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Turunçgil Ekstraktı Anti-giardial Terapötik Destek Sağlayabilir mi?

Year 2021, Volume 16, Issue 2, 149 - 154, 31.10.2021
https://doi.org/10.17094/ataunivbd.798450

Abstract

Bu araştırmada turunçgil ekstraktının, giardiasisli oğlaklardaki sağaltım etkinliğinin araştırılması amaçlandı. Bu kapsamda araştırmaya 12-68 günlük yaş aralığında Giardia duodenalis ile enfekte olduğu belirlenen 18 oğlak dahil edildi. Oğlaklar hiçbir ilaç uygulamasının yapılmadığı, kontrol grubu (n=8) ve 10 ml/hayvan başına dozda 7 gün boyunca turunçgil ekstraktının uygulandığı grup (n=10) olacak şekilde rastgele 2 gruba ayrıldı. Turunçgil ekstraktının anti-giardial terapötik etkisi, 0. ve 10. günlerdeki dışkının mikroskobik incelemesindeki ookist saçılımı ile belirlendi. Onuncu gündeki turunçgil ekstraktının terapötik etkinliğinin kontrol grubuna göre %99.9 olduğu ortaya çıkarıldı. Sonuç olarak ookist saçılımındaki kuvvetli azalma, turunçgil ekstraktının anti-giardiyal etkinliğini desteklemekte ve giardiazis sağaltımında alternatif olabileceğini göstermektedir.

