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Bioinformatics of Glutathione Reductase Gene of Platy Fish, Xiphophorus maculatus Günther, 1866

Year 2020, Volume: 51 Issue: 2, 159 - 167, 19.05.2020
https://doi.org/10.17097/ataunizfd.631335

Abstract

In this study, the genomic organization of glutathione reductase (gsr) gene was determined in platyfish, Xiphophorus maculatus which is an important model organism. For this purpose, we studied bioinformatics of glutathione reductase gene (gsr) (gene structure andidentification of gene, detecting of amino acid sequences encoded by other vertebrates, conserved gene synteny and phylogenetic relationships with other vertebrates) in platyfish, X. maculatus. However, bioinformatic studies of the gsr enzyme gene have not been performed in platyfish which is an important model organism. For all these purposes, in this study, the statistics obtained by using Ensembl genomic database, NCBI database, BioEdit software and MEGA6 program are presented. Stress responses in fish can reveal multifaceted levels involving the effects of different gene groups and products. Understanding the genetic characteristics of a model organism that reacts to stress is crucial for molecular studies. For this purpose, the characterization and identification of gsr, one of the antioxidant enzyme genes in platyfish, X. macullastus which is an important model organism, was made using bioinformatics tools and some data to be used in the future studies on the molecular stress response were presented to the scientific world. 

