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Doğu Anadolu Kırmızısı ve Melezi Sığırların Büyüme Hormonu Geni Genetik Varyasyonunun İncelenmesi

Year 2011, Volume: 42 Issue: 2, 159 - 163, 20.02.2013

Abstract

Bu çalışmada, Doğu Anadolu bölgesinde farklı çiftliklerde yetiştirilen yerli gen kaynağı olarak korunan Doğu Anadolu
Kırmızısı (DAK) yerli sığır ırkı (N=76) ve melezlerinde (51) Büyüme Hormonu (bGH) geni AluI polimorfizmi incelenmiştir.
Çalışmada AluI enzimi uygulanan sığır Büyüme Hormonu genine ait üç genotip (LL, LV ve VV) PCR-RFLP metodu kullanılarak
tanımlanmıştır. L allel frekansı saf DAK’da 0.78, melezlerinde ise 0.79 olarak tespit edilmiştir. Üç genotipin dağılımı saf
DAK’larda; LL %59, LV %37 ve VV %4, ve melezlerde ise LL %63, LV %33 ve VV %4 şeklinde olmuştur. İncelenen
populasyonların CHW dengesinde oldukları gözlendi.

References

  • Bass, J.J., Oldham, J.M., Hodgkinson, S.C., Fowke, P.J., Sauerwein, H., Molan, P., Breier, B.H., Gluckman, P.D. 1991. Influence of nutrition and bovine growth hormone (GH) on hepatic GH binding, insulin-like growth factor-I and growth of lambs. J. Endocrinol, 128(2):181-186.
  • Bauman, D.E., 1992. Bovine somatotropin: review of an emerging animal technology. J. Dairy Sci. 75 (12):3432-3451.
  • Chikuni K., Terada, F., Kageyama, S., Koishikawa, T., Kato, S., Ozutsumi, K., 1991. Identification of DNA sequence variants for amino acid residues 127 of bovine growth hormone using the polymerase chain reaction method. Animal Science and Technology 62, 660-666.
  • Dybus, A., 2002. Associations between Leu/Val polymorphism of growth hormone gene and milk production traits in Black- and-White cattle. Arch. Tierz. Dummerstorf 45 (5): 421-428.
  • Ertuğrul, M., Akman, N., Dellal, G., Goncagül, T., 2000. Hayvan Gen Kaynaklarının Korunması ve Türkiye Hayvan Gen Kaynakları. Türkiye Ziraat Mühendisliği V. Teknik Kongresi. Yayın No:38, Ankara.
  • Ertuğrul, M, Dellal G., Elmacı C., Akın O.A., Pehlivan, E., Soysal, M.İ., Arat, S., 2010. Çiftlik hayvanları genetik kaynaklarının korunması ve sürdürülebilir kullanımı. Türkiye Ziraat Mühendisliği VII. Kongresi. Ankara.
  • Falaki, M., Gengler, N., Sneyers, M., Prandi., A, Massart, S., Formigoni, A., Burny, A., Portetelle, D., Renaville, R., 1996. Relationships of polymorphisms for growth hormone and growth hormone receptor genes with milk production traits for Italian Holstein-Friesian bulls. J. Dairy Sci.79(8):1446- 1453.
  • Fries, R., Egen, A., Womack, J.E. 1993. The bovine genome map. Mamm. Genome. 4:405.
  • Ge, W., Davis, M.E., Hines, H.C., Irvin, K.M., Simmen, C.M. 2003. Association of single nucleotide polymorphisms in the growth hormone and growth hormone receptor genes with serum insulin-like growth factor I concentration and growth traits in Angus cattle. J. Anim. Sci., 81:641–648.
  • Grochowska, R., Lunden, A., Zwierzchowski, L., Snochowski, M., Oprzadek, J., 2001. Association between gene polymorphism of growth hormone and carcass traits in dairy bulls. Anim. Sci. 72: 441-447.