References

  • 1. Pulina G., Milan MJ., Lavin MP., Theodoridis A., Morin E., Capote J., Caja G., 2018. Invited review: Current production trends, farm structures, and economics of the dairy sheep and goat sectors. J Dairy Sci, 101, 6715-6729.
  • 2. Singh DD., Pawaiya RVS., Gururaj K., Gangwar NK., Mishra AK., Singh R. Kumar A., 2018. Detection of Clostridium perfringens toxinotypes, enteropathogenic E. coli, Rota and corona viruses in the intestine of neonatal goat kids by molecular techniques. Indian J Anim Sci, 88, 655-661.
  • 3. Mishra AK., Singh DD., Kumar N., Kumarsen G., Paul S., Kumar A., 2020. Role of Bacterial and Parasitic Pathogens in Occurrence of Neonatal Diarrhoea in Goat-Kids. J Anim Res, 10, 389-395.
  • 4. Sargison ND., 2020. The critical importance of planned small ruminant livestock health and production in addressing global challenges surrounding food production and poverty alleviation. N Z Vet J, 68, 136-144.
  • 5. Toledo RDS., Martins FDC., Ferreira FP., de Almeida JC., Ogawa L., dos Santos HLE., Freire RL., 2017. Cryptosporidium spp. and Giardia spp. in feces and water and the associated exposure factors on dairy farms. PloS One, 12, e0175311.
  • 6. Platts-Mills JA., Babji S., Bodhidatta L., Gratz J., Haque R., Havt A., 2015. Pathogen-specific burdens of community diarrhoea in developing countries: a multisite birth cohort study (MAL-ED) Lancet Glob Health, 3, 564–e575.
  • 7. Savioli L., Smith H., Thompson A., 2006. Giardia and Cryptosporidium join the ‘neglected diseases initiative’ Trends Parasitol, 22, 203-208.
  • 8. Rossignol JF., 2010. Cryptosporidium and Giardia: treatment options and prospects for new drugs. Exp Parasitol, 124, 45-53.
  • 9. Alıç Ural D., Erdoğan H., Toplu S., Ayan A., 2017. Oğlaklarda Giardiazis Kontrolüne Yönelik Oral Klinoptilolit Uygulaması. Kocatepe Vet J, 10, 158-163.
  • 10. Riches A., Hart C., Trenholme K., Skinner-Adams TS., 2020. Anti-Giardia drug discovery: Current status and gut feelings. J Med Chem, 25, 13330-13354.
  • 11. Wu GA., Terol J., Ibanez V., Lopez-Garcia A., Perez-Roman E., Borreda C., Talon M., 2018. Genomics of the origin and evolution of Citrus. Nature, 554, 311-316.
  • 12. Shalaby NM., Abd-Alla HI., Ahmed HH., Basoudan N., 2011. Protective effect of Citrus sinensis and Citrus aurantifolia against osteoporosis and their phytochemical constituents. J Med Plants Res, 5, 579-588.
  • 13. Nidhi P., Rolta R., Kumar V., Dev K., Sourirajan A. 2020. Synergistic potential of Citrus aurantium L. essential oil with antibiotics against Candida albicans. J Ethnopharmacol, 262, 113-135.
  • 14. Falcinelli B., Famiani F., Paoletti A., D’Egidio S., Stagnari F., Galieni A., Benincasa, P., 2020. Phenolic Compounds and Antioxidant Activity of Sprouts from Seeds of Citrus Species. Agriculture, 10, 33.
  • 15. Okla MK., Alamri SA., Salem MZ., Ali HM., Behiry SI., Nasser RA., Soufan W., 2019. Yield, phytochemical constituents, and antibacterial activity of essential oils from the leaves/twigs, branches, branch wood, and branch bark of Sour Orange (Citrus aurantium L.). Processes, 7, 363.
