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Chemical Components, Antimicrobial and Antioxidant Activities of Nest Materials Vespa crabro germana Christ, 1791 (Hymenoptera: Vespidae)

Year 2020, Volume: 51 Issue: 1, 44 - 52, 25.01.2020
https://doi.org/10.17097/ataunizfd.593016

Abstract

Social wasps build their nests in nature by using
various organic and inorganic materials. In this study, the total phenolic
content, antioxidant potentials based on ferric reducing antioxidant power
(FRAP) and DPPH radical scavenging activity, biochemical composition and
antimicrobial activity of the ethanol extract of
Vespa crabro germana Christ,
1791 nest were investigated. Antibacterial and antifungal activity was
determined by disc diffusion method against 8 bacteria and 2 fungi species. The
ethanol extract of the investigated nest sample showed maximum antimicrobial
activities against
Proteus vulgaris, Bacillus
subtillis, Micrococcus luteus
and Candida
albicans
. On the other hand, the minimum activities obtained against Pseudomonas aeruginosa, Yersinia
enterocolitica, Aspergillus niger
and Klebsiella
pneumoniae
. Total phenolic content (TPC) of sample extract was analyzed
using Folin–Ciocalteu assay and calculated as 0.56 mg gallic acid equivalent/g
sample. FRAP value and DPPH radical scavenging activity of the extract value were
determined as 1.94 mg trolox equivalent/g sample and 0.88 mg trolox equivalent/g
sample, respectively. GC-MS analysis of the sample was according to solid phase
microextraction (SPME) technique and 44 compounds were identified. The presence
of these compounds with bioactive potential, add value to investigated sample
extract with a pharmaceutical meaning as antifungal, antibacterial, antiviral
agents and so on. In other words, the antioxidative and antimicrobial potantial
of the nest sample could be attributed to presence of these compounds. For this
reason, the materials used in the structure of the nests used for breeding by
the social wasp are important for the biological activities.

