Detection of Five Parasites in Renal Transplantation Patients by Molecular Methods / Renal Transplantasyon Hastalarında Beş Parazitin Moleküler Yöntemler ile Tespiti

Özer Akgül; Hayriye Kırkoyun Uysal; İlker Tosun; Burcu Sapmaz; Reyhan Çalışkan; A. Canan Yazıcı Güvercin; Sinem Öktem; Özgür Kurt; Y. Ali Öner

Detection of Five Parasites in Renal Transplantation Patients by Molecular Methods / Renal Transplantasyon Hastalarında Beş Parazitin Moleküler Yöntemler ile Tespiti

Öz

Objective: Intestinal parasitic infections are among the prominent public health concerns in
patients with suppressed immune function.
Material and methods: Here we examined stool specimens by microscopy and polymerase
chain reaction (PCR) to evaluate the infection of five principal protozoans (Cryptosporidium
spp., Giardia spp., Entamoeba histolytica, Blastocystis spp. and Dientamoeba spp.) among 90
renal transplant recipient patients (RTP) in comparison with 90 healthy individuals (HI) from
Turkey.
Results: The overall frequency of any parasites was 17.2% (31/180) with microscopy and
51.7% (93/180) with PCR. Because of its high sensitivity, PCR was compared with microscopy
in terms of the accuracy of detecting intestinal parasites, and the agreement was found to be
inadequate (κ= 0.217; p<0.001). Multiparasitism (90.9%), Cryptosporidium spp. (84.6%) and
Giardia spp. (74.1%) were the most frequent agents in RTP, respectively (p<0.001).
Conclusions: This is the first study performed in Turkish reporting the prevalence of five
intestinal parasites with PCR techniques among this group and seeks to provide a basis for
future studies

Anahtar Kelimeler

Intestinal parasites,Transplantation,PCR,Microscopy

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

-

Bölüm

Araştırma Makalesi

Yazarlar

Özer Akgül ^* Bu kişi benim

Hayriye Kırkoyun Uysal Bu kişi benim

İlker Tosun Bu kişi benim

Burcu Sapmaz Bu kişi benim

Reyhan Çalışkan Bu kişi benim

A. Canan Yazıcı Güvercin Bu kişi benim

Sinem Öktem Bu kişi benim

Özgür Kurt Bu kişi benim

Y. Ali Öner Bu kişi benim

Yayımlanma Tarihi

31 Ocak 2019

Gönderilme Tarihi

3 Aralık 2018

Kabul Tarihi

25 Aralık 2018

Yayımlandığı Sayı

Yıl 2019 Cilt: 2 Sayı: 1

IZ

https://izlik.org/JA97ZD53TT

Kaynak Göster

RIS / Bibtex

APA

Akgül, Ö., Kırkoyun Uysal, H., Tosun, İ., Sapmaz, B., Çalışkan, R., Yazıcı Güvercin, A. C., Öktem, S., Kurt, Ö., & Öner, Y. A. (2019). Detection of Five Parasites in Renal Transplantation Patients by Molecular Methods / Renal Transplantasyon Hastalarında Beş Parazitin Moleküler Yöntemler ile Tespiti. Tıp Fakültesi Klinikleri Dergisi, 2(1), 17-23. https://izlik.org/JA97ZD53TT

AMA

1.Akgül Ö, Kırkoyun Uysal H, Tosun İ, vd. Detection of Five Parasites in Renal Transplantation Patients by Molecular Methods / Renal Transplantasyon Hastalarında Beş Parazitin Moleküler Yöntemler ile Tespiti. TFK. 2019;2(1):17-23. https://izlik.org/JA97ZD53TT

Chicago

Akgül, Özer, Hayriye Kırkoyun Uysal, İlker Tosun, vd. 2019. “Detection of Five Parasites in Renal Transplantation Patients by Molecular Methods / Renal Transplantasyon Hastalarında Beş Parazitin Moleküler Yöntemler ile Tespiti”. Tıp Fakültesi Klinikleri Dergisi 2 (1): 17-23. https://izlik.org/JA97ZD53TT.

EndNote

Akgül Ö, Kırkoyun Uysal H, Tosun İ, Sapmaz B, Çalışkan R, Yazıcı Güvercin AC, Öktem S, Kurt Ö, Öner YA (01 Ocak 2019) Detection of Five Parasites in Renal Transplantation Patients by Molecular Methods / Renal Transplantasyon Hastalarında Beş Parazitin Moleküler Yöntemler ile Tespiti. Tıp Fakültesi Klinikleri Dergisi 2 1 17–23.

IEEE

[1]Ö. Akgül vd., “Detection of Five Parasites in Renal Transplantation Patients by Molecular Methods / Renal Transplantasyon Hastalarında Beş Parazitin Moleküler Yöntemler ile Tespiti”, TFK, c. 2, sy 1, ss. 17–23, Oca. 2019, [çevrimiçi]. Erişim adresi: https://izlik.org/JA97ZD53TT

ISNAD

Akgül, Özer - Kırkoyun Uysal, Hayriye - Tosun, İlker - Sapmaz, Burcu - Çalışkan, Reyhan - Yazıcı Güvercin, A. Canan - Öktem, Sinem - Kurt, Özgür - Öner, Y. Ali. “Detection of Five Parasites in Renal Transplantation Patients by Molecular Methods / Renal Transplantasyon Hastalarında Beş Parazitin Moleküler Yöntemler ile Tespiti”. Tıp Fakültesi Klinikleri Dergisi 2/1 (01 Ocak 2019): 17-23. https://izlik.org/JA97ZD53TT.

JAMA

1.Akgül Ö, Kırkoyun Uysal H, Tosun İ, Sapmaz B, Çalışkan R, Yazıcı Güvercin AC, Öktem S, Kurt Ö, Öner YA. Detection of Five Parasites in Renal Transplantation Patients by Molecular Methods / Renal Transplantasyon Hastalarında Beş Parazitin Moleküler Yöntemler ile Tespiti. TFK. 2019;2:17–23.

MLA

Akgül, Özer, vd. “Detection of Five Parasites in Renal Transplantation Patients by Molecular Methods / Renal Transplantasyon Hastalarında Beş Parazitin Moleküler Yöntemler ile Tespiti”. Tıp Fakültesi Klinikleri Dergisi, c. 2, sy 1, Ocak 2019, ss. 17-23, https://izlik.org/JA97ZD53TT.

Vancouver

1.Özer Akgül, Hayriye Kırkoyun Uysal, İlker Tosun, Burcu Sapmaz, Reyhan Çalışkan, A. Canan Yazıcı Güvercin, Sinem Öktem, Özgür Kurt, Y. Ali Öner. Detection of Five Parasites in Renal Transplantation Patients by Molecular Methods / Renal Transplantasyon Hastalarında Beş Parazitin Moleküler Yöntemler ile Tespiti. TFK [Internet]. 01 Ocak 2019;2(1):17-23. Erişim adresi: https://izlik.org/JA97ZD53TT