  • 16. Ettebong E., Ubulom P., Etuk A., 2019. Antiplasmodial activity of methanol leaf extract of Citrus aurantifolia (Christm) Swingle. J Herbmed Pharmacol, 8, 274-280.
  • 17. Jimenez JC., Pinon A., Dive D., Capron M., Dei-Cas E., Convit J., 2009. Antibody response in children infected with Giardia intestinalis before and after treatment with Secnidazole. Am J Trop Med Hyg, 80, 11-15.
  • 18. Ural K., Aysul N., Voyvoda H., Ulutas B., Aldemir OS., Eren H,. 2014. Single dose of secnidazole treatment against naturally occuring Giardia duodenalis infection in Sakiz lambs. Rev MVZ Cordoba, 19, 4023-4032.
  • 19. Da Silva AS., Castro VS., Tonin AA., Brendler S., Costa MM., Jaques JA., Monteiro SG., 2011. Secnidazole for the treatment of giardiasis in naturally infected cats. Parasitol Int, 60, 429-432.
  • 20. Gultekin M., Ural K., Aysul N., Ayan A., Balikci C., Akyildiz G., 2016. The efficacy of chloroquine treatment of Giardia duodenalis infection in calves. Vlaams Diergeneeskd Tijdschr, 85, 335-341.
  • 21. Karademir U., Ural K., Aysul N., Ayan A., Toplu S., Ortlek O., Balιkci C., Kunyeli A., Erdogan H., 2016. The efficacy of chloroquine treatment against naturally occuring Giardia duodenalis infection in lambs. Rev MVZ Cordoba, 21, 5328-5335.
  • 22. Geurden T., Pohleb H., Sarrea C., Dreesena L., Vercruyssea J., Claerebouta E., 2011. The effcacy of a treatment with fenbendazole against anexperimental Giardia duodenalis infection in lambs. Small Rum Res, 96, 211-215.
  • 23. Ragbetli C., Tanritanir P., Yilmaz H., Yoruk I., Ozdemir H., 2014. Effect on biochemical parameters in naturally infected calves with giardiasis after treatment with albendazole Van Province, Turkey. Indian J Anim Res, 48, 38-44.
  • 24. Geurden T., Claerebout E., Dursin L., Deflandre A., Bernay F., Kaltsatos V., Vercruysse J., 2006. The efficacy of an oral treatment with paromomycin against an experimental infection with Giardia in calves. Vet Parasitol, 135, 241-247.
  • 25. Lalle M., Hanevik K., 2018. Treatment-refractory giardiasis: challenges and solutions. Infect Drug Resist, 11, 1921.
  • 26. Wood dos Santos T., Cristina Pereira Q., Teixeira L., Gambero A., A Villena J., Lima Ribeiro M., 2018. Effects of polyphenols on thermogenesis and mitochondrial biogenesis. Int J Mol Sci, 19, 2757.
  • 27. Paniagua M., Crespo J., Aris A., Devant M., 2019. Citrus aurantium flavonoid extract improves concentrate efficiency, animal behavior, and reduces rumen inflammation of Holstein bulls fed high-concentrate diets. Anim Feed Sci Tech, 258, 114304.
  • 28. Mayaa FAK., Alasadiy YDK., 2015. Effect of cold aqueous plant extract (Citrus aurantium and Solanum melongena) against the Giardia lamblia parasite and Entamoeba histolytica in vitro and measuring the LD50 for extracts in Al-Muthanna province. Res J Pharm Biol Chem Sci, 6, 1241-1252.
  • 29. Abdelqader A., Qarallah B., Al-Ramamneh D., Daş G., 2012. Anthelmintic effects of citrus peels ethanolic extracts against Ascaridia galli. Vet Parasitol, 188, 78-84.