References

  • Akyüz, E., Şahin, H., Islamoglu, F., Kolayli, S., Sandra, P., 2014. Evaluation of phenolic compounds in Tilia rubra subsp. caucasica by HPLC-UV and HPLC-UV-MS/MS. International Journal of Food Properties., 17(2): 331-343.
  • Anderson, K.E., Sheehan, T.H., Eckholm, B.J., Mott, B.M., DeGrandi-Hoffman, G. 2011. An emerging paradigm of colony health: microbial balance of the honey bee and hive (Apis mellifera). Insectes Sociaux., 58 (4): 431.
  • Bagriacik, N., 2011. Determination of some structural features of the nest paper of Vespa orientalis Linneaus, 1771 and Vespa crabro Linneaus, 1758 (Hymenoptera: Vespinae) in Turkey. Archives of Biological Sciences., 63(2): 449-455.
  • Banks, B.E., Shipolini, R.A., 1986. Chemistry and pharmacology of honey-bee venom. Venoms of the Hymenoptera: Biochemical, pharmacological and behavioural aspects., 330-416.
  • Benzie, I.F., Strain, J.J., 1996. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Analytical biochemistry., 239(1): 70-76.
  • Blum, M.S., Walker, J.R., Callahan, P.S., & Novak, A.F., 1958. Chemical, insecticidal, and antibiotic properties of fire ant venom. Science., 128(3319): 306-307.
  • Christe, P., Oppliger, A., Bancalà, F., Castella, G., Chapuisat, M., 2003. Evidence for collective medication in ants. Ecology Letters., 6(1): 19-22.
  • Ertürk, Ö., Kati, H., Yayli, N., Demirbağ, Z., 2003. Antimicrobial activity of Viscum album L. subsp. abietis (Wiesb). Turkish Journal of Biology., 27(4): 255-258.
  • Ertürk, Ö., Karakaş, F.P., Pehlivan, D., Nas, N., 2009. The antibacterial and antifungal effects of Rhododendron derived mad honey and extracts of four Rhododendron species. Turkish Journal of Biology., 33(2): 151-158.
  • Jeanne, R.L., 1977. Ultimate factors in social wasp nesting behavior. Proceedings of the International Congress of the International Union for the Study of Social Insects., 164-168.
  • Jeanne, R.L., 1986. The organization of work in Polybia occidentalis: costs and benefits of specialization in a social wasp. Behavioral Ecology and Sociobiology., 19(5): 333-341.
  • Jeanne, R.L., 1996. The evolution of exocrine gland function in wasps. Natural History and Evolution of Paper Wasps., 145-160.
  • Langenheim, J.H., 2003. Plant resins: chemistry, evolution, ecology, and ethnobotany (No. 620.1924 L275p). Oregon, US: Timber Press.
  • Lokvam, J., Braddock, J.F., 1999. Anti-bacterial function in the sexually dimorphic pollinator rewards of Clusia grandiflora (Clusiaceae). Oecologia., 119(4): 534-540.
  • Maschwitz, U., Dorow, W.H.O., Botz, T., 1990. Chemical composition of the nest walls, and nesting behaviour, of Ropalidia (Icarielia) opifex van der Vecht, 1962 (Hymenoptera: Vespidae), a Southeast Asian social wasp with translucent nests. Journal of Natural History., 24(5): 1311-1319.
  • McGovern, J.N., Jeanne, R.L., Effland, M.J., 1988. The nature of wasp nest paper. Tappi Journal., 71: 133-139.
  • Monteiro, M.C., Romão, P.R., Soare, A.M., 2009. Pharmacological perspectives of wasp venom. Protein and peptide letters., 16(8): 944-952.
  • Ronald, M.A., 1990. Microbiologia. Compania editorial continental SA de CV, Mexico, DF, 505.
  • Rosengaus, R.B., Guldin, M.R., Traniello, J.F., 1998. Inhibitory effect of termite fecal pellets on fungal spore germination. Journal of Chemical Ecology., 24(10): 1697-1706.
  • Rukaiyat, M., Garba, S., Labaran, S., 2015. Antimicrobial activities of hexacosane isolated from Sanseveria liberica (Gerome and Labroy) plant. Adv Med Plant Res., 3(3): 120-125.
  • Schmid-Hempel, P., 1998. Parasites in social insects. Princeton University Press.
  • Schremmer, F., Marz, L., Simonsberger, P., 1985. Chitin im Speichel der Papierwespen (soziale Faltenwespen, Vespidae): Biologie, Chemismus, Feinstruktur. Mikroskopie., 42(1-2): 52-56.
  • Silva, T.M.S., Camara, C.A., da Silva Lins, A.C., Barbosa-Filho, J.M., da Silva, E.M.S., Freitas, B.M., dos Santos, F.D.A.R., 2006. Chemical composition and free radical scavenging activity of pollen loads from stingless bee Melipona subnitida Ducke. Journal of food composition and analysis., 19(6-7): 507-511.
  • Singleton, V.L., Rossi, J.A., 1965. Colorimetry of total phenolics with phosphomolybdic phosphotungstic acid reagents. Am J Enol Viticult., 16: 144-158.
  • Smania, E.F.A., Monoache, F.D., Yunes, R.A., Pauler, R., Junior, A.S., 2007. Antimicrobial activity of methyl australate from Ganoderma australe. Brazilian Journal of Pharmacognosy., 17(1): 14-16.
  • Spradbery, J.P., 1973. Wasps. An account of the biology and natural history of social and solitary wasps, with particular reference to those of the British Isles.
  • Starr, C.K., 1991. The nest as the locus of social life. The social biology of wasps., 520-539.
  • Subbaiyan, B., Samydurai, P., KarthikPrabu, M., Thangapandian, V., 2014. Gas chromatography and mass spectrum analysis of Catharanthus pusillus (Murray) g. Don (Apocyanaceae). International Research Journal of Pharmaceutical and Applied Sciences (IRJPAS)., 4(2): 4.
  • Tamil Muthu, P., Selvaraj, D., 2015. Analysis of Bioactive Constituents from the Flesh of Turbo brunneus (Roding, 1798) by GCMS. International Journal of Fisheries and Aquatic Studies., 3(1): 257-259.
  • Yamane, S., Kudô, K., Tajima, T., Nihon’yanagi, K., Shinoda, M., Saito, K., Yamamoto, H., 1998. Comparison of investment in nest construction by the foundresses of consubgeneric Polistes wasps, P. (polistes) riparius and P. chinensis (Hymenoptera: Vespidae). Journal of Ethology., 16(2): 97-104.
  • Yıldırım, E., Özbek, H., 1992. Türkiye Vespinae (Hymenoptera: Vespoidea: Vespidae) türleri üzerinde sistematik ve faunistik çalışmalar. Türk Entomoloji Derg., 16(4): 227-242.

