Sorghum bicolor L. CAMTA Transkripsiyon Faktörlerinin Genom Çaplı Analizi

Öz

Kalmodulin bağlayıcı transkripsiyon aktivatörü olan CAMTA gen ailesi, bitki familyasında karakterize edilmiş kalmodulin bağlayıcı transkripsiyon faktörleridir. CAMTA gen ailesi hastalıklara karşı direnç, biyotik ve abiyotik stres etmenlerine karşı yanıt gibi çeşitli biyolojik süreçlerde önemli roller üstlenmektedir. Bu çalışmada, sorgum (Sorghum bicolor L.) genomunda 7 CAMTA geni belirlendi ve kök ve sürgün dokularında Sobic-CAMTA genlerinin ifade profilleri analiz edildi. Sobic-CAMTA proteinlerinin moleküler ağılıkları ve uzunlukları sırasıyla 95,22 kDa (Sobic-CAMTA-6) ile 114,86 kDa (Sobic-CAMTA-5) ve 845 (Sobic-CAMTA-6) ila 1030 (Sobic-CAMTA-5) amino asit arasındadır. Sobic-CAMTA genleri arasında tahmini olarak belirlenen ekzonların sayısı en düşük 10 en yüksek 13’tür. İzoelektrik noktaları ise 5,55 (Sobic-CAMTA-5.) ila 8,36 (Sobic-CAMTA-4) arasında değişmektedir. Sobic-CAMTA-2/Sobic-CAMTA-3 tandem duplike genler iken, Sobic-CAMTA-3/Sobic-CAMTA-5 ve Sobic-CAMTA-6/Sobic-CAMTA-7 ise segmental duplike genler olarak tespit edilmiştir. S. bicolor L., Arabidopsis thaliana (L.) Heynh ve Zea mays L. CAMTA proteinleri kullanılarak çizilen filogenetik ağaca göre 3 ana grup (A, B ve C) elde edilmiştir. Sobic-CAMTA genlerinin ifade profilleri, S. bicolor L. bitkisinin farklı dokularına farklı azot kaynağı uygulaması ile belirlenmiştir. Bu çalışmanın sonuçları, sorgum bitkisinde CAMTA transkripsiyon faktörü gen ailesinin moleküler yapısının anlaşılması için önemli bilgiler sağlayacaktır.

Anahtar Kelimeler

• Ankrin
• Kalmodulin
• CG-1 DNA bağlanma domaini
• IQ motifi
• Sorghum bicolor L.

Genome wide analysis of Sorghum bicolor L. CAMTA Transcription Factors

Abstract

Calmodulin binding transcription activators (CAMTAs) are well-characterized in the plants. The CAMTA gene family plays an important role in a variety of biological processes, such as resistance to diseases or response to biotic and abiotic factors. In this study, 7 CAMTA genes were identified in the Sorghum bicolor L. genome and expression profiles of Sobic-CAMTA genes in root and shoot tissues were analyzed. The molecular weight and length of Sobic-CAMTA proteins ranged from 95.22 kDa (Sobic-CAMTA-6) to 114.86 kDa (Sobic-CAMTA-5) and 845 (Sobic-CAMTA-6) to 1030 (Sobic-CAMTA-5) amino acids, respectively. Estimated number of exons determined among Sobic-CAMTA genes was between 10 and 13. The isoelectric points ranged from 5.55 (Sobic-CAMTA-5.) to 8.36 (Sobic-CAMTA-4). Sobic-CAMTA-2/Sobic-CAMTA-3 tandem duplicated genes, while Sobic-CAMTA-3/Sobic-CAMTA-5 and Sobic-CAMTA-6/Sobic-CAMTA-7 were identified as segmentally-duplicated genes. According to the phylogenetic tree, which drawn using CAMTA proteins of S. bicolor L., Arabidopsis thaliana (L.) Heynh and Zea mays L., three main groups (A, B and C) were obtained. The expression profiles of Sobic-CAMTA genes were determined by applying different nitrogen sources to different tissues of S. bicolor L. The results of this study would provide important information to understand the molecular structure of the CAMTA transcription factor gene family in sorghum.

Keywords

• Ankyrin
• Calmodulin
• CG-1 DNA binding domain
• IQ motif
• Sorghum bicolor L.

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