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Year 2013, Volume: 44 Issue: 1, 21 - 25, 27.05.2014

Kamil Halıloglu Kamil Haliloğlu , İsmail Bezirganoğlu İsmail Bezırganoğlu Murat Aydın Metin Tosun Esin Şahin

Abstract

Selecting the explant genotypes is crucial step in in-vitro culture and Agrobacterium-mediated transformationsystem due to its host range specificity. Poor tissue culture performance is restricting the number of wheat genotypes that can bestably transformed. Response of fourteen cultivars of winter wheat (Triticum aestivum L.) to immature embryo culture, callusproduction and plant regeneration was evaluated. A significant (p < 0.05) genotypic difference in callus induction frequency wasobserved ranging between 45% (cv. Sürak) and 99.54% (cv. Bobwhite) of the cultured explants. Plant regeneration through somaticembryogenesis was observed from calli of all 15 cultivars after a 4-week callus induction period. Genotypes with the highestnumber of plantles per cultured embryo were Sivas-111/33 (8.66), Kıraç-66 (7.36), Haymana 79 (6.77) and Conkesme (6.56). Fourwinter wheat genotypes, ‘Sivas-111/33, Kirac-66, Haymana 79, and Conkesme’, have been selected for initial Agrobacteriummediated transformation experiments due to their high regeneration potentials.