Vespa crabro germana Christ, 1791 (Hymenoptera: Vespidae)’in Yuva Materyalinin Kimyasal Bileşenleri, Antimikrobiyal ve Antioksidan Aktiviteleri

Year 2020, Volume: 51 Issue: 1, 44 - 52, 25.01.2020
https://doi.org/10.17097/ataunizfd.593016

Abstract

Sosyal eşekarısı, yuvalarını doğada çeşitli organik
ve inorganik malzemeler kullanarak oluşturur. Bu çalışmada,
Vespa crabro germana Christ, 1791
yuvasına ait etanol ekstresinin toplam fenolik madde (TFM) içeriği, ferrik iyon
indirgeyici antioksidan gücü (FRAP) ve DPPH radikal süpürme etkisine dayalı
antioksidan potansiyeli, biyokimyasal bileşimi ve antimikrobiyal aktivitesi araştırılmıştır.
Disk difüzyon yöntemi kullanılarak 8 bakteri ve 2 mantar türüne karşı antibakteriyel
ve antifungal aktivite belirlenmiştir. İncelenen yuva numunesinin etanol
ekstresi,
Proteus vulgaris, Bacillus subtillis, Micrococcus luteus ve Candida
albicans'
a karşı maksimum antimikrobiyal aktivite göstermiştir. Öte yandan,
Pseudomonas aeruginosa, Yersinia enterocolitica, Aspergillus niger ve Klebsiella pneumoniae'ye karşı minimum
aktivite elde edilmiştir. Örnek ekstraktının TFM içeriği Folin-Ciocalteu testi
kullanılarak analiz edilmiş ve 0.56 mg gallik asit eşdeğeri (GAE)/g numune
olarak hesaplanmıştır. Ekstrenin FRAP değeri ve DPPH radikal süpürme aktivitesi
sırasıyla 1.94 mg troloks eşdeğeri (TE)/numune ve 0.88 mg TE/g numune olarak tespit
edilmiştir. Numunenin GC-MS analizi katı faz mikroekstraksiyon (SPME) tekniğine
göre yapılmış ve 44 bileşik tanımlanmıştır. Biyoaktif potansiyeli olan bu
bileşiklerin varlığı, araştırılan numune ekstraktına, antifungal,
antibakteriyel, antiviral ajanlar vb. gibi farmasötik anlamda katma değer sağlar.
Başka bir ifadeyle, yuva numunesinin antioksidan ve antimikrobiyal potansiyeli,
bu bileşiklerin varlığına atfedilebilir. Bu nedenle sosyal eşekarısı tarafından
yavru yetiştiriciliği için kullanılan yuvaların yapısında kullanılan malzemeler
 biyolojik aktiviteler açısından önem arz
eder.

