Portakalda Antraknoz Hastalığı Etmeni Colletotrichum gloeosporioides’in Biyolojik Mücadele İmkanlarının Araştırılması

Year 2019, Volume: 50 Issue: 3, 282 - 291, 22.09.2019

Nasibe Tekiner , Elif Tozlu , Recep Kotan

https://doi.org/10.17097/ataunizfd.544964

Cited By: 2

Abstract

Fungisitlerin
insan ve çevre sağlığını olumsuz etkilemesi, hastalık etmenlerinin fungisitlere
karşı direnç oluşturması ve tarımsal ürünlerdeki kalıntı sorunu kimyasal
mücadeleye alternatif yöntemlerden olan biyolojik mücadelenin önemini bir kat
daha artırmıştır. Sebze ve meyvelerde yaygın olarak görülen antraknoz hastalığı
etmeninin (Colletotrichum gloesporoides)
in vitro koşullarda mikrobiyal
antagonistlerle mücadele edilebilirliğinin araştırıldığı bu çalışmada toplam 9
adet biyoajan bakteri izolatı [Bacillus
megaterium (TV 3D), Bacillus subtilis
(TV 6F, TV 17C, CP 1), Bacillus cereus
(TV 85D), Paenibacillus polymxa (TV
12E), Pantoea agglomerans (RK 79, RK
92), Pseudomonas fluorescens (MF 3)]
ve 3 adet biyoajan fungus izolatı [Trichoderma
harzianum (ET 4, ET 14, NT 1)] kullanılmıştır. Biyoajan fungusların
patojene karşı etkililiği petri kaplarına karşılıklı konularak, bakterilerin
ise merkeze patojen konulup etrafına bakteri çizilerek yapılmıştır. Uygulama sonucunda
tüm mikrobiyal antagonistlerin in vitro
şartlarda patojen fungusun gelişimini engellediği ve P. fluorescens’in MF 3 izolatının (%69,05) ve T. harzianum’un ET 4 izolatının (%82,50) en etkili izolatlar olduğu
belirlenmiştir. 

Keywords

Portakal, Colletotrichum gloesporoides, Pseudomonas fluorescens, Trichoderma harzianum, Biyolojik mücadele

Investigation of Biological Control Possibility of Anthracnose Disease Agent, Colletotrichum gloeosporioides, on Orange

Abstract

The negative effect of fungicides on human and
environmental health, resistance to fungicides and the problem of residue in
agricultural products are getting increase the importance of biological control
one of the alternative methods for chemical control. Nine bioagent bacterial
isolates [Bacillus megaterium (TV
3D), Bacillus subtilis (TV 6F, TV
17C, CP 1), Bacillus cereus (TV 85D),
Paenibacillus polymxa (TV 12E), Pantoea agglomerans (RK 79, RK 92), Pseudomonas fluorescens (MF 3)] and three
bioagent fungi isolates [Trichoderma
harzianum (ET 4, ET 14, NT 1)] were used in this study the investigation of
biological control of anthracnose disease (Colletotrichum
gloesporoides) common in vegetables and fruits in in vitro. As a result of the application, it was determined that
all microbial antagonists prevented the development of pathogen fungus in vitro and P. fluorescens MF 3 isolate (69.05%) and T. harzianum ET 4 isolates (82.50%) were the most effective
isolates. 

Keywords

Orange, Colletotrichum gloesporoides, Pseudomonas fluorescens, Trichoderma harzianum, Biological control

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