Keywords

Winter wheat callus formation plant regeneration immature embryos

References

  • Aydin, M., M. Tosun, K. Haliloglu, 2011. Plant regeneration in wheat mature embryo culture. African Journal of Biotechnology. 10(70): 15749-15755.
  • Ahloowalia, B.S., 1982. Plat regeneration from callus culture in wheat. Crop Sci. 22: 405-410
  • Barro, F., A. Martin, P.A. Lazzeri, P. Barceló, 1999. Medium optimization for efficient somatic embryogenesis and plant regeneration from immature inflorescences and immature scutella of elite cultivars of wheat, barley and tritordeum. Euphytica 108:: 161–167.
  • Boulton, M.I., H. Steinkellner, J. Donson, P.G. Markham, D.I. King, K.W. Davis, 1989.: Mutational analysis of the virionsense genes of maize streak virus. J. Gen. Virol.: 70: 23092323.
  • Carman, J.G., N.E. Jefferson, W.F. Campbell, 1988. Induction of Embryogenic Triticum aestivem L. calli. I. Quantification of genotype and culture medium effects, Plant Cell Tiss. Organ Cult. 10: 101-107.
  • Cheng, M., E. Joyce, S. Pang, H. Zhou, C.M. Hironaka, D.R. Duncan, T.W. Conner, Y. Wan, 1997. Genetic Transformation of Wheat Mediated by Agrobacterium tumefacience. Plant Physiol. 115: 971-980.
  • Deambrogio, E., P.J. Dale, 1980. Effect of 2,4-D on the frequency of regenerated plants in barley and on genetic variability between them. Cereal Res. Commmun. 8: 417–423.
  • Fennell, S., N. Bohorova, M. Ginkel, J. Crossa, D. Hoisington, 1996. Plant regeneration from immature embryos of 48 elite CIMMYT bread wheats, Theor. Appl. Genet. 92: 163–169.
  • Galiba, G., G. Kovacs, J. Sutka, 1986. Substitution analysis of plant regeneration from callus culture in wheat. Plant Breed. 97: 261-263.
  • Haliloglu, K., P.S. Baenziger, 2003. Agrobacterium tumefaciens mediated wheat transformation. Cereal Research Communications 31: 9-16.
  • Haliloglu, K., A. Ozturk, M. Tosun, and S. Bulut, 2005. Relationship between tissue culture and agronomic traits of winter wheat. Cereal Res. Commun. 33,469-476.
  • He, D.G., T.M. Yang, J. Bertram, K.J. Scott, 1990. The histological development of the regenerative tissue derived from cultured immature embryos of wheat. Plant Sci. 68: 103-107.
  • Kaleikau, E.K., R.G. Sears, B.S. Gill, 1989. Control of tissue culture response in wheat (Triticum aestivum L.) Theor. Appl. Genet. 78: 783-787.
  • Machii, H., H. Mizuno, T. Hirabayashi, H. Li, T. Hagio, 1998. Screening wheat genotypes for high callus induction and regeneration capability from anther and immature embryo cultures. Plant Cell Tissue Organ Cult. 53: 67–74.
  • Maddock, S.E., V.A. Lancaster, R. Risiott, J. Franklin, 1987. Plant regeneration from cultured immature embryos and inflorences of 25 cultivars of wheat. Exp. Bot. 34: 915-920.
  • Maheshwary, N., K. Rajyalakshmi, K. Baweja, S.K. Dhir, C.N. Chowdhry, S.C. Maheshwary, 1995. In vitro Culture of Wheat and Genetic Transformation- Retropect and Prospect. Critical Reviews in Plant Sciences 14: 149-178.
  • Mathias, R., E. Atkinson, 1988. In vitro expression of genes affecting whole plant phenotype. The effect of Rht/Gai alleles on the callus culture response of wheat (Triticum aestivum L. em. Thell). Theor. Appl. Genet. 75: 474–479.
  • Mathias, R.J., K. Fukui, 1986. The effect of specific chromosome and cytoplasm substitutions on the tissue culture response of wheat (Triticum aestivum) callus. Theor. Appl. Genet., 71: 797–800.
  • Mathias, R.J., E.S. Simpson, 1986. The interaction of genotype and culture medium on the tissue culture responses of wheat (Triticum aestivum L.) callus. Plant Cell Tiss. Org. Cult. 7: 31-37
  • Mooney P.A., P.B. Goodwin, E.S. Dennis, D.J. Liewellyn, 1991. Agrobacterium tumefacience-gene transfer into wheat tissues. Plant Cell Tiss. Org. Cult. 25: .209-218.
  • Murashige. T., F. Skoog, 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant., 15: 473-497.
  • Murata, M., 1989. Effect of auxin and cytokinin on induction of sister chromatid exchanges in cultured cells of wheat (Triticum aestivum L.). Theor. Appl. Genet. 78: 521–524.
  • Ozias-Akins, P., I.K. Vasil, 1982. Plant regeneration from cultred immature embryos and inflorescences of Triticum aestivum L. (wheat) evidence for somatic embryogenesis. Protoplasma 110: 95-105.
  • Redway, F.A., V. Vasil, I.K. Vasil, 1990. Characterization and regeneration of wheat embyogenic cell suspension cultures, Plant Cell Reports 8: 714-718.
  • SAS Institute Inc., 1996. SAS/STAT Software. Changes and Enhancements Through Release 6.11. SAS Institute, Cary, NC.
  • Sears, R.G., E.L. Deckard, 1982. Tissue culture variability in wheat. callus induction and plant regeneration. Crop Sci. 22: 546-550.
  • Shimada T., 1978. Plant regeneration from the callus induced from wheat embryos. Jpn. J. Genet., 53: 379–385.
  • Takumi S., T. Shimada, 1997. Variation in transformation frequencies among six common wheat cultivars through particle bombardment of scutellar tissues. Genes Genet. Syst. 72: 63–69.
  • Viertel K., A. Schmid, M. Iser, D. Hess, 1998. Regeneration of German spring wheat varities from embryogenic scutellar callus. J. Plant Physiol. 152: 167–172.
  • Waller R.A., D.B. Duncan, 1969. A Bayes rule for the symmetric multiple comparison problems. J. Am. Stat. Assoc. 64:14841503.
  • Weeks J.T., O.D. Anderson, A.E. Blechl, 1993. Rapid production of multiple independent lines of fertile transgenic wheat (Triticum aestivum), Plant Physiology. 102:1077-1084.
  • Zhou H., J.W. Arrowsmith, M.E. Fromm, C.M. Hironaka, M.L. Taylor, D. Rodriguez, M.E. Pajeau, S.M. Brown, C.G. Santino, J.E. Fry, 1995. Glyphosate-tolerant CP4 and GOX genes as a selectable marker in wheat transformation. Plant Cell Rep., 15:159-163.

