TNF-a TAYİNİ İÇİN REFLEKTOMETRİK İNTERFERANS SPEKTROSKOPİSİ TEMELLİ SENSÖR GELİŞTİRİLMESİ

Year 2010, Volume: 11 Issue: 2, 141 - 147, 22.10.2010

Sibel Emir Diltemiz , Ayça Atılır Özcan , Özlem Yalçınkaya

Abstract

In this work, sensor systems based on reflectometric interference spectroscopy has been prepared for selectively recognition and binding of TNF-a biomolecules. For this purpose, carboxymethyldextran coated and reflectometric interference spectroscopy based sensor systems have been activated for binding of suitable orientated antibody, then anti-TNF-a antibody has been bounded on the surface and binding efficiency and binding constants of prepared sensors have been investigated

Keywords

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TNF-α TAYİNİ İÇİN REFLEKTOMETRİK İNTERFERANS SPEKTROSKOPİSİ TEMELLİ SENSÖR GELİŞTİRİLMESİ

Abstract

Bu çalışmada, TNF-α biyomolekülünü seçici olarak tanıyan ve bağlayabilen reflektometrik interferans spektroskopisi temelli sensör sistemi hazırlanmıştır. Bu amaçla, reflektometrik interferans spektroskopisi temelli ve karboksimetildekstran kaplı sensör sistemleri TNF-α antibadinin uygun yönlenimli bağlanabilmesi amacıyla aktive edilmiş, ardından uygun derişimdeki anti-TNF-α antibadi aktive yüzeylere bağlanmış ve hazırlanan sensörlerin TNF-α molekülünü bağlama etkinlikleri incelenerek, bağlanma sabitleri hesaplanmıştır.

Keywords

TNF-α, Reflektometrik interferans spektroskopisi, Biyosensör sistemleri

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