References

  • Akyüz, E., Şahin, H., Islamoglu, F., Kolayli, S., Sandra, P., 2014. Evaluation of phenolic compounds in Tilia rubra subsp. caucasica by HPLC-UV and HPLC-UV-MS/MS. International Journal of Food Properties., 17(2): 331-343.
  • Anderson, K.E., Sheehan, T.H., Eckholm, B.J., Mott, B.M., DeGrandi-Hoffman, G. 2011. An emerging paradigm of colony health: microbial balance of the honey bee and hive (Apis mellifera). Insectes Sociaux., 58 (4): 431.
  • Bagriacik, N., 2011. Determination of some structural features of the nest paper of Vespa orientalis Linneaus, 1771 and Vespa crabro Linneaus, 1758 (Hymenoptera: Vespinae) in Turkey. Archives of Biological Sciences., 63(2): 449-455.
  • Banks, B.E., Shipolini, R.A., 1986. Chemistry and pharmacology of honey-bee venom. Venoms of the Hymenoptera: Biochemical, pharmacological and behavioural aspects., 330-416.
  • Benzie, I.F., Strain, J.J., 1996. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Analytical biochemistry., 239(1): 70-76.
  • Blum, M.S., Walker, J.R., Callahan, P.S., & Novak, A.F., 1958. Chemical, insecticidal, and antibiotic properties of fire ant venom. Science., 128(3319): 306-307.
  • Christe, P., Oppliger, A., Bancalà, F., Castella, G., Chapuisat, M., 2003. Evidence for collective medication in ants. Ecology Letters., 6(1): 19-22.
  • Ertürk, Ö., Kati, H., Yayli, N., Demirbağ, Z., 2003. Antimicrobial activity of Viscum album L. subsp. abietis (Wiesb). Turkish Journal of Biology., 27(4): 255-258.
  • Ertürk, Ö., Karakaş, F.P., Pehlivan, D., Nas, N., 2009. The antibacterial and antifungal effects of Rhododendron derived mad honey and extracts of four Rhododendron species. Turkish Journal of Biology., 33(2): 151-158.
  • Jeanne, R.L., 1977. Ultimate factors in social wasp nesting behavior. Proceedings of the International Congress of the International Union for the Study of Social Insects., 164-168.
  • Jeanne, R.L., 1986. The organization of work in Polybia occidentalis: costs and benefits of specialization in a social wasp. Behavioral Ecology and Sociobiology., 19(5): 333-341.
  • Jeanne, R.L., 1996. The evolution of exocrine gland function in wasps. Natural History and Evolution of Paper Wasps., 145-160.
  • Langenheim, J.H., 2003. Plant resins: chemistry, evolution, ecology, and ethnobotany (No. 620.1924 L275p). Oregon, US: Timber Press.
  • Lokvam, J., Braddock, J.F., 1999. Anti-bacterial function in the sexually dimorphic pollinator rewards of Clusia grandiflora (Clusiaceae). Oecologia., 119(4): 534-540.
  • Maschwitz, U., Dorow, W.H.O., Botz, T., 1990. Chemical composition of the nest walls, and nesting behaviour, of Ropalidia (Icarielia) opifex van der Vecht, 1962 (Hymenoptera: Vespidae), a Southeast Asian social wasp with translucent nests. Journal of Natural History., 24(5): 1311-1319.
  • McGovern, J.N., Jeanne, R.L., Effland, M.J., 1988. The nature of wasp nest paper. Tappi Journal., 71: 133-139.
  • Monteiro, M.C., Romão, P.R., Soare, A.M., 2009. Pharmacological perspectives of wasp venom. Protein and peptide letters., 16(8): 944-952.
  • Ronald, M.A., 1990. Microbiologia. Compania editorial continental SA de CV, Mexico, DF, 505.
  • Rosengaus, R.B., Guldin, M.R., Traniello, J.F., 1998. Inhibitory effect of termite fecal pellets on fungal spore germination. Journal of Chemical Ecology., 24(10): 1697-1706.
  • Rukaiyat, M., Garba, S., Labaran, S., 2015. Antimicrobial activities of hexacosane isolated from Sanseveria liberica (Gerome and Labroy) plant. Adv Med Plant Res., 3(3): 120-125.
  • Schmid-Hempel, P., 1998. Parasites in social insects. Princeton University Press.
  • Schremmer, F., Marz, L., Simonsberger, P., 1985. Chitin im Speichel der Papierwespen (soziale Faltenwespen, Vespidae): Biologie, Chemismus, Feinstruktur. Mikroskopie., 42(1-2): 52-56.
  • Silva, T.M.S., Camara, C.A., da Silva Lins, A.C., Barbosa-Filho, J.M., da Silva, E.M.S., Freitas, B.M., dos Santos, F.D.A.R., 2006. Chemical composition and free radical scavenging activity of pollen loads from stingless bee Melipona subnitida Ducke. Journal of food composition and analysis., 19(6-7): 507-511.
  • Singleton, V.L., Rossi, J.A., 1965. Colorimetry of total phenolics with phosphomolybdic phosphotungstic acid reagents. Am J Enol Viticult., 16: 144-158.
  • Smania, E.F.A., Monoache, F.D., Yunes, R.A., Pauler, R., Junior, A.S., 2007. Antimicrobial activity of methyl australate from Ganoderma australe. Brazilian Journal of Pharmacognosy., 17(1): 14-16.
  • Spradbery, J.P., 1973. Wasps. An account of the biology and natural history of social and solitary wasps, with particular reference to those of the British Isles.
  • Starr, C.K., 1991. The nest as the locus of social life. The social biology of wasps., 520-539.
  • Subbaiyan, B., Samydurai, P., KarthikPrabu, M., Thangapandian, V., 2014. Gas chromatography and mass spectrum analysis of Catharanthus pusillus (Murray) g. Don (Apocyanaceae). International Research Journal of Pharmaceutical and Applied Sciences (IRJPAS)., 4(2): 4.
  • Tamil Muthu, P., Selvaraj, D., 2015. Analysis of Bioactive Constituents from the Flesh of Turbo brunneus (Roding, 1798) by GCMS. International Journal of Fisheries and Aquatic Studies., 3(1): 257-259.
  • Yamane, S., Kudô, K., Tajima, T., Nihon’yanagi, K., Shinoda, M., Saito, K., Yamamoto, H., 1998. Comparison of investment in nest construction by the foundresses of consubgeneric Polistes wasps, P. (polistes) riparius and P. chinensis (Hymenoptera: Vespidae). Journal of Ethology., 16(2): 97-104.
  • Yıldırım, E., Özbek, H., 1992. Türkiye Vespinae (Hymenoptera: Vespoidea: Vespidae) türleri üzerinde sistematik ve faunistik çalışmalar. Türk Entomoloji Derg., 16(4): 227-242.
There are 31 citations in total.