Türkiye’de Yetiştirilen Bazi Kişlik Buğday Çeştilerinin Olgunlaşmamiş Embriyo Kültürüne Verdiklerin Cevaplarin Belirlenmesi

Year 2013, Volume: 44 Issue: 1, 21 - 25, 27.05.2014

Kamil Halıloglu Kamil Haliloğlu , İsmail Bezirganoğlu İsmail Bezırganoğlu Murat Aydın Metin Tosun Esin Şahin

Abstract

Agrobacterium kaynaklı gen aktarımı ve in vitro kültür çalışmaları için kullanılacak eksplant genotipin seçimi önemli bir
adımdır. Düşük doku kültürü performansı, stabil gen aktarılabilecek buğday genotiplerin sayısını sınırlamaktadır. 14 kışlık buğday
çeşitinin olgunlaşmamış embriyo kültürü, kalus üretimi ve bitki rejenerasyonuna tepkisi değerlendirilmiştir. Kültüre
alınaneksplantların kallus oluşum frekansı %45 (cv. Sürak) ve % 99.54 (cv. Bobwhite) arasındaki oranlarda gözlenmiş ve genotipik
farklılık önemli olmuştur. Somatik embriyogenesis aracılığıyla bitki rejnerasyonu 4 haftalık kallus oluşum periyodundan sonra tüm
çeşitlerin kalluslarında görülmüştür. Kültüre alınan embriyo başına en yüksek bitkicik sayısı Sivas-111/33 (8.66), Kıraç-66 (7.36),
Haymana 79 (6.77) and Conkesme 79 (6.56) çeşitlerinde meydana gelmiştir. Dört kışlık buğday genotipi (Sivas-111/33, Kirac-66,
Haymana 79, and Conkesme) yüksek rejenerasyon potansiyeline sahip olmaları nedeniyle Agrobacterium aracılığıyla gen aktarım
çalışmalarında kullanmak için seçilmiştir.

