for serological detection of Salmonella in poultry. 7-9 June, Brussels. 24. Dahal N., 2007. Prevalence and antimicrobial resistance of Salmonella in imported chicken carcasses in Bhutan. Master Thesis, Chiang Mai University And Free University, Berlin. 25. Mirhosseini SA., Fooladi AAI., Amani F., Sedighian H., 2017. Production of recombinant flagellin to develop ELISA-based detection of Salmonella Enteritidis. Brazilian J Microbiol, 48, 774-781.
Details
| Primary Language | Turkish |
|---|---|
| Subjects | Health Care Administration |
| Journal Section | Araştırma Makaleleri |
| Authors | |
| Publication Date | December 25, 2019 |
| Published in Issue | Year 2019 Volume: 14 Issue: 3 |