May Citrus Extract Provide Anti-Giardial Therapeutic Efficacy?

Year 2021, Volume 16, Issue 2, 149 - 154, 31.10.2021
https://doi.org/10.17094/ataunivbd.798450

Abstract

In this study, it was aimed to investigate the therapeutic efficacy of the bitter orange extract in goat kids with giardiasis. A total of sixteen goat kids detected to be infected with Giardia duodenalis at the age of 12 to 68 days were enrolled in the study. Goat kids were randomly assigned into two groups, whereas goat kids in the control group (n= 8) without receiving any drug and group (n=10) received oral bitter orange extract at a dosage of 10 ml/goat kids for seven days. Anti-giardial therapeutic efficacy of bitter orange extract was determined by oocyst shedding interpreted with a microscopical examination of fecal samples on days 0 and 10. It was revealed that the therapeutic efficacy of orange bitter extract as compared with control was 99.9% on day 10. In conclusion, this significant decrease of oocyst shedding supports the anti-giardial activity of bitter orange extract and shows that it might be an alternative in the treatment of giardiasis.

References

  • 1. Pulina G., Milan MJ., Lavin MP., Theodoridis A., Morin E., Capote J., Caja G., 2018. Invited review: Current production trends, farm structures, and economics of the dairy sheep and goat sectors. J Dairy Sci, 101, 6715-6729.
  • 2. Singh DD., Pawaiya RVS., Gururaj K., Gangwar NK., Mishra AK., Singh R. Kumar A., 2018. Detection of Clostridium perfringens toxinotypes, enteropathogenic E. coli, Rota and corona viruses in the intestine of neonatal goat kids by molecular techniques. Indian J Anim Sci, 88, 655-661.
  • 3. Mishra AK., Singh DD., Kumar N., Kumarsen G., Paul S., Kumar A., 2020. Role of Bacterial and Parasitic Pathogens in Occurrence of Neonatal Diarrhoea in Goat-Kids. J Anim Res, 10, 389-395.
  • 4. Sargison ND., 2020. The critical importance of planned small ruminant livestock health and production in addressing global challenges surrounding food production and poverty alleviation. N Z Vet J, 68, 136-144.
  • 5. Toledo RDS., Martins FDC., Ferreira FP., de Almeida JC., Ogawa L., dos Santos HLE., Freire RL., 2017. Cryptosporidium spp. and Giardia spp. in feces and water and the associated exposure factors on dairy farms. PloS One, 12, e0175311.
  • 6. Platts-Mills JA., Babji S., Bodhidatta L., Gratz J., Haque R., Havt A., 2015. Pathogen-specific burdens of community diarrhoea in developing countries: a multisite birth cohort study (MAL-ED) Lancet Glob Health, 3, 564–e575.
  • 7. Savioli L., Smith H., Thompson A., 2006. Giardia and Cryptosporidium join the ‘neglected diseases initiative’ Trends Parasitol, 22, 203-208.
  • 8. Rossignol JF., 2010. Cryptosporidium and Giardia: treatment options and prospects for new drugs. Exp Parasitol, 124, 45-53.
  • 9. Alıç Ural D., Erdoğan H., Toplu S., Ayan A., 2017. Oğlaklarda Giardiazis Kontrolüne Yönelik Oral Klinoptilolit Uygulaması. Kocatepe Vet J, 10, 158-163.
  • 10. Riches A., Hart C., Trenholme K., Skinner-Adams TS., 2020. Anti-Giardia drug discovery: Current status and gut feelings. J Med Chem, 25, 13330-13354.
  • 11. Wu GA., Terol J., Ibanez V., Lopez-Garcia A., Perez-Roman E., Borreda C., Talon M., 2018. Genomics of the origin and evolution of Citrus. Nature, 554, 311-316.
  • 12. Shalaby NM., Abd-Alla HI., Ahmed HH., Basoudan N., 2011. Protective effect of Citrus sinensis and Citrus aurantifolia against osteoporosis and their phytochemical constituents. J Med Plants Res, 5, 579-588.