References

  • Aydin, M., M. Tosun, K. Haliloglu, 2011. Plant regeneration in wheat mature embryo culture. African Journal of Biotechnology. 10(70): 15749-15755.
  • Ahloowalia, B.S., 1982. Plat regeneration from callus culture in wheat. Crop Sci. 22: 405-410
  • Barro, F., A. Martin, P.A. Lazzeri, P. Barceló, 1999. Medium optimization for efficient somatic embryogenesis and plant regeneration from immature inflorescences and immature scutella of elite cultivars of wheat, barley and tritordeum. Euphytica 108:: 161–167.
  • Boulton, M.I., H. Steinkellner, J. Donson, P.G. Markham, D.I. King, K.W. Davis, 1989.: Mutational analysis of the virionsense genes of maize streak virus. J. Gen. Virol.: 70: 23092323.
  • Carman, J.G., N.E. Jefferson, W.F. Campbell, 1988. Induction of Embryogenic Triticum aestivem L. calli. I. Quantification of genotype and culture medium effects, Plant Cell Tiss. Organ Cult. 10: 101-107.
  • Cheng, M., E. Joyce, S. Pang, H. Zhou, C.M. Hironaka, D.R. Duncan, T.W. Conner, Y. Wan, 1997. Genetic Transformation of Wheat Mediated by Agrobacterium tumefacience. Plant Physiol. 115: 971-980.
  • Deambrogio, E., P.J. Dale, 1980. Effect of 2,4-D on the frequency of regenerated plants in barley and on genetic variability between them. Cereal Res. Commmun. 8: 417–423.
  • Fennell, S., N. Bohorova, M. Ginkel, J. Crossa, D. Hoisington, 1996. Plant regeneration from immature embryos of 48 elite CIMMYT bread wheats, Theor. Appl. Genet. 92: 163–169.
  • Galiba, G., G. Kovacs, J. Sutka, 1986. Substitution analysis of plant regeneration from callus culture in wheat. Plant Breed. 97: 261-263.
  • Haliloglu, K., P.S. Baenziger, 2003. Agrobacterium tumefaciens mediated wheat transformation. Cereal Research Communications 31: 9-16.
  • Haliloglu, K., A. Ozturk, M. Tosun, and S. Bulut, 2005. Relationship between tissue culture and agronomic traits of winter wheat. Cereal Res. Commun. 33,469-476.
  • He, D.G., T.M. Yang, J. Bertram, K.J. Scott, 1990. The histological development of the regenerative tissue derived from cultured immature embryos of wheat. Plant Sci. 68: 103-107.
  • Kaleikau, E.K., R.G. Sears, B.S. Gill, 1989. Control of tissue culture response in wheat (Triticum aestivum L.) Theor. Appl. Genet. 78: 783-787.
  • Machii, H., H. Mizuno, T. Hirabayashi, H. Li, T. Hagio, 1998. Screening wheat genotypes for high callus induction and regeneration capability from anther and immature embryo cultures. Plant Cell Tissue Organ Cult. 53: 67–74.
  • Maddock, S.E., V.A. Lancaster, R. Risiott, J. Franklin, 1987. Plant regeneration from cultured immature embryos and inflorences of 25 cultivars of wheat. Exp. Bot. 34: 915-920.
  • Maheshwary, N., K. Rajyalakshmi, K. Baweja, S.K. Dhir, C.N. Chowdhry, S.C. Maheshwary, 1995. In vitro Culture of Wheat and Genetic Transformation- Retropect and Prospect. Critical Reviews in Plant Sciences 14: 149-178.
  • Mathias, R., E. Atkinson, 1988. In vitro expression of genes affecting whole plant phenotype. The effect of Rht/Gai alleles on the callus culture response of wheat (Triticum aestivum L. em. Thell). Theor. Appl. Genet. 75: 474–479.
  • Mathias, R.J., K. Fukui, 1986. The effect of specific chromosome and cytoplasm substitutions on the tissue culture response of wheat (Triticum aestivum) callus. Theor. Appl. Genet., 71: 797–800.
  • Mathias, R.J., E.S. Simpson, 1986. The interaction of genotype and culture medium on the tissue culture responses of wheat (Triticum aestivum L.) callus. Plant Cell Tiss. Org. Cult. 7: 31-37
  • Mooney P.A., P.B. Goodwin, E.S. Dennis, D.J. Liewellyn, 1991. Agrobacterium tumefacience-gene transfer into wheat tissues. Plant Cell Tiss. Org. Cult. 25: .209-218.
  • Murashige. T., F. Skoog, 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant., 15: 473-497.
  • Murata, M., 1989. Effect of auxin and cytokinin on induction of sister chromatid exchanges in cultured cells of wheat (Triticum aestivum L.). Theor. Appl. Genet. 78: 521–524.
  • Ozias-Akins, P., I.K. Vasil, 1982. Plant regeneration from cultred immature embryos and inflorescences of Triticum aestivum L. (wheat) evidence for somatic embryogenesis. Protoplasma 110: 95-105.
  • Redway, F.A., V. Vasil, I.K. Vasil, 1990. Characterization and regeneration of wheat embyogenic cell suspension cultures, Plant Cell Reports 8: 714-718.
  • SAS Institute Inc., 1996. SAS/STAT Software. Changes and Enhancements Through Release 6.11. SAS Institute, Cary, NC.
  • Sears, R.G., E.L. Deckard, 1982. Tissue culture variability in wheat. callus induction and plant regeneration. Crop Sci. 22: 546-550.
  • Shimada T., 1978. Plant regeneration from the callus induced from wheat embryos. Jpn. J. Genet., 53: 379–385.
  • Takumi S., T. Shimada, 1997. Variation in transformation frequencies among six common wheat cultivars through particle bombardment of scutellar tissues. Genes Genet. Syst. 72: 63–69.
  • Viertel K., A. Schmid, M. Iser, D. Hess, 1998. Regeneration of German spring wheat varities from embryogenic scutellar callus. J. Plant Physiol. 152: 167–172.
  • Waller R.A., D.B. Duncan, 1969. A Bayes rule for the symmetric multiple comparison problems. J. Am. Stat. Assoc. 64:14841503.
  • Weeks J.T., O.D. Anderson, A.E. Blechl, 1993. Rapid production of multiple independent lines of fertile transgenic wheat (Triticum aestivum), Plant Physiology. 102:1077-1084.
  • Zhou H., J.W. Arrowsmith, M.E. Fromm, C.M. Hironaka, M.L. Taylor, D. Rodriguez, M.E. Pajeau, S.M. Brown, C.G. Santino, J.E. Fry, 1995. Glyphosate-tolerant CP4 and GOX genes as a selectable marker in wheat transformation. Plant Cell Rep., 15:159-163.
There are 32 citations in total.