References

  • Amores, A., Force A., Yan Y. L., Joly L., Amemiya C., Fritz A., Ho R. K., Langeland J., Prince V., Wang Y.L., 1998. Zebrafish hox clusters and vertebrate genome evolution. Science 282, 1711-1714.
  • Arthington, A.H., 1989. Diet of G. affinis holbrooki, Xiphophorus helleri, X. maculatus and Poecilia reticulata (Pisces:Poeciliidae) in streams of southeastern Qeennsland, Australia. Asian Fish Sci. 2(2):193-212.
  • Atalay, R.Ç, 2002. Neden Biyoinformatik?, Avrasya Dosyası,Moleküler Biyoloji ve Gen Teknolojileri Özel, Sonbahar, Cilt 8,Sayı:3,129-141.
  • Attwood T.K., Parry-Smith D.J.,1999. Introduction to Bioinformatics, Prentice Hall, Harlow, 240.
  • Aydemir, B., Karadağ-Sarı E., 2009. Antioksidanlar ve Büyüme Faktörleri ile İlişkisi. Kocatepe Veterinary Journal, 2 (2): 56-60.
  • Baxevanis, A.D., 2001. Ouellette, B. F. F., Bioinformatics: A Practical Guide to the Analysis of Genes and Proteins, 2nd Edition, John Wiley & Sons Inc., New York (NY), 356.
  • Bonsignore, A., Fornaini, G., Leoncini, G., Fontani, A., Segni, P., 1966. Characterization of leucocyte glucose 6 phoshate dehydrogenase in Sardinian Mutants. J. Clin. Invest. 45, 12-16.
  • Bonsignore, A., De Flora, A., 1972. Regulatory properties of glocose 6-phosphate dehydrogenase. Curr. Top. Cell. 6, 21-62.
  • Buechter, D. D., 1988. Free radicals and oxygen toxicity. Pharmaceutical Research. 5 (5), 253-260.Boswell M.G., Wells M.C., Kirk L.M., Ju Z., Zhang Z., Booth R.E.,
  • Dougall, W.C., 1991. Nick HS. Manganese superoxide dismutase: a hepatic acute phase protein regulated by interleukin-6 and glucocorticoids. Endocrinology, 129:2376–2384.
  • Felsenstein, J., 1989. PHYLIP-Phylogeny inference package. Cladistics, 5, 164-166.
  • Gromiha M.M., 2010. Protein Bioinformatics: From Sequence to Function.
  • Gutteridge, J.M.C., 1993. “Free radicals in disease processes: a compilation of cause and consequence,” Free Radical Research Communications, vol. 19, no.3, pp. 141–158.
  • Halliwell, B., 1991. Reactive oxygen species in living systems: Source, biochemistry, and role in human disease. The American Journal of Medicine, 91(3), 14–22.
  • Iwama, G.K., Vıjayan A.M., Forsyth B., Ackerman P.A., 1999. Heat Shock Proteins and Physiological Stress in Fish. AMER. ZOOL., 39:901-909.
  • Jones, P.L., Ping D., Boss J.M., 1997. Tumor necrosis factor alpha and interleukin-1b regulate the murine manganese superoxide dismutase gene through a complex intronic enhancer involving C/EBP-b and NF-jB. Mol Cell Biol, 17: 6970–6981.
  • Kallman, K.D., Walter R.B., Morizot D.C., Kazianis S., 2004. " Two new species of Xiphophorus (Poeciliidae), from the Isthmus of Tehuantepec, Oaxaca, Mexico, with a Discussion of the Distribution of the X. clemenciae Clade." American Museum Novitates, 3441:1-34.
  • Keha, E., Küfrevioğlu Ö.İ., 1997. Enzimler, Biyokimya Kitabı, 2.Baskı, 97-98, Şafak Yayınevi, Erzurum.
  • Kell A.J.E., Yamins D.L.K., Shook E.N., Norman-Haignere S.V., 2018. A Task-Optimized Neural Network Replicates Human Auditory Behavior, Predicts Brain Responses, and Reveals a Cortical Processing Hierarchy. Neron Volume 98, Issue 3, Pages 630-644.e16.
  • Ken, C.F., LinC.T., Wu J.L., Shaw J.F., 2000. Zebra balığı kaynaklı (Danio rerio) katalaz kodlayan bir cDNA'nın klonlanması ve ifadesi. J Agric Food Chem. Jun, 48 (6): 2092-6.
  • Mahdi, A.A., Shukla K.K., Ahmad M.K., Rajender S., Shankhwar S.N., Singh V., Dalela D., 2011. Withania somnifera Improves Semen Quality in Stress-RelatedMale Fertility. Hindawi Pub. Corp. Evidence-Based Comp. and Alt. Med.. Article ID 576962, 9.
  • Mckenzie, F.T., Bischoff W.D., Bishop F.C., Loijens M., Schoonmaker J., Wollast R., 1983. Magnesian calcites: low temperature occurrence, solubility and solid-solution behavior. In: Carbonates: Mineralogy and Chemistry. Reviews in Mineralogy, vol. 11 (ed. Reeder, R.J.). Mineralogical Society of America, Washington, DC, 97–143.
  • Poljsak, B., Šuput D., Milisav I., 2013. Achieving the Balance between ROS and Antioxidants: When to Use the Synthetic Antioxidants. Hindawi Publishing Corporation Oxidative Med. and Cellular Longevity, Article ID 956792, 11 .
  • Postlethwait, J. H., Woods, I. G., Ngo-Hazelett, P., Yan, Y. L., Kelly, P. D., Chu, F., Huang, H., Hill-Force, A. and Talbot, W. S. 2000. Zebrafish comparative genomics and the origins of vertebrate chromosomes. Genome Res. 10, 1890-1902.
  • Rogers, R.J., 2000. Chesrown SE, Kuo S et al. Cytokine-inducible enhancer with promoter activity in both the rat and human manganese-superoxide dismutase genes. Biochem J, 347:233–242.Schartl, M., 2014. Beyond the zebrafish: diverse fish species for modeling human Disease. Published by The Company of Biologists Ltd | Dis. Models & Mech. 7, 181-192.
  • Schartl, M., Walter R.B., Shen Y., Garcia T., Catchen J., Amores A., Braasch I., Chalopin D., Volff J.N., Lesch K.P., Bisazza A., Minx P., Hillier L., Wilson R.K., Fuerstenberg S., Boore J., Searle S., Postlethwait J.H., Warren W.C., 2014. The genome of the platyfish, Xiphophorus maculatus, provides insights into evolutionary adaptation and several complex traits. Nat Genet. 2013 May ; 45 (5): 567–572. doi:10.1038/ng.2604.
  • Schreibman, M.P., Kallman K. D., 1978. The genetic control of sexual maturation in the teleost, Xiphophorus maculatus (Poeciliidae) ; a review. Biology Department, Brooklyn College of the City University of New York, 11210, Osborn Laboratories of Marine Sciences, New York Aquarium, Brooklyn, New York 11203, USA. Ann.biol. anim.bioch.biophys. 18(4),957-962.Sen, S., 2011. Chakraborty R. The Role of Antioxidants in Human Health. American Chemical Society, Oxidative Stress: Diagnostics, Prevention and Therapy. Chapter 1: 1-37.
  • Shinde, A., Ganu J., Naik P., 2012. Effect of free radicals & Antioxidants on oxidative stress: A Review. J Dent Allied Sci, 1 (2): 6366.
  • Sugino, N., Hirosawa-Takamori M., Zhong L., 1998. Hormonal regulation of copper-zinc superoxide dismutase and manganese superoxide dismutase messenger ribonucleic acid in the rat corpus luteum: induction by prolactin and placental lactogens. Biol Rept, 59:599–605.
  • Sun, H.J., Wang W.Q., Geng L.L., Chen Y.F., Yang Z., 2013. In situ studies on growth, oxidative stress responses, and gene expression of juvenile bighead carp (Hypophthalmichthys nobilis) to eutrophic lake water dominated by cyanobacterial blooms, Chemosphere, 93, 2, 421-427.
  • Sun, Y., 1990. Free radicals, antioxidant enzymes, and carcinogenesis. Free Rad. Biol. Med. 8, 583–599.
  • Tamura, K., Stecher G., Peterson D., Filipski A., Sudhir Kumar Tamura K., Stecher G., Peterson D., Filipski A., Kumar S., 2013. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0 Molecular Biology and Evolution, Volume 30,. doi:10.1093/molbev/mst197.
  • Tandon, P., Bhattacharjee P., 2009. Bioinformatics: An overview, Plant Tissue Culture and Molecular Markers, I.K. International Publishing House Pvt. Ltd., Green Park Extension Uphaar Cinema Market, New Delhi, India, 29-49.
  • Thompson, J.D., Higgins D.G., Gibson T.J., 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice , Nucleic Acids Res., 22, pp. 4673-4680.
  • Volff, J.N., Bouneau L., Ozouf-Costaz C., 2003. Fischer C. Diversity of retrotransposable elements in compact pufferfish genomes. Trends in genetics: TIG. 19:674–8.
  • Walter R.B., 2009. Comparison of gene expression responses to hypoxia in viviparous (Xiphophorus) and oviparous (Oryzias) fishes using a medaka microarray. Comparative biochemistry and physiology c-toxicology&pharmacology. 149 (2): 2558-265.Braasch, I. and Postlethwait, J. H. I. 2012. Polyploidy in fish and the teleost genome duplication. In Polyploidy and Genome Evolution (ed. P. S. Soltis and D. E. Soltis), pp. 341-383: Springer.
  • Zaret, T.M.,1984. Evolutionary ecology of neotropical freshwater fishes. Proceedings of the 1st international symposium on systematics and evolutionary ecology of neotropical freshwater fishes, held at DeKalb, Illinois, U.S.A., Publisher: 978-90-481-8524-5. Buechter, D. D., 1988. Free radicals and oxygen toxicity. Pharmaceutical Res. (5), 253-260.