  • 13. Nidhi P., Rolta R., Kumar V., Dev K., Sourirajan A. 2020. Synergistic potential of Citrus aurantium L. essential oil with antibiotics against Candida albicans. J Ethnopharmacol, 262, 113-135.
  • 14. Falcinelli B., Famiani F., Paoletti A., D’Egidio S., Stagnari F., Galieni A., Benincasa, P., 2020. Phenolic Compounds and Antioxidant Activity of Sprouts from Seeds of Citrus Species. Agriculture, 10, 33.
  • 15. Okla MK., Alamri SA., Salem MZ., Ali HM., Behiry SI., Nasser RA., Soufan W., 2019. Yield, phytochemical constituents, and antibacterial activity of essential oils from the leaves/twigs, branches, branch wood, and branch bark of Sour Orange (Citrus aurantium L.). Processes, 7, 363.
  • 16. Ettebong E., Ubulom P., Etuk A., 2019. Antiplasmodial activity of methanol leaf extract of Citrus aurantifolia (Christm) Swingle. J Herbmed Pharmacol, 8, 274-280.
  • 17. Jimenez JC., Pinon A., Dive D., Capron M., Dei-Cas E., Convit J., 2009. Antibody response in children infected with Giardia intestinalis before and after treatment with Secnidazole. Am J Trop Med Hyg, 80, 11-15.
  • 18. Ural K., Aysul N., Voyvoda H., Ulutas B., Aldemir OS., Eren H,. 2014. Single dose of secnidazole treatment against naturally occuring Giardia duodenalis infection in Sakiz lambs. Rev MVZ Cordoba, 19, 4023-4032.
  • 19. Da Silva AS., Castro VS., Tonin AA., Brendler S., Costa MM., Jaques JA., Monteiro SG., 2011. Secnidazole for the treatment of giardiasis in naturally infected cats. Parasitol Int, 60, 429-432.
  • 20. Gultekin M., Ural K., Aysul N., Ayan A., Balikci C., Akyildiz G., 2016. The efficacy of chloroquine treatment of Giardia duodenalis infection in calves. Vlaams Diergeneeskd Tijdschr, 85, 335-341.
  • 21. Karademir U., Ural K., Aysul N., Ayan A., Toplu S., Ortlek O., Balιkci C., Kunyeli A., Erdogan H., 2016. The efficacy of chloroquine treatment against naturally occuring Giardia duodenalis infection in lambs. Rev MVZ Cordoba, 21, 5328-5335.
  • 22. Geurden T., Pohleb H., Sarrea C., Dreesena L., Vercruyssea J., Claerebouta E., 2011. The effcacy of a treatment with fenbendazole against anexperimental Giardia duodenalis infection in lambs. Small Rum Res, 96, 211-215.
  • 23. Ragbetli C., Tanritanir P., Yilmaz H., Yoruk I., Ozdemir H., 2014. Effect on biochemical parameters in naturally infected calves with giardiasis after treatment with albendazole Van Province, Turkey. Indian J Anim Res, 48, 38-44.
  • 24. Geurden T., Claerebout E., Dursin L., Deflandre A., Bernay F., Kaltsatos V., Vercruysse J., 2006. The efficacy of an oral treatment with paromomycin against an experimental infection with Giardia in calves. Vet Parasitol, 135, 241-247.
  • 25. Lalle M., Hanevik K., 2018. Treatment-refractory giardiasis: challenges and solutions. Infect Drug Resist, 11, 1921.
  • 26. Wood dos Santos T., Cristina Pereira Q., Teixeira L., Gambero A., A Villena J., Lima Ribeiro M., 2018. Effects of polyphenols on thermogenesis and mitochondrial biogenesis. Int J Mol Sci, 19, 2757.
  • 27. Paniagua M., Crespo J., Aris A., Devant M., 2019. Citrus aurantium flavonoid extract improves concentrate efficiency, animal behavior, and reduces rumen inflammation of Holstein bulls fed high-concentrate diets. Anim Feed Sci Tech, 258, 114304.
  • 28. Mayaa FAK., Alasadiy YDK., 2015. Effect of cold aqueous plant extract (Citrus aurantium and Solanum melongena) against the Giardia lamblia parasite and Entamoeba histolytica in vitro and measuring the LD50 for extracts in Al-Muthanna province. Res J Pharm Biol Chem Sci, 6, 1241-1252.
  • 29. Abdelqader A., Qarallah B., Al-Ramamneh D., Daş G., 2012. Anthelmintic effects of citrus peels ethanolic extracts against Ascaridia galli. Vet Parasitol, 188, 78-84.

