Mitigation of Drought Stress in Wheat by Bio-priming by PGPB Containing ACC Deaminase Activity

Year 2022, Volume: 53 Issue: 1, 51 - 57, 01.01.2022

Fatih Çığ Murat Erman Behçet İnal Harun Bektaş Mehmet Sonkurt Mohsen Mırzapour Mustafa Ceritoğlu

Abstract

Out of stress management strategies used for drought, inoculation of plant growth-promoting bacteria holds a major position due to sustainable, low-cost, and versatile properties. The plant growth-promoting bacteria, particularly containing 1-aminocyclopropane-1-carboxylic acid deaminase activity, have a critical location since they restrict ethylene synthesis under stress conditions thereby improving stress tolerance index. In this experiment, seeds of two wheat cultivars were primed with three bacterial strains and seedlings were grown under stress and nonstress conditions. The study was laid out in completely randomized factorial design with three replications. While plant growth achieved top performance with synthetic fertilizer in 80% of field capacity, increasing drought stress restricted the efficiency of synthetic fertilizer. In contrast, plant growth-promoting bacteria-priming promoted plant growth and dry matter accumulation under optimum and drought conditions. Increase of dry matter accumulation in treatments as control plants varied between 17.1% and 57.1% under 80% of field capacity while it changed between 0.2% and 35.1% under drought conditions. TV126C and TV24C induced stress tolerance index in sensitive and tolerant cultivars under drought and optimum conditions. In conclusion, it is considered that bio-priming with plant growth-promoting bacteria involving 1-aminocyclopropane-1-carboxylic acid deaminase enzyme activity might be an effective and sustainable management strategy to drought stress in wheat cultivation.

Keywords

Biological fertilizer, drought stress management, free-living microorganisms, seed priming, stress tolerance index, Triticum aestivum

Supporting Institution

Siirt University

Project Number

2017-SIUZIR-49

Thanks

This study was supported by the Scientific Research Projects Presidency of Siirt University.

ACC Deaminaz Aktivitesi İçeren PGPB Kullanılarak Biyo-priming ile Buğdayda Kuraklık Stresinin Azaltılması

Abstract

Kuraklığa karşı kullanılan stres yönetimi stratejileri arasında, bitki gelişimini teşvik edici bakterilerin (PGPB) inokulasyonu sürdürülebilir, düşük maliyetli ve çok yönlü özellikleri sayesinde önemli bir pozisyonda yer alır. Özellikle ACC (1-am inosi klopr opan- 1-kar boksi lik asit) aktivitesi içeren PGPB’ler stres koşulları altında etilen sentezini sınırlandırarak stres tolerans indeksini iyileştirdikleri için kritik bir pozisyona sahiptir. Bu deneyde, iki buğday çeşidinin tohumları üç bakteriyel strain ile ekim öncesinde inokule edildi ve fideler stres ve stres olmayan koşullar altında yetiştirildi. Çalışma tesadüf parsellerinde faktöriyel deneme desenine göre üç tekerrürlü olarak yürütülmüştür. Bitki gelişimi %80 tarla kapasitesinde sentetik gübreleme ile zirveye çıkarken artan kuraklık stresi sentetik gübrenin etkinliğini sınırlandırdı. Aksine, PGPB-priming hem optimum hem de kurak koşullarda bitki gelişimini ve kuru madde birikimini teşvik etti. Kontrol bitkilerine göre uygulamalarda kuru madde birikiminin artışı %80 tarla kapasitesinde %17.1–57.1 aralığında değişirken kurak koşullarda %0.2–35.1 aralığında değişmiştir. TV126C ve TV24C hassas ve dayanıklı çeşitlerde kuraklık stres toleransını teşvik etti. Sonuç olarak, ACC deaminaz aktivitesi içeren PGPB ile biyo-priming uygulamalarının buğday tarımında kuraklık stresine karşı etkili ve sürdürülebilir bir yönetim stratejisi olabileceği düşünülmektedir.

Keywords

Biyolojik gübre, kuraklık stres yönetimi, serbest yaşayan mikroorganizmalar, tohuma priming, stres tolerans indeksi, Triticum aestivum

Project Number

2017-SIUZIR-49

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