Plati Balığı, Xiphophorus maculatus Günther, 1866’nda Glutatyon Redüktaz Geninin Biyoenformatiği

Year 2020, Volume: 51 Issue: 2, 159 - 167, 19.05.2020
https://doi.org/10.17097/ataunizfd.631335

Abstract

Bu çalışmada, plati balığı, Xiphophorus maculatus’ta antioksidan enzim genlerinden, glutatyon redüktaz (gsr)’ın genomik organizasyonu belirlenmiştir. Bu amaçla genin tanımlanması yapılmış, ekzon- intron organizasyonu, TATA kutusu, poli A kuyruğu ve genin üretmiş olduğu amino asitler belirlenerek gen yapısı bir tablo şeklinde sunulmuş, gsr gen kromozom bölgeleri tespit edilmiştir.Ayrıca bu bölgede bulunan genlerin, diğer bazı model organizmalarda ve insanda bulunduğu gen bölgeleri belirlenerek korunmuş gen sentezi oluşturulmuş, diğer omurgalılar tarafından kodlanan amino asit dizileri belirlenmiş ve bu organizmalarla olan filogenetik ilişkitespit edilerek, gen yapılarının benzerlik-özdeşlik oranlarının % olarak değeri belirlenmiştir. Çalışmada NCBI veritabanı, Ensembl genomik veritabanı, MEGA 6 programı ve BioEdit yazılımı kullanılarak elde edilen istatistikler değerlendirilmiştir. Balıkların stress faktörlerine göstermiş oldukları tepkiler, farklı gen ürünlerinin etkilerini içeren çok yönlü seviyelerin ortaya çıkmasına neden olabilir. Dolayısıyla moleküler çalışmalarla ölçülebilecek bir balık stress tepkisive bu balığın genetik özelliklerinin araştırılması ve anlaşılması moleküler çalışmalar açısından büyük önem taşıdığı için, biyoenformatik araçlar kullanarak önemli bir model organizma olan plati balığı, X. maculatus’ta, gsr’nin karakterizasyonu ve tanımlanması yapılmış ve balıklarda moleküler stres tepkisi üzerine yapılacak olan çalışmalarda kullanılacak birtakım veriler bu çalışma ile bilim dünyasına sunulmuştur.