Details

Primary Language Turkish
Subjects Health Care Sciences and Services
Journal Section Araştırma Makaleleri
Authors

Deniz ALIÇ URAL (Primary Author)
Aydın Adnan Menderes Üniversitesi, Veteriner Fakültesi Fakülte Çiftliği
Andorra


Songül ERDOĞAN
Aydın Adnan Menderes Üniversitesi, Veteriner Fakültesi İç Hastalıkları Anabilim Dalı
0000-0002-7833-5519
Türkiye


Canberk BALIKÇI
Harran Üniversitesi, Veteriner Fakültesi, İç Hastalıkları Anabilim Dalı
Türkiye


Adnan AYAN
van Yüzüncü Yıl Üniversitesi Veteriner Fakültesi Genetik Bölümü
Türkiye


Hasan ERDOĞAN
Aydın Adnan Menderes Üniversitesi Veteriner Fakültesi İç Hastalıkları Anabilim Dalı
0000-0001-5141-5108
Türkiye


Kerem URAL
Aydın Adnan Menderes Üniversitesi, Veteriner Fakültesi, İç Hastalıkları Anabilim Dalı,
Türkiye

Publication Date October 31, 2021
Published in Issue Year 2021, Volume 16, Issue 2

Cite

Bibtex @research article { ataunivbd798450, journal = {Atatürk Üniversitesi Veteriner Bilimleri Dergisi}, issn = {1306-6137}, eissn = {2147-9615}, address = {}, publisher = {Ataturk University}, year = {2021}, volume = {16}, number = {2}, pages = {149 - 154}, doi = {10.17094/ataunivbd.798450}, title = {Turunçgil Ekstraktı Anti-giardial Terapötik Destek Sağlayabilir mi?}, key = {cite}, author = {Alıç Ural, Deniz and Erdoğan, Songül and Balıkçı, Canberk and Ayan, Adnan and Erdoğan, Hasan and Ural, Kerem} }
APA Alıç Ural, D. , Erdoğan, S. , Balıkçı, C. , Ayan, A. , Erdoğan, H. & Ural, K. (2021). Turunçgil Ekstraktı Anti-giardial Terapötik Destek Sağlayabilir mi? . Atatürk Üniversitesi Veteriner Bilimleri Dergisi , 16 (2) , 149-154 . DOI: 10.17094/ataunivbd.798450
MLA Alıç Ural, D. , Erdoğan, S. , Balıkçı, C. , Ayan, A. , Erdoğan, H. , Ural, K. "Turunçgil Ekstraktı Anti-giardial Terapötik Destek Sağlayabilir mi?" . Atatürk Üniversitesi Veteriner Bilimleri Dergisi 16 (2021 ): 149-154 <https://dergipark.org.tr/en/pub/ataunivbd/issue/65635/798450>
Chicago Alıç Ural, D. , Erdoğan, S. , Balıkçı, C. , Ayan, A. , Erdoğan, H. , Ural, K. "Turunçgil Ekstraktı Anti-giardial Terapötik Destek Sağlayabilir mi?". Atatürk Üniversitesi Veteriner Bilimleri Dergisi 16 (2021 ): 149-154
RIS TY - JOUR T1 - Turunçgil Ekstraktı Anti-giardial Terapötik Destek Sağlayabilir mi? AU - Deniz Alıç Ural , Songül Erdoğan , Canberk Balıkçı , Adnan Ayan , Hasan Erdoğan , Kerem Ural Y1 - 2021 PY - 2021 N1 - doi: 10.17094/ataunivbd.798450 DO - 10.17094/ataunivbd.798450 T2 - Atatürk Üniversitesi Veteriner Bilimleri Dergisi JF - Journal JO - JOR SP - 149 EP - 154 VL - 16 IS - 2 SN - 1306-6137-2147-9615 M3 - doi: 10.17094/ataunivbd.798450 UR - https://doi.org/10.17094/ataunivbd.798450 Y2 - 2021 ER -
EndNote %0 Atatürk University Journal of Veterinary Sciences Turunçgil Ekstraktı Anti-giardial Terapötik Destek Sağlayabilir mi? %A Deniz Alıç Ural , Songül Erdoğan , Canberk Balıkçı , Adnan Ayan , Hasan Erdoğan , Kerem Ural %T Turunçgil Ekstraktı Anti-giardial Terapötik Destek Sağlayabilir mi? %D 2021 %J Atatürk Üniversitesi Veteriner Bilimleri Dergisi %P 1306-6137-2147-9615 %V 16 %N 2 %R doi: 10.17094/ataunivbd.798450 %U 10.17094/ataunivbd.798450
ISNAD Alıç Ural, Deniz , Erdoğan, Songül , Balıkçı, Canberk , Ayan, Adnan , Erdoğan, Hasan , Ural, Kerem . "Turunçgil Ekstraktı Anti-giardial Terapötik Destek Sağlayabilir mi?". Atatürk Üniversitesi Veteriner Bilimleri Dergisi 16 / 2 (October 2021): 149-154 . https://doi.org/10.17094/ataunivbd.798450
AMA Alıç Ural D. , Erdoğan S. , Balıkçı C. , Ayan A. , Erdoğan H. , Ural K. Turunçgil Ekstraktı Anti-giardial Terapötik Destek Sağlayabilir mi?. Atatürk Üniversitesi Veteriner Bilimleri Dergisi. 2021; 16(2): 149-154.
Vancouver Alıç Ural D. , Erdoğan S. , Balıkçı C. , Ayan A. , Erdoğan H. , Ural K. Turunçgil Ekstraktı Anti-giardial Terapötik Destek Sağlayabilir mi?. Atatürk Üniversitesi Veteriner Bilimleri Dergisi. 2021; 16(2): 149-154.
IEEE D. Alıç Ural , S. Erdoğan , C. Balıkçı , A. Ayan , H. Erdoğan and K. Ural , "Turunçgil Ekstraktı Anti-giardial Terapötik Destek Sağlayabilir mi?", Atatürk Üniversitesi Veteriner Bilimleri Dergisi, vol. 16, no. 2, pp. 149-154, Oct. 2021, doi:10.17094/ataunivbd.798450