  • Kemenes, P.A., Reginato, L.C.A., Rosa, A.J.M., Parker, I.U., Razook G.A., 1999. β-casein, κ-lactoglobulin and growth hormone allele frequencies and genetic distances in Nelore, Gyr, Guzer, Caracu, Charolais, Canchin and Santa Gertrudis cattle. Genet. Mol. Biol., 22: 539-541.
  • Kovacs, K., Volgyi-Csik, J., Zsolnai, A., Gyorkos I. and Fesus, L., 2006. Associations between the AluI polymorphism of growth hormone gene and production and reproduction traits in a Hungarian Holstein-Friesian bull dam population. Archiv fur Tierzucht, 49, 236–249.
  • Lagziel, A., Lipkin, E., Soller, M., 1996. Association between SSPC haplotypes at the bovine growth hormone gene and milk protein percentage. Genetics, 142: 946-951.
  • Lagziel, A., Lipkin, E., Ezra, E., Soller, M., Weller, J.I. 1999. An MspI polymorphism at the bovine growth hormone (bGH) gene is linked to a locus affecting milk protein percentage. Animal Genetics, 30: 296-299.
  • Lucy, M.C., Hauser, S.D., Eppard, P.J., Krivi, G.G., Clark, J.H., Bauman, D.E., Collier, R.J., 1993. Variants of somatotropin in cattle- gene frequencies in major dairy breeds and associated milk-production. Domestic Animal Endocrinology, 10(4): 325-333. Abst.
  • Mullen, M.P., Lynch, C.O., Waters, S.M., Howard, D.J., O’Boyle, P., Kenny, D.A., Buckley, F., Horan B., Diskin, M.G. 2011.Single nucleotide polymorphisms in the growth hormone and insulin-like growth factor-1 genes are associated with milk production, body condition score and fertility traits in dairy cows. Genetics and Molecular Research 10 (3): 1819-1830.
  • Özdemir, M., 2011. Determination of allele frequencies of growth hormone AluI polymorphism in Brown Swiss, Holstein, native East Anatolian Red and Turkish Grey breeds. International Journal of Dairy Technology. 64(3):350–352.
  • Özdemir, M, Doğru U., 2007. Genetik karakterizasyonda mitokondriyal DNA kullanımı. Atatürk Üniv. Zir. Fak. Derg. 38 (1) 105-111.
  • Özkan, E., Soysal, M.İ., Dinç, H., Sönmez, G., Okyar, M., Togan İ., 2009.Türkiye sığır ırklarında büyüme hormonu AluI ve MspI polimorfizminin PZR-RFLP yönteminin kullanılarak belirlenmesi. 6. Ulusal Zootekni Kongresi, 24-26 Haziran, Erzurum, 93-102.
  • Pal, A., Chakravarty, A.K., Bhattacharya, T.K., Sharma, A. 2005. Polymorphism of growth hormone gene and its association with expected milk production traits in dairy bulls. J. Appl. Anim. Res. 27(1): 29-33.
  • Pawar, R.S., Tajane, K.R., Joshi, C.G., Rank, D.N., Bramkshtri, B.P., 2007. Growth hormone gene polymorphism and its association with lactation yield in dairy cattle. Indian J. Anim Sci. 77(9):884-888.
  • Rocha, J. L., Baker, J. F., Womack, J. E., Sanders, J. O., Taylor, J. F. 1992. Statistical associations between restriction fragment length polymorphisms and quantitative traits in beef cattle. J. Anim.Sci. 70: 3360–3370.
