Investigation of the Effects of Sodium Pentaborate Pentahydrate on MC3T3-E1 Mouse Pre-osteoblast Cell Line
Abstract
Boron compounds have been investigated in many studies for their positive effects on human health, antimicrobial properties and their effects on cell proliferation and differentiation. In this study, the effects of sodium pentaborate pentahydrate on the viability and osteogenic differentiation of MC3T3-E1 mouse osteoblast cell line were investigated. The effect of SPP on cell viability was examined by MTT and resazurin analyses. The osteogenic differentiation properties of cells with and without SPP treatment at selected concentrations were examined by invert phase contrast microscope images and alkaline phosphatase (ALP) staining methods. It was determined that the viability of MC3T3-E1 cells was not affected up to 2 mg/mL SPP in the culture medium after 24 hours of culture, while 0.25 mg/mL SPP significantly increased cell viability compared to the control group. Therefore, SPP concentrations of 0.125, 0.25 and 0.5 mg/mL were selected to understand the effect of SPP on osteogenic differentiation of MC3T3-E1 cells at days 7 and 14. Invert phase contrast images and ALP staining indicated that osteogenic differentiation capacity of MC3T3-E1 cell line was suppressed in the presence of SPP. There was no ALP stain in osteogenically induced cells in the presence of SPP. These findings showed that 0.125, 0.25 and 0.5 mg/mL SPP suppressed osteogenic differentiation in long-term cultures, although it had no negative effect on cell viability at 24 hours.
Keywords
Supporting Institution
Scientific Research Projects Coordination Unit of Adana Alparslan Türkeş Science and Technology University (BAP)
Project Number
24303003
Thanks
The authors would like to thank Scientific Research Projects Coordination Unit of Adana Alparslan Türkeş Science and Technology University (BAP)
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