References

  • Amores, A., Force A., Yan Y. L., Joly L., Amemiya C., Fritz A., Ho R. K., Langeland J., Prince V., Wang Y.L., 1998. Zebrafish hox clusters and vertebrate genome evolution. Science 282, 1711-1714.
  • Arthington, A.H., 1989. Diet of G. affinis holbrooki, Xiphophorus helleri, X. maculatus and Poecilia reticulata (Pisces:Poeciliidae) in streams of southeastern Qeennsland, Australia. Asian Fish Sci. 2(2):193-212.
  • Atalay, R.Ç, 2002. Neden Biyoinformatik?, Avrasya Dosyası,Moleküler Biyoloji ve Gen Teknolojileri Özel, Sonbahar, Cilt 8,Sayı:3,129-141.
  • Attwood T.K., Parry-Smith D.J.,1999. Introduction to Bioinformatics, Prentice Hall, Harlow, 240.
  • Aydemir, B., Karadağ-Sarı E., 2009. Antioksidanlar ve Büyüme Faktörleri ile İlişkisi. Kocatepe Veterinary Journal, 2 (2): 56-60.
  • Baxevanis, A.D., 2001. Ouellette, B. F. F., Bioinformatics: A Practical Guide to the Analysis of Genes and Proteins, 2nd Edition, John Wiley & Sons Inc., New York (NY), 356.
  • Bonsignore, A., Fornaini, G., Leoncini, G., Fontani, A., Segni, P., 1966. Characterization of leucocyte glucose 6 phoshate dehydrogenase in Sardinian Mutants. J. Clin. Invest. 45, 12-16.
  • Bonsignore, A., De Flora, A., 1972. Regulatory properties of glocose 6-phosphate dehydrogenase. Curr. Top. Cell. 6, 21-62.
  • Buechter, D. D., 1988. Free radicals and oxygen toxicity. Pharmaceutical Research. 5 (5), 253-260.Boswell M.G., Wells M.C., Kirk L.M., Ju Z., Zhang Z., Booth R.E.,
  • Dougall, W.C., 1991. Nick HS. Manganese superoxide dismutase: a hepatic acute phase protein regulated by interleukin-6 and glucocorticoids. Endocrinology, 129:2376–2384.
  • Felsenstein, J., 1989. PHYLIP-Phylogeny inference package. Cladistics, 5, 164-166.
  • Gromiha M.M., 2010. Protein Bioinformatics: From Sequence to Function.
  • Gutteridge, J.M.C., 1993. “Free radicals in disease processes: a compilation of cause and consequence,” Free Radical Research Communications, vol. 19, no.3, pp. 141–158.
  • Halliwell, B., 1991. Reactive oxygen species in living systems: Source, biochemistry, and role in human disease. The American Journal of Medicine, 91(3), 14–22.
  • Iwama, G.K., Vıjayan A.M., Forsyth B., Ackerman P.A., 1999. Heat Shock Proteins and Physiological Stress in Fish. AMER. ZOOL., 39:901-909.
  • Jones, P.L., Ping D., Boss J.M., 1997. Tumor necrosis factor alpha and interleukin-1b regulate the murine manganese superoxide dismutase gene through a complex intronic enhancer involving C/EBP-b and NF-jB. Mol Cell Biol, 17: 6970–6981.
  • Kallman, K.D., Walter R.B., Morizot D.C., Kazianis S., 2004. " Two new species of Xiphophorus (Poeciliidae), from the Isthmus of Tehuantepec, Oaxaca, Mexico, with a Discussion of the Distribution of the X. clemenciae Clade." American Museum Novitates, 3441:1-34.
  • Keha, E., Küfrevioğlu Ö.İ., 1997. Enzimler, Biyokimya Kitabı, 2.Baskı, 97-98, Şafak Yayınevi, Erzurum.
  • Kell A.J.E., Yamins D.L.K., Shook E.N., Norman-Haignere S.V., 2018. A Task-Optimized Neural Network Replicates Human Auditory Behavior, Predicts Brain Responses, and Reveals a Cortical Processing Hierarchy. Neron Volume 98, Issue 3, Pages 630-644.e16.
  • Ken, C.F., LinC.T., Wu J.L., Shaw J.F., 2000. Zebra balığı kaynaklı (Danio rerio) katalaz kodlayan bir cDNA'nın klonlanması ve ifadesi. J Agric Food Chem. Jun, 48 (6): 2092-6.
  • Mahdi, A.A., Shukla K.K., Ahmad M.K., Rajender S., Shankhwar S.N., Singh V., Dalela D., 2011. Withania somnifera Improves Semen Quality in Stress-RelatedMale Fertility. Hindawi Pub. Corp. Evidence-Based Comp. and Alt. Med.. Article ID 576962, 9.
  • Mckenzie, F.T., Bischoff W.D., Bishop F.C., Loijens M., Schoonmaker J., Wollast R., 1983. Magnesian calcites: low temperature occurrence, solubility and solid-solution behavior. In: Carbonates: Mineralogy and Chemistry. Reviews in Mineralogy, vol. 11 (ed. Reeder, R.J.). Mineralogical Society of America, Washington, DC, 97–143.
  • Poljsak, B., Šuput D., Milisav I., 2013. Achieving the Balance between ROS and Antioxidants: When to Use the Synthetic Antioxidants. Hindawi Publishing Corporation Oxidative Med. and Cellular Longevity, Article ID 956792, 11 .