  • Rozen, S., Skaletsky, H.J., 2000. Primer3 on the WWW for general users and for biologist programmers. In Bioinformatics Methods and Protocols: Molecular Biology, pp 365–386. Krawetz S, Misener S, eds. Totowa, NJ, USA: Humana Press, Source code available at http://fokker.wi.mit.edu/primer3/. (27/04/2005).
  • Sabour, M.P., Lin,C.Y., Smith, C.1997. Association of genetic variants of bovine growth hormone with milk production traits in Holstein cattle. Journal of Animal Breeding and Genetics, 114 : 435-442.
  • Sadeghi, M., Moradi Shahr-e-Babak, M., Rahimi G., Javaremi, A. N. 2008. Association Between Gene Polymorphism of Bovine Growth Hormone and Milk Traits in the Iranian Holstein Bulls. Asian Journal of Animal Sciences, 2:1-6.
  • Schlee, P., Graml, R., Rottmann, O., Pirchner, F. 1994a. Influence of growth hormone genotypes on breeding values of Simmental bulls. Journal of Animal Breeding and Genetics, 111: 253-256.
  • Schlee, P., Graml, R., Schallenberger, E., Schams, D., Rottmann, O., Olbrich B.A., Pirchner F. 1994b. Growth hormone and insulin like growth factor I: concentration in bulls of various growth hormone genotypes. Theoretical and Applied Genetics, 88:497-500.
  • Sejrsen, K., Foldager, J., Sorensen, M.T., Akers, R.M., Bauman, D.E. 1986. Effect of exogenous bovine somatotropin on pubertal mammary development in heifers. J. Dairy Sci. 69(6):1528-1535.
  • Taylor, J. F., Coutinho, L. L., Herring, K. L., Gallagher Jr, D. S., Brenneman, R. A., Burney, N., Sanders, J. O., Turner, J. W., Smith, S. B., Miller, R. K., Savell, J. W., Davis, S. K., 1998. Candidate Gene Analysis of Gh1 for effects on growth and carcass composition of cattle. Anim. Genet. 29:194-201.
  • Unanian, M. M., Barreto, C. C., Freitas, A. R., Cordeiro, C. M. T., Josahkian, L. A., 2000. Association of growth hormone gene polymorphisms with weight traits in Nelore breed.. Rev. Bras. Zootec. 29:1380-1386.
  • Vasconcellos, L.P.M.K., Tambasco, D.D., Pereira, A.P., Coutinho, L.L., Regitano, L.C.A. 2003. Genetic characterization of Aberdeen Angus cattle using molecular markers. Genetics and Molecular Biology. 26(2):133-137.
  • Vukasinovic, N., Denise, S.K., Freeman, A.E., 1999. Association of growth hormone loci with milk yield traits in Holstein Bulls. Dairy Sci. 82(4):788-794.
  • Yardibi, H., Hosturk, G.T., Paya, I., Kaygısız, F., Çiftçioğlu, G., Mengi, A., Oztabak, K., 2009. Associations of growth hormone gene polymorphism with milk production traits in South Anatolian and East Anatolian Red cattle. J Anim Vet Adv, 8, 1040-1044.
  • Yao, J., Aggrey, S.E., Zadworny D., Hayes, J.F., Kuhnlein, U. 1996. Sequence variations in the bovine growth hormone gene characterized by single-strand conformation polymorphins (SSCP) analysis and their association with milk production traits in Holsteins. Genetics, 144:1809-1816.
  • Zhou, G., Zhu, Q., Jin, H.G., Guo, S.L., 2006. Genetic variation of growth hormone gene and its relationship with milk production traits in China Holstein cows. Asian-Australasian J. of Anim. Sci. 19(3):315-318.
Year 2011, Volume: 42 Issue: 2, 159 - 163, 20.02.2013