Details

Primary Language Turkish
Journal Section ARAŞTIRMALAR
Authors

Ömer Ertürk 0000-0001-5837-6893

Atilla Şimşek This is me 0000-0003-2092-1803

Publication Date January 25, 2020
Published in Issue Year 2020 Volume: 51 Issue: 1

Cite

APA Ertürk, Ö., & Şimşek, A. (2020). Vespa crabro germana Christ, 1791 (Hymenoptera: Vespidae)’in Yuva Materyalinin Kimyasal Bileşenleri, Antimikrobiyal ve Antioksidan Aktiviteleri. Atatürk Üniversitesi Ziraat Fakültesi Dergisi, 51(1), 44-52. https://doi.org/10.17097/ataunizfd.593016
AMA Ertürk Ö, Şimşek A. Vespa crabro germana Christ, 1791 (Hymenoptera: Vespidae)’in Yuva Materyalinin Kimyasal Bileşenleri, Antimikrobiyal ve Antioksidan Aktiviteleri. Atatürk Üniversitesi Ziraat Fakültesi Dergisi. January 2020;51(1):44-52. doi:10.17097/ataunizfd.593016
Chicago Ertürk, Ömer, and Atilla Şimşek. “Vespa Crabro Germana Christ, 1791 (Hymenoptera: Vespidae)’in Yuva Materyalinin Kimyasal Bileşenleri, Antimikrobiyal Ve Antioksidan Aktiviteleri”. Atatürk Üniversitesi Ziraat Fakültesi Dergisi 51, no. 1 (January 2020): 44-52. https://doi.org/10.17097/ataunizfd.593016.
EndNote Ertürk Ö, Şimşek A (January 1, 2020) Vespa crabro germana Christ, 1791 (Hymenoptera: Vespidae)’in Yuva Materyalinin Kimyasal Bileşenleri, Antimikrobiyal ve Antioksidan Aktiviteleri. Atatürk Üniversitesi Ziraat Fakültesi Dergisi 51 1 44–52.
IEEE Ö. Ertürk and A. Şimşek, “Vespa crabro germana Christ, 1791 (Hymenoptera: Vespidae)’in Yuva Materyalinin Kimyasal Bileşenleri, Antimikrobiyal ve Antioksidan Aktiviteleri”, Atatürk Üniversitesi Ziraat Fakültesi Dergisi, vol. 51, no. 1, pp. 44–52, 2020, doi: 10.17097/ataunizfd.593016.
ISNAD Ertürk, Ömer - Şimşek, Atilla. “Vespa Crabro Germana Christ, 1791 (Hymenoptera: Vespidae)’in Yuva Materyalinin Kimyasal Bileşenleri, Antimikrobiyal Ve Antioksidan Aktiviteleri”. Atatürk Üniversitesi Ziraat Fakültesi Dergisi 51/1 (January 2020), 44-52. https://doi.org/10.17097/ataunizfd.593016.
JAMA Ertürk Ö, Şimşek A. Vespa crabro germana Christ, 1791 (Hymenoptera: Vespidae)’in Yuva Materyalinin Kimyasal Bileşenleri, Antimikrobiyal ve Antioksidan Aktiviteleri. Atatürk Üniversitesi Ziraat Fakültesi Dergisi. 2020;51:44–52.
MLA Ertürk, Ömer and Atilla Şimşek. “Vespa Crabro Germana Christ, 1791 (Hymenoptera: Vespidae)’in Yuva Materyalinin Kimyasal Bileşenleri, Antimikrobiyal Ve Antioksidan Aktiviteleri”. Atatürk Üniversitesi Ziraat Fakültesi Dergisi, vol. 51, no. 1, 2020, pp. 44-52, doi:10.17097/ataunizfd.593016.
Vancouver Ertürk Ö, Şimşek A. Vespa crabro germana Christ, 1791 (Hymenoptera: Vespidae)’in Yuva Materyalinin Kimyasal Bileşenleri, Antimikrobiyal ve Antioksidan Aktiviteleri. Atatürk Üniversitesi Ziraat Fakültesi Dergisi. 2020;51(1):44-52.

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