  • Postlethwait, J. H., Woods, I. G., Ngo-Hazelett, P., Yan, Y. L., Kelly, P. D., Chu, F., Huang, H., Hill-Force, A. and Talbot, W. S. 2000. Zebrafish comparative genomics and the origins of vertebrate chromosomes. Genome Res. 10, 1890-1902.
  • Rogers, R.J., 2000. Chesrown SE, Kuo S et al. Cytokine-inducible enhancer with promoter activity in both the rat and human manganese-superoxide dismutase genes. Biochem J, 347:233–242.Schartl, M., 2014. Beyond the zebrafish: diverse fish species for modeling human Disease. Published by The Company of Biologists Ltd | Dis. Models & Mech. 7, 181-192.
  • Schartl, M., Walter R.B., Shen Y., Garcia T., Catchen J., Amores A., Braasch I., Chalopin D., Volff J.N., Lesch K.P., Bisazza A., Minx P., Hillier L., Wilson R.K., Fuerstenberg S., Boore J., Searle S., Postlethwait J.H., Warren W.C., 2014. The genome of the platyfish, Xiphophorus maculatus, provides insights into evolutionary adaptation and several complex traits. Nat Genet. 2013 May ; 45 (5): 567–572. doi:10.1038/ng.2604.
  • Schreibman, M.P., Kallman K. D., 1978. The genetic control of sexual maturation in the teleost, Xiphophorus maculatus (Poeciliidae) ; a review. Biology Department, Brooklyn College of the City University of New York, 11210, Osborn Laboratories of Marine Sciences, New York Aquarium, Brooklyn, New York 11203, USA. Ann.biol. anim.bioch.biophys. 18(4),957-962.Sen, S., 2011. Chakraborty R. The Role of Antioxidants in Human Health. American Chemical Society, Oxidative Stress: Diagnostics, Prevention and Therapy. Chapter 1: 1-37.
  • Shinde, A., Ganu J., Naik P., 2012. Effect of free radicals & Antioxidants on oxidative stress: A Review. J Dent Allied Sci, 1 (2): 6366.
  • Sugino, N., Hirosawa-Takamori M., Zhong L., 1998. Hormonal regulation of copper-zinc superoxide dismutase and manganese superoxide dismutase messenger ribonucleic acid in the rat corpus luteum: induction by prolactin and placental lactogens. Biol Rept, 59:599–605.
  • Sun, H.J., Wang W.Q., Geng L.L., Chen Y.F., Yang Z., 2013. In situ studies on growth, oxidative stress responses, and gene expression of juvenile bighead carp (Hypophthalmichthys nobilis) to eutrophic lake water dominated by cyanobacterial blooms, Chemosphere, 93, 2, 421-427.
  • Sun, Y., 1990. Free radicals, antioxidant enzymes, and carcinogenesis. Free Rad. Biol. Med. 8, 583–599.
  • Tamura, K., Stecher G., Peterson D., Filipski A., Sudhir Kumar Tamura K., Stecher G., Peterson D., Filipski A., Kumar S., 2013. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0 Molecular Biology and Evolution, Volume 30,. doi:10.1093/molbev/mst197.
  • Tandon, P., Bhattacharjee P., 2009. Bioinformatics: An overview, Plant Tissue Culture and Molecular Markers, I.K. International Publishing House Pvt. Ltd., Green Park Extension Uphaar Cinema Market, New Delhi, India, 29-49.
  • Thompson, J.D., Higgins D.G., Gibson T.J., 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice , Nucleic Acids Res., 22, pp. 4673-4680.
  • Volff, J.N., Bouneau L., Ozouf-Costaz C., 2003. Fischer C. Diversity of retrotransposable elements in compact pufferfish genomes. Trends in genetics: TIG. 19:674–8.
  • Walter R.B., 2009. Comparison of gene expression responses to hypoxia in viviparous (Xiphophorus) and oviparous (Oryzias) fishes using a medaka microarray. Comparative biochemistry and physiology c-toxicology&pharmacology. 149 (2): 2558-265.Braasch, I. and Postlethwait, J. H. I. 2012. Polyploidy in fish and the teleost genome duplication. In Polyploidy and Genome Evolution (ed. P. S. Soltis and D. E. Soltis), pp. 341-383: Springer.
  • Zaret, T.M.,1984. Evolutionary ecology of neotropical freshwater fishes. Proceedings of the 1st international symposium on systematics and evolutionary ecology of neotropical freshwater fishes, held at DeKalb, Illinois, U.S.A., Publisher: 978-90-481-8524-5. Buechter, D. D., 1988. Free radicals and oxygen toxicity. Pharmaceutical Res. (5), 253-260.
There are 37 citations in total.

