Determination of Seroprevalence of Infectious Bovine Keratoconjunctivitis Disease by ELISA and AGID

Year 2020, Volume: 15 Issue: 2, 106 - 112, 27.10.2020

Lale Ataseven

https://doi.org/10.17094/ataunivbd.656701

Abstract

Infectious Bovine Keratoconjunctivitis (IBK) is a highly contagious disease of conjunctiva and cornea in cattle which causes significant economic losses in cattle breeding. The objective of this study is to determine serum antibodies against M. bovis and obtaining information about the seroprevalence of IBK by AGID test and ELISA. Serum samples, collected from 552 cattle in different herds in Turkey were examined for specific antibodies against Moraxella bovis (M. bovis). The seropositivity of IBK in cattle sera with clinical signs was determined as 26.1% and 5.8% by ELISA and AGID, respectively. Seropositivity in sera samples of healthy appeared cattle were detected as 26.3% and 5.6% by ELISA and AGID, respectively. According to the results, the highest seropositivity (36.5%) was detected in the animals aged 12-24 months. Similar seroprevalence detection rates were recorded in cattle with and without clinical signs. Clinical signs, spesifically, does not give knowledge about the exact percentage of the IBK. Therefore, besides isolation and identification of the agent, serological tests like ELISA, as a screening test, should be preferred to determine the presence and spread of IBK in the herd.

Keywords

Agar gel immunodiffusion, ELISA, Moraxella bovis, Seroprevalence, Turkey

Supporting Institution

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Project Number

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Thanks

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Sığırların Infeksiyöz Keratokonjuktivitis Hastalığının Seroprevalansının ELISA ve AGID Testi ile Belirlenmesi

Abstract

Sığırların infeksiyöz keratokonjunktivitis hastalığı (IBK) sığır kornea ve konjunktivasının çok bulaşıcı bir infeksiyöz hastalığı olup sığır yetiştiriciliğinde önemli ekonomik kayıplara neden olmaktadır. Bu çalışmada, ELISA ve AGID ile sığır serumlarında M. bovis’e karşı oluşmuş serum antikorlarının belirlenmesi ve IBK’nın seroprevalansı ile ilgili bilgi edinilmesi amaçlandı. Araştırmada Türkiye’deki çeşitli çiftliklerdeki sığırlardan temin edilen toplam 552 kan serumu Moraxella bovis (M. bovis)’e karşı oluşmuş spesifik antikorlar yönünden teste tabi tutuldu. Klinik bulgu gösteren hayvanlara ait serumların %26.1’ i ELISA, %5.8’ i ise Agar jel immünodifüzyon testi ile pozitif tespit edildi. Sağlıklı görünen hayvanlara ait serumların ise %26.3’ü ELISA, %5.6’sı Agar jel immünodifüzyon testi ile pozitif olarak tespit edildi. Sonuçlara göre en yüksek seropozitiflik 12 ila 24 aylık sığırlarda (%36.5) saptandı. Klinik bulgu gösteren ve klinik bulgu göstermeyen sığırlarda seroprevalans oranlarının benzer olduğu tespit edildi. Keratokonjunktivit gibi klinik bulgular, spesifik olarak IBK’nın sürüdeki gerçek oranı hakkında bilgi vermemektedir. Bu nedenle etkenin izolasyon ve identifikasyonunun yapılmasının yanısıra hastalığın sürüdeki varlığının ve yaygınlığının tespiti için tarama amacıyla ELISA gibi serolojik bir test tercih edilebilir.