Abstract

References

  • Bass, J.J., Oldham, J.M., Hodgkinson, S.C., Fowke, P.J., Sauerwein, H., Molan, P., Breier, B.H., Gluckman, P.D. 1991. Influence of nutrition and bovine growth hormone (GH) on hepatic GH binding, insulin-like growth factor-I and growth of lambs. J. Endocrinol, 128(2):181-186.
  • Bauman, D.E., 1992. Bovine somatotropin: review of an emerging animal technology. J. Dairy Sci. 75 (12):3432-3451.
  • Chikuni K., Terada, F., Kageyama, S., Koishikawa, T., Kato, S., Ozutsumi, K., 1991. Identification of DNA sequence variants for amino acid residues 127 of bovine growth hormone using the polymerase chain reaction method. Animal Science and Technology 62, 660-666.
  • Dybus, A., 2002. Associations between Leu/Val polymorphism of growth hormone gene and milk production traits in Black- and-White cattle. Arch. Tierz. Dummerstorf 45 (5): 421-428.
  • Ertuğrul, M., Akman, N., Dellal, G., Goncagül, T., 2000. Hayvan Gen Kaynaklarının Korunması ve Türkiye Hayvan Gen Kaynakları. Türkiye Ziraat Mühendisliği V. Teknik Kongresi. Yayın No:38, Ankara.
  • Ertuğrul, M, Dellal G., Elmacı C., Akın O.A., Pehlivan, E., Soysal, M.İ., Arat, S., 2010. Çiftlik hayvanları genetik kaynaklarının korunması ve sürdürülebilir kullanımı. Türkiye Ziraat Mühendisliği VII. Kongresi. Ankara.
  • Falaki, M., Gengler, N., Sneyers, M., Prandi., A, Massart, S., Formigoni, A., Burny, A., Portetelle, D., Renaville, R., 1996. Relationships of polymorphisms for growth hormone and growth hormone receptor genes with milk production traits for Italian Holstein-Friesian bulls. J. Dairy Sci.79(8):1446- 1453.
  • Fries, R., Egen, A., Womack, J.E. 1993. The bovine genome map. Mamm. Genome. 4:405.
  • Ge, W., Davis, M.E., Hines, H.C., Irvin, K.M., Simmen, C.M. 2003. Association of single nucleotide polymorphisms in the growth hormone and growth hormone receptor genes with serum insulin-like growth factor I concentration and growth traits in Angus cattle. J. Anim. Sci., 81:641–648.
  • Grochowska, R., Lunden, A., Zwierzchowski, L., Snochowski, M., Oprzadek, J., 2001. Association between gene polymorphism of growth hormone and carcass traits in dairy bulls. Anim. Sci. 72: 441-447.
  • Kemenes, P.A., Reginato, L.C.A., Rosa, A.J.M., Parker, I.U., Razook G.A., 1999. β-casein, κ-lactoglobulin and growth hormone allele frequencies and genetic distances in Nelore, Gyr, Guzer, Caracu, Charolais, Canchin and Santa Gertrudis cattle. Genet. Mol. Biol., 22: 539-541.
  • Kovacs, K., Volgyi-Csik, J., Zsolnai, A., Gyorkos I. and Fesus, L., 2006. Associations between the AluI polymorphism of growth hormone gene and production and reproduction traits in a Hungarian Holstein-Friesian bull dam population. Archiv fur Tierzucht, 49, 236–249.
  • Lagziel, A., Lipkin, E., Soller, M., 1996. Association between SSPC haplotypes at the bovine growth hormone gene and milk protein percentage. Genetics, 142: 946-951.
  • Lagziel, A., Lipkin, E., Ezra, E., Soller, M., Weller, J.I. 1999. An MspI polymorphism at the bovine growth hormone (bGH) gene is linked to a locus affecting milk protein percentage. Animal Genetics, 30: 296-299.
  • Lucy, M.C., Hauser, S.D., Eppard, P.J., Krivi, G.G., Clark, J.H., Bauman, D.E., Collier, R.J., 1993. Variants of somatotropin in cattle- gene frequencies in major dairy breeds and associated milk-production. Domestic Animal Endocrinology, 10(4): 325-333. Abst.
  • Mullen, M.P., Lynch, C.O., Waters, S.M., Howard, D.J., O’Boyle, P., Kenny, D.A., Buckley, F., Horan B., Diskin, M.G. 2011.Single nucleotide polymorphisms in the growth hormone and insulin-like growth factor-1 genes are associated with milk production, body condition score and fertility traits in dairy cows. Genetics and Molecular Research 10 (3): 1819-1830.
  • Özdemir, M., 2011. Determination of allele frequencies of growth hormone AluI polymorphism in Brown Swiss, Holstein, native East Anatolian Red and Turkish Grey breeds. International Journal of Dairy Technology. 64(3):350–352.
  • Özdemir, M, Doğru U., 2007. Genetik karakterizasyonda mitokondriyal DNA kullanımı. Atatürk Üniv. Zir. Fak. Derg. 38 (1) 105-111.
