Detection of Five Parasites in Renal Transplantation Patients by Molecular Methods / Renal Transplantasyon Hastalarında Beş Parazitin Moleküler Yöntemler ile Tespiti

Özer Akgül; Hayriye Kırkoyun Uysal; İlker Tosun; Burcu Sapmaz; Reyhan Çalışkan; A. Canan Yazıcı Güvercin; Sinem Öktem; Özgür Kurt; Y. Ali Öner

Detection of Five Parasites in Renal Transplantation Patients by Molecular Methods / Renal Transplantasyon Hastalarında Beş Parazitin Moleküler Yöntemler ile Tespiti

Abstract

Objective: Intestinal parasitic infections are among the prominent public health concerns in
patients with suppressed immune function.
Material and methods: Here we examined stool specimens by microscopy and polymerase
chain reaction (PCR) to evaluate the infection of five principal protozoans (Cryptosporidium
spp., Giardia spp., Entamoeba histolytica, Blastocystis spp. and Dientamoeba spp.) among 90
renal transplant recipient patients (RTP) in comparison with 90 healthy individuals (HI) from
Turkey.
Results: The overall frequency of any parasites was 17.2% (31/180) with microscopy and
51.7% (93/180) with PCR. Because of its high sensitivity, PCR was compared with microscopy
in terms of the accuracy of detecting intestinal parasites, and the agreement was found to be
inadequate (κ= 0.217; p<0.001). Multiparasitism (90.9%), Cryptosporidium spp. (84.6%) and
Giardia spp. (74.1%) were the most frequent agents in RTP, respectively (p<0.001).
Conclusions: This is the first study performed in Turkish reporting the prevalence of five
intestinal parasites with PCR techniques among this group and seeks to provide a basis for
future studies

Keywords

Intestinal parasites,Transplantation,PCR,Microscopy

References

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Details

Primary Language

English

Subjects

-

Journal Section

Research Article

Authors

Özer Akgül ^* This is me

Hayriye Kırkoyun Uysal This is me

İlker Tosun This is me

Burcu Sapmaz This is me

Reyhan Çalışkan This is me

A. Canan Yazıcı Güvercin This is me

Sinem Öktem This is me

Özgür Kurt This is me

Y. Ali Öner This is me

Publication Date

January 31, 2019

Submission Date

December 3, 2018

Acceptance Date

December 25, 2018

Published in Issue

Year 2019 Volume: 2 Number: 1

IZ

https://izlik.org/JA97ZD53TT

Cite

RIS / Bibtex

APA

Akgül, Ö., Kırkoyun Uysal, H., Tosun, İ., Sapmaz, B., Çalışkan, R., Yazıcı Güvercin, A. C., Öktem, S., Kurt, Ö., & Öner, Y. A. (2019). Detection of Five Parasites in Renal Transplantation Patients by Molecular Methods / Renal Transplantasyon Hastalarında Beş Parazitin Moleküler Yöntemler ile Tespiti. Tıp Fakültesi Klinikleri Dergisi, 2(1), 17-23. https://izlik.org/JA97ZD53TT

AMA

1.Akgül Ö, Kırkoyun Uysal H, Tosun İ, et al. Detection of Five Parasites in Renal Transplantation Patients by Molecular Methods / Renal Transplantasyon Hastalarında Beş Parazitin Moleküler Yöntemler ile Tespiti. Tıp Fakültesi Klinikleri Dergisi. 2019;2(1):17-23. https://izlik.org/JA97ZD53TT

Chicago

Akgül, Özer, Hayriye Kırkoyun Uysal, İlker Tosun, et al. 2019. “Detection of Five Parasites in Renal Transplantation Patients by Molecular Methods Renal Transplantasyon Hastalarında Beş Parazitin Moleküler Yöntemler Ile Tespiti”. Tıp Fakültesi Klinikleri Dergisi 2 (1): 17-23. https://izlik.org/JA97ZD53TT.

EndNote

Akgül Ö, Kırkoyun Uysal H, Tosun İ, Sapmaz B, Çalışkan R, Yazıcı Güvercin AC, Öktem S, Kurt Ö, Öner YA (January 1, 2019) Detection of Five Parasites in Renal Transplantation Patients by Molecular Methods / Renal Transplantasyon Hastalarında Beş Parazitin Moleküler Yöntemler ile Tespiti. Tıp Fakültesi Klinikleri Dergisi 2 1 17–23.

IEEE

[1]Ö. Akgül et al., “Detection of Five Parasites in Renal Transplantation Patients by Molecular Methods / Renal Transplantasyon Hastalarında Beş Parazitin Moleküler Yöntemler ile Tespiti”, Tıp Fakültesi Klinikleri Dergisi, vol. 2, no. 1, pp. 17–23, Jan. 2019, [Online]. Available: https://izlik.org/JA97ZD53TT

ISNAD

Akgül, Özer - Kırkoyun Uysal, Hayriye - Tosun, İlker - Sapmaz, Burcu - Çalışkan, Reyhan - Yazıcı Güvercin, A. Canan - Öktem, Sinem - Kurt, Özgür - Öner, Y. Ali. “Detection of Five Parasites in Renal Transplantation Patients by Molecular Methods Renal Transplantasyon Hastalarında Beş Parazitin Moleküler Yöntemler Ile Tespiti”. Tıp Fakültesi Klinikleri Dergisi 2/1 (January 1, 2019): 17-23. https://izlik.org/JA97ZD53TT.

JAMA

1.Akgül Ö, Kırkoyun Uysal H, Tosun İ, Sapmaz B, Çalışkan R, Yazıcı Güvercin AC, Öktem S, Kurt Ö, Öner YA. Detection of Five Parasites in Renal Transplantation Patients by Molecular Methods / Renal Transplantasyon Hastalarında Beş Parazitin Moleküler Yöntemler ile Tespiti. Tıp Fakültesi Klinikleri Dergisi. 2019;2:17–23.

MLA

Akgül, Özer, et al. “Detection of Five Parasites in Renal Transplantation Patients by Molecular Methods Renal Transplantasyon Hastalarında Beş Parazitin Moleküler Yöntemler Ile Tespiti”. Tıp Fakültesi Klinikleri Dergisi, vol. 2, no. 1, Jan. 2019, pp. 17-23, https://izlik.org/JA97ZD53TT.

Vancouver

1.Özer Akgül, Hayriye Kırkoyun Uysal, İlker Tosun, Burcu Sapmaz, Reyhan Çalışkan, A. Canan Yazıcı Güvercin, Sinem Öktem, Özgür Kurt, Y. Ali Öner. Detection of Five Parasites in Renal Transplantation Patients by Molecular Methods / Renal Transplantasyon Hastalarında Beş Parazitin Moleküler Yöntemler ile Tespiti. Tıp Fakültesi Klinikleri Dergisi [Internet]. 2019 Jan. 1;2(1):17-23. Available from: https://izlik.org/JA97ZD53TT