Details

Primary Language tr;en
Journal Section ARAŞTIRMALAR
Authors

Kamil Halıloglu This is me

Kamil Haliloğlu

İsmail Bezirganoğlu This is me

İsmail Bezırganoğlu This is me

Murat Aydın This is me

Metin Tosun This is me

Esin Şahin This is me

Publication Date May 27, 2014
Published in Issue Year 2013 Volume: 44 Issue: 1

Cite

APA Halıloglu, K., Haliloğlu, K., Bezirganoğlu, İ., Bezırganoğlu, İ., et al. (2014). Türkiye’de Yetiştirilen Bazi Kişlik Buğday Çeştilerinin Olgunlaşmamiş Embriyo Kültürüne Verdiklerin Cevaplarin Belirlenmesi. Atatürk Üniversitesi Ziraat Fakültesi Dergisi, 44(1), 21-25.
AMA Halıloglu K, Haliloğlu K, Bezirganoğlu İ, Bezırganoğlu İ, Aydın M, Tosun M, Şahin E. Türkiye’de Yetiştirilen Bazi Kişlik Buğday Çeştilerinin Olgunlaşmamiş Embriyo Kültürüne Verdiklerin Cevaplarin Belirlenmesi. Atatürk Üniversitesi Ziraat Fakültesi Dergisi. May 2014;44(1):21-25.
Chicago Halıloglu, Kamil, Kamil Haliloğlu, İsmail Bezirganoğlu, İsmail Bezırganoğlu, Murat Aydın, Metin Tosun, and Esin Şahin. “Türkiye’de Yetiştirilen Bazi Kişlik Buğday Çeştilerinin Olgunlaşmamiş Embriyo Kültürüne Verdiklerin Cevaplarin Belirlenmesi”. Atatürk Üniversitesi Ziraat Fakültesi Dergisi 44, no. 1 (May 2014): 21-25.
EndNote Halıloglu K, Haliloğlu K, Bezirganoğlu İ, Bezırganoğlu İ, Aydın M, Tosun M, Şahin E (May 1, 2014) Türkiye’de Yetiştirilen Bazi Kişlik Buğday Çeştilerinin Olgunlaşmamiş Embriyo Kültürüne Verdiklerin Cevaplarin Belirlenmesi. Atatürk Üniversitesi Ziraat Fakültesi Dergisi 44 1 21–25.
IEEE K. Halıloglu, K. Haliloğlu, İ. Bezirganoğlu, İ. Bezırganoğlu, M. Aydın, M. Tosun, and E. Şahin, “Türkiye’de Yetiştirilen Bazi Kişlik Buğday Çeştilerinin Olgunlaşmamiş Embriyo Kültürüne Verdiklerin Cevaplarin Belirlenmesi”, Atatürk Üniversitesi Ziraat Fakültesi Dergisi, vol. 44, no. 1, pp. 21–25, 2014.
ISNAD Halıloglu, Kamil et al. “Türkiye’de Yetiştirilen Bazi Kişlik Buğday Çeştilerinin Olgunlaşmamiş Embriyo Kültürüne Verdiklerin Cevaplarin Belirlenmesi”. Atatürk Üniversitesi Ziraat Fakültesi Dergisi 44/1 (May 2014), 21-25.
JAMA Halıloglu K, Haliloğlu K, Bezirganoğlu İ, Bezırganoğlu İ, Aydın M, Tosun M, Şahin E. Türkiye’de Yetiştirilen Bazi Kişlik Buğday Çeştilerinin Olgunlaşmamiş Embriyo Kültürüne Verdiklerin Cevaplarin Belirlenmesi. Atatürk Üniversitesi Ziraat Fakültesi Dergisi. 2014;44:21–25.
MLA Halıloglu, Kamil et al. “Türkiye’de Yetiştirilen Bazi Kişlik Buğday Çeştilerinin Olgunlaşmamiş Embriyo Kültürüne Verdiklerin Cevaplarin Belirlenmesi”. Atatürk Üniversitesi Ziraat Fakültesi Dergisi, vol. 44, no. 1, 2014, pp. 21-25.
Vancouver Halıloglu K, Haliloğlu K, Bezirganoğlu İ, Bezırganoğlu İ, Aydın M, Tosun M, Şahin E. Türkiye’de Yetiştirilen Bazi Kişlik Buğday Çeştilerinin Olgunlaşmamiş Embriyo Kültürüne Verdiklerin Cevaplarin Belirlenmesi. Atatürk Üniversitesi Ziraat Fakültesi Dergisi. 2014;44(1):21-5.

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