  • Özkan, E., Soysal, M.İ., Dinç, H., Sönmez, G., Okyar, M., Togan İ., 2009.Türkiye sığır ırklarında büyüme hormonu AluI ve MspI polimorfizminin PZR-RFLP yönteminin kullanılarak belirlenmesi. 6. Ulusal Zootekni Kongresi, 24-26 Haziran, Erzurum, 93-102.
  • Pal, A., Chakravarty, A.K., Bhattacharya, T.K., Sharma, A. 2005. Polymorphism of growth hormone gene and its association with expected milk production traits in dairy bulls. J. Appl. Anim. Res. 27(1): 29-33.
  • Pawar, R.S., Tajane, K.R., Joshi, C.G., Rank, D.N., Bramkshtri, B.P., 2007. Growth hormone gene polymorphism and its association with lactation yield in dairy cattle. Indian J. Anim Sci. 77(9):884-888.
  • Rocha, J. L., Baker, J. F., Womack, J. E., Sanders, J. O., Taylor, J. F. 1992. Statistical associations between restriction fragment length polymorphisms and quantitative traits in beef cattle. J. Anim.Sci. 70: 3360–3370.
  • Rozen, S., Skaletsky, H.J., 2000. Primer3 on the WWW for general users and for biologist programmers. In Bioinformatics Methods and Protocols: Molecular Biology, pp 365–386. Krawetz S, Misener S, eds. Totowa, NJ, USA: Humana Press, Source code available at http://fokker.wi.mit.edu/primer3/. (27/04/2005).
  • Sabour, M.P., Lin,C.Y., Smith, C.1997. Association of genetic variants of bovine growth hormone with milk production traits in Holstein cattle. Journal of Animal Breeding and Genetics, 114 : 435-442.
  • Sadeghi, M., Moradi Shahr-e-Babak, M., Rahimi G., Javaremi, A. N. 2008. Association Between Gene Polymorphism of Bovine Growth Hormone and Milk Traits in the Iranian Holstein Bulls. Asian Journal of Animal Sciences, 2:1-6.
  • Schlee, P., Graml, R., Rottmann, O., Pirchner, F. 1994a. Influence of growth hormone genotypes on breeding values of Simmental bulls. Journal of Animal Breeding and Genetics, 111: 253-256.
  • Schlee, P., Graml, R., Schallenberger, E., Schams, D., Rottmann, O., Olbrich B.A., Pirchner F. 1994b. Growth hormone and insulin like growth factor I: concentration in bulls of various growth hormone genotypes. Theoretical and Applied Genetics, 88:497-500.
  • Sejrsen, K., Foldager, J., Sorensen, M.T., Akers, R.M., Bauman, D.E. 1986. Effect of exogenous bovine somatotropin on pubertal mammary development in heifers. J. Dairy Sci. 69(6):1528-1535.
  • Taylor, J. F., Coutinho, L. L., Herring, K. L., Gallagher Jr, D. S., Brenneman, R. A., Burney, N., Sanders, J. O., Turner, J. W., Smith, S. B., Miller, R. K., Savell, J. W., Davis, S. K., 1998. Candidate Gene Analysis of Gh1 for effects on growth and carcass composition of cattle. Anim. Genet. 29:194-201.
  • Unanian, M. M., Barreto, C. C., Freitas, A. R., Cordeiro, C. M. T., Josahkian, L. A., 2000. Association of growth hormone gene polymorphisms with weight traits in Nelore breed.. Rev. Bras. Zootec. 29:1380-1386.
  • Vasconcellos, L.P.M.K., Tambasco, D.D., Pereira, A.P., Coutinho, L.L., Regitano, L.C.A. 2003. Genetic characterization of Aberdeen Angus cattle using molecular markers. Genetics and Molecular Biology. 26(2):133-137.
  • Vukasinovic, N., Denise, S.K., Freeman, A.E., 1999. Association of growth hormone loci with milk yield traits in Holstein Bulls. Dairy Sci. 82(4):788-794.
  • Yardibi, H., Hosturk, G.T., Paya, I., Kaygısız, F., Çiftçioğlu, G., Mengi, A., Oztabak, K., 2009. Associations of growth hormone gene polymorphism with milk production traits in South Anatolian and East Anatolian Red cattle. J Anim Vet Adv, 8, 1040-1044.
  • Yao, J., Aggrey, S.E., Zadworny D., Hayes, J.F., Kuhnlein, U. 1996. Sequence variations in the bovine growth hormone gene characterized by single-strand conformation polymorphins (SSCP) analysis and their association with milk production traits in Holsteins. Genetics, 144:1809-1816.
  • Zhou, G., Zhu, Q., Jin, H.G., Guo, S.L., 2006. Genetic variation of growth hormone gene and its relationship with milk production traits in China Holstein cows. Asian-Australasian J. of Anim. Sci. 19(3):315-318.
There are 35 citations in total.