Keywords

Moraxella bovis, Agar jel immünodiffüzyon, ELISA, Seroprevalans, Türkiye

Project Number

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References

• 1. Wilcox GE., 1968. Infectious bovine keratoconjunctivitis: A review. Vet Bull, 38, 349-359.
• 2. Baptista JHP., 1979. Infectious bovine keratoconjunctivitis. A review. Br Vet J, 135, 225-242.
• 3. Punch PI., Slatter DH., 1984. A review of infectious bovine keratoconjunctivitis. Vet Bull, 54, 193-207.
• 4. Singh S., Grice ID., Peak IR., Frost T., Yue G., 2018. The role of lipooligosaccaride in the biological activity of Moraxella bovis strains Epp636, Mb25 and L183/2, and isolation of capsular polysaccharide from L183/2. Carbohydr Res, 467,1-7.
• 5. Fonseca JC., Maia JMS., Varago FC., Gern JC., Carvalho WA., Silva SR., Mosqueira VCF., Brandao HM., Gulmarars AS., 2020. Cloxacillin nanostructured formulation for the treatment of bovine keratokoncunctivitis. Vet Anim Sci, 9, 100089.
• 6. Seid A., 2019. Review on Infectious Bovine Keratoconjunctivitis and its Economic Impacts in cattle. Dairy and Vet Sci J, 9, 1-8.
• 7. Gallagher CH., 1954. Investigation of the etiology of infectious ophthalmia of cattle. Aust Vet J, 30, 61-68.
• 8. Pugh GW., Hughes DE., 1968. Experimental bovine infectious keratoconjunctivitis caused by sunlampirradiation and Moraxella bovis infection: Correlation of hemolytic ability and pathogenicity. Am J Vet Res, 29, 835-839.
• 9. Holt JG., Krieg NR., Sneath PHA., Staley JT., Williams ST., 1994. Bergey’s Manual of Determinative Bacteriology. 9th ed., 90, Williams and Wilkins, Baltimore.
• 10. Robbins K., Dickey AM., Clawson ML., Loy JD., 2018. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry identification of Moraxella bovoculi and Moraxella bovis isolates from cattle. J Vet Diagn Invest, 30, 739-742.
• 11. Ely VL., Vorgos AC., Costa MM., Oliveira HP., Pötter L., Reghelin MA., Fernandes AW., Pereira DIB., Sangioni LA., Botton SA., 2019. Moraxella bovis, Moraxella ovis and Moraxella bovoculi; biofilm formation and lysozyme activity. J App Microbiol, 126, 369-376.
• 12. Dickey AM., Schuller G., Loy JD., Clawson ML., 2018. Whole genome sequencing of Moraxella bovoculi reveals high genetic diversity and evidence for interspecies recombination at multiple loci. PLOS One, 17, 12.
• 13. Arora AC., Killinger AH., Myers WL., 1976. Detection of Moraxella bovis antibodies in infectious bovine keratoconjunctivitis by a passive hemagglutination test. Am J Vet Res, 37, 1489-1492.
• 14. Killinger AH., Weisiger RM., Helper LC., Mansfield ME., 1978. Detection of Moraxella bovis antibodies in the sIgA, IgG and IgM classes of immunoglobulin in bovine lacrimal secretions by an indirect fluorescent antibody test. Am J Vet Res, 39, 931-934.
• 15. Bishop B., Schuring GG., Troutt HF., 1982. Enzyme-linked immunosorbent assay for the measurement of anti Moraxella bovis antibodies. Am J Vet Res, 43, 143-144.
• 16. Gil Turnes C., Araujo F., 1982. Characterization of strains of Moraxella bovis using double immunodiffusion. Can J Comp Med, 46, 165-168.
• 17. Maidment DC., Batty DL., 1986. Changes in titres of Moraxella bovis specific IgG in growing cattle. Br Vet J, 142, 65-69.
• 18. Formston C., 1954. Infectious keratoconjunctivitis of cattle (New Forest Disease). Vet Rec, 66, 522-527.
• 19. Slatter DH., Edwards MB., Hawkins CD., Wilcox GE., 1982. A national survey of occurence of infectious bovine keratoconjunctivitis. Aust Vet J, 59, 65-68.
• 20. Slatter DH., Edwards MB., Hawkins CD., Wilcox GE., 1982. A national survey of the clinical features, treatment and importance infectious bovine keratoconjunctivitis. Aust Vet J, 59, 69-72.
• 21. Makinde AA., Ezeh AO., Onoviran O., Umo I., 1985. Prevalance of serum antibodies to Moraxella bovis in cattle in Nigeria. Br Vet J, 141, 643-646.
• 22. Thakur DK., Shaheen JM., Roy BK., 1996. An outbreak of infections keratoconjunctivitis in caprine and bovine in Ranchi (Bihar). Indian Vet J, 73, 770-771.
• 23. Takele G., Zerihun A., 2000. Epidemiology of infectious keratoconjunctivits in cattle in south-east Ethiophia. J Vet Med A, 47, 169-173.
• 24. Erdeğer JE., 1989. Sığırlardan izole edilen Moraxella bovis suşlarının çeşitli özelliklerinin araştırılması. Ankara Üniversitesi Sağlık Bilimleri Enstitüsü, Türkiye.
• 25. Işık N., 2008. İnfeksiyöz bovine keratokonjuktivitisli sığırlarda Moraxella bovis'in izolasyonu ve antibiyotiklere duyarlılığının araştırılması. Dicle Üniv Vet Fak Derg, 1, 17-22.
• 26. Parin U., Kirkan Ş., Gumus S., 2017. Isolation of Moraxella bovis in cattle and detection of antibiotic susceptibilities. Int J Vet Sci, 6, 228-231. 27. Pugh GW., Hughes DE., Schultz VD., 1976. Experimentally induced infectious bovine keratoconjunctivitis: Resistance of vaccinated cattle to hemologous and heterologous strains of Moraxella bovis. Am J Vet Res, 37, 58-60.
• 28. Sümbüllüoğlu K., Sümbüllüoğlu V., 1997. Bioistatistik. 7th ed., 156-159, Hatiboğlu Yayınları, Ankara.
• 29. Tizard I., 1982. Veterinary Immunology. 2nd e.d., Chapter 9, W.B. Saunders, Philadelphia.
• 30. Pugh GW., Hughes DE., Packer RA., 1970. Bovine infectious keratoconjunctivitis interactions of Moraxella bovis and infectious bovine rhinotrachitis virus. Am J Vet Res, 31, 653-662.
• 31. Pugh GW., Hughes DE., 1975. Carrier state of Moraxella bovis and the development of preventive measures against disease. J Am Vet Med Assoc, 167, 310-313.
• 32. Smith DC., Grene WH., Allen JW., 1989. Antibodies related to resistance in bovine pink eye. Calif Vet, 34, 7-10.
• 33. Pugh GW., McDonald TJ., Booth GD., 1979. Infectious bovine keratoconjunctivitis. Influence of age on development of disease in vaccinated and non-vaccinated calves after exposure to Moraxella bovis. Am J Vet Res, 40, 762-766.
• 34. Dodt RM., 1977. The prevalance of bovine keratoconjunctivitis in a beef herd in northeastern Queensland. Aust Vet J, 53, 128-131.