Abstract
ÖZET
Yaprak saydamlaştırma ve fungus boyama metodu özellikle Rhyndwsporium secalis (Oudem) JI Davis'in arpa bitkisi dokularındaki gelişmesini izlemek üzere geliştirilmiştir. Bu metodda enfekteli yapraklar önce laktofenol +metanol+ kloroform karışımında saydamlaştmhp takiben laktofenol-analin mavisi içinde kaynatılarak dokulardaki jimgus boyanmaktadır.
SUMMARY
A Rapid Leaf elearing and Fungus Staining Technique to Study Host-Pathogen Relationships
This leaf clearing and fungus staining tchnique is especially modified to study the development of Rhynchosporiutn secalis (Oudem) J.J. Davis in the tissues of barley plants. in this method infected leaf sections are eleared in a mıxture of
lactophenol + metlıanol + clıoloroform at 50°C for about 1 hour. After c1earing, leaf sections are brought to boiling for about 2-3 minutes in lactophenol-analin blue and then rinsed iri lactophenal. Afterwards Ieaf sections are mounted on mic·
roscope slides in lactophenol for observation.
Keywords
Details
| Primary Language | tr;en |
|---|---|
| Journal Section | ARAŞTIRMALAR |
| Authors | |
| Publication Date | December 30, 2010 |
| Published in Issue | Year 1981 Volume: 12 Issue: 1 |
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