Details

Primary Language tr;en
Journal Section ARAŞTIRMALAR
Authors

Memiş Özdemir

Publication Date February 20, 2013
Published in Issue Year 2011 Volume: 42 Issue: 2

Cite

APA Özdemir, M. (2013). Doğu Anadolu Kırmızısı ve Melezi Sığırların Büyüme Hormonu Geni Genetik Varyasyonunun İncelenmesi. Atatürk Üniversitesi Ziraat Fakültesi Dergisi, 42(2), 159-163.
AMA Özdemir M. Doğu Anadolu Kırmızısı ve Melezi Sığırların Büyüme Hormonu Geni Genetik Varyasyonunun İncelenmesi. Atatürk Üniversitesi Ziraat Fakültesi Dergisi. February 2013;42(2):159-163.
Chicago Özdemir, Memiş. “Doğu Anadolu Kırmızısı Ve Melezi Sığırların Büyüme Hormonu Geni Genetik Varyasyonunun İncelenmesi”. Atatürk Üniversitesi Ziraat Fakültesi Dergisi 42, no. 2 (February 2013): 159-63.
EndNote Özdemir M (February 1, 2013) Doğu Anadolu Kırmızısı ve Melezi Sığırların Büyüme Hormonu Geni Genetik Varyasyonunun İncelenmesi. Atatürk Üniversitesi Ziraat Fakültesi Dergisi 42 2 159–163.
IEEE M. Özdemir, “Doğu Anadolu Kırmızısı ve Melezi Sığırların Büyüme Hormonu Geni Genetik Varyasyonunun İncelenmesi”, Atatürk Üniversitesi Ziraat Fakültesi Dergisi, vol. 42, no. 2, pp. 159–163, 2013.
ISNAD Özdemir, Memiş. “Doğu Anadolu Kırmızısı Ve Melezi Sığırların Büyüme Hormonu Geni Genetik Varyasyonunun İncelenmesi”. Atatürk Üniversitesi Ziraat Fakültesi Dergisi 42/2 (February 2013), 159-163.
JAMA Özdemir M. Doğu Anadolu Kırmızısı ve Melezi Sığırların Büyüme Hormonu Geni Genetik Varyasyonunun İncelenmesi. Atatürk Üniversitesi Ziraat Fakültesi Dergisi. 2013;42:159–163.
MLA Özdemir, Memiş. “Doğu Anadolu Kırmızısı Ve Melezi Sığırların Büyüme Hormonu Geni Genetik Varyasyonunun İncelenmesi”. Atatürk Üniversitesi Ziraat Fakültesi Dergisi, vol. 42, no. 2, 2013, pp. 159-63.
Vancouver Özdemir M. Doğu Anadolu Kırmızısı ve Melezi Sığırların Büyüme Hormonu Geni Genetik Varyasyonunun İncelenmesi. Atatürk Üniversitesi Ziraat Fakültesi Dergisi. 2013;42(2):159-63.

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