Chemical Components, Antimicrobial and Antioxidant Activities of Nest Materials Vespa crabro germana Christ, 1791 (Hymenoptera: Vespidae)

Year 2020, Volume: 51 Issue: 1, 44 - 52, 25.01.2020

Ömer Ertürk , Atilla Şimşek

https://doi.org/10.17097/ataunizfd.593016

Abstract

Social wasps build their nests in nature by using
various organic and inorganic materials. In this study, the total phenolic
content, antioxidant potentials based on ferric reducing antioxidant power
(FRAP) and DPPH radical scavenging activity, biochemical composition and
antimicrobial activity of the ethanol extract of Vespa crabro germana Christ,
1791 nest were investigated. Antibacterial and antifungal activity was
determined by disc diffusion method against 8 bacteria and 2 fungi species. The
ethanol extract of the investigated nest sample showed maximum antimicrobial
activities against Proteus vulgaris, Bacillus
subtillis, Micrococcus luteus and Candida
albicans. On the other hand, the minimum activities obtained against Pseudomonas aeruginosa, Yersinia
enterocolitica, Aspergillus niger and Klebsiella
pneumoniae. Total phenolic content (TPC) of sample extract was analyzed
using Folin–Ciocalteu assay and calculated as 0.56 mg gallic acid equivalent/g
sample. FRAP value and DPPH radical scavenging activity of the extract value were
determined as 1.94 mg trolox equivalent/g sample and 0.88 mg trolox equivalent/g
sample, respectively. GC-MS analysis of the sample was according to solid phase
microextraction (SPME) technique and 44 compounds were identified. The presence
of these compounds with bioactive potential, add value to investigated sample
extract with a pharmaceutical meaning as antifungal, antibacterial, antiviral
agents and so on. In other words, the antioxidative and antimicrobial potantial
of the nest sample could be attributed to presence of these compounds. For this
reason, the materials used in the structure of the nests used for breeding by
the social wasp are important for the biological activities.

Keywords

Vespa crabro germana, FRAP, Antibacterial, Antifungal

Vespa crabro germana Christ, 1791 (Hymenoptera: Vespidae)’in Yuva Materyalinin Kimyasal Bileşenleri, Antimikrobiyal ve Antioksidan Aktiviteleri

Abstract

Sosyal eşekarısı, yuvalarını doğada çeşitli organik
ve inorganik malzemeler kullanarak oluşturur. Bu çalışmada, Vespa crabro germana Christ, 1791
yuvasına ait etanol ekstresinin toplam fenolik madde (TFM) içeriği, ferrik iyon
indirgeyici antioksidan gücü (FRAP) ve DPPH radikal süpürme etkisine dayalı
antioksidan potansiyeli, biyokimyasal bileşimi ve antimikrobiyal aktivitesi araştırılmıştır.
Disk difüzyon yöntemi kullanılarak 8 bakteri ve 2 mantar türüne karşı antibakteriyel
ve antifungal aktivite belirlenmiştir. İncelenen yuva numunesinin etanol
ekstresi, Proteus vulgaris, Bacillus subtillis, Micrococcus luteus ve Candida
albicans'a karşı maksimum antimikrobiyal aktivite göstermiştir. Öte yandan,
Pseudomonas aeruginosa, Yersinia enterocolitica, Aspergillus niger ve Klebsiella pneumoniae'ye karşı minimum
aktivite elde edilmiştir. Örnek ekstraktının TFM içeriği Folin-Ciocalteu testi
kullanılarak analiz edilmiş ve 0.56 mg gallik asit eşdeğeri (GAE)/g numune
olarak hesaplanmıştır. Ekstrenin FRAP değeri ve DPPH radikal süpürme aktivitesi
sırasıyla 1.94 mg troloks eşdeğeri (TE)/numune ve 0.88 mg TE/g numune olarak tespit
edilmiştir. Numunenin GC-MS analizi katı faz mikroekstraksiyon (SPME) tekniğine
göre yapılmış ve 44 bileşik tanımlanmıştır. Biyoaktif potansiyeli olan bu
bileşiklerin varlığı, araştırılan numune ekstraktına, antifungal,
antibakteriyel, antiviral ajanlar vb. gibi farmasötik anlamda katma değer sağlar.
Başka bir ifadeyle, yuva numunesinin antioksidan ve antimikrobiyal potansiyeli,
bu bileşiklerin varlığına atfedilebilir. Bu nedenle sosyal eşekarısı tarafından
yavru yetiştiriciliği için kullanılan yuvaların yapısında kullanılan malzemeler
 biyolojik aktiviteler açısından önem arz
eder.

Keywords

Vespa crabro germana, FRAP, Antibakteriyel, Antifungal

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