Buğdayda Olgun Embriyo Kültürünü Etkileyen Faktörler

Year 2011, Volume: 42 Issue: 1, 1 - 10, 20.02.2013

Murat Aydın , Sevim Sağsöz Kamil Haliloğlu Metin Tosun

Abstract

Bu çalışma buğdayda olgun embriyo kültürünü etkileyen faktörleri belirlemek amacıyla yapılmıştır. Kallus, embriyogenik
kallus, somatik embriyo oluşumu ve rejenerasyon kapasitesine genotipin, jel yapıcı maddenin, oksin tipleri ve dozlarının etkisi çok
önemli olmuştur. Buğday olgun embriyo kültüründe oksin tipi olarak dikambanın, 2,4-D ve pikloramdan, yine jel yapıcı madde
olarak phytagelin, agardan daha etkiliği olduğu saptanmıştır. En yüksek kallus, embriyogenik kallus ve somatik oluşumu ve de bitki
rejenerasyon kapasitesi phytagel ortamındaki dikambanın 4 mg/l dozunda elde edilmiştir. Rejenerasyon ortamının rejenerasyon
kapasitesine etkisi çok önemli olmuştur. En yüksek rejenerasyon kapasitesi R1 (0.1 mg/l 2,4-D + 0.5 mg/l BAP) içeren ortamda
meydana gelmiştir.

Keywords

Genotip, oksin, jel yapıcı madde, rejenerasyon ortamı, Triticum aestivum

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