Details

Primary Language Turkish
Journal Section ARAŞTIRMALAR
Authors

Mehtap Bayır 0000-0002-7794-1058

Publication Date May 19, 2020
Published in Issue Year 2020 Volume: 51 Issue: 2

Cite

APA Bayır, M. (2020). Plati Balığı, Xiphophorus maculatus Günther, 1866’nda Glutatyon Redüktaz Geninin Biyoenformatiği. Atatürk Üniversitesi Ziraat Fakültesi Dergisi, 51(2), 159-167. https://doi.org/10.17097/ataunizfd.631335
AMA Bayır M. Plati Balığı, Xiphophorus maculatus Günther, 1866’nda Glutatyon Redüktaz Geninin Biyoenformatiği. Atatürk Üniversitesi Ziraat Fakültesi Dergisi. May 2020;51(2):159-167. doi:10.17097/ataunizfd.631335
Chicago Bayır, Mehtap. “Plati Balığı, Xiphophorus Maculatus Günther, 1866’nda Glutatyon Redüktaz Geninin Biyoenformatiği”. Atatürk Üniversitesi Ziraat Fakültesi Dergisi 51, no. 2 (May 2020): 159-67. https://doi.org/10.17097/ataunizfd.631335.
EndNote Bayır M (May 1, 2020) Plati Balığı, Xiphophorus maculatus Günther, 1866’nda Glutatyon Redüktaz Geninin Biyoenformatiği. Atatürk Üniversitesi Ziraat Fakültesi Dergisi 51 2 159–167.
IEEE M. Bayır, “Plati Balığı, Xiphophorus maculatus Günther, 1866’nda Glutatyon Redüktaz Geninin Biyoenformatiği”, Atatürk Üniversitesi Ziraat Fakültesi Dergisi, vol. 51, no. 2, pp. 159–167, 2020, doi: 10.17097/ataunizfd.631335.
ISNAD Bayır, Mehtap. “Plati Balığı, Xiphophorus Maculatus Günther, 1866’nda Glutatyon Redüktaz Geninin Biyoenformatiği”. Atatürk Üniversitesi Ziraat Fakültesi Dergisi 51/2 (May 2020), 159-167. https://doi.org/10.17097/ataunizfd.631335.
JAMA Bayır M. Plati Balığı, Xiphophorus maculatus Günther, 1866’nda Glutatyon Redüktaz Geninin Biyoenformatiği. Atatürk Üniversitesi Ziraat Fakültesi Dergisi. 2020;51:159–167.
MLA Bayır, Mehtap. “Plati Balığı, Xiphophorus Maculatus Günther, 1866’nda Glutatyon Redüktaz Geninin Biyoenformatiği”. Atatürk Üniversitesi Ziraat Fakültesi Dergisi, vol. 51, no. 2, 2020, pp. 159-67, doi:10.17097/ataunizfd.631335.
Vancouver Bayır M. Plati Balığı, Xiphophorus maculatus Günther, 1866’nda Glutatyon Redüktaz Geninin Biyoenformatiği. Atatürk Üniversitesi Ziraat Fakültesi Dergisi. 2020;51(2):159-67.

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